FastQCFastQC Report
Mon 6 Jun 2016
ERR1211496_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211496_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084153
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254960.35990188241275983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233590.32973596137745753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150970.21310945712211468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123800.049.4224471
GTATCAA207750.037.1706161
TACCTGG62100.032.5638122
GTACCTG70400.029.7272661
TCAACGC258500.029.3588624
ATCAACG260400.029.0548233
CAACGCA263750.028.792085
AACGCAG272350.027.9340846
TATCAAC273200.027.8864172
ACCTGGG77800.025.1273733
ACGCAGA312950.024.0848737
GTACATG224350.024.0019781
CGCAGAG316950.023.5881778
TACATGG230150.023.1105372
ACATGGG226550.022.1326643
GCAGAGT349900.020.8965449
GAGTACT216200.020.64808712-13
CATGGGG129900.020.113744
AGAGTAC319550.019.6093410-11
AGTACTT228800.018.97701312-13
Produced by FastQC (version 0.11.5)