FastQCFastQC Report
Mon 6 Jun 2016
ERR1211495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7708930
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242770.31492048831679625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218230.2830872767037708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130910.1698160445094196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132950.049.1553541
GTATCAA222150.036.751281
TCAACGC259400.030.6309534
ATCAACG263400.030.1849983
CAACGCA264150.030.0082055
TATCAAC276350.028.9950562
AACGCAG275300.028.9400566
TACCTGG69600.027.6329922
GTACCTG75650.026.8248961
GTACATG251650.025.950711
ACGCAGA310750.025.3824427
CGCAGAG317350.024.796478
TACATGG258850.024.760622
ACATGGG254200.024.234483
CATGGGG145950.022.1176764
GCAGAGT351600.021.7551169
ACCTGGG86750.021.6669163
GAGTACT207700.020.93711912-13
CAGAGTA341100.019.78010410-11
AGAGTAC319050.018.6804610-11
Produced by FastQC (version 0.11.5)