FastQCFastQC Report
Mon 6 Jun 2016
ERR1211495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7708930
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264660.34331612817861884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245310.31821536841040193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158710.20587811797486813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144750.045.939441
GTATCAA235600.035.849731
TCAACGC285550.029.079954
GTACCTG73800.028.994121
ATCAACG287600.028.8399893
CAACGCA288500.028.8314675
TACCTGG72100.028.8294432
AACGCAG298400.027.905886
TATCAAC303100.027.5243382
GTACATG259800.024.889631
ACGCAGA337650.024.6338167
CGCAGAG343450.024.0809928
TACATGG266750.023.6943112
ACCTGGG87050.023.2134113
ACATGGG264250.023.0300053
CATGGGG146700.022.199464
GCAGAGT382850.021.1852959
GAGTACT231050.020.73538412-13
AGAGTAC350450.019.13476410-11
CAGAGTA372600.018.89270610-11
Produced by FastQC (version 0.11.5)