##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7708930 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12343943971472 33.0 31.0 34.0 30.0 34.0 2 32.3394875812856 34.0 31.0 34.0 30.0 34.0 3 32.35381006183738 34.0 31.0 34.0 30.0 34.0 4 35.98682372261779 37.0 35.0 37.0 35.0 37.0 5 35.907413480210614 37.0 35.0 37.0 35.0 37.0 6 35.92682810714327 37.0 35.0 37.0 35.0 37.0 7 35.85566725862085 37.0 35.0 37.0 35.0 37.0 8 35.849505573406425 37.0 35.0 37.0 35.0 37.0 9 37.508375740861574 39.0 37.0 39.0 35.0 39.0 10-11 37.47294592115897 39.0 37.0 39.0 34.5 39.0 12-13 37.387075443673766 39.0 37.0 39.0 34.0 39.0 14-15 38.62867071046176 40.0 38.0 41.0 34.0 41.0 16-17 38.55393835460953 40.0 38.0 41.0 34.0 41.0 18-19 38.54519239375634 40.0 38.0 41.0 34.0 41.0 20-21 38.48773273333653 40.0 38.0 41.0 34.0 41.0 22-23 38.38358754068334 40.0 38.0 41.0 34.0 41.0 24-25 38.27723127593583 40.0 38.0 41.0 34.0 41.0 26-27 38.10337738181563 40.0 38.0 41.0 33.0 41.0 28-29 38.142795563067764 40.0 38.0 41.0 33.0 41.0 30-31 38.07193242123096 40.0 37.5 41.0 33.0 41.0 32-33 37.89497913458807 40.0 37.0 41.0 33.0 41.0 34-35 37.785763718181386 40.0 37.0 41.0 33.0 41.0 36-37 37.73441638463444 40.0 37.0 41.0 33.0 41.0 38-39 37.645141867937575 40.0 37.0 41.0 32.5 41.0 40-41 37.48943400705416 40.0 37.0 41.0 32.0 41.0 42-43 37.34196347612444 40.0 36.0 41.0 31.0 41.0 44-45 37.163919182039535 40.0 36.0 41.0 31.0 41.0 46-47 36.91387987697385 39.0 36.0 41.0 30.0 41.0 48-49 36.69664784866382 39.0 35.5 41.0 30.0 41.0 50-51 36.502239156406915 39.0 35.0 41.0 29.5 41.0 52-53 36.30319888752395 39.0 35.0 40.0 29.0 41.0 54-55 36.11308469528197 39.0 35.0 40.0 28.0 41.0 56-57 35.91778807175574 38.5 35.0 40.0 28.0 41.0 58-59 35.66083023195178 38.0 34.0 40.0 28.0 41.0 60-61 35.35432744881585 38.0 34.0 40.0 27.0 41.0 62-63 35.03896844309132 37.5 34.0 40.0 26.0 41.0 64-65 34.72566627275121 37.0 33.5 40.0 26.0 41.0 66-67 34.38561752409219 36.5 33.0 39.0 26.0 41.0 68-69 33.98970427802561 36.0 32.5 39.0 25.5 40.0 70-71 33.6006557330265 35.5 32.0 39.0 25.0 40.0 72-73 33.189164254961455 35.0 32.0 38.0 24.0 40.0 74-75 32.78044644328072 35.0 31.0 37.0 23.5 39.0 76-77 31.22064268322582 33.5 29.5 35.5 21.5 38.5 78-79 32.05780925498091 34.0 31.0 36.0 23.0 39.0 80-81 32.02257291219404 34.5 31.0 36.0 23.0 37.5 82-83 31.730574346893796 34.0 31.0 35.5 22.5 37.0 84-85 31.427528269163165 34.0 31.0 35.0 21.5 37.0 86-87 31.12853048347825 34.0 30.0 35.0 20.0 36.0 88-89 30.845482057821254 34.0 30.0 35.0 20.0 36.0 90-91 30.560718543299785 34.0 29.5 35.0 19.5 35.5 92-93 30.34701411220494 34.0 29.0 35.0 19.0 35.0 94-95 30.125139091936234 34.0 29.0 35.0 18.0 35.0 96-97 29.91908766845723 34.0 29.0 35.0 18.0 35.0 98-99 29.75498279268329 34.0 29.0 35.0 18.0 35.0 100 27.878898368515475 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29093526453991103 1101 2 0.2351052322377143 1101 3 0.27786876914664305 1101 4 0.1518161407938976 1101 5 0.17210435601908358 1101 6 0.17890520575431168 1101 7 0.24048687363416832 1101 8 0.15581640481256187 1101 9 0.27024831629436363 1101 10-11 0.22758033007716705 1101 12-13 0.21325281988205091 1101 14-15 0.30777328653111624 1101 16-17 0.3303137760151955 1101 18-19 0.3340454622976168 1101 20-21 0.23329166642634647 1101 22-23 0.26976593130079607 1101 24-25 0.31140792908764325 1101 26-27 0.2387523393620441 1101 28-29 0.3474906041472252 1101 30-31 0.34565743762591694 1101 32-33 0.3751609110648104 1101 34-35 0.3471678717498321 1101 36-37 0.36870582132615226 1101 38-39 0.386639675675049 1101 40-41 0.3719497803107501 1101 42-43 0.3541107947530975 1101 44-45 0.3863717313101631 1101 46-47 0.4362644754547631 1101 48-49 0.4345283504646318 1101 50-51 0.4012330714480896 1101 52-53 0.36863519236637643 1101 54-55 0.3274116987039548 1101 56-57 0.339796386152976 1101 58-59 0.43115645245422485 1101 60-61 0.3543610422222656 1101 62-63 0.36755398921527416 1101 64-65 0.39231269218624476 1101 66-67 0.23008869899647522 1101 68-69 0.27454422946487966 1101 70-71 0.3223003130548747 1101 72-73 0.24278930878126204 1101 74-75 0.2718368307523633 1101 76-77 0.2672526666107835 1101 78-79 0.3075526207706716 1101 80-81 0.3155657729219783 1101 82-83 0.3552928031110163 1101 84-85 0.3037248426161767 1101 86-87 0.29215461473788906 1101 88-89 0.16187645607754675 1101 90-91 0.136905067733327 1101 92-93 0.0966196090837812 1101 94-95 0.08261278963518492 1101 96-97 0.06471614368546952 1101 98-99 -0.08329929147591031 1101 100 -0.019245740449328252 1102 1 0.32956154760766765 1102 2 0.24929613399459072 1102 3 0.33915023871017524 1102 4 0.2105614751324083 1102 5 0.19910517205349976 1102 6 0.17595526016328478 1102 7 0.18460182441675244 1102 8 0.16226118657721855 1102 9 0.18843126204271954 1102 10-11 0.1935034248602605 1102 12-13 0.2557139200925036 1102 14-15 0.3050874902685905 1102 16-17 0.3697554127356355 1102 18-19 0.33479586316681775 1102 20-21 0.3490354709706267 1102 22-23 0.34713510143141235 1102 24-25 0.3002498858306879 1102 26-27 0.24632308283435123 1102 28-29 0.2956980007609076 1102 30-31 0.37755104966515063 1102 32-33 0.38222541393081144 1102 34-35 0.376472496006258 1102 36-37 0.43291928970361226 1102 38-39 0.36812101774104633 1102 40-41 0.4029245493980014 1102 42-43 0.3812131245397623 1102 44-45 0.31642817535910694 1102 46-47 0.34062713474292394 1102 48-49 0.3593623023978125 1102 50-51 0.40905191292910104 1102 52-53 0.3366675640541317 1102 54-55 0.2908560425645774 1102 56-57 0.36575508109483934 1102 58-59 0.39891709965838373 1102 60-61 0.39650835748452806 1102 62-63 0.4154171427505915 1102 64-65 0.3149360203161464 1102 66-67 0.2831255099253269 1102 68-69 0.31885512915329883 1102 70-71 0.316120688414685 1102 72-73 0.34866787873827576 1102 74-75 0.27212821059429615 1102 76-77 0.3160866613652793 1102 78-79 0.35891492865172125 1102 80-81 0.3266609590771097 1102 82-83 0.2961047994015793 1102 84-85 0.26810047003590753 1102 86-87 0.2726158870878983 1102 88-89 0.26170217678714636 1102 90-91 0.26852337394253567 1102 92-93 0.2072445776652394 1102 94-95 0.22069348400473388 1102 96-97 0.24215844509740592 1102 98-99 0.2063063757171868 1102 100 0.26754634729251237 1103 1 0.26095016211235844 1103 2 0.2378288147010963 1103 3 0.27539789147422766 1103 4 0.11719139038719817 1103 5 0.13161080305508221 1103 6 0.1844927758486392 1103 7 0.19053248250510535 1103 8 0.18549083476431605 1103 9 0.2232065119663602 1103 10-11 0.19668902810580136 1103 12-13 0.22612323910728804 1103 14-15 0.3346944216661143 1103 16-17 0.3234161934351434 1103 18-19 0.3244391261968147 1103 20-21 0.2736037929283057 1103 22-23 0.29190595796947605 1103 24-25 0.2964223225577385 1103 26-27 0.2537308786548209 1103 28-29 0.30071140418896647 1103 30-31 0.38122356035770366 1103 32-33 0.3756452755311983 1103 34-35 0.42204573246257127 1103 36-37 0.4613482174392658 1103 38-39 0.4858860372189966 1103 40-41 0.44541481317381226 1103 42-43 0.4902547247153919 1103 44-45 0.4251168171293074 1103 46-47 0.5219288191655309 1103 48-49 0.5456152482318686 1103 50-51 0.5740290820621823 1103 52-53 0.5545069669760423 1103 54-55 0.5835695226637867 1103 56-57 0.6110296481712183 1103 58-59 0.6152158892528874 1103 60-61 0.6434355035040724 1103 62-63 0.6705961161578813 1103 64-65 0.6443013704996048 1103 66-67 0.6665472517341584 1103 68-69 0.5953111028543603 1103 70-71 0.6345823418960563 1103 72-73 0.5438052356920267 1103 74-75 0.47732603008042673 1103 76-77 0.564485143362301 1103 78-79 0.5833905077740695 1103 80-81 0.6068971883787313 1103 82-83 0.5810431721188358 1103 84-85 0.5027708731792124 1103 86-87 0.45874674860612075 1103 88-89 0.4648332655361749 1103 90-91 0.45802251148833406 1103 92-93 0.465156961161334 1103 94-95 0.507888106861536 1103 96-97 0.4736804614639638 1103 98-99 0.5375055203130792 1103 100 0.5784068910384548 1104 1 0.2496599609946557 1104 2 0.22253017040448242 1104 3 0.2977575539274895 1104 4 0.08108789672945704 1104 5 0.1424231557132387 1104 6 0.15978908956707727 1104 7 0.18343689391507922 1104 8 0.17636402624678027 1104 9 0.21716069697852447 1104 10-11 0.20587647048008506 1104 12-13 0.2667428560326286 1104 14-15 0.36548585849838844 1104 16-17 0.27506533156940094 1104 18-19 0.2912256322747311 1104 20-21 0.18471024120194812 1104 22-23 0.3614411232369861 1104 24-25 0.29528996244538774 1104 26-27 0.3647693693353915 1104 28-29 0.3947473507026018 1104 30-31 0.3243921372668126 1104 32-33 0.3327249762808435 1104 34-35 0.4300117409055204 1104 36-37 0.3186026872878216 1104 38-39 0.41207579966616237 1104 40-41 0.4661254431167663 1104 42-43 0.48615777865776977 1104 44-45 0.442053032310298 1104 46-47 0.3787723333439956 1104 48-49 0.4226418720901961 1104 50-51 0.37676654225836614 1104 52-53 0.4164683326468932 1104 54-55 0.4782244198154686 1104 56-57 0.5037601054995093 1104 58-59 0.552010246330461 1104 60-61 0.3992426868690231 1104 62-63 0.45491214569992877 1104 64-65 0.6125170328528426 1104 66-67 0.5419936199745337 1104 68-69 0.5840923691850364 1104 70-71 0.41857982958759266 1104 72-73 0.5198109070130172 1104 74-75 0.5784652657409808 1104 76-77 0.42837043501029015 1104 78-79 0.5912739368436206 1104 80-81 0.5922401310745471 1104 82-83 0.49880862523102465 1104 84-85 0.5388666805535571 1104 86-87 0.5832089514181504 1104 88-89 0.6187406779794848 1104 90-91 0.4733571107484984 1104 92-93 0.45637569996911864 1104 94-95 0.5063017707296993 1104 96-97 0.4379323210307007 1104 98-99 0.51771528177024 1104 100 0.7137624415227926 1105 1 0.19471633181888848 1105 2 0.1476796951978585 1105 3 0.1796283073764684 1105 4 0.09420509244662156 1105 5 0.08304179567798542 1105 6 0.09000853229562722 1105 7 0.12050167568045822 1105 8 0.09173260440504549 1105 9 0.1717028899341173 1105 10-11 0.15363127154556366 1105 12-13 0.165384227002086 1105 14-15 0.2211399019685274 1105 16-17 0.231318866431117 1105 18-19 0.2600251603614012 1105 20-21 0.20903030419081148 1105 22-23 0.2959968678775482 1105 24-25 0.26543836260099596 1105 26-27 0.29543944618354345 1105 28-29 0.2943311060847975 1105 30-31 0.345147142848262 1105 32-33 0.31594331678434173 1105 34-35 0.40459248315043084 1105 36-37 0.41773051554883267 1105 38-39 0.42403561762235853 1105 40-41 0.3874271287126376 1105 42-43 0.37157547209718444 1105 44-45 0.420894755182168 1105 46-47 0.4351709447622696 1105 48-49 0.4413350512965053 1105 50-51 0.5892305275492333 1105 52-53 0.5696346482192283 1105 54-55 0.5866029297201649 1105 56-57 0.5308780837175604 1105 58-59 0.5228523255758617 1105 60-61 0.5555311372451044 1105 62-63 0.47949801656749713 1105 64-65 0.5960453134046233 1105 66-67 0.5330526782656477 1105 68-69 0.5914293227237195 1105 70-71 0.5661000504831364 1105 72-73 0.5451223178953981 1105 74-75 0.6158849430482789 1105 76-77 0.519852270194999 1105 78-79 0.5951221405335261 1105 80-81 0.6572812006502318 1105 82-83 0.6892650088968288 1105 84-85 0.6325666164486314 1105 86-87 0.623638878652315 1105 88-89 0.6272560069008684 1105 90-91 0.6843104250737184 1105 92-93 0.6843694062285977 1105 94-95 0.7578683877072336 1105 96-97 0.6813891676447454 1105 98-99 0.6487730956413742 1105 100 0.8996906787700176 1106 1 0.21958824832742607 1106 2 0.16487202374217702 1106 3 0.2286589841633173 1106 4 0.1128253147290792 1106 5 0.0985999866963283 1106 6 0.08196886481572818 1106 7 0.10765560176702138 1106 8 0.11151212911815378 1106 9 0.13189445042928583 1106 10-11 0.11068930443614278 1106 12-13 0.1609976497905592 1106 14-15 0.24132502440460257 1106 16-17 0.3163046349320595 1106 18-19 0.3052119770235535 1106 20-21 0.28658711723861074 1106 22-23 0.2771218339930215 1106 24-25 0.2699610426407517 1106 26-27 0.2607964760922812 1106 28-29 0.2395889672937912 1106 30-31 0.3205624541340626 1106 32-33 0.3664001930832441 1106 34-35 0.3890170110261053 1106 36-37 0.3524938886400051 1106 38-39 0.32539992140838336 1106 40-41 0.32327299931894515 1106 42-43 0.3489357549199852 1106 44-45 0.40925948817213964 1106 46-47 0.5266523760444599 1106 48-49 0.4627502948132616 1106 50-51 0.462480053165919 1106 52-53 0.4933610420025403 1106 54-55 0.3867978786674868 1106 56-57 0.4642551280836855 1106 58-59 0.5575013948810081 1106 60-61 0.6256059090893231 1106 62-63 0.5227893330941171 1106 64-65 0.598712251147461 1106 66-67 0.5596865898948167 1106 68-69 0.49481336989097 1106 70-71 0.5455795842321223 1106 72-73 0.5874426169827771 1106 74-75 0.49106080371512206 1106 76-77 0.44360068891732496 1106 78-79 0.5858560792703145 1106 80-81 0.6051598037043533 1106 82-83 0.5938920532629588 1106 84-85 0.515759554914343 1106 86-87 0.45180527424280825 1106 88-89 0.5627512856575336 1106 90-91 0.556441422634034 1106 92-93 0.6108074653232052 1106 94-95 0.6660298135679028 1106 96-97 0.6440533946258604 1106 98-99 0.6885487801087109 1106 100 0.8102606164225001 1107 1 0.2533492583098109 1107 2 0.17892055155024877 1107 3 0.22687145235669703 1107 4 0.1407992682564725 1107 5 0.1688676516013956 1107 6 0.14095266091784708 1107 7 0.12453603685356995 1107 8 0.15360032082466546 1107 9 0.18449811902532076 1107 10-11 0.14537246160976025 1107 12-13 0.21132822888068148 1107 14-15 0.2593461286184535 1107 16-17 0.20354039535484247 1107 18-19 0.14806740721676448 1107 20-21 0.18477857908465722 1107 22-23 0.3042017769508334 1107 24-25 0.23460229197666393 1107 26-27 0.2592774480465394 1107 28-29 0.34372085639706285 1107 30-31 0.21887712292583217 1107 32-33 0.20385423077583198 1107 34-35 0.29993055994537343 1107 36-37 0.31441825879910823 1107 38-39 0.3051766459561023 1107 40-41 0.29011634998863656 1107 42-43 0.29190324226724584 1107 44-45 0.3398763617565663 1107 46-47 0.33492559137874167 1107 48-49 0.44702094464689424 1107 50-51 0.3794295540467232 1107 52-53 0.38944512331563175 1107 54-55 0.4093131528335263 1107 56-57 0.49617837976838786 1107 58-59 0.5250365918723148 1107 60-61 0.4855658886771721 1107 62-63 0.426992489988109 1107 64-65 0.5602981429909804 1107 66-67 0.5453832596372834 1107 68-69 0.4641250887065098 1107 70-71 0.500157115479503 1107 72-73 0.4298396823855626 1107 74-75 0.5053663359691356 1107 76-77 0.48748329803848733 1107 78-79 0.556742435280718 1107 80-81 0.6046169241920083 1107 82-83 0.6361382644309295 1107 84-85 0.4921760677907763 1107 86-87 0.4254936532937812 1107 88-89 0.5087562698590844 1107 90-91 0.4266833276755584 1107 92-93 0.48640032666034116 1107 94-95 0.6501792137860036 1107 96-97 0.5747923566816162 1107 98-99 0.6328100627192264 1107 100 0.874124176526518 1108 1 0.24784111919689167 1108 2 0.18012203211205957 1108 3 0.19395525008003744 1108 4 0.1931409429724127 1108 5 0.10624216308879397 1108 6 0.18627196055602013 1108 7 0.19185053073462655 1108 8 0.18593265430033767 1108 9 0.18296618589638314 1108 10-11 0.20078705070596214 1108 12-13 0.21040788001810995 1108 14-15 0.2452507640231545 1108 16-17 0.2837788556960632 1108 18-19 0.3405850366059511 1108 20-21 0.31647670454763954 1108 22-23 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-0.33731535192620044 2316 80-81 -0.23748216965977775 2316 82-83 -0.16781392405630413 2316 84-85 -0.22703132641430201 2316 86-87 -0.22949497426526833 2316 88-89 -0.14853249044018924 2316 90-91 -0.09056285187988777 2316 92-93 -0.012473672563775295 2316 94-95 -0.011194158711198554 2316 96-97 0.023543121791991695 2316 98-99 -0.15046149710029866 2316 100 -0.31996343460916066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 7.0 10 5.0 11 35.0 12 71.0 13 241.0 14 762.0 15 1829.0 16 4240.0 17 8680.0 18 15459.0 19 23867.0 20 33961.0 21 46901.0 22 61303.0 23 70856.0 24 76434.0 25 83941.0 26 98023.0 27 117126.0 28 140978.0 29 170494.0 30 204694.0 31 248479.0 32 303276.0 33 380896.0 34 496874.0 35 683039.0 36 1001169.0 37 1574811.0 38 1608477.0 39 251968.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78904508961402 20.323114062073735 14.002673093714858 23.88516775459739 2 15.061158248672218 21.559006357923934 39.88333550984506 23.496499883558787 3 17.249004199575708 26.84794173571912 31.03643930358701 24.86661476111816 4 11.982102201344139 17.930682805974044 38.528977162833364 31.55823782984846 5 12.433305213994739 38.22222389419069 35.80465262412063 13.539818267693942 6 30.26554543275243 38.36710301148493 18.12282134595807 13.244530209804578 7 26.371870026060684 33.04885373197058 23.048892647877203 17.53038359409153 8 25.258754346948496 36.346819813471676 20.673184192310764 17.72124164726906 9 26.127530020378963 15.299710854813833 20.64683425585652 37.92592486895068 10-11 24.116751611442833 26.631737478482748 29.419666542568162 19.831844367506253 12-13 24.832207582634684 24.535921327603184 29.147884336736745 21.483986753025388 14-15 22.201706332785484 25.943872885082627 26.797908399739008 25.05651238239289 16-17 21.748499374259577 28.773863346334593 27.4730756495685 22.004561629837326 18-19 21.610708575903576 27.855272048053344 29.320458260340924 21.213561115702156 20-21 22.40481977099796 27.14807298021123 29.312585253088567 21.134521995702237 22-23 22.18637346557823 27.063113817352086 29.06116672482433 21.689345992245357 24-25 21.88168056570252 27.188871137465547 29.237982246039916 21.691466050792016 26-27 21.88898907589743 27.279078241470234 29.17697777781237 21.654954904819963 28-29 21.88924376207783 27.29745797593599 29.27616895734348 21.5371293046427 30-31 22.16335591811209 27.200336031308613 29.224417453521173 21.411890597058132 32-33 21.89295131291951 27.349553711923857 29.15015609639916 21.607338878757474 34-35 22.11224645664414 27.422290702580586 29.108961496662662 21.356501344112612 36-37 22.15172003873224 27.32446137857482 28.987341583316834 21.536476999376106 38-39 22.00427173584509 27.444754767597075 28.99548698177799 21.555486514779844 40-41 22.11157470393931 27.315516450158185 29.06565306860435 21.50725577729815 42-43 21.9585153319954 27.32799585905747 28.94152345690864 21.771965352038496 44-45 21.990184628153703 27.52511798307354 28.8162735756963 21.668423813076465 46-47 22.015864793538572 27.42553599721031 28.621388982834155 21.93721022641696 48-49 21.992364542500447 27.50471961981503 28.631019655828403 21.87189618185611 50-51 22.048795707461867 27.711396605719056 28.401857228479578 21.837950458339495 52-53 22.075430259486673 27.755505406685828 28.280924795202868 21.888139538624632 54-55 21.988548708527176 27.691940476634468 28.413063747468374 21.90644706736998 56-57 22.0759686482301 27.739140073782682 28.209970540502866 21.974920737484354 58-59 22.041577648829026 27.660763652727756 28.40482932120118 21.89282937724204 60-61 22.151593828581674 27.812740263258966 28.266897619935495 21.768768288223868 62-63 21.966053001366532 27.796720974581852 28.375015357741646 21.862210666309974 64-65 21.934086481931388 27.60759518959045 28.353487837505458 22.1048304909727 66-67 21.931210883873643 27.689611940004188 28.247840139862575 22.1313370362596 68-69 21.885927234724 27.72234401929385 28.263616447359503 22.12811229862265 70-71 21.920198658507626 27.52446340272617 28.264237246018343 22.29110069274786 72-73 21.833163960254158 27.760759028367822 28.085090042542944 22.320986968835072 74-75 21.86356161924144 27.84696659460952 27.98534927308069 22.304122513068357 76-77 21.767592919063635 27.705437416279636 28.14427287202067 22.382696792636057 78-79 21.73575462765695 27.673774825751174 28.10798851082823 22.482482035763653 80-81 21.751067075187837 27.883178650349343 27.882724603402963 22.48302967105985 82-83 21.818274170552748 27.799416500500218 27.94646553617693 22.4358437927701 84-85 21.782594999362964 27.83584544976386 27.883376593788604 22.498182957084573 86-87 21.67225517922965 27.812050297521314 27.99883070057151 22.516863822677532 88-89 21.651180013914114 27.66204091143934 27.98873844242367 22.698040632222877 90-91 21.795633337355635 27.735878801561693 27.899871935103405 22.56861592597927 92-93 21.716614218865804 27.68299195709551 27.88985046975283 22.710543354285853 94-95 21.546973263388082 27.65300989244454 27.923496335258108 22.876520508909273 96-97 21.562137431203567 27.589994911224085 27.963978421098933 22.88388923647342 98-99 21.501817120753323 27.559139298059833 27.832917267973123 23.106126313213725 100 20.740308739844256 27.978322300732017 28.06776811037618 23.21360084904755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3220.0 1 2310.5 2 2139.5 3 2901.0 4 2279.0 5 1904.5 6 2864.5 7 5555.5 8 8752.0 9 10496.5 10 9192.5 11 7073.5 12 7101.0 13 7631.0 14 8095.5 15 8642.0 16 9274.0 17 9937.5 18 10760.5 19 11699.5 20 12958.5 21 14930.5 22 17834.5 23 21948.0 24 26997.0 25 33985.5 26 42959.0 27 53410.0 28 65600.0 29 79452.5 30 95368.5 31 110967.5 32 128169.0 33 147845.0 34 168123.0 35 187176.0 36 207514.5 37 226207.5 38 239536.5 39 251766.5 40 259169.0 41 263559.0 42 268790.5 43 276354.0 44 283511.0 45 290074.5 46 294656.5 47 296118.5 48 298874.5 49 299075.5 50 294318.5 51 282366.0 52 265611.5 53 250866.5 54 236023.5 55 216453.0 56 192967.5 57 169615.0 58 147637.5 59 125120.5 60 102743.0 61 82702.0 62 65645.0 63 50027.0 64 36332.0 65 26306.5 66 19002.5 67 14049.0 68 10478.5 69 7589.0 70 5564.0 71 4128.5 72 3067.0 73 2228.0 74 1600.0 75 1098.0 76 728.0 77 467.0 78 300.0 79 186.0 80 141.5 81 119.0 82 79.5 83 53.5 84 40.0 85 27.0 86 23.0 87 15.0 88 10.0 89 9.0 90 6.0 91 3.5 92 3.5 93 4.0 94 2.5 95 1.5 96 2.5 97 1.5 98 2.0 99 4.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003359739937968045 2 0.07098261367012024 3 7.264302568579556E-4 4 6.356264747507112E-4 5 0.0015436642958231556 6 5.448226926434667E-4 7 0.0 8 9.599256965622985E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.107480545289684E-4 18-19 0.0010442434942333112 20-21 0.0034375717512028256 22-23 0.0 24-25 2.594393774492699E-5 26-27 1.6863559534202542E-4 28-29 9.469537276898351E-4 30-31 0.0016150101246217052 32-33 0.0030094967784115305 34-35 0.004053740272644842 36-37 0.0013815146849173621 38-39 0.0015566362646956193 40-41 0.005415797004253508 42-43 0.007004863191130287 44-45 0.005305535268837569 46-47 0.00701783516000275 48-49 0.008846882771020104 50-51 0.00869121914455054 52-53 0.0042288618524230995 54-55 0.0024971040079492224 56-57 0.0027305994476535655 58-59 0.00348945962669268 60-61 0.0076340036814447655 62-63 0.014152418039857671 64-65 0.004073198225953536 66-67 0.010273799346991087 68-69 0.012731987448322919 70-71 0.005519572755233217 72-73 0.0078026392767867915 74-75 0.003035440716156458 76-77 0.0030094967784115305 78-79 0.008152882436343305 80-81 0.0061746571832926225 82-83 0.01682464362758515 84-85 0.017538101915570644 86-87 0.022713917495683578 88-89 0.02137780470181984 90-91 0.02464674085768064 92-93 0.034978914064597806 94-95 0.03889644866408179 96-97 0.02298632884200531 98-99 0.018251560203556137 100 0.019068794242521336 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7708930.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.264440010592125 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8568322681228 47.35775574409612 2 16.040793895760295 20.296236862811988 3 4.829153189723967 9.165410168197544 4 1.761584720527083 4.457826835014453 5 0.8288275863396255 2.6217656557553553 6 0.466627243767088 1.771254676236653 7 0.27182691730318936 1.2037884392094267 8 0.18838032733765772 0.9534220734423162 9 0.13121009144438361 0.7470839663070762 >10 0.5781105654175679 6.253739857695718 >50 0.029915498193982024 1.2961543510467608 >100 0.015327710671014717 1.7839327892149857 >500 9.219195006801179E-4 0.39302150867022617 >1k 4.0672157560140115E-4 0.5515528343890421 >5k 0.0 0.0 >10k+ 8.134431503821702E-5 1.147054237912351 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26466 0.34331612817861884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24531 0.31821536841040193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15871 0.20587811797486813 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2971968872463493E-5 2 0.0 0.0 0.0 0.0 1.2971968872463493E-5 3 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 4 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 5 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 6 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 7 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 8 0.0 0.0 0.0 1.2971968872463493E-5 1.2971968872463493E-5 9 0.0 0.0 0.0 1.2971968872463493E-5 2.5943937744926987E-5 10-11 0.0 1.2971968872463493E-5 0.0 1.945795330869524E-5 2.5943937744926987E-5 12-13 0.0 1.2971968872463493E-5 0.0 3.242992218115873E-5 3.242992218115873E-5 14-15 0.0 3.242992218115873E-5 0.0 5.188787548985397E-5 3.891590661739048E-5 16-17 0.0 5.188787548985397E-5 0.0 5.188787548985397E-5 3.891590661739048E-5 18-19 0.0 5.188787548985397E-5 0.0 7.134582879854922E-5 6.485984436231747E-5 20-21 0.0 5.188787548985397E-5 0.0 1.232337042884032E-4 7.783181323478096E-5 22-23 0.0 5.188787548985397E-5 0.0 1.7512157977825718E-4 1.1026173541593969E-4 24-25 0.0 5.188787548985397E-5 0.0 2.8538331519419686E-4 1.4269165759709843E-4 26-27 0.0 5.188787548985397E-5 0.0 4.6050489497245404E-4 1.4269165759709843E-4 28-29 0.0 5.188787548985397E-5 0.0 0.0012647669650651906 1.4269165759709843E-4 30-31 2.5943937744926987E-5 5.188787548985397E-5 0.0 0.004546675089798455 1.4269165759709843E-4 32-33 2.5943937744926987E-5 5.188787548985397E-5 0.0 0.012219594677860612 1.4917764203333016E-4 34-35 2.5943937744926987E-5 7.783181323478096E-5 0.0 0.024315955651432818 1.6863559534202542E-4 36-37 2.5943937744926987E-5 1.0377575097970796E-4 0.0 0.04348203966049763 1.6863559534202542E-4 38-39 2.5943937744926987E-5 1.362056731608667E-4 0.0 0.07370024114890134 1.6863559534202542E-4 40-41 2.5943937744926987E-5 1.4269165759709843E-4 0.0 0.10875050104229769 1.6863559534202542E-4 42-43 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.14205603112234771 1.6863559534202542E-4 44-45 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.1852851173898323 1.7512157977825718E-4 46-47 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.24029923737795 1.816075642144889E-4 48-49 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.30010001388000673 2.075515019594159E-4 50-51 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.3641101942811778 2.075515019594159E-4 52-53 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.4290284125034214 2.2052347083187937E-4 54-55 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.503772897146556 2.3349543970434288E-4 56-57 2.5943937744926987E-5 1.6863559534202542E-4 0.0 0.5946674830359077 2.3349543970434288E-4 58-59 2.5943937744926987E-5 1.7512157977825718E-4 0.0 0.6948240547001984 2.3998142414057464E-4 60-61 2.5943937744926987E-5 2.075515019594159E-4 0.0 0.8042620700927365 2.464674085768064E-4 62-63 2.5943937744926987E-5 2.075515019594159E-4 0.0 0.919329919975924 2.464674085768064E-4 64-65 2.5943937744926987E-5 2.075515019594159E-4 0.0 1.039424407797191 2.464674085768064E-4 66-67 3.242992218115873E-5 2.075515019594159E-4 0.0 1.1803583636120707 2.464674085768064E-4 68-69 3.891590661739048E-5 2.075515019594159E-4 0.0 1.3427479559420048 2.464674085768064E-4 70-71 3.891590661739048E-5 2.075515019594159E-4 0.0 1.520528789339117 2.7241134632173335E-4 72-73 3.891590661739048E-5 2.075515019594159E-4 0.0 1.7034659803630334 3.5024315955651435E-4 74-75 3.891590661739048E-5 2.1403748639564765E-4 0.0 1.8897758832937903 3.5024315955651435E-4 76-77 3.891590661739048E-5 2.205234708318794E-4 0.0 2.097573852661783 3.567291439927461E-4 78-79 3.891590661739048E-5 2.205234708318794E-4 0.0 2.338145501386055 3.632151284289778E-4 80-81 3.891590661739048E-5 2.3349543970434288E-4 0.0 2.596157962259354 3.8267308173767304E-4 82-83 3.891590661739048E-5 2.529533930130381E-4 0.0 2.866454877654876 4.410469416637588E-4 84-85 3.891590661739048E-5 2.594393774492699E-4 0.0 3.1437177403349104 4.410469416637588E-4 86-87 3.891590661739048E-5 2.594393774492699E-4 0.0 3.434244441187039 4.410469416637588E-4 88 3.891590661739048E-5 2.594393774492699E-4 0.0 3.666462141957444 4.5401891053622226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14475 0.0 45.93944 1 GTATCAA 23560 0.0 35.84973 1 TCAACGC 28555 0.0 29.07995 4 GTACCTG 7380 0.0 28.99412 1 ATCAACG 28760 0.0 28.839989 3 CAACGCA 28850 0.0 28.831467 5 TACCTGG 7210 0.0 28.829443 2 AACGCAG 29840 0.0 27.90588 6 TATCAAC 30310 0.0 27.524338 2 GTACATG 25980 0.0 24.88963 1 ACGCAGA 33765 0.0 24.633816 7 CGCAGAG 34345 0.0 24.080992 8 TACATGG 26675 0.0 23.694311 2 ACCTGGG 8705 0.0 23.213411 3 ACATGGG 26425 0.0 23.030005 3 CATGGGG 14670 0.0 22.19946 4 GCAGAGT 38285 0.0 21.185295 9 GAGTACT 23105 0.0 20.735384 12-13 AGAGTAC 35045 0.0 19.134764 10-11 CAGAGTA 37260 0.0 18.892706 10-11 >>END_MODULE