FastQCFastQC Report
Mon 6 Jun 2016
ERR1211494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408933
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311600.29935825314660014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281120.2700757128516439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169760.16309068374251232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA169350.048.0708771
GTATCAA281650.037.128811
TCAACGC334650.030.331744
CAACGCA339450.029.9139485
ATCAACG339800.029.8872993
AACGCAG352800.028.853876
TATCAAC358300.028.6634452
TACCTGG85500.027.9396022
GTACATG328000.026.5277461
GTACCTG97100.025.360321
TACATGG332850.025.359372
ACGCAGA399000.025.208367
CGCAGAG404700.024.5754248
ACATGGG337050.024.3308013
CATGGGG195250.021.9501884
GCAGAGT457000.021.1171469
GAGTACT270450.020.45698212-13
ACCTGGG113050.020.327953
CAGAGTA439600.019.25931210-11
AGAGTAC410400.019.02114510-11
Produced by FastQC (version 0.11.5)