FastQCFastQC Report
Mon 6 Jun 2016
ERR1211494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408933
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341940.3285062935845586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314850.30248057125547834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205710.19762832559302668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182200.047.882611
GTATCAA304500.035.7403141
TCAACGC372250.028.5689324
ATCAACG374350.028.3837013
CAACGCA378150.028.048365
TATCAAC390950.027.3552762
AACGCAG392800.027.0489466
TACCTGG87350.024.9819852
GTACCTG94700.024.0368581
ACGCAGA445600.023.7067687
GTACATG332150.023.5189381
CGCAGAG451950.023.2281198
TACATGG334000.022.9516012
ACATGGG334950.022.0415693
GCAGAGT507950.020.1489269
GAGTACT305100.020.07669312-13
CATGGGG190250.019.9586374
AGAGTAC459150.019.25100310-11
ACCTGGG114250.018.9211653
AGTACTT327700.018.64907312-13
Produced by FastQC (version 0.11.5)