##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10408933 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14004394110328 33.0 31.0 34.0 30.0 34.0 2 32.36258298521088 34.0 31.0 34.0 31.0 34.0 3 32.378731902683974 34.0 31.0 34.0 30.0 34.0 4 36.00437162963774 37.0 35.0 37.0 35.0 37.0 5 35.92558103698045 37.0 35.0 37.0 35.0 37.0 6 35.943624192796705 37.0 35.0 37.0 35.0 37.0 7 35.8704236063389 37.0 35.0 37.0 35.0 37.0 8 35.86759882112797 37.0 35.0 37.0 35.0 37.0 9 37.53240461822552 39.0 37.0 39.0 35.0 39.0 10-11 37.49650257139709 39.0 37.0 39.0 35.0 39.0 12-13 37.41172298832167 39.0 37.0 39.0 34.0 39.0 14-15 38.662746604286916 40.0 38.0 41.0 34.0 41.0 16-17 38.59025228618533 40.0 38.0 41.0 34.0 41.0 18-19 38.577288853718244 40.0 38.0 41.0 34.0 41.0 20-21 38.52077893094326 40.0 38.0 41.0 34.0 41.0 22-23 38.417170040387425 40.0 38.0 41.0 34.0 41.0 24-25 38.31161570547144 40.0 38.0 41.0 34.0 41.0 26-27 38.141417857142514 40.0 38.0 41.0 33.0 41.0 28-29 38.17674448476131 40.0 38.0 41.0 33.5 41.0 30-31 38.109405594214124 40.0 37.5 41.0 33.0 41.0 32-33 37.940973200615275 40.0 37.0 41.0 33.0 41.0 34-35 37.83381101598022 40.0 37.0 41.0 33.0 41.0 36-37 37.78260379810303 40.0 37.0 41.0 33.0 41.0 38-39 37.691348335127145 40.0 37.0 41.0 33.0 41.0 40-41 37.53670837347113 40.0 37.0 41.0 32.0 41.0 42-43 37.390144599835544 40.0 36.5 41.0 31.5 41.0 44-45 37.209657704588935 40.0 36.0 41.0 31.0 41.0 46-47 36.962093232803014 39.0 36.0 41.0 30.5 41.0 48-49 36.74712552189547 39.0 36.0 41.0 30.0 41.0 50-51 36.55168911165054 39.0 35.0 41.0 30.0 41.0 52-53 36.35449714202214 39.0 35.0 40.0 29.0 41.0 54-55 36.16201559756413 39.0 35.0 40.0 28.5 41.0 56-57 35.96598930937494 38.5 35.0 40.0 28.0 41.0 58-59 35.710171109757354 38.0 34.0 40.0 28.0 41.0 60-61 35.399721181796444 38.0 34.0 40.0 27.0 41.0 62-63 35.08175838003761 37.5 34.0 40.0 26.0 41.0 64-65 34.76775645496037 37.0 33.5 40.0 26.0 41.0 66-67 34.42715511762829 36.5 33.0 39.0 26.0 41.0 68-69 34.02960092067074 36.0 32.5 39.0 25.5 40.0 70-71 33.643609292133974 35.5 32.0 39.0 25.0 40.0 72-73 33.234265270032964 35.0 32.0 38.0 24.5 40.0 74-75 32.82554878583616 35.0 31.0 37.0 24.0 39.0 76-77 31.282670183389595 33.5 29.5 35.5 21.5 38.5 78-79 32.105099389149686 34.0 31.0 36.0 23.0 39.0 80-81 32.0702501399519 34.5 31.0 36.0 23.0 37.5 82-83 31.78334470017244 34.0 31.0 35.5 23.0 37.0 84-85 31.480643933436788 34.0 31.0 35.0 21.5 37.0 86-87 31.17940234604258 34.0 30.0 35.0 20.5 36.0 88-89 30.898836268808722 34.0 30.0 35.0 20.0 36.0 90-91 30.617804341713025 34.0 30.0 35.0 20.0 35.5 92-93 30.40881606212664 34.0 29.0 35.0 19.0 35.0 94-95 30.19112554572116 34.0 29.0 35.0 19.0 35.0 96-97 29.98802514148184 34.0 29.0 35.0 18.0 35.0 98-99 29.823946604325343 34.0 29.0 35.0 18.0 35.0 100 27.91244222630696 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2995977060889601 1101 2 0.20663477592719914 1101 3 0.2693699030906984 1101 4 0.15718788363857072 1101 5 0.16936858805136978 1101 6 0.17578192513984447 1101 7 0.20788238502694867 1101 8 0.21528928215102638 1101 9 0.2422391542541007 1101 10-11 0.20098553335004965 1101 12-13 0.21808352403451892 1101 14-15 0.2873263030042281 1101 16-17 0.30296555273388037 1101 18-19 0.32160342772215245 1101 20-21 0.30900710047490065 1101 22-23 0.24678728888176948 1101 24-25 0.21798431036684462 1101 26-27 0.19738120544734983 1101 28-29 0.33151911761896713 1101 30-31 0.3837121986582943 1101 32-33 0.4014041453568211 1101 34-35 0.38237554706341825 1101 36-37 0.37909869045171973 1101 38-39 0.3640345328540846 1101 40-41 0.3951367082705346 1101 42-43 0.3977935461666178 1101 44-45 0.3924906814503615 1101 46-47 0.4515294474865499 1101 48-49 0.4648689588399364 1101 50-51 0.46411706706032874 1101 52-53 0.420284175847641 1101 54-55 0.40271870087185135 1101 56-57 0.37392090562972413 1101 58-59 0.3954067970974222 1101 60-61 0.3762008564265855 1101 62-63 0.3348825801191353 1101 64-65 0.3376209284798932 1101 66-67 0.2937585639362794 1101 68-69 0.2819025251939209 1101 70-71 0.25483833836074865 1101 72-73 0.15651468722285955 1101 74-75 0.18794931000253 1101 76-77 0.22866977415157308 1101 78-79 0.2851912129451222 1101 80-81 0.3195363902631101 1101 82-83 0.413229343546206 1101 84-85 0.31585515435200406 1101 86-87 0.22320951918089804 1101 88-89 0.21026080300071825 1101 90-91 0.22029508169726952 1101 92-93 0.13846646407531438 1101 94-95 0.19587046655152207 1101 96-97 0.06878804868189903 1101 98-99 0.04381472795360608 1101 100 0.20607592072517278 1102 1 0.32205044019293894 1102 2 0.23755408667439326 1102 3 0.33426966240698164 1102 4 0.20814798447057115 1102 5 0.1963047552599022 1102 6 0.17062053875001482 1102 7 0.17825581750075514 1102 8 0.148480108216539 1102 9 0.1996477940685466 1102 10-11 0.22419878106075686 1102 12-13 0.27648193801625354 1102 14-15 0.33138215696554596 1102 16-17 0.3421370431094033 1102 18-19 0.32820122035571586 1102 20-21 0.3227092020494524 1102 22-23 0.3140067110114799 1102 24-25 0.27822001627686177 1102 26-27 0.27367961993080314 1102 28-29 0.34438643265517754 1102 30-31 0.34623939921564073 1102 32-33 0.40776912953104016 1102 34-35 0.4027734564551082 1102 36-37 0.424714723943886 1102 38-39 0.4364171780384041 1102 40-41 0.3899009145589858 1102 42-43 0.3976721023079861 1102 44-45 0.4236359467615145 1102 46-47 0.47875025293841844 1102 48-49 0.5062647846474206 1102 50-51 0.5097620392218616 1102 52-53 0.4891457106758139 1102 54-55 0.47325137666127404 1102 56-57 0.48225017142252824 1102 58-59 0.5356114696442802 1102 60-61 0.5726256585326723 1102 62-63 0.5467387260832695 1102 64-65 0.5396992087452617 1102 66-67 0.49374492782025214 1102 68-69 0.5071798078188152 1102 70-71 0.44093741110486206 1102 72-73 0.49048211787039264 1102 74-75 0.500098999794325 1102 76-77 0.5319653062360459 1102 78-79 0.5613321491030092 1102 80-81 0.5156614128406503 1102 82-83 0.4735361418220023 1102 84-85 0.42988439728057415 1102 86-87 0.4315208573336413 1102 88-89 0.479515366171956 1102 90-91 0.4702312016004804 1102 92-93 0.41768936852144023 1102 94-95 0.5105177068596696 1102 96-97 0.5004542637978133 1102 98-99 0.4394747428476151 1102 100 0.5967131054280657 1103 1 0.2938984020314095 1103 2 0.254574113687994 1103 3 0.2956885877514708 1103 4 0.14864637358568444 1103 5 0.1784145416005245 1103 6 0.2045129224806601 1103 7 0.17368788591836193 1103 8 0.1917613663314981 1103 9 0.2003465796497892 1103 10-11 0.22742490439898688 1103 12-13 0.2630540956931213 1103 14-15 0.37711404455556874 1103 16-17 0.37357574877277955 1103 18-19 0.3356793621690244 1103 20-21 0.29661240850170145 1103 22-23 0.35240949785461595 1103 24-25 0.30604828956126084 1103 26-27 0.3439874044824478 1103 28-29 0.38010349750746997 1103 30-31 0.4463721423766174 1103 32-33 0.5035670597167865 1103 34-35 0.47485147514915127 1103 36-37 0.4414417790547063 1103 38-39 0.4242442261835251 1103 40-41 0.4369266444138873 1103 42-43 0.42208956020495947 1103 44-45 0.44739845081461027 1103 46-47 0.5304183636288968 1103 48-49 0.5289551492713329 1103 50-51 0.47869368267887324 1103 52-53 0.47398331809154115 1103 54-55 0.5805028457756549 1103 56-57 0.5547805880383194 1103 58-59 0.5100856401289207 1103 60-61 0.581947058140905 1103 62-63 0.6265990330093913 1103 64-65 0.5940999546546237 1103 66-67 0.559416977028647 1103 68-69 0.5380336991910326 1103 70-71 0.539685797324907 1103 72-73 0.523014248277498 1103 74-75 0.4964849485828964 1103 76-77 0.5346267491509629 1103 78-79 0.5449734253393359 1103 80-81 0.5457104758744791 1103 82-83 0.5085913002856302 1103 84-85 0.5215451795063473 1103 86-87 0.46834397994476973 1103 88-89 0.5032581421050786 1103 90-91 0.5758341938353873 1103 92-93 0.5048216754047488 1103 94-95 0.5587170350464525 1103 96-97 0.5741829526031381 1103 98-99 0.501676737477883 1103 100 0.6192064739724188 1104 1 0.2734726773836371 1104 2 0.22481103161252491 1104 3 0.2649824708800068 1104 4 0.09236750387587733 1104 5 0.14109162332562875 1104 6 0.16135066365582418 1104 7 0.18572383275201076 1104 8 0.1703064113032866 1104 9 0.1835378391635487 1104 10-11 0.2341803227386805 1104 12-13 0.24183864586795067 1104 14-15 0.3680524877491038 1104 16-17 0.2954036758896521 1104 18-19 0.2676250520444867 1104 20-21 0.2390886857792367 1104 22-23 0.2648651274020821 1104 24-25 0.34791479267390457 1104 26-27 0.32434239706481804 1104 28-29 0.39616678765685975 1104 30-31 0.3373021234465625 1104 32-33 0.35455609593846305 1104 34-35 0.47142164910056295 1104 36-37 0.36762954522265545 1104 38-39 0.415202274472513 1104 40-41 0.5516432910038915 1104 42-43 0.5992627126307895 1104 44-45 0.48997949282097153 1104 46-47 0.5269804281999129 1104 48-49 0.4962372934033894 1104 50-51 0.49782554900153286 1104 52-53 0.433028997741161 1104 54-55 0.45597592452642743 1104 56-57 0.5631854335564768 1104 58-59 0.564469937279199 1104 60-61 0.49430786289146056 1104 62-63 0.49755034130419773 1104 64-65 0.6030610019291132 1104 66-67 0.6308761612621083 1104 68-69 0.6130258293047035 1104 70-71 0.5522423002867995 1104 72-73 0.6391419530473357 1104 74-75 0.6811932526106901 1104 76-77 0.652335367231597 1104 78-79 0.676643344298256 1104 80-81 0.7689338439303626 1104 82-83 0.5712272075971967 1104 84-85 0.6500916094593023 1104 86-87 0.7417219646256186 1104 88-89 0.7636256507586126 1104 90-91 0.6120480615145105 1104 92-93 0.6662202487033362 1104 94-95 0.6659302843481889 1104 96-97 0.6369390042468197 1104 98-99 0.7277784862895267 1104 100 0.9342762721966302 1105 1 0.21017090614814293 1105 2 0.15650050484988753 1105 3 0.20741023740608 1105 4 0.11317702338538993 1105 5 0.09739515286656797 1105 6 0.10335775387780188 1105 7 0.14119295470629112 1105 8 0.13526330266964948 1105 9 0.1443346626337032 1105 10-11 0.14941425746387438 1105 12-13 0.22859188730298996 1105 14-15 0.2931789606411712 1105 16-17 0.2813503048241941 1105 18-19 0.28412610819776063 1105 20-21 0.2363895924120527 1105 22-23 0.28751621762045687 1105 24-25 0.2796454110388211 1105 26-27 0.29145931012725157 1105 28-29 0.37005821672028816 1105 30-31 0.4066899235487398 1105 32-33 0.42923977495786403 1105 34-35 0.38690335459385494 1105 36-37 0.39706381396889157 1105 38-39 0.37842935297644686 1105 40-41 0.39428913570601054 1105 42-43 0.386030384110569 1105 44-45 0.4374531174892127 1105 46-47 0.49264896677237857 1105 48-49 0.5297103621699009 1105 50-51 0.5362800582105081 1105 52-53 0.5248781449735134 1105 54-55 0.5229820097452915 1105 56-57 0.48416506109332147 1105 58-59 0.5178062326217088 1105 60-61 0.5406428190298271 1105 62-63 0.5436279791448726 1105 64-65 0.46117115957060406 1105 66-67 0.520700609717899 1105 68-69 0.5685790940198245 1105 70-71 0.579425707055357 1105 72-73 0.5806221741360247 1105 74-75 0.6418231630634139 1105 76-77 0.6137677201718894 1105 78-79 0.693157345491656 1105 80-81 0.6859916568095841 1105 82-83 0.6569254507617472 1105 84-85 0.6421443073226669 1105 86-87 0.6325718537602754 1105 88-89 0.6899639324072844 1105 90-91 0.6758023766528858 1105 92-93 0.6536043695941096 1105 94-95 0.7176080320492026 1105 96-97 0.7365840206463687 1105 98-99 0.7834672014245463 1105 100 0.8741168757025015 1106 1 0.23607024893610884 1106 2 0.16149365990613518 1106 3 0.22447424371999602 1106 4 0.13830003749912834 1106 5 0.14791644039804908 1106 6 0.12025471676894739 1106 7 0.13940124196007986 1106 8 0.11011209274667522 1106 9 0.16168288026881328 1106 10-11 0.15254950356518293 1106 12-13 0.1753269891976501 1106 14-15 0.24971510547628384 1106 16-17 0.2609904641822922 1106 18-19 0.26141104968689177 1106 20-21 0.27267685289991306 1106 22-23 0.29851952560395745 1106 24-25 0.302697860115849 1106 26-27 0.2597561370938877 1106 28-29 0.33732854571605486 1106 30-31 0.3386223169405653 1106 32-33 0.39115730008441574 1106 34-35 0.3861529685000491 1106 36-37 0.41288575834702357 1106 38-39 0.3449743097631739 1106 40-41 0.40205854270490704 1106 42-43 0.3836459199405624 1106 44-45 0.4500653221944404 1106 46-47 0.5604351024727805 1106 48-49 0.5107256000865092 1106 50-51 0.5068977565311812 1106 52-53 0.5709059613877372 1106 54-55 0.4730276985268915 1106 56-57 0.5116852518504587 1106 58-59 0.6345927720763598 1106 60-61 0.5753741336731295 1106 62-63 0.6284298289848351 1106 64-65 0.6878383509756958 1106 66-67 0.6836358787168848 1106 68-69 0.5865654625944643 1106 70-71 0.5937260521038539 1106 72-73 0.6770480812737674 1106 74-75 0.6483040428944591 1106 76-77 0.5794023877414745 1106 78-79 0.6969479614635148 1106 80-81 0.7207135322510965 1106 82-83 0.6996979452931029 1106 84-85 0.6967916983272886 1106 86-87 0.6066039549631341 1106 88-89 0.6852031644094687 1106 90-91 0.7519446723154815 1106 92-93 0.7233901545215922 1106 94-95 0.7841074522995193 1106 96-97 0.8874253732413564 1106 98-99 0.862028353445627 1106 100 1.0204044680848803 1107 1 0.20942911108053153 1107 2 0.1964161488881686 1107 3 0.25458565617723394 1107 4 0.15498216527673492 1107 5 0.16105848742859052 1107 6 0.16942158889888503 1107 7 0.17644923561126546 1107 8 0.1461335277021405 1107 9 0.19204416174321182 1107 10-11 0.19662118199462242 1107 12-13 0.2046845183991266 1107 14-15 0.21746923143554397 1107 16-17 0.23410720070646818 1107 18-19 0.18255512695134968 1107 20-21 0.24437583508624527 1107 22-23 0.3153004298478024 1107 24-25 0.3163947735439052 1107 26-27 0.32461991494700726 1107 28-29 0.35848381430790965 1107 30-31 0.28834201367678247 1107 32-33 0.30496691653586083 1107 34-35 0.34166441794067737 1107 36-37 0.31671648614928216 1107 38-39 0.34562296372193657 1107 40-41 0.38277744583924544 1107 42-43 0.34302113182455685 1107 44-45 0.354737056098962 1107 46-47 0.3712240814230725 1107 48-49 0.37763729454207606 1107 50-51 0.35989084911293645 1107 52-53 0.4042709296436655 1107 54-55 0.4494367848830976 1107 56-57 0.43736387776348096 1107 58-59 0.48466302125193295 1107 60-61 0.501113551030663 1107 62-63 0.5411173655829771 1107 64-65 0.5950586448395256 1107 66-67 0.5537114249923505 1107 68-69 0.466407512706958 1107 70-71 0.6068191154070846 1107 72-73 0.5215306412947243 1107 74-75 0.5446813466513518 1107 76-77 0.4815968124956882 1107 78-79 0.6370317326904456 1107 80-81 0.6056478674746941 1107 82-83 0.5727601698439102 1107 84-85 0.43819631585741803 1107 86-87 0.4210558495912977 1107 88-89 0.4803820715503413 1107 90-91 0.43506063835240383 1107 92-93 0.5071288668832921 1107 94-95 0.6389933431368853 1107 96-97 0.655974988768218 1107 98-99 0.7141838108068654 1107 100 0.9287253452075745 1108 1 0.2287042960074217 1108 2 0.1782471448932128 1108 3 0.18631989736225307 1108 4 0.13629983063059115 1108 5 0.1087489990128816 1108 6 0.09502102386653632 1108 7 0.1098178421629612 1108 8 0.13087138126846298 1108 9 0.08534798628270579 1108 10-11 0.12113559763802328 1108 12-13 0.1561258039406468 1108 14-15 0.17544197265172556 1108 16-17 0.23384509105874685 1108 18-19 0.22929879889493066 1108 20-21 0.20209733593794255 1108 22-23 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2316 56-57 -0.6970909175074027 2316 58-59 -0.6771063395968042 2316 60-61 -0.4205540586686638 2316 62-63 -0.35125629380808476 2316 64-65 -0.4303219863003065 2316 66-67 -0.20900463356215226 2316 68-69 -0.2929103554261516 2316 70-71 -0.46673990592177717 2316 72-73 -0.5204292549864107 2316 74-75 -0.6391292776657167 2316 76-77 -0.5744719179199862 2316 78-79 -0.42404625604376633 2316 80-81 -0.3279251005643289 2316 82-83 -0.33666510361883084 2316 84-85 -0.3396462690345956 2316 86-87 -0.38401910644624593 2316 88-89 -0.29712257567981126 2316 90-91 -0.21466622217608133 2316 92-93 -0.15196415522382978 2316 94-95 0.002980439719358685 2316 96-97 -0.0581276048861703 2316 98-99 -0.2438058269441079 2316 100 -0.4292609634841611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 13.0 11 49.0 12 92.0 13 264.0 14 851.0 15 2334.0 16 5318.0 17 10841.0 18 19343.0 19 30037.0 20 43168.0 21 60565.0 22 79535.0 23 93649.0 24 101042.0 25 111125.0 26 129420.0 27 155510.0 28 188246.0 29 227011.0 30 273962.0 31 331584.0 32 407454.0 33 512328.0 34 674079.0 35 927124.0 36 1370051.0 37 2142294.0 38 2167716.0 39 343874.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.800566628739624 20.228995715937785 14.041590115230907 23.928847540091688 2 14.980848201349902 21.413808638142815 39.95885646428244 23.646486696224844 3 17.24354126412452 26.67928285627766 31.073371424003888 25.00380445559393 4 12.00156251606205 17.824826616342836 38.49192605563745 31.681684811957666 5 12.470166984187372 38.28128587719778 35.82885393759301 13.419693201021833 6 30.54327329350319 38.31616463961307 17.90006367647279 13.240498390410945 7 26.395443221701974 33.05193721585104 22.986381024837033 17.566238537609955 8 25.246175744733506 36.586102330099926 20.491690511819588 17.676031413346983 9 26.04577992166342 15.36670916222897 20.544071292630463 38.04343962347715 10-11 24.25861036861319 26.629588258469912 29.29412649692336 19.817674875993532 12-13 24.862706869186304 24.460571511028075 29.103155914251726 21.57356570553389 14-15 22.201449466530335 25.903831833675937 26.658505727724446 25.23621297206928 16-17 21.81125275384477 28.770912539720385 27.36790059503654 22.049934111398308 18-19 21.6369576346127 27.90211816128358 29.225289851664677 21.235634352439046 20-21 22.46068594312905 27.10805603587335 29.219666818399908 21.211591202597692 22-23 22.22641840426872 27.035475201925113 28.965139846706673 21.772966547099497 24-25 21.971949370196942 27.16802214560317 29.11811239744802 21.74191608675187 26-27 21.997709559655853 27.282366736477016 29.029220191094833 21.6907035127723 28-29 21.97510333181299 27.250455082695474 29.156722578370413 21.61771900712112 30-31 22.190250180929556 27.198792515564072 29.11146749940219 21.499489804104183 32-33 21.973361066710222 27.330027585262723 29.018072730460638 21.67853861756642 34-35 22.160942653545327 27.39138914482312 29.025872148601767 21.421796053029787 36-37 22.22689081483412 27.310334875794272 28.90247333518145 21.560300974190156 38-39 22.08276482366737 27.426136015373153 28.926343436136793 21.564755724822685 40-41 22.19272149977211 27.290917112396055 28.930505030164078 21.585856357667762 42-43 22.034497650660605 27.337221596916677 28.837541775774877 21.79073897664784 44-45 22.088972584041066 27.524491376371323 28.70894719399224 21.677588845595366 46-47 22.07947323307533 27.443738962177733 28.5222827879797 21.954505016767236 48-49 22.05353046177346 27.514152165012103 28.537644924669507 21.89467244854493 50-51 22.103760730675294 27.71197600938618 28.315762273132183 21.868500986806342 52-53 22.13367668035706 27.773317755065396 28.182031119322765 21.910974445254787 54-55 22.072041432354947 27.719680416637004 28.250867041027405 21.957411109980644 56-57 22.11896817948795 27.763264183035773 28.145278841638365 21.972488795837915 58-59 22.075895587123796 27.686074436980395 28.30187510033806 21.93615487555775 60-61 22.19167455632331 27.746628552915592 28.26042342619845 21.801273464562648 62-63 22.028650198938895 27.779804634499445 28.287632331671098 21.903912834890566 64-65 22.008376710939295 27.629815649237848 28.30058950374264 22.061218136080214 66-67 21.979131817344168 27.69215159789168 28.15984431828816 22.168872266475994 68-69 21.95850286234618 27.689387794064807 28.230091884000334 22.122017459588676 70-71 21.92665462700078 27.538713303222316 28.21704218324997 22.317589886526935 72-73 21.882483619325587 27.719092991462745 28.060724342051596 22.33769904716007 74-75 21.85508366407284 27.789093452786894 27.99600850045484 22.35981438268543 76-77 21.828752086673617 27.663166215782 28.09211872304005 22.415962974504332 78-79 21.774241742953553 27.629332211128276 28.08318633215732 22.513239713760846 80-81 21.76108457742178 27.818900312458673 27.86562763898065 22.5543874711389 82-83 21.798754459289608 27.773791757961575 27.96710491104866 22.46034887170016 84-85 21.754726657978658 27.757121666176214 27.926708425168005 22.561443250677122 86-87 21.639513780096742 27.718468540105494 28.017078062342748 22.624939617455016 88-89 21.600437794251114 27.614914985180246 28.015204605872267 22.769442614696374 90-91 21.694737609345555 27.664563009605192 27.948513230053635 22.692186150995617 92-93 21.588313124573688 27.60290802397517 27.964813284707695 22.843965566743446 94-95 21.41979130406034 27.58550845872665 27.981337615379694 23.013362621833323 96-97 21.428797247279558 27.50131215083383 28.045559260690688 23.024331341195925 98-99 21.35144245376551 27.433050723845298 27.96061576672293 23.25489105566626 100 20.511268300454443 27.886307480881584 28.212924746098338 23.389499472565632 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3189.0 1 2375.5 2 2299.0 3 3197.0 4 2629.5 5 2258.5 6 3534.0 7 7039.5 8 11270.5 9 13408.5 10 11787.5 11 9383.0 12 9389.5 13 9998.0 14 10716.5 15 11423.0 16 12204.5 17 13058.0 18 13959.0 19 15227.5 20 17235.5 21 20252.0 22 24182.0 23 30079.0 24 38438.5 25 48536.5 26 60740.5 27 75973.5 28 93262.0 29 111951.0 30 133158.0 31 153920.5 32 175588.0 33 199036.0 34 222050.0 35 244175.5 36 270084.0 37 295029.5 38 313357.0 39 329148.0 40 340773.5 41 350343.5 42 360709.0 43 371972.0 44 381554.5 45 390874.0 46 397986.0 47 401259.0 48 406153.0 49 406256.0 50 398960.0 51 383864.0 52 362132.5 53 342488.0 54 323072.0 55 297604.0 56 267226.0 57 234645.5 58 202458.5 59 171672.0 60 141332.0 61 113939.5 62 90153.5 63 68614.5 64 49903.5 65 35707.0 66 25579.5 67 18963.5 68 14047.0 69 10155.5 70 7472.0 71 5489.0 72 3937.5 73 2789.0 74 1943.0 75 1291.5 76 853.0 77 553.0 78 362.5 79 227.0 80 155.5 81 124.0 82 101.0 83 70.5 84 50.5 85 33.0 86 28.5 87 25.0 88 11.5 89 10.0 90 8.5 91 7.0 92 5.5 93 4.0 94 3.5 95 3.5 96 2.5 97 2.5 98 5.0 99 6.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034009249555165743 2 0.07012246115908326 3 8.16606274629686E-4 4 7.685706114161751E-4 5 0.0015659626207604562 6 6.244636217756422E-4 7 0.0 8 0.0010471774580545384 9 2.882139792810656E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.349456471667173E-4 18-19 0.001128838085517507 20-21 0.003026246782451189 22-23 0.0 24-25 4.323209689215984E-5 26-27 1.6812482124728826E-4 28-29 0.0010615881570185917 30-31 0.0017869266715426066 32-33 0.0028389076959184963 34-35 0.004255959760717069 36-37 0.0012825522078007418 38-39 0.001335391437335604 40-41 0.0050437446374186476 42-43 0.006609707258179104 44-45 0.005038941071097297 46-47 0.0065952965592150515 48-49 0.00861279441418251 50-51 0.008286151904330637 52-53 0.00399656717936411 54-55 0.00245942595653176 56-57 0.002891746925453358 58-59 0.003554639077799809 60-61 0.00759924192037743 62-63 0.014084056454201407 64-65 0.004155084867968696 66-67 0.009904953754625955 68-69 0.012484468869191492 70-71 0.0053799942799132245 72-73 0.007531991991878513 74-75 0.0030118360834871355 76-77 0.0030886931446287525 78-79 0.008194884144224965 80-81 0.006499225232788028 82-83 0.016581910941303974 84-85 0.01797014160817444 86-87 0.022730475832633372 88-89 0.02130862020151345 90-91 0.024320456285000583 92-93 0.034446374090408696 94-95 0.037842495479603916 96-97 0.02262479737356365 98-99 0.018013373705066598 100 0.018743515785911966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0408933E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.8287683668749 #Duplication Level Percentage of deduplicated Percentage of total 1 70.96651739275214 41.748728135020144 2 17.429769600129198 20.507437569879965 3 5.813536713905144 10.260096142039462 4 2.387434759472975 5.617993858242453 5 1.1306508384887455 3.3257398140633643 6 0.6633541728174714 2.3414585386727356 7 0.3869591622393304 1.593503164597227 8 0.2620334326468714 1.2332083290847928 9 0.17522208074073484 0.9277289280592685 >10 0.7357647011606728 7.198398757000322 >50 0.031010562156813082 1.2465637862106032 >100 0.016062044078927 1.7244271077973181 >500 0.0010615574649134659 0.4205619423816642 >1k 5.07614922007E-4 0.5390970465455717 >5k 4.61468096690286E-5 0.14431664037144828 >10k+ 6.92202145035429E-5 1.1707402400336169 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34194 0.3285062935845586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31485 0.30248057125547834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20571 0.19762832559302668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.607132642702187E-6 0.0 0.0 0.0 0.0 6 9.607132642702187E-6 0.0 0.0 0.0 0.0 7 9.607132642702187E-6 0.0 0.0 0.0 0.0 8 9.607132642702187E-6 0.0 0.0 0.0 0.0 9 9.607132642702187E-6 0.0 0.0 9.607132642702187E-6 9.607132642702187E-6 10-11 9.607132642702187E-6 0.0 0.0 1.4410698964053282E-5 9.607132642702187E-6 12-13 9.607132642702187E-6 1.9214265285404375E-5 0.0 2.4017831606755467E-5 9.607132642702187E-6 14-15 9.607132642702187E-6 3.842853057080875E-5 0.0 4.3232096892159845E-5 3.362496424945765E-5 16-17 9.607132642702187E-6 3.842853057080875E-5 0.0 5.2839229534862024E-5 5.764279585621312E-5 18-19 1.4410698964053282E-5 3.842853057080875E-5 0.0 6.72499284989153E-5 5.764279585621312E-5 20-21 1.9214265285404375E-5 3.842853057080875E-5 0.0 7.68570611416175E-5 6.244636217756422E-5 22-23 1.9214265285404375E-5 3.842853057080875E-5 0.0 1.2489272435512843E-4 7.20534948202664E-5 24-25 1.9214265285404375E-5 4.3232096892159845E-5 0.0 2.3537474974620357E-4 8.646419378431968E-5 26-27 2.4017831606755467E-5 4.8035663213510935E-5 0.0 4.179102699575451E-4 8.646419378431968E-5 28-29 2.882139792810656E-5 4.8035663213510935E-5 0.0 0.0011192309528748048 8.646419378431968E-5 30-31 2.882139792810656E-5 4.8035663213510935E-5 0.0 0.003862067322366279 8.646419378431968E-5 32-33 3.842853057080875E-5 4.8035663213510935E-5 0.0 0.010673524366042129 8.646419378431968E-5 34-35 3.842853057080875E-5 4.8035663213510935E-5 0.0 0.021327834466798853 8.646419378431968E-5 36-37 3.842853057080875E-5 4.8035663213510935E-5 0.0 0.03945169019725653 8.646419378431968E-5 38-39 3.842853057080875E-5 5.2839229534862024E-5 0.0 0.0665438042496767 8.646419378431968E-5 40-41 3.842853057080875E-5 7.68570611416175E-5 0.0 0.09768052114467449 9.607132642702187E-5 42-43 3.842853057080875E-5 9.607132642702187E-5 0.0 0.12929279110548603 9.607132642702187E-5 44-45 3.842853057080875E-5 9.607132642702187E-5 0.0 0.16999821211261518 9.607132642702187E-5 46-47 3.842853057080875E-5 1.0567845906972405E-4 0.0 0.22127147902671676 9.607132642702187E-5 48-49 3.842853057080875E-5 1.2008915803377734E-4 0.0 0.2768487413647489 1.0567845906972405E-4 50-51 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.3370662487692062 1.0567845906972405E-4 52-53 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.39770166644362104 1.1528559171242624E-4 54-55 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.46770884201099194 1.2489272435512843E-4 56-57 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.5513437352320358 1.2489272435512843E-4 58-59 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.6458731168699039 1.344998569978306E-4 60-61 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.7478768477037945 1.441069896405328E-4 62-63 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.8548090375833911 1.441069896405328E-4 64-65 3.842853057080875E-5 1.2489272435512843E-4 0.0 0.9660212050553116 1.441069896405328E-4 66-67 3.842853057080875E-5 1.2489272435512843E-4 0.0 1.0967166375266322 1.441069896405328E-4 68-69 3.842853057080875E-5 1.2489272435512843E-4 0.0 1.2493019217243497 1.441069896405328E-4 70-71 4.8035663213510935E-5 1.2489272435512843E-4 0.0 1.4185411703581914 1.8253552021134156E-4 72-73 4.8035663213510935E-5 1.2489272435512843E-4 0.0 1.5903215055760278 1.9214265285404374E-4 74-75 4.8035663213510935E-5 1.2489272435512843E-4 0.0 1.7657285333664845 1.9214265285404374E-4 76-77 4.8035663213510935E-5 1.2489272435512843E-4 0.0 1.9608878258703366 1.9214265285404374E-4 78-79 4.8035663213510935E-5 1.2489272435512843E-4 0.0 2.1882742448241332 1.9214265285404374E-4 80-81 4.8035663213510935E-5 1.2969629067647952E-4 0.0 2.4346011257830176 2.06553351818097E-4 82-83 4.8035663213510935E-5 1.344998569978306E-4 0.0 2.693018583172742 2.209640507821503E-4 84-85 4.8035663213510935E-5 1.441069896405328E-4 0.0 2.9507203091805856 2.209640507821503E-4 86-87 4.8035663213510935E-5 1.441069896405328E-4 0.0 3.223188198060262 2.3057118342485248E-4 88 4.8035663213510935E-5 1.441069896405328E-4 0.0 3.4455885151724965 2.5939258135295904E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18220 0.0 47.88261 1 GTATCAA 30450 0.0 35.740314 1 TCAACGC 37225 0.0 28.568932 4 ATCAACG 37435 0.0 28.383701 3 CAACGCA 37815 0.0 28.04836 5 TATCAAC 39095 0.0 27.355276 2 AACGCAG 39280 0.0 27.048946 6 TACCTGG 8735 0.0 24.981985 2 GTACCTG 9470 0.0 24.036858 1 ACGCAGA 44560 0.0 23.706768 7 GTACATG 33215 0.0 23.518938 1 CGCAGAG 45195 0.0 23.228119 8 TACATGG 33400 0.0 22.951601 2 ACATGGG 33495 0.0 22.041569 3 GCAGAGT 50795 0.0 20.148926 9 GAGTACT 30510 0.0 20.076693 12-13 CATGGGG 19025 0.0 19.958637 4 AGAGTAC 45915 0.0 19.251003 10-11 ACCTGGG 11425 0.0 18.921165 3 AGTACTT 32770 0.0 18.649073 12-13 >>END_MODULE