FastQCFastQC Report
Mon 6 Jun 2016
ERR1211493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457596
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240670.3726928720842865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207810.32180706256631725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138390.21430575712695557No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73470.11377298920527082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG78400.046.71932
GGTATCA130100.045.730521
GTACCTG86000.043.2379381
ACCTGGG90900.039.4451263
GTATCAA211900.035.1186031
GTACATG253150.029.2287981
TCAACGC251200.029.1075614
CAACGCA253600.028.868265
ATCAACG253000.028.864443
TACATGG258600.028.1278632
CATGGGG156800.027.9609174
ATGGGGG106100.027.9457875
AACGCAG262900.027.9301516
TATCAAC265500.027.5562442
CCTGGGG81450.027.5196674
ACATGGG257700.027.097993
TGGGGGG113050.025.1414136
ACGCAGA293750.024.757577
CGCAGAG299900.024.0939668
GCAGAGT328950.021.2394439
Produced by FastQC (version 0.11.5)