FastQCFastQC Report
Mon 6 Jun 2016
ERR1211493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6457596
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266390.4125219354075418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233440.361496755139219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163320.2529114549748854No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73710.11414464453954691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG79000.048.687752
GTACCTG87500.044.6043241
GGTATCA142350.042.4145161
ACCTGGG93550.040.1372453
GTATCAA227950.034.4289441
TATAACG6600.032.05992
CCTGGGG82500.029.5634174
CAACGCA280950.027.5989635
TCAACGC280500.027.5764394
ATCAACG281750.027.4042683
TGGGGGG99350.026.8663256
TATCAAC289300.026.7856562
GTACATG263650.026.7172051
AACGCAG291100.026.7167456
CATGGGG157050.026.4218944
ATGGGGG103350.025.8271055
TACATGG269600.025.7081682
ACATGGG267350.024.7671133
ACGCAGA325250.023.752487
TAACGCA8500.023.22054
Produced by FastQC (version 0.11.5)