##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6457596 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.128005685087764 33.0 31.0 34.0 30.0 34.0 2 32.349193105298006 34.0 31.0 34.0 31.0 34.0 3 32.360440479707925 34.0 31.0 34.0 30.0 34.0 4 35.99794908198035 37.0 35.0 37.0 35.0 37.0 5 35.92463015648548 37.0 35.0 37.0 35.0 37.0 6 35.94344195579903 37.0 35.0 37.0 35.0 37.0 7 35.87305508117882 37.0 35.0 37.0 35.0 37.0 8 35.865169329267424 37.0 35.0 37.0 35.0 37.0 9 37.518758064146475 39.0 37.0 39.0 35.0 39.0 10-11 37.483264902294906 39.0 37.0 39.0 35.0 39.0 12-13 37.38782071532502 39.0 37.0 39.0 34.0 39.0 14-15 38.61815743815501 40.0 38.0 41.0 34.0 41.0 16-17 38.54264791417735 40.0 38.0 41.0 34.0 41.0 18-19 38.5339583027492 40.0 38.0 41.0 34.0 41.0 20-21 38.47691013807615 40.0 38.0 41.0 34.0 41.0 22-23 38.368564168461454 40.0 38.0 41.0 34.0 41.0 24-25 38.260541848700356 40.0 38.0 41.0 34.0 41.0 26-27 38.07928647131224 40.0 37.0 41.0 33.0 41.0 28-29 38.1126702568572 40.0 38.0 41.0 33.0 41.0 30-31 38.02404098986682 40.0 37.0 41.0 33.0 41.0 32-33 37.82554854778775 40.0 37.0 41.0 33.0 41.0 34-35 37.706134140320955 40.0 37.0 41.0 33.0 41.0 36-37 37.64744287192943 40.0 37.0 41.0 33.0 41.0 38-39 37.552802699332695 40.0 37.0 41.0 32.0 41.0 40-41 37.385189163273765 40.0 36.0 41.0 31.5 41.0 42-43 37.235387673679185 40.0 36.0 41.0 31.0 41.0 44-45 37.05606405231916 39.5 36.0 41.0 31.0 41.0 46-47 36.795824096149715 39.0 35.5 41.0 30.0 41.0 48-49 36.57159398017467 39.0 35.0 41.0 30.0 41.0 50-51 36.370306380269064 39.0 35.0 40.5 29.0 41.0 52-53 36.17311194444496 39.0 35.0 40.0 28.5 41.0 54-55 35.97696650580185 38.5 35.0 40.0 28.0 41.0 56-57 35.779953871378765 38.0 34.5 40.0 28.0 41.0 58-59 35.522138191983515 38.0 34.0 40.0 27.0 41.0 60-61 35.20027716196553 38.0 34.0 40.0 26.5 41.0 62-63 34.86343338914358 37.0 34.0 40.0 26.0 41.0 64-65 34.54847655381353 37.0 33.0 39.5 26.0 41.0 66-67 34.20745297476027 36.0 33.0 39.0 26.0 41.0 68-69 33.8116937789233 36.0 32.5 39.0 25.0 40.0 70-71 33.41907646436847 35.0 32.0 38.5 24.5 40.0 72-73 33.00512102336535 35.0 31.5 37.5 23.5 40.0 74-75 32.595520531169804 35.0 31.0 37.0 22.5 39.0 76-77 31.05506011834745 33.5 29.5 35.5 21.0 38.0 78-79 31.878571452906005 34.0 31.0 36.0 22.0 38.5 80-81 31.85400549987952 34.0 31.0 36.0 22.0 37.5 82-83 31.5680277149577 34.0 31.0 35.0 21.5 37.0 84-85 31.266856659970678 34.0 30.5 35.0 20.0 37.0 86-87 30.975771014476592 34.0 30.0 35.0 20.0 36.0 88-89 30.711385707622462 34.0 30.0 35.0 20.0 36.0 90-91 30.425273817067527 34.0 29.0 35.0 19.0 35.5 92-93 30.20720961794451 34.0 29.0 35.0 18.5 35.0 94-95 29.988913908519514 34.0 29.0 35.0 18.0 35.0 96-97 29.788445111772248 34.0 29.0 35.0 18.0 35.0 98-99 29.62966218388391 34.0 29.0 35.0 17.5 35.0 100 27.732689688236924 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2924614656538793 1101 2 0.21545227012164503 1101 3 0.26882026079272947 1101 4 0.17571414795619944 1101 5 0.1461980941413188 1101 6 0.19970476093003242 1101 7 0.23240205683197956 1101 8 0.15168522326349887 1101 9 0.20571444454150623 1101 10-11 0.20047499160661175 1101 12-13 0.21283363255029997 1101 14-15 0.30370665438452704 1101 16-17 0.2631376400074217 1101 18-19 0.2951530419688666 1101 20-21 0.2550073738496721 1101 22-23 0.2541344357143771 1101 24-25 0.21997994252107134 1101 26-27 0.2623440939522368 1101 28-29 0.302692155213947 1101 30-31 0.27322837700075553 1101 32-33 0.3294916739356779 1101 34-35 0.2837495970843804 1101 36-37 0.3658132315517477 1101 38-39 0.2836566426384195 1101 40-41 0.3780155820440072 1101 42-43 0.30175847377591225 1101 44-45 0.3159276364135195 1101 46-47 0.365859569010631 1101 48-49 0.4000610361931294 1101 50-51 0.2703100724343983 1101 52-53 0.27342031634452724 1101 54-55 0.19763062458236647 1101 56-57 0.14838796489119233 1101 58-59 0.14097516287916534 1101 60-61 0.14674303698806312 1101 62-63 0.08962519594572882 1101 64-65 0.11217425991340946 1101 66-67 0.03345494993339315 1101 68-69 0.08030376655432292 1101 70-71 0.1673208329617779 1101 72-73 0.08620069962716315 1101 74-75 0.14643463731503203 1101 76-77 0.16576652203993092 1101 78-79 0.2116771755739144 1101 80-81 0.16169342892318284 1101 82-83 0.1372544485127314 1101 84-85 0.1450832351846607 1101 86-87 0.06989316958227576 1101 88-89 0.10583890167770704 1101 90-91 0.0687220164928064 1101 92-93 -0.030634179299699582 1101 94-95 -0.028377767499268458 1101 96-97 -0.09708193835578172 1101 98-99 -0.1513441345170321 1101 100 -0.10927338844967238 1102 1 0.3514408832732272 1102 2 0.24007051772658627 1102 3 0.34045991576864765 1102 4 0.2350553119361649 1102 5 0.21055480588248798 1102 6 0.20479290044907827 1102 7 0.17084794119684688 1102 8 0.18437651341879757 1102 9 0.2721082095638323 1102 10-11 0.24009633128942198 1102 12-13 0.24553257255282546 1102 14-15 0.33645016557241547 1102 16-17 0.2923197576013763 1102 18-19 0.3506767589778619 1102 20-21 0.38504897439247543 1102 22-23 0.33519819248564886 1102 24-25 0.3172233090065646 1102 26-27 0.3292750803214233 1102 28-29 0.3570480244134444 1102 30-31 0.40674104520550003 1102 32-33 0.4699260586268039 1102 34-35 0.41520632313990546 1102 36-37 0.44099200415136863 1102 38-39 0.46468051050818104 1102 40-41 0.47692781803142026 1102 42-43 0.4087053106285552 1102 44-45 0.44109603408826104 1102 46-47 0.4375043142622488 1102 48-49 0.4260422941251605 1102 50-51 0.47801856079340865 1102 52-53 0.42103436652159587 1102 54-55 0.4402390054047345 1102 56-57 0.40964885755195724 1102 58-59 0.5026426964002226 1102 60-61 0.44872980142445584 1102 62-63 0.433763879150483 1102 64-65 0.4149414338589281 1102 66-67 0.390899854386511 1102 68-69 0.3968226811528126 1102 70-71 0.34182752173800424 1102 72-73 0.34096326357266804 1102 74-75 0.32683483575303285 1102 76-77 0.46051553988614913 1102 78-79 0.36775486276509284 1102 80-81 0.3682720011680054 1102 82-83 0.29438319584572525 1102 84-85 0.2380693029172356 1102 86-87 0.24006838014359744 1102 88-89 0.2795987344256794 1102 90-91 0.27307088439816596 1102 92-93 0.1505523753211122 1102 94-95 0.21956968707613456 1102 96-97 0.19440362579552684 1102 98-99 0.14553818034868016 1102 100 0.31258899351222524 1103 1 0.25891060725216164 1103 2 0.22805612266283504 1103 3 0.28347791268311084 1103 4 0.14994347564781663 1103 5 0.17909596958758556 1103 6 0.16007203520287305 1103 7 0.15671816737580002 1103 8 0.17346071945191 1103 9 0.17689391660585585 1103 10-11 0.18518779269378172 1103 12-13 0.232047476410294 1103 14-15 0.30661975833308475 1103 16-17 0.29269476883185774 1103 18-19 0.27950351809499097 1103 20-21 0.23820158666930524 1103 22-23 0.27108715862223676 1103 24-25 0.2258733418400496 1103 26-27 0.3047489294046102 1103 28-29 0.2909664608686029 1103 30-31 0.40863516907199227 1103 32-33 0.45418680778553266 1103 34-35 0.40381699820639483 1103 36-37 0.42859808638584695 1103 38-39 0.44920113198070766 1103 40-41 0.4345613624013538 1103 42-43 0.4451473368729779 1103 44-45 0.4371760668176279 1103 46-47 0.5125953823588603 1103 48-49 0.45795933395219635 1103 50-51 0.4971257213466487 1103 52-53 0.4831519855907018 1103 54-55 0.4536678238349836 1103 56-57 0.5003569618895654 1103 58-59 0.6123962852370681 1103 60-61 0.5567946154573704 1103 62-63 0.519871073250556 1103 64-65 0.45507679342126295 1103 66-67 0.4528403692880616 1103 68-69 0.4962099886489142 1103 70-71 0.5547932588487896 1103 72-73 0.5678128607599788 1103 74-75 0.4266375649232401 1103 76-77 0.44217889621256035 1103 78-79 0.5064652143552415 1103 80-81 0.5136677169669106 1103 82-83 0.4827402645307579 1103 84-85 0.4511239391480508 1103 86-87 0.4410922913269957 1103 88-89 0.4427404189165145 1103 90-91 0.43571034959216703 1103 92-93 0.4995770877726571 1103 94-95 0.5669254443497245 1103 96-97 0.5167806004800042 1103 98-99 0.42113950741530815 1103 100 0.481098307001254 1104 1 0.24037359713411632 1104 2 0.23548370714809863 1104 3 0.26856640626760964 1104 4 0.10395914104082493 1104 5 0.1410526665968419 1104 6 0.14474641870599214 1104 7 0.19262661962493866 1104 8 0.19119053143613485 1104 9 0.2535872151572818 1104 10-11 0.22917202856095287 1104 12-13 0.25596805693680125 1104 14-15 0.36056441465675704 1104 16-17 0.28810696981041417 1104 18-19 0.3000702090882825 1104 20-21 0.16371138082462267 1104 22-23 0.23737990789354058 1104 24-25 0.2465533646616933 1104 26-27 0.28451797375034005 1104 28-29 0.35677259794736216 1104 30-31 0.3070060812727746 1104 32-33 0.2679877697822448 1104 34-35 0.39378536960830957 1104 36-37 0.2765724864916024 1104 38-39 0.3367743656823592 1104 40-41 0.42451072240400123 1104 42-43 0.5225183967853582 1104 44-45 0.4387582930106788 1104 46-47 0.42559906001948633 1104 48-49 0.37493587647342963 1104 50-51 0.3558902551134864 1104 52-53 0.4255960791371365 1104 54-55 0.46385022975045587 1104 56-57 0.5813007912671324 1104 58-59 0.5448359434539114 1104 60-61 0.5639882303345942 1104 62-63 0.44358268764284503 1104 64-65 0.5386293451093778 1104 66-67 0.4415704317051734 1104 68-69 0.5335188942253666 1104 70-71 0.5411474537588319 1104 72-73 0.5826727079282321 1104 74-75 0.6243956531289712 1104 76-77 0.5178458379970685 1104 78-79 0.5367397827566656 1104 80-81 0.6602830906792683 1104 82-83 0.5172649257993776 1104 84-85 0.5823260050401693 1104 86-87 0.6540747877008961 1104 88-89 0.6484600232723743 1104 90-91 0.5079688560486773 1104 92-93 0.5020226305650262 1104 94-95 0.5302571731607735 1104 96-97 0.5498065463618182 1104 98-99 0.5826724791538176 1104 100 0.6979557445990707 1105 1 0.1837242584381471 1105 2 0.11702796560272333 1105 3 0.1784842905823254 1105 4 0.09443778517198353 1105 5 0.056044107588448355 1105 6 0.07618623186055373 1105 7 0.10676010764689892 1105 8 0.13198184096950172 1105 9 0.10076373797504345 1105 10-11 0.11735146398132201 1105 12-13 0.17843555048326465 1105 14-15 0.24306213140792465 1105 16-17 0.19234748353378706 1105 18-19 0.19973317210016006 1105 20-21 0.1930133517839323 1105 22-23 0.2582413251027802 1105 24-25 0.21130100852160894 1105 26-27 0.2475698469286982 1105 28-29 0.27919836823272703 1105 30-31 0.26653731174832984 1105 32-33 0.3580449652923434 1105 34-35 0.38155475487340595 1105 36-37 0.3670993048872191 1105 38-39 0.38929836392281203 1105 40-41 0.43242513841549624 1105 42-43 0.38336271629594165 1105 44-45 0.4114381413968644 1105 46-47 0.5061402534855901 1105 48-49 0.5285326003796342 1105 50-51 0.5569991063980453 1105 52-53 0.546473903228943 1105 54-55 0.46521673512510375 1105 56-57 0.4914166858282769 1105 58-59 0.4589673082322534 1105 60-61 0.4723716292108051 1105 62-63 0.5017614627242963 1105 64-65 0.5707733741724113 1105 66-67 0.46304669556847244 1105 68-69 0.5390009771984765 1105 70-71 0.46316547999562374 1105 72-73 0.5045763126676448 1105 74-75 0.4778410273531932 1105 76-77 0.4195691194142164 1105 78-79 0.5640240424100504 1105 80-81 0.6285107511593999 1105 82-83 0.6424508518434457 1105 84-85 0.6137037869718718 1105 86-87 0.686056728955112 1105 88-89 0.6657869205023026 1105 90-91 0.6641876944007556 1105 92-93 0.6302487773451624 1105 94-95 0.5836911793964035 1105 96-97 0.7170961699991061 1105 98-99 0.7558370163121069 1105 100 0.8892755182958432 1106 1 0.22509335734454083 1106 2 0.19471315835692593 1106 3 0.2152253591322122 1106 4 0.10990891557930382 1106 5 0.1273292930312664 1106 6 0.1191709860835175 1106 7 0.15022522037181574 1106 8 0.08316533712518037 1106 9 0.13298472075823753 1106 10-11 0.15303594489021322 1106 12-13 0.17922088545420678 1106 14-15 0.24030279148452394 1106 16-17 0.28154674894190634 1106 18-19 0.2720340521582827 1106 20-21 0.29876359968584154 1106 22-23 0.2922653887240827 1106 24-25 0.3044042225387642 1106 26-27 0.3042618986832011 1106 28-29 0.31276994803683067 1106 30-31 0.33083349626757297 1106 32-33 0.3414944296058948 1106 34-35 0.4122183602328562 1106 36-37 0.3639928159754007 1106 38-39 0.32661782456543165 1106 40-41 0.4104670966704873 1106 42-43 0.4115467440507956 1106 44-45 0.41907103230632003 1106 46-47 0.48050520063792845 1106 48-49 0.5015816999359188 1106 50-51 0.5181580840013211 1106 52-53 0.6043962694393414 1106 54-55 0.4945498497936356 1106 56-57 0.5083809549504892 1106 58-59 0.5509432679667796 1106 60-61 0.6397042655923926 1106 62-63 0.5331225851043087 1106 64-65 0.6006543761784755 1106 66-67 0.5263307193122273 1106 68-69 0.5052660080764184 1106 70-71 0.5773304808663866 1106 72-73 0.5848474942547099 1106 74-75 0.604472856587499 1106 76-77 0.5885092568785559 1106 78-79 0.6238797439508765 1106 80-81 0.7100716928870945 1106 82-83 0.6888660506041724 1106 84-85 0.6285469296724848 1106 86-87 0.5405967738084776 1106 88-89 0.6495913833967322 1106 90-91 0.6417578342666843 1106 92-93 0.709671938923389 1106 94-95 0.7518979333969789 1106 96-97 0.7510487749112436 1106 98-99 0.753790384858597 1106 100 0.9861195657813084 1107 1 0.174734790078233 1107 2 0.14164902125562406 1107 3 0.24099560602370929 1107 4 0.14815774029158035 1107 5 0.12408083083734311 1107 6 0.15385500003802122 1107 7 0.15152580023219286 1107 8 0.1396875009984413 1107 9 0.18858214094726122 1107 10-11 0.14107503201347527 1107 12-13 0.18249562117591722 1107 14-15 0.25071859561099075 1107 16-17 0.24085510979001867 1107 18-19 0.22296612667982885 1107 20-21 0.23992242481092774 1107 22-23 0.2825265906418153 1107 24-25 0.25181718333036684 1107 26-27 0.3216078688189299 1107 28-29 0.3035364023667171 1107 30-31 0.23495523741465973 1107 32-33 0.2625075746582155 1107 34-35 0.3430675415730704 1107 36-37 0.40017192643664146 1107 38-39 0.3753342963452866 1107 40-41 0.36025369768632487 1107 42-43 0.35691739070226447 1107 44-45 0.33164375626439835 1107 46-47 0.3492451403512504 1107 48-49 0.4571557294429027 1107 50-51 0.45091884554575756 1107 52-53 0.4997221323999952 1107 54-55 0.4982520332807141 1107 56-57 0.5363355056904666 1107 58-59 0.6257645214723695 1107 60-61 0.5802580854052408 1107 62-63 0.6384887861783142 1107 64-65 0.7063336164784175 1107 66-67 0.6506227724673153 1107 68-69 0.5276857867076643 1107 70-71 0.7024648057859935 1107 72-73 0.6267554609435777 1107 74-75 0.6702480801599293 1107 76-77 0.6440007380191126 1107 78-79 0.7121582340108326 1107 80-81 0.7419372976365075 1107 82-83 0.7460295621541029 1107 84-85 0.6331941956926386 1107 86-87 0.5548034798427857 1107 88-89 0.5497316188542136 1107 90-91 0.41906669193795665 1107 92-93 0.48625858640273734 1107 94-95 0.6209832311895127 1107 96-97 0.7277574333543377 1107 98-99 0.7582658469528134 1107 100 0.8342905419905264 1108 1 0.19935114025963685 1108 2 0.16642292308756623 1108 3 0.18910234973616014 1108 4 0.13270497404542425 1108 5 0.057211664898098036 1108 6 0.12900638463266745 1108 7 0.1658245882553686 1108 8 0.14180277926309515 1108 9 0.13925702435466292 1108 10-11 0.11697809250605928 1108 12-13 0.1698187668594997 1108 14-15 0.19450836037713515 1108 16-17 0.24044213409734283 1108 18-19 0.20691449689243058 1108 20-21 0.2685023778528901 1108 22-23 0.25895996417551714 1108 24-25 0.2606638817511069 1108 26-27 0.2778987457260911 1108 28-29 0.28222601828717586 1108 30-31 0.30385098911963127 1108 32-33 0.3180095841586308 1108 34-35 0.3213973992915058 1108 36-37 0.3951612781593852 1108 38-39 0.3265809018758645 1108 40-41 0.279009476936551 1108 42-43 0.2960662218928434 1108 44-45 0.2986368049549526 1108 46-47 0.39572029852617874 1108 48-49 0.38701247811368944 1108 50-51 0.3399151551094164 1108 52-53 0.25362366644171175 1108 54-55 0.31074204193330246 1108 56-57 0.39320895133152334 1108 58-59 0.42354741609444346 1108 60-61 0.326923165802377 1108 62-63 0.4421179980066725 1108 64-65 0.5105734305610952 1108 66-67 0.5266744277433517 1108 68-69 0.44184935949162707 1108 70-71 0.5209060930642266 1108 72-73 0.49823222410625334 1108 74-75 0.5653683937052421 1108 76-77 0.5058415410769186 1108 78-79 0.6476845222365277 1108 80-81 0.7485768093442822 1108 82-83 0.7049936496970552 1108 84-85 0.6436960825289724 1108 86-87 0.674314162888134 1108 88-89 0.6518130211244362 1108 90-91 0.6962436347875638 1108 92-93 0.7598746369333291 1108 94-95 0.8483622730604168 1108 96-97 0.8270512148512275 1108 98-99 0.8768590385549757 1108 100 0.6887064248218024 1109 1 0.0603568263474088 1109 2 0.04383181156210725 1109 3 0.1185995972784113 1109 4 0.053849087270620544 1109 5 0.02677152494172219 1109 6 0.072884677868295 1109 7 0.04583077592413787 1109 8 0.06640483943417763 1109 9 0.07127385772200512 1109 10-11 0.04420956425920508 1109 12-13 0.10081940654462329 1109 14-15 0.07057459743640493 1109 16-17 0.15817774530911777 1109 18-19 0.06508363507401782 1109 20-21 0.1225824459450564 1109 22-23 0.1412956259764684 1109 24-25 0.07475687545184684 1109 26-27 0.14317941206816442 1109 28-29 0.21377540931491978 1109 30-31 0.11962174334198039 1109 32-33 0.11747163561000917 1109 34-35 0.12385868618397211 1109 36-37 0.09607809924793287 1109 38-39 0.13867893458933622 1109 40-41 0.07509577445451754 1109 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2316 62-63 -0.481961458227147 2316 64-65 -0.5345637666550473 2316 66-67 -0.14946718281010618 2316 68-69 -0.28536325270320617 2316 70-71 -0.5188790256039084 2316 72-73 -0.4984313646446239 2316 74-75 -0.6413233377516718 2316 76-77 -0.5799600651532479 2316 78-79 -0.48531836280058016 2316 80-81 -0.37728060156520726 2316 82-83 -0.2932451034665853 2316 84-85 -0.2875947574063886 2316 86-87 -0.38182647131904446 2316 88-89 -0.2839678336138185 2316 90-91 -0.1866875691959926 2316 92-93 -0.12416022689582107 2316 94-95 -0.09375157408224055 2316 96-97 -0.15043116880520202 2316 98-99 -0.1817655123417552 2316 100 -0.2993152696585746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 24.0 12 65.0 13 210.0 14 608.0 15 1757.0 16 3906.0 17 8092.0 18 14837.0 19 22667.0 20 31091.0 21 41710.0 22 53741.0 23 62737.0 24 67310.0 25 72574.0 26 83376.0 27 99351.0 28 118920.0 29 144718.0 30 172478.0 31 209719.0 32 258172.0 33 325826.0 34 434940.0 35 584765.0 36 847616.0 37 1313208.0 38 1285300.0 39 197843.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.12304168382399 19.76926616096238 13.6515998629169 23.456092292296727 2 15.266210076159197 21.147655122045784 40.56181180386206 23.024322997932963 3 17.42851590114215 27.60434870473422 30.688457163740768 24.278678230382862 4 12.054291170135551 17.419111362806117 37.916680797410656 32.60991666964767 5 12.20469526666896 38.27070675535759 35.02794123158222 14.49665674639124 6 29.88909729259633 37.52751816787538 18.911671171985915 13.671713367542376 7 26.822303532150354 33.26079860059378 22.808007809717424 17.10889005753844 8 26.07212852967885 36.0465955393457 20.52777781219067 17.353498118784774 9 27.313295429645244 15.565253008279397 20.425251196459364 36.696200365615994 10-11 24.68539375953528 26.366816691536606 29.213115840631716 19.7346737082964 12-13 25.115244124903448 24.272143999098116 28.977509587159062 21.635102288839374 14-15 22.58077928690491 25.64273918653319 26.82930304094589 24.94717848561601 16-17 22.10626932329651 28.36048628312109 27.462770351936605 22.070474041645795 18-19 21.729973602096965 27.543358166652354 29.45725840127027 21.269409829980408 20-21 22.634199402189413 26.891154972441296 29.346951205489706 21.127694419879592 22-23 22.36634964466653 26.681964929363804 29.248903152194718 21.702782273774947 24-25 22.004165482337815 26.69866051535746 29.305280889767715 21.99189311253701 26-27 21.872998236570545 26.770670935546747 29.365672551862442 21.990658276020262 28-29 21.93123437728294 27.232321392802078 29.272338622264023 21.56410560765096 30-31 22.65339014055335 26.81397054980536 29.354848325884 21.17779098375729 32-33 22.316191916840847 26.84892110618498 29.345235025670547 21.489651951303628 34-35 22.377054911489044 27.08652268274464 29.206420049541883 21.33000235622443 36-37 22.33130701206269 27.161919097361757 29.09021504402235 21.4165588465532 38-39 22.32568572672085 27.327636555914232 28.940812407505955 21.405865309858964 40-41 22.380006858952207 26.990278918075788 29.099449309300105 21.5302649136719 42-43 22.186610480801097 27.179215566024588 28.744753869360117 21.889420083814194 44-45 21.89346294632511 27.379299114510307 28.894768218386872 21.832469720777706 46-47 21.921660253652444 27.06076737499423 28.664237743337573 22.35333462801575 48-49 21.93978089698491 27.220238517441548 28.72499595016551 22.114984635408035 50-51 22.042584448527116 27.41887649546646 28.690484689796452 21.848054366209965 52-53 22.09079423253421 27.528798729573595 28.32295460706501 22.057452430827183 54-55 21.88359132705026 27.289922333203304 28.677465448085833 22.149020891660605 56-57 22.045139580920416 27.47532031671389 28.19591580994131 22.283624292424378 58-59 21.96584878834342 27.527708911900906 28.545332261487943 21.96111003826773 60-61 22.45511012932049 27.576676408859903 28.399959362788703 21.568254099030902 62-63 22.264492579666637 27.60530195061949 28.450344277076994 21.679861192636878 64-65 22.118783693189357 27.2319252894879 28.420802895800463 22.22848812152228 66-67 21.978241096444556 27.331223150026258 28.34217368807499 22.348362065454193 68-69 21.729451749817827 27.398454191424097 28.548773660078535 22.323320398679535 70-71 21.905475532617793 27.271844548180923 28.514155456000402 22.30852446320088 72-73 21.80112974523702 27.784935682234156 27.9374882252063 22.47644634732252 74-75 21.797566871467673 27.716282070828278 27.98475672857207 22.501394329131983 76-77 21.63265713817592 27.411169438625976 28.25535516893316 22.70081825426495 78-79 21.57510714126975 27.46666513863979 28.313736646735727 22.644491073354736 80-81 21.69032428416397 27.596857627253822 28.000978748989986 22.711839339592217 82-83 21.91191915362147 27.648104084647766 27.97211407691768 22.467862684813085 84-85 21.794809147110822 27.862332563905646 27.739278858823635 22.603579430159897 86-87 21.5624435377003 27.84433106313423 27.884695436014606 22.70852996315086 88-89 21.355096724029643 27.496032916915457 28.13889785951763 23.00997249953727 90-91 21.9169783755677 27.398302405624964 28.087017859297525 22.59770135950981 92-93 21.73084854697624 27.542959072747813 27.97577383647146 22.75041854380449 94-95 21.486120154276794 27.459427362737138 27.90866549829741 23.145786984688662 96-97 21.368403452118496 27.422714600176278 27.96926311286221 23.239618834843018 98-99 21.28045613871509 27.30389128278869 27.884070164178283 23.531582414317946 100 20.21970904125612 28.018130092583416 28.248179184591898 23.513981681568566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3108.0 1 2324.0 2 3430.0 3 5004.0 4 3433.0 5 2472.0 6 3561.0 7 6700.0 8 10611.5 9 12237.0 10 10269.5 11 7918.5 12 7753.0 13 8301.0 14 8822.0 15 9214.0 16 9666.5 17 10162.5 18 10818.5 19 11543.0 20 12633.0 21 14186.5 22 16325.5 23 19563.0 24 23623.5 25 28750.5 26 35635.5 27 43637.0 28 52846.5 29 63832.5 30 76475.5 31 89069.5 32 102945.5 33 118586.0 34 134454.5 35 148932.5 36 165878.0 37 182045.5 38 193053.5 39 202996.5 40 206171.0 41 207618.5 42 211222.5 43 215136.5 44 220567.5 45 226841.5 46 232726.5 47 236945.5 48 241396.5 49 246621.0 50 252184.0 51 248099.5 52 234953.5 53 222122.5 54 208729.5 55 192147.5 56 173214.0 57 155506.5 58 138047.5 59 116046.0 60 91931.0 61 72352.0 62 57873.5 63 45229.5 64 33570.5 65 24884.5 66 18465.0 67 14001.0 68 10687.0 69 7946.0 70 6006.0 71 4510.5 72 3481.0 73 2710.5 74 2011.5 75 1400.0 76 958.5 77 667.0 78 430.5 79 264.5 80 178.0 81 119.5 82 89.0 83 66.5 84 42.5 85 35.0 86 29.5 87 16.5 88 14.5 89 14.0 90 12.5 91 8.5 92 5.0 93 2.0 94 3.5 95 4.5 96 3.0 97 2.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003081642146705988 2 0.068183268200736 3 6.349111960550025E-4 4 6.968537517676857E-4 5 0.0017653628378114705 6 6.813681128395149E-4 7 0.0 8 0.0010530234471156138 9 1.5485638928170793E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.349111960550025E-4 18-19 0.001169165739076895 20-21 0.0030893849661700733 22-23 0.0 24-25 7.742819464085396E-6 26-27 1.3162793088945172E-4 28-29 8.129960437289667E-4 30-31 0.001293050850502261 32-33 0.0030196995909933045 34-35 0.0035849254118715383 36-37 0.0012543367531818342 38-39 0.0013240221283586028 40-41 0.005288345693970326 42-43 0.007061451351245882 44-45 0.00497863291540691 46-47 0.006411054516262708 48-49 0.008393216299068569 50-51 0.007696362547300883 52-53 0.003662353606512392 54-55 0.0021525038110157403 56-57 0.002462216589579156 58-59 0.0030506708688496464 60-61 0.007123393906958564 62-63 0.013596390978933955 64-65 0.0037939815374018445 66-67 0.010112122220095528 68-69 0.01209428400290139 70-71 0.004885719081837885 72-73 0.007378906949273383 74-75 0.0029345285768883653 76-77 0.0029422713963524506 78-79 0.0072317933794557605 80-81 0.005783886139671791 82-83 0.015563067122811646 84-85 0.01649994827796598 86-87 0.02179603679140039 88-89 0.020766241802677034 90-91 0.023522685531891435 92-93 0.03373546440502007 94-95 0.037862387179377587 96-97 0.02236900543174271 98-99 0.017638142739186534 100 0.01777751348954007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6457596.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.02609041069803 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11528172132644 43.7473531969737 2 16.197025540467287 19.120941878720142 3 4.772049006566464 8.450261883176214 4 1.884570221455722 4.449552491078187 5 0.9202666652157684 2.715987169148876 6 0.5240754205161677 1.8560473892047145 7 0.3385690646798538 1.3989085755440964 8 0.2321515636866867 1.0962399349724232 9 0.16451889379832427 0.8739816389647125 >10 0.7726465750705378 7.928777839057257 >50 0.04805027301917277 1.9651124517159753 >100 0.02789718374345646 3.164181061875308 >500 0.0021203937134449597 0.8779869799081383 >1k 6.361173332285019E-4 0.8175972310776867 >5k 3.533985184584629E-5 0.15375709121722164 >10k+ 1.0601955553753888E-4 1.383313187365358 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26639 0.4125219354075418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23344 0.361496755139219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16332 0.2529114549748854 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7371 0.11414464453954691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0971277856341585E-5 2 0.0 0.0 0.0 0.0 3.0971277856341585E-5 3 0.0 0.0 0.0 0.0 3.0971277856341585E-5 4 0.0 0.0 0.0 1.5485638928170793E-5 3.0971277856341585E-5 5 0.0 0.0 0.0 1.5485638928170793E-5 3.0971277856341585E-5 6 0.0 0.0 0.0 1.5485638928170793E-5 4.645691678451238E-5 7 0.0 0.0 0.0 1.5485638928170793E-5 6.194255571268317E-5 8 0.0 0.0 0.0 1.5485638928170793E-5 7.742819464085396E-5 9 0.0 0.0 0.0 4.645691678451238E-5 1.5485638928170793E-4 10-11 0.0 0.0 0.0 6.968537517676857E-5 1.5485638928170793E-4 12-13 0.0 1.5485638928170793E-5 0.0 1.0839947249719555E-4 1.5485638928170793E-4 14-15 0.0 1.5485638928170793E-5 0.0 1.0839947249719555E-4 1.7034202820987872E-4 16-17 0.0 1.5485638928170793E-5 0.0 1.2388511142536634E-4 1.7034202820987872E-4 18-19 0.0 1.5485638928170793E-5 0.0 1.2388511142536634E-4 2.013133060662203E-4 20-21 0.0 1.5485638928170793E-5 0.0 1.3162793088945172E-4 2.167989449943911E-4 22-23 7.742819464085396E-6 1.5485638928170793E-5 0.0 1.3937075035353713E-4 2.477702228507327E-4 24-25 1.5485638928170793E-5 1.5485638928170793E-5 0.0 2.013133060662203E-4 3.0971277856341585E-4 26-27 1.5485638928170793E-5 1.5485638928170793E-5 0.0 3.948837926683552E-4 3.3294123695567205E-4 28-29 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.0012465939337177488 3.4842687588384287E-4 30-31 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.004808290887197031 3.6391251481201364E-4 32-33 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.013851904021248775 4.181122510606114E-4 34-35 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.02703018274912212 4.335978899887822E-4 36-37 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.04794353812161678 4.49083528916953E-4 38-39 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.08321208078052575 4.645691678451238E-4 40-41 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.1281514049500774 4.645691678451238E-4 42-43 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.17074465482201118 4.723119873092092E-4 44-45 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.2210962097969585 4.955404457014654E-4 46-47 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.28241933995251484 5.265117235578069E-4 48-49 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.35054840841700224 5.574830014141485E-4 50-51 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.4266603237489617 5.574830014141485E-4 52-53 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.5085174111232725 5.729686403423194E-4 54-55 1.5485638928170793E-5 1.5485638928170793E-5 0.0 0.6078035851112396 5.961970987345756E-4 56-57 3.0971277856341585E-5 1.5485638928170793E-5 0.0 0.7200125247847651 6.039399181986609E-4 58-59 3.0971277856341585E-5 1.5485638928170793E-5 0.0 0.8464605094527438 6.039399181986609E-4 60-61 3.0971277856341585E-5 1.5485638928170793E-5 0.0 0.9808448840714098 6.349111960550025E-4 62-63 3.0971277856341585E-5 1.5485638928170793E-5 0.0 1.1253646093685639 6.503968349831733E-4 64-65 3.0971277856341585E-5 1.5485638928170793E-5 0.0 1.2723000943385123 6.658824739113441E-4 66-67 3.0971277856341585E-5 1.5485638928170793E-5 0.0 1.4370595497147853 6.968537517676857E-4 68-69 3.0971277856341585E-5 1.5485638928170793E-5 0.0 1.6353686418289406 7.200822101599419E-4 70-71 3.0971277856341585E-5 1.5485638928170793E-5 0.0 1.856364194972866 7.43310668552198E-4 72-73 3.0971277856341585E-5 1.5485638928170793E-5 0.0 2.068378077538452 8.671957799775644E-4 74-75 3.0971277856341585E-5 1.5485638928170793E-5 0.0 2.289660115002549 8.671957799775644E-4 76-77 3.0971277856341585E-5 1.5485638928170793E-5 0.0 2.5345964659294262 8.671957799775644E-4 78-79 3.0971277856341585E-5 1.5485638928170793E-5 0.0 2.8182159428988744 8.671957799775644E-4 80-81 3.0971277856341585E-5 1.5485638928170793E-5 0.0 3.1084710161490436 9.059098772979913E-4 82-83 3.0971277856341585E-5 1.5485638928170793E-5 0.0 3.415063438468433 9.7559525247476E-4 84-85 3.0971277856341585E-5 1.5485638928170793E-5 0.0 3.74263115871603 9.7559525247476E-4 86-87 3.0971277856341585E-5 1.5485638928170793E-5 0.0 4.077151930842375 9.910808914029307E-4 88 3.0971277856341585E-5 1.5485638928170793E-5 0.0 4.347144045555033 0.0010220521692592723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 7900 0.0 48.68775 2 GTACCTG 8750 0.0 44.604324 1 GGTATCA 14235 0.0 42.414516 1 ACCTGGG 9355 0.0 40.137245 3 GTATCAA 22795 0.0 34.428944 1 TATAACG 660 0.0 32.0599 2 CCTGGGG 8250 0.0 29.563417 4 CAACGCA 28095 0.0 27.598963 5 TCAACGC 28050 0.0 27.576439 4 ATCAACG 28175 0.0 27.404268 3 TGGGGGG 9935 0.0 26.866325 6 TATCAAC 28930 0.0 26.785656 2 GTACATG 26365 0.0 26.717205 1 AACGCAG 29110 0.0 26.716745 6 CATGGGG 15705 0.0 26.421894 4 ATGGGGG 10335 0.0 25.827105 5 TACATGG 26960 0.0 25.708168 2 ACATGGG 26735 0.0 24.767113 3 ACGCAGA 32525 0.0 23.75248 7 TAACGCA 850 0.0 23.2205 4 >>END_MODULE