FastQCFastQC Report
Mon 6 Jun 2016
ERR1211492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8862948
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274210.3093891558429543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245620.2771312660302193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156240.17628445975312051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140350.048.6820261
GTATCAA238050.036.5550581
TCAACGC282300.030.0111964
CAACGCA285150.029.692255
ATCAACG285700.029.605543
AACGCAG295400.028.6564676
TATCAAC303200.028.1155512
GTACATG284650.026.5507951
TACATGG290400.025.1126862
ACGCAGA334650.025.086447
TACCTGG69700.024.8252142
ACATGGG287750.024.6588213
CGCAGAG342250.024.4610148
GTACCTG76650.023.6516931
CATGGGG156050.022.3127384
GCAGAGT385350.021.2636629
GAGTACT239050.020.42211512-13
ACCTGGG88050.019.3192353
CAGAGTA374150.019.27583910-11
AGAGTAC353100.019.12761310-11
Produced by FastQC (version 0.11.5)