FastQCFastQC Report
Mon 6 Jun 2016
ERR1211492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8862948
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304730.3438246506692807No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279340.31517729766664543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186410.21032505211584224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146750.049.86011
GTATCAA251350.036.5006071
TCAACGC304800.029.6164254
ATCAACG305950.029.428643
CAACGCA308800.029.2784445
AACGCAG320250.028.3338786
TATCAAC326550.027.6913382
GTACATG286700.025.6197571
TACCTGG69750.025.3488332
ACGCAGA367800.024.5558247
GTACCTG77100.024.3354951
TACATGG293550.024.284632
CGCAGAG374300.024.003858
ACATGGG288700.023.3251553
GCAGAGT418000.021.2355569
GAGTACT260000.020.78474612-13
CATGGGG158000.020.4026814
TATAACG8250.019.3793542
AGAGTAC388400.019.3273410-11
ACCTGGG92300.018.9394073
Produced by FastQC (version 0.11.5)