##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8862948 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12210327760018 33.0 31.0 34.0 30.0 34.0 2 32.347055291309395 34.0 31.0 34.0 31.0 34.0 3 32.362740478675946 34.0 31.0 34.0 30.0 34.0 4 35.9945487663924 37.0 35.0 37.0 35.0 37.0 5 35.91783952698357 37.0 35.0 37.0 35.0 37.0 6 35.934506103386816 37.0 35.0 37.0 35.0 37.0 7 35.85980240434672 37.0 35.0 37.0 35.0 37.0 8 35.8561627575836 37.0 35.0 37.0 35.0 37.0 9 37.514092263657645 39.0 37.0 39.0 35.0 39.0 10-11 37.48041063763434 39.0 37.0 39.0 34.5 39.0 12-13 37.39544703410198 39.0 37.0 39.0 34.0 39.0 14-15 38.637990147296364 40.0 38.0 41.0 34.0 41.0 16-17 38.567251720307965 40.0 38.0 41.0 34.0 41.0 18-19 38.56082801117641 40.0 38.0 41.0 34.0 41.0 20-21 38.50452073057407 40.0 38.0 41.0 34.0 41.0 22-23 38.39809739377914 40.0 38.0 41.0 34.0 41.0 24-25 38.293322379867284 40.0 38.0 41.0 34.0 41.0 26-27 38.1196870386693 40.0 38.0 41.0 33.0 41.0 28-29 38.159354821894475 40.0 38.0 41.0 33.5 41.0 30-31 38.08779550551351 40.0 37.5 41.0 33.0 41.0 32-33 37.914504067946694 40.0 37.0 41.0 33.0 41.0 34-35 37.807154402801416 40.0 37.0 41.0 33.0 41.0 36-37 37.75020580059818 40.0 37.0 41.0 33.0 41.0 38-39 37.65666316670254 40.0 37.0 41.0 32.5 41.0 40-41 37.500678950164215 40.0 37.0 41.0 32.0 41.0 42-43 37.356355300741924 40.0 36.5 41.0 31.0 41.0 44-45 37.17354440080208 40.0 36.0 41.0 31.0 41.0 46-47 36.92108297374644 39.0 36.0 41.0 30.5 41.0 48-49 36.70579066919946 39.0 36.0 41.0 30.0 41.0 50-51 36.50944894407594 39.0 35.0 41.0 29.5 41.0 52-53 36.31354245788196 39.0 35.0 40.0 29.0 41.0 54-55 36.1245221116044 39.0 35.0 40.0 28.0 41.0 56-57 35.93129729521148 38.5 35.0 40.0 28.0 41.0 58-59 35.679459137072676 38.0 34.0 40.0 28.0 41.0 60-61 35.37227381904982 38.0 34.0 40.0 27.0 41.0 62-63 35.056460107855756 37.5 34.0 40.0 26.0 41.0 64-65 34.7470030852037 37.0 33.5 40.0 26.0 41.0 66-67 34.41255262921547 36.5 33.0 39.0 26.0 41.0 68-69 34.01625266220675 36.0 32.5 39.0 25.5 40.0 70-71 33.62920920894493 35.5 32.0 39.0 25.0 40.0 72-73 33.22025809019753 35.0 32.0 38.0 24.0 40.0 74-75 32.81178926018747 35.0 31.0 37.0 23.5 39.5 76-77 31.25566024984012 33.5 29.5 35.5 21.5 38.5 78-79 32.087345655192834 34.0 31.0 36.0 23.0 39.0 80-81 32.049143806327194 34.5 31.0 36.0 23.0 38.0 82-83 31.758493392943297 34.0 31.0 35.5 22.5 37.0 84-85 31.451091781199665 34.0 31.0 35.0 21.5 37.0 86-87 31.1489296789285 34.0 30.0 35.0 20.0 36.0 88-89 30.86506171535701 34.0 30.0 35.0 20.0 36.0 90-91 30.57312956140553 34.0 29.5 35.0 19.5 36.0 92-93 30.35974113805023 34.0 29.0 35.0 19.0 35.0 94-95 30.136318299509377 34.0 29.0 35.0 18.5 35.0 96-97 29.927468264509734 34.0 29.0 35.0 18.0 35.0 98-99 29.76390857759743 34.0 29.0 35.0 18.0 35.0 100 27.864283193357334 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3077873144958474 1101 2 0.2505225635680901 1101 3 0.3007747873191988 1101 4 0.16475878523688436 1101 5 0.16401939243592523 1101 6 0.1884207985516042 1101 7 0.21007501618915825 1101 8 0.20932162134145216 1101 9 0.24040513530176355 1101 10-11 0.2477050734998656 1101 12-13 0.2507013121525645 1101 14-15 0.33808495265783733 1101 16-17 0.311692335440263 1101 18-19 0.3260046479551306 1101 20-21 0.31848935137454504 1101 22-23 0.28633029141513333 1101 24-25 0.2592766790900498 1101 26-27 0.2289117086865602 1101 28-29 0.3479688906771585 1101 30-31 0.39348080086258364 1101 32-33 0.42275820968299627 1101 34-35 0.43361730487801964 1101 36-37 0.41750587026189834 1101 38-39 0.44532819332903983 1101 40-41 0.47140513628923486 1101 42-43 0.4441278129938766 1101 44-45 0.43174566203673237 1101 46-47 0.4815676127390276 1101 48-49 0.4755579663712197 1101 50-51 0.4788958890551953 1101 52-53 0.4589291271682825 1101 54-55 0.4216579632829607 1101 56-57 0.4348863664817273 1101 58-59 0.43291934279281463 1101 60-61 0.33798039369803945 1101 62-63 0.3551590158549658 1101 64-65 0.4039542807784855 1101 66-67 0.34716893019661654 1101 68-69 0.3669731086831973 1101 70-71 0.37422924367280075 1101 72-73 0.3027780317871489 1101 74-75 0.35573954093776905 1101 76-77 0.3469509528024446 1101 78-79 0.4376704152357931 1101 80-81 0.4215248790970918 1101 82-83 0.3817621900005683 1101 84-85 0.2878446015347329 1101 86-87 0.2997780192692012 1101 88-89 0.2345373059997442 1101 90-91 0.17613563815138633 1101 92-93 0.15300140503395454 1101 94-95 0.21517184038074788 1101 96-97 0.14362838481787676 1101 98-99 0.1199350366312899 1101 100 0.1758795815424321 1102 1 0.34328560131317687 1102 2 0.22178331265134688 1102 3 0.3180272267068318 1102 4 0.20289466366831022 1102 5 0.2170800414373062 1102 6 0.17116751452329737 1102 7 0.18926586530381684 1102 8 0.14957232316243818 1102 9 0.22534936498514213 1102 10-11 0.2448368547603721 1102 12-13 0.25231452556649714 1102 14-15 0.3794137085883946 1102 16-17 0.3490211496510298 1102 18-19 0.34479082911884973 1102 20-21 0.32292787276508506 1102 22-23 0.3075267100501762 1102 24-25 0.348423112774789 1102 26-27 0.3139975272963227 1102 28-29 0.35391745865626234 1102 30-31 0.3984947538237833 1102 32-33 0.4611979727355475 1102 34-35 0.4111400489900845 1102 36-37 0.424559464542817 1102 38-39 0.41585307436190533 1102 40-41 0.4144993460823372 1102 42-43 0.388472598776616 1102 44-45 0.4075226092869286 1102 46-47 0.47390725976811865 1102 48-49 0.42376600788538354 1102 50-51 0.44871583700240336 1102 52-53 0.3932485308954057 1102 54-55 0.4527175175495941 1102 56-57 0.43634654910470516 1102 58-59 0.4645681395106678 1102 60-61 0.4457961414482696 1102 62-63 0.5171517460326811 1102 64-65 0.4634703626062304 1102 66-67 0.4151433443010575 1102 68-69 0.4478517771160142 1102 70-71 0.41557818550997183 1102 72-73 0.46427211225369547 1102 74-75 0.4075012532432254 1102 76-77 0.442300317000182 1102 78-79 0.5121249025472494 1102 80-81 0.44269900525145545 1102 82-83 0.41268310756108306 1102 84-85 0.3222392396189697 1102 86-87 0.2976075541696197 1102 88-89 0.2625023632954431 1102 90-91 0.2409893010372386 1102 92-93 0.1901098434427695 1102 94-95 0.29273533438330546 1102 96-97 0.3297651938422277 1102 98-99 0.2641062457386383 1102 100 0.23004371336534035 1103 1 0.2546641504014744 1103 2 0.19400511623569372 1103 3 0.28395178864668225 1103 4 0.12627266535984916 1103 5 0.14946635336295344 1103 6 0.139453415984093 1103 7 0.14501342130097328 1103 8 0.17496715869636148 1103 9 0.22812699195241493 1103 10-11 0.21605195027306223 1103 12-13 0.231421661171332 1103 14-15 0.3387558255293399 1103 16-17 0.34298373585335895 1103 18-19 0.30291885588327006 1103 20-21 0.29973079022114035 1103 22-23 0.2847918087596426 1103 24-25 0.26189795821721873 1103 26-27 0.27555836442761006 1103 28-29 0.34072759665726693 1103 30-31 0.4348150643316231 1103 32-33 0.45739437531071303 1103 34-35 0.45856479963978103 1103 36-37 0.46212234773391714 1103 38-39 0.45302076844661343 1103 40-41 0.48813973990375814 1103 42-43 0.5071181424491016 1103 44-45 0.4615055945526336 1103 46-47 0.5312334366333147 1103 48-49 0.5316329579507268 1103 50-51 0.5201324567160626 1103 52-53 0.5519334882642468 1103 54-55 0.6033933624934704 1103 56-57 0.5754883003115054 1103 58-59 0.6436579707443215 1103 60-61 0.6399785438046166 1103 62-63 0.6322256731429974 1103 64-65 0.6173062576906219 1103 66-67 0.5989612363429657 1103 68-69 0.613549261804522 1103 70-71 0.6629474903477686 1103 72-73 0.6362596460854419 1103 74-75 0.6126107799058218 1103 76-77 0.6225495632956566 1103 78-79 0.6415174604087 1103 80-81 0.6064629478848715 1103 82-83 0.5812094847026366 1103 84-85 0.43237420322300224 1103 86-87 0.5085448932399252 1103 88-89 0.5581747109486308 1103 90-91 0.6079522785802283 1103 92-93 0.49691353502581137 1103 94-95 0.560966252047006 1103 96-97 0.6030380397531694 1103 98-99 0.5552342086885247 1103 100 0.7571552768878078 1104 1 0.29104428348436073 1104 2 0.21943244274097395 1104 3 0.2793705245958691 1104 4 0.08054447914039997 1104 5 0.15561733909389375 1104 6 0.1966662878665133 1104 7 0.20413611868680448 1104 8 0.22040752957813226 1104 9 0.20483398081615434 1104 10-11 0.2636142427807613 1104 12-13 0.2988522671860423 1104 14-15 0.3809386542467834 1104 16-17 0.2990627494565459 1104 18-19 0.28399554623128154 1104 20-21 0.23678074337797028 1104 22-23 0.27476978295918997 1104 24-25 0.30792827431894665 1104 26-27 0.37865407754011216 1104 28-29 0.42597231042856976 1104 30-31 0.3231628969723701 1104 32-33 0.3047105186290935 1104 34-35 0.4401489322094747 1104 36-37 0.31661227309949425 1104 38-39 0.3999227412256161 1104 40-41 0.4950344721757389 1104 42-43 0.5507562527121337 1104 44-45 0.4818902530438791 1104 46-47 0.43073444898717383 1104 48-49 0.4172995193696707 1104 50-51 0.4186444392246855 1104 52-53 0.40670534460732455 1104 54-55 0.4371272286754291 1104 56-57 0.4838942094530836 1104 58-59 0.5010835973097798 1104 60-61 0.38269253206906484 1104 62-63 0.4810408800858852 1104 64-65 0.5113758988800186 1104 66-67 0.49689112452671225 1104 68-69 0.47204867739303324 1104 70-71 0.40284665070750236 1104 72-73 0.5113058367884662 1104 74-75 0.560291652942432 1104 76-77 0.5184547742411283 1104 78-79 0.5907541521811623 1104 80-81 0.6040667784874998 1104 82-83 0.4808826527031407 1104 84-85 0.4985867314343899 1104 86-87 0.607101691401617 1104 88-89 0.6958741968567743 1104 90-91 0.5233875742775602 1104 92-93 0.565961196757911 1104 94-95 0.5934458165482503 1104 96-97 0.5754811998826455 1104 98-99 0.6162970832905508 1104 100 0.7958295787099949 1105 1 0.22334134300285768 1105 2 0.1467812703183924 1105 3 0.22332618307468977 1105 4 0.10788383189198214 1105 5 0.09126833761774833 1105 6 0.11401278573193707 1105 7 0.15738191856786443 1105 8 0.15053998133782898 1105 9 0.15344781877439573 1105 10-11 0.19184040438445749 1105 12-13 0.19958956959194296 1105 14-15 0.22438578339114912 1105 16-17 0.262253756303636 1105 18-19 0.2691571973798048 1105 20-21 0.24275291194823012 1105 22-23 0.31545706624951464 1105 24-25 0.33529260012286244 1105 26-27 0.3512749006519087 1105 28-29 0.40495234293833704 1105 30-31 0.37457959085219983 1105 32-33 0.4000096775433235 1105 34-35 0.41894324319881804 1105 36-37 0.4108279665970045 1105 38-39 0.4581042284447321 1105 40-41 0.46674635085283 1105 42-43 0.414088734804551 1105 44-45 0.4684168764748051 1105 46-47 0.4728895006125313 1105 48-49 0.48727073481558847 1105 50-51 0.5445780663274107 1105 52-53 0.47077072356052696 1105 54-55 0.4934053295818259 1105 56-57 0.4533751897773044 1105 58-59 0.4729360634858111 1105 60-61 0.539570301505897 1105 62-63 0.6187306752836292 1105 64-65 0.6380411700425626 1105 66-67 0.6200579282547807 1105 68-69 0.6338329463944561 1105 70-71 0.6331997862608958 1105 72-73 0.6053085126385795 1105 74-75 0.5984328049485015 1105 76-77 0.6216023163617876 1105 78-79 0.6813748047129167 1105 80-81 0.6260290431268487 1105 82-83 0.5811205853260013 1105 84-85 0.6815958133287445 1105 86-87 0.6266342688728379 1105 88-89 0.6219191158279322 1105 90-91 0.7498186131432618 1105 92-93 0.7004132896490347 1105 94-95 0.7535001831320827 1105 96-97 0.7009750191766422 1105 98-99 0.6571139194752931 1105 100 0.8222155582244604 1106 1 0.26990676694175875 1106 2 0.1884223687826747 1106 3 0.2153644315215928 1106 4 0.16571702686287892 1106 5 0.12451528776111331 1106 6 0.10779216088127441 1106 7 0.17280735082886878 1106 8 0.16736752121065024 1106 9 0.14890786483788787 1106 10-11 0.15450840359569185 1106 12-13 0.16108845992064857 1106 14-15 0.2052939090903081 1106 16-17 0.23582640999116222 1106 18-19 0.22982803432210375 1106 20-21 0.2648167503757435 1106 22-23 0.2228025039205761 1106 24-25 0.27405118357204117 1106 26-27 0.2956464540355981 1106 28-29 0.30638461056410193 1106 30-31 0.3475532231343905 1106 32-33 0.3784835138054632 1106 34-35 0.38443872780901955 1106 36-37 0.3588101517822935 1106 38-39 0.356808794541017 1106 40-41 0.3810929506326346 1106 42-43 0.34397412675151884 1106 44-45 0.40632188783116874 1106 46-47 0.4637942984857162 1106 48-49 0.5216648388446856 1106 50-51 0.48919237621672806 1106 52-53 0.5560240125251141 1106 54-55 0.46192252176556536 1106 56-57 0.48122191072322806 1106 58-59 0.5098098518245422 1106 60-61 0.5950861730806665 1106 62-63 0.5412037845489976 1106 64-65 0.6348416588138193 1106 66-67 0.574807619230242 1106 68-69 0.6121037996958734 1106 70-71 0.6360312171955229 1106 72-73 0.5613531453864269 1106 74-75 0.5754117922983539 1106 76-77 0.5600232595365711 1106 78-79 0.6219378131354887 1106 80-81 0.6364605727253192 1106 82-83 0.587274305770972 1106 84-85 0.5809858844264504 1106 86-87 0.6122924588884793 1106 88-89 0.6584028476631971 1106 90-91 0.697694629479308 1106 92-93 0.6903830592093883 1106 94-95 0.7619072286878286 1106 96-97 0.7780882650441008 1106 98-99 0.8020355229545579 1106 100 0.8159998546870888 1107 1 0.18367165872170688 1107 2 0.1656230822180902 1107 3 0.2216901045293369 1107 4 0.14998853480715013 1107 5 0.13753742661553048 1107 6 0.11575717787196993 1107 7 0.12058992956355752 1107 8 0.12185529884945367 1107 9 0.17085753141401483 1107 10-11 0.17020175042024022 1107 12-13 0.20647635336406012 1107 14-15 0.2076023117740604 1107 16-17 0.1880572886849876 1107 18-19 0.16925373738860827 1107 20-21 0.19298260297946257 1107 22-23 0.24741664762256477 1107 24-25 0.2874439661644175 1107 26-27 0.27377915510125916 1107 28-29 0.2832475463777513 1107 30-31 0.1569289398841036 1107 32-33 0.18780195447844505 1107 34-35 0.23897630091536115 1107 36-37 0.30440688883938805 1107 38-39 0.2901352237889441 1107 40-41 0.2656954797942248 1107 42-43 0.2592120510204836 1107 44-45 0.3334819478191733 1107 46-47 0.30071192709510086 1107 48-49 0.3590362243387162 1107 50-51 0.24972325068461743 1107 52-53 0.23732899457676382 1107 54-55 0.3071289081950894 1107 56-57 0.37615943826430964 1107 58-59 0.4891394716630444 1107 60-61 0.5274659602019582 1107 62-63 0.45392305245125897 1107 64-65 0.5193422455048804 1107 66-67 0.5247434504281614 1107 68-69 0.3885296055142575 1107 70-71 0.4980316777897542 1107 72-73 0.4341499866949263 1107 74-75 0.6093776560122208 1107 76-77 0.4287550117065422 1107 78-79 0.4924798003124593 1107 80-81 0.48325245638361736 1107 82-83 0.4825440941975323 1107 84-85 0.4046998330873173 1107 86-87 0.37649081277956853 1107 88-89 0.39034974351709906 1107 90-91 0.25210257845303374 1107 92-93 0.33475898725649245 1107 94-95 0.5232704700306847 1107 96-97 0.5995473169731582 1107 98-99 0.6432571643740275 1107 100 0.8595306102149856 1108 1 0.21288416164135526 1108 2 0.16603117259109723 1108 3 0.2240149823726867 1108 4 0.1516556296602687 1108 5 0.10697049297971972 1108 6 0.10613314063133572 1108 7 0.13762488627441627 1108 8 0.1481763932054534 1108 9 0.13773351112479304 1108 10-11 0.15241756678754115 1108 12-13 0.183926405470487 1108 14-15 0.21265084094505937 1108 16-17 0.2582004169652734 1108 18-19 0.26081853301674585 1108 20-21 0.24253127585414092 1108 22-23 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-0.4129546215250599 2316 72-73 -0.47012904061271144 2316 74-75 -0.5534422327748203 2316 76-77 -0.536870212374609 2316 78-79 -0.39044149604337264 2316 80-81 -0.3282586905472016 2316 82-83 -0.2106017378621523 2316 84-85 -0.2416417662235979 2316 86-87 -0.24446395528861942 2316 88-89 -0.2190164611599279 2316 90-91 -0.16017984590289558 2316 92-93 -0.01155717141067214 2316 94-95 -0.10280655098973313 2316 96-97 -0.04352193769173951 2316 98-99 -0.180651341275329 2316 100 -0.3698638337527882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 15.0 11 34.0 12 92.0 13 245.0 14 788.0 15 2028.0 16 4716.0 17 9628.0 18 17398.0 19 27257.0 20 39596.0 21 54505.0 22 71076.0 23 81453.0 24 86471.0 25 95269.0 26 110985.0 27 133193.0 28 160768.0 29 194059.0 30 234211.0 31 284911.0 32 347180.0 33 436848.0 34 571971.0 35 784527.0 36 1151420.0 37 1807208.0 38 1857022.0 39 298041.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78704982266074 20.369698806477594 13.917594170788636 23.925657200073026 2 14.918702528301239 21.377292348436058 40.069071914458135 23.63493320880457 3 17.252886814920927 26.58073299407574 31.021399134051432 25.144981056951902 4 11.771713947289024 17.719621730950756 38.70561299475634 31.803051327003878 5 12.286958327033608 38.481465267262564 35.96865331801842 13.26292308768541 6 30.56312267993546 38.52653194778233 17.847589389477484 13.062755982804724 7 26.581866439924955 33.02495964096822 22.874036945720544 17.51913697338628 8 25.326544863396972 36.63895141878969 20.431675018174133 17.602828699639204 9 26.032125890434838 15.244594206553238 20.63392021500754 38.08935968800438 10-11 24.167167628649068 26.732854576152317 29.313333441649437 19.78664435354918 12-13 24.785991072045103 24.510461981724365 29.190174646178672 21.513372300051856 14-15 22.059556255999695 26.034650096107974 26.68278094376724 25.223012704125082 16-17 21.62105594640841 28.899296713084404 27.494618427512897 21.985028912994288 18-19 21.406799598526955 27.992703033890354 29.4676612062594 21.132836161323294 20-21 22.22845750897179 27.211819410725347 29.49370117250032 21.066021907802543 22-23 21.944409577062256 27.209700835980357 29.195343874032876 21.65054571292451 24-25 21.72331495814457 27.317170726781352 29.395079909928384 21.56443440514569 26-27 21.743699292322304 27.43314205353916 29.317478762391637 21.505679891746894 28-29 21.686178893803092 27.398888045356458 29.445862377517688 21.469070683322762 30-31 21.975506277557947 27.33976384329492 29.391490941098024 21.293238938049104 32-33 21.721959776896245 27.457017153647158 29.294585138771318 21.526437930685287 34-35 21.934460357176103 27.494543064515675 29.3109677492151 21.260028829093123 36-37 21.985773391317828 27.3940013961682 29.192264682078374 21.4279605304356 38-39 21.823300746855335 27.5920410275389 29.221560500867245 21.363097724738523 40-41 21.981210320394258 27.419241912371735 29.25112368485473 21.348424082379278 42-43 21.790525473372305 27.452967429322438 29.161285517909064 21.595221579396192 44-45 21.856730000267984 27.660686857631273 28.969177584672433 21.51340555742831 46-47 21.851023501284764 27.587385656403445 28.776746370365153 21.784844471946634 48-49 21.80969731368647 27.682536225710592 28.791889548185008 21.71587691241793 50-51 21.94033064984367 27.82897770513931 28.54845850408047 21.682233140936553 52-53 21.92587082118159 27.877939196733053 28.45825534158608 21.737934640499276 54-55 21.84203101672864 27.880376861156886 28.51314439275422 21.76444772936025 56-57 21.923425032810258 27.904000592144573 28.348712675103936 21.82386169994123 58-59 21.885329157608997 27.82283952414254 28.52406852394328 21.76776279430518 60-61 22.01298348496605 28.00565454054404 28.41582650743036 21.565535467059554 62-63 21.79624401977587 27.963181589824444 28.55392549607818 21.6866488943215 64-65 21.77560911211928 27.78681858609656 28.51290973831867 21.92466256346549 66-67 21.780887249660108 27.862301021103917 28.35169247124233 22.005119257993645 68-69 21.733813053553714 27.866952341231478 28.401627338455526 21.99760726675929 70-71 21.74020531824373 27.707931802505335 28.417584237834358 22.13427864141658 72-73 21.731643608803584 27.90505914789991 28.22602789691786 22.137269346378652 74-75 21.677357944711055 27.94420669332829 28.184946623344914 22.19348873861574 76-77 21.62513437664048 27.834236417698595 28.307603256688928 22.233025948971996 78-79 21.569995119735733 27.78360964656167 28.269909644587 22.3764855891156 80-81 21.564345206766404 28.03172660421898 28.045142827773205 22.35878536124141 82-83 21.690611646477667 27.936558969177195 28.117427149986923 22.25540223435822 84-85 21.616942925139767 27.892380001324856 28.073825737386738 22.41685133614864 86-87 21.430580658842285 27.86693077101779 28.24392444033425 22.458564129805676 88-89 21.496244593387594 27.78458066558782 28.19194326795959 22.527231473064994 90-91 21.616143456359886 27.832516498782635 28.076632967974025 22.474707076883455 92-93 21.50864303222983 27.77203873753405 28.087776559113507 22.631541671122612 94-95 21.329812465238813 27.68183964041093 28.141992418921603 22.846355475428652 96-97 21.32580735627955 27.61249042841754 28.197975028397344 22.863727186905567 98-99 21.246513350579107 27.54794132374016 28.11495174354195 23.090593582138787 100 20.475184381768948 27.915863637051004 28.324384617311765 23.284567363868284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2808.0 1 2013.5 2 1868.0 3 2619.5 4 2180.0 5 1931.5 6 3073.0 7 6077.0 8 9612.0 9 11421.0 10 10049.5 11 8008.0 12 8042.5 13 8629.5 14 9310.0 15 9975.0 16 10644.5 17 11570.5 18 12592.5 19 13886.5 20 15782.0 21 18548.5 22 22143.0 23 27139.5 24 33872.0 25 42511.5 26 53734.5 27 66867.5 28 80627.5 29 96768.0 30 116313.0 31 135083.0 32 155201.5 33 178897.5 34 202640.5 35 224086.0 36 248202.0 37 271256.0 38 287429.5 39 299605.0 40 304336.0 41 307106.5 42 311033.0 43 315385.0 44 322099.5 45 330650.5 46 334443.0 47 332581.0 48 332552.5 49 331346.0 50 326050.5 51 311415.0 52 292921.0 53 278432.5 54 261697.0 55 239615.0 56 213838.5 57 188310.5 58 165011.5 59 140468.5 60 115543.0 61 93677.5 62 74253.5 63 57047.5 64 42243.0 65 31016.5 66 22768.0 67 16785.0 68 12544.0 69 9435.0 70 7168.0 71 5320.5 72 3947.0 73 2979.5 74 2257.5 75 1643.5 76 1122.5 77 724.5 78 466.5 79 310.5 80 213.5 81 145.5 82 112.5 83 78.0 84 50.0 85 41.0 86 29.0 87 25.5 88 21.5 89 13.5 90 7.5 91 8.0 92 8.5 93 4.5 94 3.0 95 4.5 96 6.0 97 3.0 98 2.0 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003091522143647915 2 0.06873559452227407 3 7.446732170830744E-4 4 5.641463765780867E-4 5 0.001444214724039902 6 5.415805215149632E-4 7 0.0 8 8.687854199302534E-4 9 3.38487825946852E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.277488257857317E-4 18-19 9.7597323148009E-4 20-21 0.003458217288423671 22-23 5.641463765780867E-6 24-25 5.641463765780867E-6 26-27 1.69243912973426E-4 28-29 9.7597323148009E-4 30-31 0.001652948883373794 32-33 0.002882787984314023 34-35 0.0042085319692725266 36-37 0.0013426683762558463 38-39 0.0013595927675531887 40-41 0.005376314968789166 42-43 0.00683181262036063 44-45 0.005477861316573221 46-47 0.007040546779694521 48-49 0.00906019080784407 50-51 0.008953002996294236 52-53 0.004118268549020033 54-55 0.0024201879555199915 56-57 0.002792524564061529 58-59 0.0033171806942791497 60-61 0.007779578533011815 62-63 0.013990830139136549 64-65 0.004011080737470196 66-67 0.010397217720334136 68-69 0.012591747125222893 70-71 0.005765575968628046 72-73 0.008061651721300859 74-75 0.0030689562885847913 76-77 0.00299561725962964 78-79 0.008157556605319133 80-81 0.006132271113403802 82-83 0.017172615703036957 84-85 0.01828962552866157 86-87 0.023203340468656703 88-89 0.021702711106958993 90-91 0.025031174728769708 92-93 0.034587814348002496 94-95 0.03825476579576006 96-97 0.02350233804824309 98-99 0.018582981644482174 100 0.019338937789096813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8862948.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.68783401811228 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70952063191044 43.39873799013979 2 16.615105698828657 19.834393422901524 3 5.342050717144447 9.565663095637664 4 2.2307617692250177 5.325973528618134 5 1.0364603434150632 3.093203647205697 6 0.5989726883887437 2.145082944355789 7 0.36977820839372766 1.5449882228283824 8 0.23522724319325067 1.12321637186055 9 0.1563053786215688 0.8396576545772154 >10 0.6500289634979908 6.5195522008255296 >50 0.031417549391835733 1.3056823132343591 >100 0.021720248688439938 2.5299143054091857 >500 0.0017850927024353625 0.7205195540276094 >1k 7.843291049952354E-4 0.8096234306803766 >5k 0.0 0.0 >10k+ 8.113749333687854E-5 1.243791317698163 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30473 0.3438246506692807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27934 0.31517729766664543 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18641 0.21032505211584224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.1282927531561734E-5 0.0 4 0.0 0.0 0.0 1.1282927531561734E-5 1.1282927531561734E-5 5 0.0 0.0 0.0 1.1282927531561734E-5 1.1282927531561734E-5 6 0.0 0.0 0.0 1.1282927531561734E-5 1.1282927531561734E-5 7 0.0 0.0 0.0 1.1282927531561734E-5 1.1282927531561734E-5 8 0.0 0.0 0.0 2.2565855063123467E-5 1.1282927531561734E-5 9 0.0 0.0 0.0 2.2565855063123467E-5 3.38487825946852E-5 10-11 0.0 0.0 0.0 3.949024636046607E-5 3.38487825946852E-5 12-13 0.0 0.0 0.0 4.5131710126246935E-5 3.949024636046607E-5 14-15 0.0 0.0 0.0 5.07731738920278E-5 1.4667805791030252E-4 16-17 0.0 0.0 0.0 5.641463765780867E-5 2.030926955681112E-4 18-19 0.0 0.0 0.0 7.333902895515128E-5 2.1437562309967293E-4 20-21 0.0 0.0 0.0 1.0154634778405559E-4 2.1437562309967293E-4 22-23 0.0 0.0 0.0 1.4103659414452165E-4 2.482244056943581E-4 24-25 0.0 0.0 0.0 2.0873415933389208E-4 2.9335611582060505E-4 26-27 0.0 0.0 0.0 3.7797807230731807E-4 2.989975795863859E-4 28-29 0.0 5.641463765780867E-6 0.0 0.0011903488545797628 3.046390433521668E-4 30-31 0.0 2.2565855063123467E-5 0.0 0.00449060515756157 3.1592197088372854E-4 32-33 0.0 2.2565855063123467E-5 0.0 0.012287108081870727 3.4977075347841373E-4 34-35 0.0 2.2565855063123467E-5 0.0 0.02427521858415507 3.4977075347841373E-4 36-37 0.0 3.38487825946852E-5 0.0 0.04513735159001271 3.4977075347841373E-4 38-39 0.0 4.5131710126246935E-5 0.0 0.07677468038851182 3.610536810099755E-4 40-41 0.0 5.641463765780867E-5 0.0 0.11235539235929173 3.610536810099755E-4 42-43 0.0 6.76975651893704E-5 0.0 0.14738888234479092 3.610536810099755E-4 44-45 0.0 6.76975651893704E-5 0.0 0.1908789265151956 3.666951447757563E-4 46-47 0.0 6.76975651893704E-5 0.0 0.24453488839153742 3.7797807230731807E-4 48-49 0.0 6.76975651893704E-5 0.0 0.30550782877209703 4.061853911362224E-4 50-51 0.0 7.333902895515128E-5 0.0 0.37074007429582123 4.174683186677841E-4 52-53 5.641463765780867E-6 7.898049272093214E-5 0.0 0.43447169045784767 4.5131710126246935E-4 54-55 1.1282927531561734E-5 7.898049272093214E-5 0.0 0.5097118927020671 5.020902751544971E-4 56-57 1.1282927531561734E-5 9.026342025249387E-5 0.0 0.6001953300414264 5.190146664518397E-4 58-59 1.1282927531561734E-5 9.026342025249387E-5 0.0 0.703981338940497 5.246561302176206E-4 60-61 1.1282927531561734E-5 9.026342025249387E-5 0.0 0.8163762215461492 5.302975939834014E-4 62-63 1.1282927531561734E-5 9.026342025249387E-5 0.0 0.9319528897157019 5.641463765780867E-4 64-65 1.1282927531561734E-5 9.026342025249387E-5 0.0 1.0505646653912444 5.641463765780867E-4 66-67 1.1282927531561734E-5 9.026342025249387E-5 0.0 1.1900724228552395 5.641463765780867E-4 68-69 1.1282927531561734E-5 9.026342025249387E-5 0.0 1.3533420257007036 5.641463765780867E-4 70-71 1.1282927531561734E-5 9.026342025249387E-5 0.0 1.534963310176253 5.641463765780867E-4 72-73 1.69243912973426E-5 9.026342025249387E-5 0.0 1.7194786655636478 6.995415069568275E-4 74-75 2.2565855063123467E-5 9.026342025249387E-5 0.0 1.9047330527043598 6.995415069568275E-4 76-77 2.2565855063123467E-5 9.026342025249387E-5 0.0 2.111131646039219 7.108244344883892E-4 78-79 2.2565855063123467E-5 9.590488401827474E-5 0.0 2.353031970852136 7.333902895515126E-4 80-81 2.2565855063123467E-5 1.015463477840556E-4 0.0 2.6166575726270764 7.446732170830743E-4 82-83 2.2565855063123467E-5 1.015463477840556E-4 0.0 2.8938565362224846 8.236537098040065E-4 84-85 2.2565855063123467E-5 1.015463477840556E-4 0.0 3.165402753124581 8.292951735697873E-4 86-87 2.2565855063123467E-5 1.015463477840556E-4 0.0 3.454076454019588 8.405781011013491E-4 88 2.2565855063123467E-5 1.015463477840556E-4 0.0 3.6875427905026634 8.4621956486713E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14675 0.0 49.8601 1 GTATCAA 25135 0.0 36.500607 1 TCAACGC 30480 0.0 29.616425 4 ATCAACG 30595 0.0 29.42864 3 CAACGCA 30880 0.0 29.278444 5 AACGCAG 32025 0.0 28.333878 6 TATCAAC 32655 0.0 27.691338 2 GTACATG 28670 0.0 25.619757 1 TACCTGG 6975 0.0 25.348833 2 ACGCAGA 36780 0.0 24.555824 7 GTACCTG 7710 0.0 24.335495 1 TACATGG 29355 0.0 24.28463 2 CGCAGAG 37430 0.0 24.00385 8 ACATGGG 28870 0.0 23.325155 3 GCAGAGT 41800 0.0 21.235556 9 GAGTACT 26000 0.0 20.784746 12-13 CATGGGG 15800 0.0 20.402681 4 TATAACG 825 0.0 19.379354 2 AGAGTAC 38840 0.0 19.32734 10-11 ACCTGGG 9230 0.0 18.939407 3 >>END_MODULE