FastQCFastQC Report
Mon 6 Jun 2016
ERR1211491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875965
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169190.43651064960596914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143270.36963698072609014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95680.2468546542602939No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86200.22239622906811596No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50680.13075453467717071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG61100.058.560262
GTACCTG66350.055.0749171
ACCTGGG68100.051.6856543
GGTATCA81900.043.410631
TATAACG5200.038.879862
CCTGGGG62000.037.2132034
TAACGCA5450.037.0748374
GTATCAA133750.035.7359471
CTGGGGG42400.030.1438795
ATAACGC7000.029.53863
TCAACGC163350.028.6802734
ATCAACG164400.028.5561073
CAACGCA164700.028.5300465
GTACATG142200.028.3138921
TATCAAC167500.028.0140112
AACGCAG171350.027.8280496
CATGGGG91300.027.277994
TACATGG146800.026.9357762
ATGGGGG62100.026.5589925
TGGGGGG73850.026.5268926
Produced by FastQC (version 0.11.5)