##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3875965 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37967473906498 31.0 31.0 34.0 28.0 34.0 2 31.402624894703642 31.0 31.0 34.0 28.0 34.0 3 31.493649452458936 33.0 31.0 34.0 28.0 34.0 4 35.04038555559712 37.0 35.0 37.0 32.0 37.0 5 35.13372205373371 37.0 35.0 37.0 32.0 37.0 6 35.24942665890946 37.0 35.0 37.0 32.0 37.0 7 35.1881270857709 37.0 35.0 37.0 32.0 37.0 8 35.157763550496455 37.0 35.0 37.0 32.0 37.0 9 36.7317197136713 39.0 37.0 39.0 32.0 39.0 10-11 36.71013966844386 39.0 37.0 39.0 32.0 39.0 12-13 36.64478523412879 39.0 37.0 39.0 32.0 39.0 14-15 37.83725549637316 40.0 38.0 41.0 32.0 41.0 16-17 37.815761881234735 40.0 38.0 41.0 32.0 41.0 18-19 37.82522584698262 40.0 38.0 41.0 32.0 41.0 20-21 37.775031121282055 40.0 38.0 41.0 32.0 41.0 22-23 37.625422185184846 40.0 37.0 41.0 32.0 41.0 24-25 37.5385091196644 40.0 37.0 41.0 32.0 41.0 26-27 37.39243491620796 40.0 37.0 41.0 31.0 41.0 28-29 37.21675750426023 40.0 36.5 41.0 31.0 41.0 30-31 37.02121020184651 39.5 36.0 41.0 30.0 41.0 32-33 36.82343571213879 39.0 36.0 41.0 30.0 41.0 34-35 36.63989264608942 39.0 35.0 41.0 30.0 41.0 36-37 36.45699354870335 39.0 35.0 41.0 29.5 41.0 38-39 36.32528389704241 39.0 35.0 40.0 29.0 41.0 40-41 36.2804683478824 39.0 35.0 40.0 29.0 41.0 42-43 36.216682555183034 39.0 35.0 40.0 28.5 41.0 44-45 36.036098752181715 39.0 35.0 40.0 27.5 41.0 46-47 35.96680568580986 39.0 35.0 40.0 27.0 41.0 48-49 35.92070594032712 39.0 35.0 41.0 27.0 41.0 50-51 34.926478180272525 38.0 34.0 40.0 25.5 40.5 52-53 35.12899226386203 38.0 34.0 39.5 26.0 40.5 54-55 35.70421882034539 39.0 35.0 40.5 27.0 41.0 56-57 35.65907057984269 39.0 35.0 41.0 26.0 41.0 58-59 35.489887034583646 38.5 34.5 40.5 26.0 41.0 60-61 35.27490973215703 38.0 34.0 40.0 26.0 41.0 62-63 34.993157961952704 37.5 34.0 40.0 26.0 41.0 64-65 34.706356739547445 37.0 34.0 40.0 25.5 41.0 66-67 34.39485637770207 36.5 33.5 39.5 25.0 41.0 68-69 34.09790787584511 36.0 33.0 39.0 25.0 41.0 70-71 33.74537012073122 35.5 33.0 39.0 24.5 41.0 72-73 33.39885370481932 35.0 33.0 38.5 24.0 40.0 74-75 33.03314245613673 35.0 32.0 37.0 23.5 39.5 76-77 32.69835615130684 35.0 32.0 37.0 23.0 39.0 78-79 32.38630044388946 35.0 32.0 36.0 23.0 39.0 80-81 32.068305828355 35.0 31.5 36.0 22.0 38.0 82-83 31.774915279162737 35.0 31.0 35.5 21.0 37.0 84-85 31.52378078749421 35.0 31.0 35.0 20.0 37.0 86-87 31.241379888621285 34.5 31.0 35.0 20.0 36.0 88-89 31.02956425045118 34.0 31.0 35.0 20.0 36.0 90-91 30.806228384415235 34.0 30.0 35.0 19.0 36.0 92-93 30.649861518357362 34.0 30.0 35.0 18.5 35.5 94-95 30.491320226059834 34.0 30.0 35.0 18.0 35.0 96-97 30.383378074879417 34.0 30.0 35.0 18.0 35.0 98-99 30.19922651520331 34.0 29.0 35.0 17.5 35.0 100 28.830441451354694 33.0 27.0 35.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3757276382056176 1101 2 0.34457460918461535 1101 3 0.34833630610760835 1101 4 0.2607255161797468 1101 5 0.267856150771145 1101 6 0.2375733065616572 1101 7 0.25627942579949803 1101 8 0.29042666947202633 1101 9 0.20880643845094227 1101 10-11 0.3240762535579975 1101 12-13 0.29130287341892114 1101 14-15 0.34735074495470286 1101 16-17 0.3309246139414057 1101 18-19 0.25762762388161065 1101 20-21 0.2297058547112556 1101 22-23 0.2953072442321911 1101 24-25 0.31757603440680526 1101 26-27 0.3301348644325728 1101 28-29 0.23374525239281496 1101 30-31 0.33813570984493424 1101 32-33 0.30128847275063464 1101 34-35 0.28138254841626065 1101 36-37 0.26269045273926395 1101 38-39 0.26145396292282186 1101 40-41 0.09268947337277211 1101 42-43 0.03238107203586793 1101 44-45 0.06823302771313422 1101 46-47 0.0857483469926521 1101 48-49 0.22016135097769762 1101 50-51 0.21274254238003465 1101 52-53 0.2782461500169262 1101 54-55 0.2535611436903338 1101 56-57 0.24565836619645864 1101 58-59 0.2694015658209423 1101 60-61 0.2302323096399661 1101 62-63 0.2324244488913223 1101 64-65 0.17807375184194285 1101 66-67 0.23810903793275884 1101 68-69 0.15022468237126674 1101 70-71 0.06001928762164255 1101 72-73 0.18032269774441545 1101 74-75 0.1233196654896247 1101 76-77 0.07386193469579894 1101 78-79 -0.04662998015304254 1101 80-81 0.07639472583178986 1101 82-83 -0.0625817743616679 1101 84-85 -0.216452371235345 1101 86-87 -0.25423517766382986 1101 88-89 -0.12724076867239376 1101 90-91 -0.29067751881645876 1101 92-93 -0.3207306692095706 1101 94-95 -0.3802852046865084 1101 96-97 -0.516675719674037 1101 98-99 -0.500797976936834 1101 100 -0.43212099941817783 1102 1 0.39106004824682117 1102 2 0.38282993096591156 1102 3 0.40533236369490666 1102 4 0.3331333100746363 1102 5 0.3998891786163412 1102 6 0.32906520490031 1102 7 0.2643914693111711 1102 8 0.30376013330347007 1102 9 0.3342644165103863 1102 10-11 0.34471065388279243 1102 12-13 0.39021621171694676 1102 14-15 0.4548983985735049 1102 16-17 0.40711678985169897 1102 18-19 0.4646251360541598 1102 20-21 0.4290269689516748 1102 22-23 0.4022623841741151 1102 24-25 0.41644827661590256 1102 26-27 0.40283544092417856 1102 28-29 0.29673311974588756 1102 30-31 0.30912838695446965 1102 32-33 0.28815716382506196 1102 34-35 0.38910269746095594 1102 36-37 0.3766000242562981 1102 38-39 0.4072776215518914 1102 40-41 0.37774438315495473 1102 42-43 0.46048819591310775 1102 44-45 0.36700892122322415 1102 46-47 0.2937826161560295 1102 48-49 0.454028675715314 1102 50-51 0.3623203828065229 1102 52-53 0.45981059052287776 1102 54-55 0.4831077013413321 1102 56-57 0.4580715588196611 1102 58-59 0.5259974610570666 1102 60-61 0.4894087418548949 1102 62-63 0.488491934941905 1102 64-65 0.39535804766370575 1102 66-67 0.2535315078899032 1102 68-69 0.39796804746178793 1102 70-71 0.3488012611899336 1102 72-73 0.40357255050145824 1102 74-75 0.30509174939226824 1102 76-77 0.289922459285215 1102 78-79 0.2062892210428089 1102 80-81 0.2240969623890976 1102 82-83 0.2498833074811948 1102 84-85 0.19227102526556195 1102 86-87 0.0554379386374535 1102 88-89 0.08451577874567917 1102 90-91 0.10352176282390957 1102 92-93 0.09758917775724285 1102 94-95 0.031181058344465384 1102 96-97 0.002029597792169824 1102 98-99 -0.061018590475487855 1102 100 -0.075071581803833 1103 1 0.43312695586464045 1103 2 0.43130443161393117 1103 3 0.39702379828280954 1103 4 0.39400750949437224 1103 5 0.30555998992609545 1103 6 0.294026170341688 1103 7 0.37604777633739417 1103 8 0.34627262743116916 1103 9 0.380864502912857 1103 10-11 0.43849805659148444 1103 12-13 0.34672549290033317 1103 14-15 0.4794729372291755 1103 16-17 0.49515494622127676 1103 18-19 0.510753963081612 1103 20-21 0.4719531798256398 1103 22-23 0.5437951191427928 1103 24-25 0.5449031950647267 1103 26-27 0.5768233988749785 1103 28-29 0.5685102013726109 1103 30-31 0.5884512252992238 1103 32-33 0.5217144314295652 1103 34-35 0.5721419633704627 1103 36-37 0.5662974149489131 1103 38-39 0.4796700847069033 1103 40-41 0.3974493399655259 1103 42-43 0.3573886253286602 1103 44-45 0.4757228818321977 1103 46-47 0.5335904886958573 1103 48-49 0.5099379790894929 1103 50-51 0.38519136456049097 1103 52-53 0.3909509496833081 1103 54-55 0.47441876439860664 1103 56-57 0.47461609740450683 1103 58-59 0.3967865209934658 1103 60-61 0.4277165518355588 1103 62-63 0.4688551440146398 1103 64-65 0.3352189184400771 1103 66-67 0.25125488542384034 1103 68-69 0.34474288640144124 1103 70-71 0.3598934143819861 1103 72-73 0.35686800209600733 1103 74-75 0.3314424371889828 1103 76-77 0.3503746794273752 1103 78-79 0.315008493279052 1103 80-81 0.29544893677997663 1103 82-83 0.2624488470598969 1103 84-85 0.12579571488947394 1103 86-87 0.15770653464452877 1103 88-89 0.20073683922976926 1103 90-91 0.13587813052071596 1103 92-93 0.14745650511165564 1103 94-95 0.11330943919522696 1103 96-97 0.007893634298394403 1103 98-99 0.08924882044852112 1103 100 0.06295164299131528 1104 1 0.45157406962647073 1104 2 0.4647443999748013 1104 3 0.3776024492648382 1104 4 0.4222949831707794 1104 5 0.34483423385314893 1104 6 0.3213761682766645 1104 7 0.31829857872192235 1104 8 0.3405065731996402 1104 9 0.46877266596855094 1104 10-11 0.46780603926607256 1104 12-13 0.3996079740291947 1104 14-15 0.46271465287789226 1104 16-17 0.49610677439088136 1104 18-19 0.5112654609172367 1104 20-21 0.5295883418583358 1104 22-23 0.47189437329223693 1104 24-25 0.5219096320882173 1104 26-27 0.6279566406554977 1104 28-29 0.5876230948156476 1104 30-31 0.5850362123241979 1104 32-33 0.4754211473712857 1104 34-35 0.5241032240315349 1104 36-37 0.5675521036790698 1104 38-39 0.5335917248818518 1104 40-41 0.4175209269581046 1104 42-43 0.2637010286592485 1104 44-45 0.4206791844996616 1104 46-47 0.41661692873363165 1104 48-49 0.44877193052325737 1104 50-51 0.4929656397331357 1104 52-53 0.5002533124768007 1104 54-55 0.49750265847671216 1104 56-57 0.5371877919727268 1104 58-59 0.4874961915352358 1104 60-61 0.5517021514037808 1104 62-63 0.611199472335052 1104 64-65 0.47289589658308273 1104 66-67 0.35831075534532886 1104 68-69 0.31109219952828226 1104 70-71 0.32427468796078074 1104 72-73 0.40404989336438746 1104 74-75 0.39377043779789034 1104 76-77 0.44073813480446233 1104 78-79 0.3734653115527635 1104 80-81 0.3153052914080945 1104 82-83 0.3563994634629033 1104 84-85 0.1561282609204966 1104 86-87 0.27900166208435806 1104 88-89 0.25630998169040353 1104 90-91 0.22140000643226898 1104 92-93 0.3214253682890629 1104 94-95 0.37016473731164723 1104 96-97 0.2828573180601808 1104 98-99 0.20243042023557578 1104 100 0.22135844670177818 1105 1 0.3646907264664634 1105 2 0.40451830707998226 1105 3 0.3194662614979542 1105 4 0.34674027242238026 1105 5 0.3109333687750322 1105 6 0.2693859525993858 1105 7 0.35996962934218857 1105 8 0.2591549523279184 1105 9 0.3791787160230484 1105 10-11 0.3710872548354942 1105 12-13 0.300559986776733 1105 14-15 0.4681661405435946 1105 16-17 0.4421954771574974 1105 18-19 0.3821247753793884 1105 20-21 0.4482674086618488 1105 22-23 0.5125900355934832 1105 24-25 0.42268918175526693 1105 26-27 0.43420607405017364 1105 28-29 0.3837255780854818 1105 30-31 0.5707116025622412 1105 32-33 0.5352208387409476 1105 34-35 0.512940520128069 1105 36-37 0.5897011607485538 1105 38-39 0.5868219377004635 1105 40-41 0.5277026674770227 1105 42-43 0.5319206571249637 1105 44-45 0.5205433315675663 1105 46-47 0.38093923898784254 1105 48-49 0.3447155657836092 1105 50-51 0.4281171227005629 1105 52-53 0.4765955136384008 1105 54-55 0.4705580524601416 1105 56-57 0.4306300094790245 1105 58-59 0.5376381985455865 1105 60-61 0.5484131108907562 1105 62-63 0.5210758911644149 1105 64-65 0.460249762039048 1105 66-67 0.45867864349726517 1105 68-69 0.5302281735567576 1105 70-71 0.5179069705348809 1105 72-73 0.4613001089225648 1105 74-75 0.5459645155894108 1105 76-77 0.4974118191055368 1105 78-79 0.5090035915739151 1105 80-81 0.4206401162782498 1105 82-83 0.5078645642144259 1105 84-85 0.35798004292319163 1105 86-87 0.4032158590797117 1105 88-89 0.4140396694271793 1105 90-91 0.3086810486287348 1105 92-93 0.379155171828387 1105 94-95 0.402548340861955 1105 96-97 0.3680865872534582 1105 98-99 0.42634118146859024 1105 100 0.5117962001852838 1106 1 0.40309010404028456 1106 2 0.36095569321035725 1106 3 0.29967346092867686 1106 4 0.3167221164456535 1106 5 0.315895893381807 1106 6 0.3432045423930745 1106 7 0.3622922792192327 1106 8 0.4024360284358295 1106 9 0.4045195395850101 1106 10-11 0.3838556820465229 1106 12-13 0.37744099480396187 1106 14-15 0.5270541831235747 1106 16-17 0.5092199283415795 1106 18-19 0.5383110096592674 1106 20-21 0.46307019180378006 1106 22-23 0.49878957019502224 1106 24-25 0.5820802329803385 1106 26-27 0.5730150692724578 1106 28-29 0.6142914530829913 1106 30-31 0.6184818022847622 1106 32-33 0.6830866176066337 1106 34-35 0.6239880264812996 1106 36-37 0.6333223131776649 1106 38-39 0.683792471374737 1106 40-41 0.6547027772227167 1106 42-43 0.5815167185063146 1106 44-45 0.5478601980830717 1106 46-47 0.6265407542680421 1106 48-49 0.7484915397315817 1106 50-51 0.6888691277590198 1106 52-53 0.7524435021959732 1106 54-55 0.6371554654167468 1106 56-57 0.7082690918515766 1106 58-59 0.6365983664959884 1106 60-61 0.628828370347982 1106 62-63 0.7002814552375725 1106 64-65 0.6995871438946466 1106 66-67 0.644069415451682 1106 68-69 0.5778346006619799 1106 70-71 0.6161259602405522 1106 72-73 0.4979696946218226 1106 74-75 0.6290672476031531 1106 76-77 0.6423997905030774 1106 78-79 0.4764402489612678 1106 80-81 0.47032590294682564 1106 82-83 0.5622437842213976 1106 84-85 0.588745445303605 1106 86-87 0.6384896610666608 1106 88-89 0.6048392238122666 1106 90-91 0.7034799822118316 1106 92-93 0.6015005806852614 1106 94-95 0.6424104831183257 1106 96-97 0.5765321335913534 1106 98-99 0.5591243143778506 1106 100 0.6999720089620283 1107 1 0.2580699122961363 1107 2 0.30760280850343946 1107 3 0.1945774110784093 1107 4 0.20206110950786638 1107 5 0.20307846551525444 1107 6 0.21063361322876517 1107 7 0.23660294419914152 1107 8 0.32931060335402407 1107 9 0.2944118541059737 1107 10-11 0.36884262422163516 1107 12-13 0.32214497729718516 1107 14-15 0.378891568760082 1107 16-17 0.4079761375466333 1107 18-19 0.32399247672338305 1107 20-21 0.4590130332828224 1107 22-23 0.4453350099346167 1107 24-25 0.4805337093896611 1107 26-27 0.4520083723344186 1107 28-29 0.5025242138294601 1107 30-31 0.4523754311902053 1107 32-33 0.42827380270201587 1107 34-35 0.46655229196861114 1107 36-37 0.48355990021548934 1107 38-39 0.5813413048871823 1107 40-41 0.5079226751228063 1107 42-43 0.48526368917122653 1107 44-45 0.501518568223382 1107 46-47 0.39902991862459913 1107 48-49 0.4914802848843536 1107 50-51 0.35829701496052024 1107 52-53 0.430856400138488 1107 54-55 0.5030428871242734 1107 56-57 0.5255515847319927 1107 58-59 0.5302113869308798 1107 60-61 0.5221117050767461 1107 62-63 0.5388019813125027 1107 64-65 0.5841579216638877 1107 66-67 0.6016016069756205 1107 68-69 0.5827857929555122 1107 70-71 0.5578802047504752 1107 72-73 0.4899760348638935 1107 74-75 0.46454312704221223 1107 76-77 0.5304999658690406 1107 78-79 0.5042268644900361 1107 80-81 0.5369368669319954 1107 82-83 0.5943885133217073 1107 84-85 0.46426595188068376 1107 86-87 0.5331912350019365 1107 88-89 0.5329330451729639 1107 90-91 0.48909383761348124 1107 92-93 0.5884210214284273 1107 94-95 0.5536960681283745 1107 96-97 0.5248101866966159 1107 98-99 0.502448307858689 1107 100 0.6037682403716893 1108 1 0.2759960459035895 1108 2 0.28432522579045383 1108 3 0.28664082354833553 1108 4 0.2214135113483664 1108 5 0.2954343479996453 1108 6 0.29674940868071076 1108 7 0.2443453874520003 1108 8 0.28649868348404794 1108 9 0.29410891926803373 1108 10-11 0.2658837498878128 1108 12-13 0.2714223594343892 1108 14-15 0.3243061774356306 1108 16-17 0.3094620334717888 1108 18-19 0.2842660446504439 1108 20-21 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86-87 0.3347457515617016 2316 88-89 0.14321179444133492 2316 90-91 0.19801414354201796 2316 92-93 0.25330103455582886 2316 94-95 0.27263898877866666 2316 96-97 0.20217506893423476 2316 98-99 0.33814897422452006 2316 100 -0.028813194871567305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 6.0 1 22.0 2 50.0 3 71.0 4 75.0 5 68.0 6 168.0 7 1060.0 8 2351.0 9 2836.0 10 4079.0 11 6048.0 12 8286.0 13 9627.0 14 10736.0 15 11640.0 16 13912.0 17 16448.0 18 20316.0 19 24454.0 20 27758.0 21 32752.0 22 37547.0 23 39940.0 24 40684.0 25 40682.0 26 43433.0 27 48968.0 28 56015.0 29 66414.0 30 80171.0 31 99791.0 32 124797.0 33 163886.0 34 230899.0 35 328146.0 36 494972.0 37 796741.0 38 799179.0 39 190733.0 40 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.508216098933275 17.54246823571854 12.07688757333449 23.872428092013692 2 16.392802050181725 20.08333060006761 40.12358321063768 23.40028413911298 3 18.350316983693375 26.94491722809277 30.20632005997721 24.498445728236646 4 12.290037091597561 16.134690089533933 37.60332327500328 33.97194954386523 5 12.489319131574105 36.99145711174866 34.42528734149023 16.093936415187002 6 29.851538384533693 35.96934273574751 19.72476472464088 14.454354155077912 7 26.975966916970428 32.59781661912409 22.889248841020645 17.536967622884838 8 26.731898975924643 34.342788010389626 20.95413346804999 17.971179545635742 9 27.765629688571153 15.322640778723049 20.43358486973335 36.47814466297245 10-11 25.046338939932244 25.37571695060878 29.304670789697067 20.273273319761905 12-13 25.323708337069245 23.686869827107422 28.965747234834726 22.023674600988603 14-15 22.882104814156747 24.92051841053078 26.888794931241538 25.308581844070932 16-17 22.26228870755977 27.24948889106487 27.592425666821867 22.895796734553493 18-19 22.01785159726006 26.580909878992536 29.37147829241799 22.02976023132941 20-21 22.801128601649758 26.102100051661342 29.22265677617736 21.87411457051154 22-23 22.486707446114483 25.83541534625169 29.274308315494423 22.403568892139404 24-25 22.03003535666051 26.01527184787192 29.25884935771228 22.695843437755297 26-27 21.958899519932334 26.01207890546998 29.176815096606347 22.85220647799134 28-29 22.12858302512271 26.476594810138685 29.13271381954804 22.262108345190565 30-31 22.93636063959973 26.14315840266502 29.22027897819382 21.70020197954143 32-33 22.64353538688664 26.21684819991198 29.0846334968997 22.054982916301686 34-35 22.70893274337128 26.31601832644782 28.997439979644735 21.977608950536165 36-37 22.527236589956743 26.42150509997373 29.036470631345935 22.01478767872359 38-39 22.61857283587116 26.71598451951866 28.571268211145096 22.09417443346509 40-41 22.61219840380535 26.312566355420923 28.891582532595123 22.1836527081786 42-43 22.15574510917256 26.54979868889692 28.697684922314558 22.59677127961596 44-45 21.997241732456306 26.787753932183062 28.6948738473935 22.520130487967133 46-47 22.088451505136685 26.315925291400642 28.54331546827837 23.052307735184304 48-49 22.084314454310093 26.506735400074806 28.6422257610168 22.766724384598305 50-51 22.136660425315828 26.915144936913244 28.490310854364083 22.45788378340685 52-53 22.358322620652654 26.8589646073159 28.08197145839938 22.700741313632065 54-55 22.042343245114584 26.831152187472824 28.553857842331425 22.57264672508117 56-57 22.300166908203582 26.84749937035248 28.046823066982107 22.805510654461827 58-59 22.336346830305533 27.035639506053684 28.224478964984247 22.40353469865654 60-61 22.7140900606283 27.08606080688571 28.182895505278427 22.01695362720756 62-63 22.66499756320187 27.01109414301114 28.20001414581965 22.123894147967338 64-65 22.41006180946004 26.582455218498424 28.176695127570806 22.83078784447073 66-67 22.222596398003294 26.824558524188785 28.135580142775158 22.817264935032764 68-69 21.968990301517373 26.8228769141377 28.33170898384197 22.876423800502955 70-71 22.012198356147554 26.690501911986054 28.409527783129896 22.8877719487365 72-73 21.85233134878386 27.264739002965854 27.85836165362088 23.024567994629404 74-75 22.157835067045994 27.1374931682838 27.793221776059085 22.911449988611118 76-77 21.969916907243153 26.81579441078051 28.114107446400283 23.100181235576056 78-79 21.900574107650765 26.96920266037488 28.11696638611768 23.01325684585667 80-81 22.223231522725648 27.07589099657787 27.686073364849317 23.014804115847166 82-83 22.40179405824651 27.180095976077844 27.82285674693661 22.595253218739035 84-85 22.190129557254117 27.500373203029028 27.468710619338978 22.84078662037788 86-87 22.09547926463021 27.409174213440146 27.629616382885274 22.865730139044366 88-89 21.846990312306666 27.167693152296835 27.91771686027646 23.06759967512004 90-91 22.52837777016174 27.05467856420251 27.77520707823552 22.64173658740023 92-93 22.29488410107022 27.184573631217646 27.69752225158031 22.823020016131824 94-95 22.24278305675986 26.952372522754185 27.6304112720281 23.174433148457858 96-97 22.081534399671366 27.09323112396928 27.612913513909632 23.212320962449724 98-99 22.09213523593131 27.006930560850943 27.526087178107606 23.37484702511014 100 22.114395359389704 27.045534207568917 27.659397086145226 23.18067334689615 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2836.0 1 2049.0 2 3816.5 3 5641.5 4 3454.0 5 2196.5 6 2912.0 7 5105.0 8 8013.0 9 9071.0 10 7298.5 11 5272.5 12 5042.5 13 5284.0 14 5512.0 15 5844.5 16 6073.0 17 6411.0 18 6809.0 19 7143.5 20 7734.0 21 8516.5 22 9613.0 23 11493.5 24 13829.5 25 16567.5 26 20332.0 27 24691.0 28 29240.5 29 34633.0 30 40880.5 31 47177.5 32 54054.5 33 61687.5 34 69336.5 35 76039.5 36 84290.5 37 92776.5 38 99397.5 39 105449.0 40 108856.0 41 111774.5 42 115430.0 43 119161.5 44 123637.5 45 129350.0 46 134420.5 47 138987.5 48 145543.0 49 152031.5 50 156726.5 51 155988.0 52 149529.0 53 143127.5 54 137350.0 55 128167.5 56 117820.5 57 109663.5 58 100370.0 59 87497.0 60 69472.0 61 52807.0 62 41812.5 63 32345.5 64 24277.5 65 18131.5 66 13659.5 67 10414.5 68 7916.5 69 6085.0 70 4775.5 71 3720.5 72 2936.0 73 2279.5 74 1764.0 75 1367.0 76 1035.0 77 742.5 78 542.5 79 456.0 80 363.5 81 276.5 82 244.5 83 201.5 84 160.0 85 134.0 86 111.5 87 104.0 88 77.5 89 46.0 90 39.0 91 31.0 92 23.5 93 15.5 94 10.5 95 12.5 96 10.0 97 5.0 98 5.0 99 5.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16426876919683228 2 0.10108450411703924 3 0.02992803082587175 4 0.03181143276577575 5 0.02698682779643263 6 0.002089802152496217 7 0.0036378037469378594 8 0.004540804677028817 9 0.004747204889621036 10-11 0.00451500465045479 12-13 0.007546507772903006 14-15 0.017969718508810066 16-17 0.008346308596697855 18-19 0.01613791662205412 20-21 0.0187566193193179 22-23 0.04410514542829979 24-25 0.015454215917842396 26-27 0.009429909712807005 28-29 0.026948127756571585 30-31 0.01477051521363067 32-33 0.020111120714454336 34-35 0.020652921272508912 36-37 0.021852622508201185 38-39 0.01967252026269587 40-41 0.025774226547453344 42-43 0.03521703627354736 44-45 0.03693273804072018 46-47 0.02069162131236995 48-49 0.01311931351289292 50-51 0.012087312449931824 52-53 0.02282012350472721 54-55 0.026161226946063754 56-57 0.020149820754315376 58-59 0.02604512682648063 60-61 0.02688362769013652 62-63 0.052348253918701536 64-65 0.03185013280563679 66-67 0.020149820754315376 68-69 0.027876928713236573 70-71 0.04103494226599053 72-73 0.039577240764557986 74-75 0.040570541787658045 76-77 0.02316842386347658 78-79 0.016679717180108695 80-81 0.02306522375718047 82-83 0.018795319359178938 84-85 0.018795319359178938 86-87 0.020614221232647868 88-89 0.01773751826964382 90-91 0.028405829258004136 92-93 0.0261999269859248 94-95 0.019195219771076365 96-97 0.01344181384506826 98-99 0.019027519598345186 100 0.04249264376742308 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3875965.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.577878559447065 #Duplication Level Percentage of deduplicated Percentage of total 1 77.21138376701884 49.089359805463864 2 14.731650873539115 18.73214220436084 3 3.993841963941974 7.617599981073791 4 1.5282989615708371 3.886640231251189 5 0.7144769645817975 2.2712464843851943 6 0.448991169128652 1.7127543615075358 7 0.3001854800417309 1.335960919678174 8 0.20459075217611308 1.040595679699108 9 0.14707104018384967 0.8415418259178319 >10 0.6659183639898023 7.277323348686263 >50 0.034763644921431094 1.5147902210556892 >100 0.01718313740092563 2.0185217506676887 >500 0.0010153385772600015 0.4552836001649668 >1k 3.867956478561623E-4 0.5308721737131056 >5k 1.4504836794606085E-4 0.714879170345801 >10k+ 9.669891196404057E-5 0.9604882420289343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16919 0.43651064960596914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14327 0.36963698072609014 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9568 0.2468546542602939 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8620 0.22239622906811596 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5068 0.13075453467717071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5800026574027372E-5 2 0.0 2.5800026574027372E-5 0.0 0.0 2.5800026574027372E-5 3 0.0 2.5800026574027372E-5 0.0 0.0 2.5800026574027372E-5 4 0.0 2.5800026574027372E-5 0.0 0.0 2.5800026574027372E-5 5 0.0 2.5800026574027372E-5 0.0 0.0 2.5800026574027372E-5 6 0.0 2.5800026574027372E-5 0.0 0.0 5.1600053148054744E-5 7 0.0 2.5800026574027372E-5 0.0 0.0 5.1600053148054744E-5 8 0.0 2.5800026574027372E-5 0.0 0.0 5.1600053148054744E-5 9 0.0 2.5800026574027372E-5 0.0 2.5800026574027372E-5 7.740007972208211E-5 10-11 0.0 2.5800026574027372E-5 0.0 2.5800026574027372E-5 7.740007972208211E-5 12-13 0.0 2.5800026574027372E-5 0.0 2.5800026574027372E-5 1.4190014615715054E-4 14-15 0.0 2.5800026574027372E-5 0.0 2.5800026574027372E-5 1.935001993052053E-4 16-17 0.0 2.5800026574027372E-5 0.0 2.5800026574027372E-5 2.0640021259221898E-4 18-19 0.0 2.5800026574027372E-5 0.0 7.740007972208211E-5 2.3220023916624633E-4 20-21 0.0 2.5800026574027372E-5 0.0 9.03000930090958E-5 2.5800026574027374E-4 22-23 0.0 2.5800026574027372E-5 0.0 1.806001860181916E-4 3.0960031888832844E-4 24-25 0.0 2.5800026574027372E-5 0.0 2.9670030560131476E-4 3.3540034546235585E-4 26-27 0.0 2.5800026574027372E-5 0.0 6.192006377766569E-4 3.741003853233969E-4 28-29 0.0 2.5800026574027372E-5 0.0 0.0017544018070338612 3.8700039861041055E-4 30-31 0.0 2.5800026574027372E-5 0.0 0.006050106231609419 3.8700039861041055E-4 32-33 1.2900013287013686E-5 2.5800026574027372E-5 0.0 0.015492915957703437 3.8700039861041055E-4 34-35 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.03027633118462112 4.386004517584653E-4 36-37 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.05303195462291326 4.9020050490652E-4 38-39 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.09188679464339848 4.9020050490652E-4 40-41 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.14217104643617784 5.289005447675611E-4 42-43 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.18926899494706478 5.547005713415885E-4 44-45 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.24444235177562235 5.805005979156159E-4 46-47 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.3113160206555013 5.934006112026295E-4 48-49 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.3857619973348573 6.192006377766569E-4 50-51 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.46998618408576964 6.192006377766569E-4 52-53 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.5581061748493601 6.57900677637698E-4 54-55 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.6629058827930593 6.96600717498739E-4 56-57 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.7778450011803513 7.095007307857527E-4 58-59 2.5800026574027372E-5 2.5800026574027372E-5 0.0 0.9134757408800132 7.224007440727664E-4 60-61 2.5800026574027372E-5 2.5800026574027372E-5 0.0 1.0560595877413754 7.740007972208211E-4 62-63 5.1600053148054744E-5 2.5800026574027372E-5 0.0 1.2066414428406862 7.998008237948486E-4 64-65 5.1600053148054744E-5 2.5800026574027372E-5 0.0 1.3635185044240594 7.998008237948486E-4 66-67 5.1600053148054744E-5 5.1600053148054744E-5 0.0 1.5428028890869756 7.998008237948486E-4 68-69 5.1600053148054744E-5 5.1600053148054744E-5 0.0 1.7534085060107611 7.998008237948486E-4 70-71 5.1600053148054744E-5 5.1600053148054744E-5 0.0 1.9879823476218181 7.998008237948486E-4 72-73 5.1600053148054744E-5 5.1600053148054744E-5 0.0 2.215409581871869 8.256008503688759E-4 74-75 5.1600053148054744E-5 5.1600053148054744E-5 0.0 2.4509896245193135 8.256008503688759E-4 76-77 5.1600053148054744E-5 7.740007972208211E-5 0.0 2.7171426986569793 8.514008769429033E-4 78-79 5.1600053148054744E-5 1.0320010629610949E-4 0.0 3.0166939071947247 8.772009035169306E-4 80-81 5.1600053148054744E-5 1.0320010629610949E-4 0.0 3.31727711679543 9.288009566649853E-4 82-83 5.1600053148054744E-5 1.0320010629610949E-4 0.0 3.63819074733647 0.0010062010363870674 84-85 5.1600053148054744E-5 1.0320010629610949E-4 0.0 3.97897039833951 0.0010062010363870674 86-87 5.1600053148054744E-5 1.0320010629610949E-4 0.0 4.33483016487507 0.001032001062961095 88 5.1600053148054744E-5 1.0320010629610949E-4 0.0 4.612270750638873 0.0010578010895351223 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6110 0.0 58.56026 2 GTACCTG 6635 0.0 55.074917 1 ACCTGGG 6810 0.0 51.685654 3 GGTATCA 8190 0.0 43.41063 1 TATAACG 520 0.0 38.87986 2 CCTGGGG 6200 0.0 37.213203 4 TAACGCA 545 0.0 37.074837 4 GTATCAA 13375 0.0 35.735947 1 CTGGGGG 4240 0.0 30.143879 5 ATAACGC 700 0.0 29.5386 3 TCAACGC 16335 0.0 28.680273 4 ATCAACG 16440 0.0 28.556107 3 CAACGCA 16470 0.0 28.530046 5 GTACATG 14220 0.0 28.313892 1 TATCAAC 16750 0.0 28.014011 2 AACGCAG 17135 0.0 27.828049 6 CATGGGG 9130 0.0 27.27799 4 TACATGG 14680 0.0 26.935776 2 ATGGGGG 6210 0.0 26.558992 5 TGGGGGG 7385 0.0 26.526892 6 >>END_MODULE