FastQCFastQC Report
Mon 6 Jun 2016
ERR1211491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875965
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181930.46937988346127996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160230.41339382579564055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116370.3002349092419565No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82370.21251481889026347No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49320.127245731063103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG60150.056.603942
GTACCTG66350.052.025431
ACCTGGG67100.049.6539233
TATAACG3950.046.431342
GGTATCA100050.040.4712521
CCTGGGG60400.036.6435054
TAACGCA5050.035.3594324
GTATCAA151700.032.5199931
ATAACGC5500.030.7588183
TATCACG4400.026.7196672
TGGGGGG62000.026.2228976
CTGGGGG38300.026.132945
TCAACGC189100.025.6200624
CAACGCA190400.025.4448055
GTACATG149750.025.3435381
CATGGGG92400.025.224514
ATCAACG190700.025.184313
TATCAAC195050.024.712782
AACGCAG197250.024.6320046
TACATGG155500.023.8913362
Produced by FastQC (version 0.11.5)