##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3875965 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.114353973784596 33.0 31.0 34.0 30.0 34.0 2 32.32203618969727 34.0 31.0 34.0 30.0 34.0 3 32.33393645195454 34.0 31.0 34.0 30.0 34.0 4 35.97907127644341 37.0 35.0 37.0 35.0 37.0 5 35.907979819219214 37.0 35.0 37.0 35.0 37.0 6 35.92915519102985 37.0 35.0 37.0 35.0 37.0 7 35.857457949181686 37.0 35.0 37.0 35.0 37.0 8 35.84685852426428 37.0 35.0 37.0 35.0 37.0 9 37.497326730246535 39.0 37.0 39.0 35.0 39.0 10-11 37.45478596942955 39.0 37.0 39.0 34.5 39.0 12-13 37.356695558396424 39.0 37.0 39.0 34.0 39.0 14-15 38.57705010236161 40.0 38.0 41.0 34.0 41.0 16-17 38.497335244255304 40.0 38.0 41.0 34.0 41.0 18-19 38.48953034921626 40.0 38.0 41.0 34.0 41.0 20-21 38.42637807616942 40.0 38.0 41.0 34.0 41.0 22-23 38.3014214524641 40.0 38.0 41.0 34.0 41.0 24-25 38.18565505622471 40.0 37.5 41.0 33.5 41.0 26-27 38.00231929338887 40.0 37.0 41.0 33.0 41.0 28-29 38.02317358386879 40.0 37.0 41.0 33.0 41.0 30-31 37.92081829944284 40.0 37.0 41.0 33.0 41.0 32-33 37.70075632777902 40.0 37.0 41.0 33.0 41.0 34-35 37.55489381354063 40.0 36.0 41.0 32.0 41.0 36-37 37.48285626934196 40.0 36.0 41.0 32.0 41.0 38-39 37.37680435710848 40.0 36.0 41.0 31.5 41.0 40-41 37.2043915515233 40.0 36.0 41.0 31.0 41.0 42-43 37.04911099558433 39.0 36.0 41.0 31.0 41.0 44-45 36.85464084428007 39.0 35.0 41.0 30.0 41.0 46-47 36.580251498659045 39.0 35.0 41.0 30.0 41.0 48-49 36.339211267387604 39.0 35.0 41.0 29.0 41.0 50-51 36.1291769920523 39.0 35.0 40.0 28.0 41.0 52-53 35.924856261601946 39.0 35.0 40.0 28.0 41.0 54-55 35.71706633057832 38.0 34.5 40.0 27.0 41.0 56-57 35.50758881981648 38.0 34.0 40.0 27.0 41.0 58-59 35.238006664146866 38.0 34.0 40.0 26.0 41.0 60-61 34.89863775859689 37.5 34.0 40.0 26.0 41.0 62-63 34.53877047393359 37.0 33.0 40.0 26.0 41.0 64-65 34.2169132074206 36.5 33.0 39.0 25.0 41.0 66-67 33.87211533127879 36.0 32.5 39.0 24.5 40.5 68-69 33.46816134820619 35.5 32.0 39.0 23.5 40.0 70-71 33.076030098311 35.0 31.5 38.0 23.0 40.0 72-73 32.66701131202165 35.0 31.0 37.0 22.0 39.5 74-75 32.26322490012165 35.0 31.0 37.0 21.5 39.0 76-77 30.7269305837385 33.5 29.5 35.0 20.0 37.5 78-79 31.575469463733548 34.0 30.5 36.0 21.0 38.0 80-81 31.56312299001668 34.0 31.0 35.5 20.5 37.0 82-83 31.287833739468752 34.0 30.0 35.0 20.0 37.0 84-85 31.002799818883815 34.0 30.0 35.0 20.0 36.5 86-87 30.717863035398928 34.0 30.0 35.0 19.5 36.0 88-89 30.453850073465574 34.0 29.5 35.0 19.0 36.0 90-91 30.176150455434968 34.0 29.0 35.0 18.0 35.0 92-93 29.963494381399215 34.0 29.0 35.0 18.0 35.0 94-95 29.749425368908128 34.0 29.0 35.0 17.5 35.0 96-97 29.55058314510064 34.0 29.0 35.0 17.0 35.0 98-99 29.38447264100682 33.0 28.0 35.0 15.5 35.0 100 27.524953398702 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27696319794343083 1101 2 0.1490830586497367 1101 3 0.21836996775311945 1101 4 0.13062695410341263 1101 5 0.10951342119540897 1101 6 0.13755961766781155 1101 7 0.14932227602985648 1101 8 0.1018591668007005 1101 9 0.19629390534782232 1101 10-11 0.09743437957813228 1101 12-13 0.16963868367351154 1101 14-15 0.27507059406617884 1101 16-17 0.3024883829005063 1101 18-19 0.22149970924031948 1101 20-21 0.16514481673183212 1101 22-23 0.15902589716654347 1101 24-25 0.17629192674366578 1101 26-27 0.11671391734389402 1101 28-29 0.24215973390403178 1101 30-31 0.28324514483985297 1101 32-33 0.505000234990419 1101 34-35 0.3832227794511027 1101 36-37 0.41428021855514174 1101 38-39 0.4165905125025091 1101 40-41 0.3365335595182799 1101 42-43 0.3951321295287187 1101 44-45 0.44227188151105423 1101 46-47 0.4358315479925565 1101 48-49 0.3984436723676339 1101 50-51 0.4272143908997279 1101 52-53 0.4345412952316181 1101 54-55 0.37838657194588876 1101 56-57 0.33204261484615216 1101 58-59 0.22919599484195885 1101 60-61 0.2751399756592079 1101 62-63 0.23444717095252798 1101 64-65 0.35731581552811775 1101 66-67 0.18701121101985763 1101 68-69 0.15200520129659623 1101 70-71 0.1672777031058672 1101 72-73 0.07366131988582936 1101 74-75 0.09308148841780195 1101 76-77 0.15113749352760664 1101 78-79 0.16133447237084297 1101 80-81 0.27125607917283645 1101 82-83 0.2534187128817713 1101 84-85 0.11876506093794248 1101 86-87 0.0625235338632173 1101 88-89 0.1592728691193983 1101 90-91 0.026587918916138875 1101 92-93 0.005493732014610231 1101 94-95 0.028655848683843743 1101 96-97 -0.03551258491391707 1101 98-99 -0.2306420474140971 1101 100 -0.25834407463848663 1102 1 0.2846721753407948 1102 2 0.21020592059867482 1102 3 0.2930425887383805 1102 4 0.19485857295838827 1102 5 0.1842252456865623 1102 6 0.14246362477440755 1102 7 0.08847410899112873 1102 8 0.1272749889247109 1102 9 0.19579799451681623 1102 10-11 0.15507442910108438 1102 12-13 0.16216565730777432 1102 14-15 0.3071562843863802 1102 16-17 0.2506535614922143 1102 18-19 0.20553755571008736 1102 20-21 0.2598426696342315 1102 22-23 0.22135598376465282 1102 24-25 0.22267765009215168 1102 26-27 0.16693428430600932 1102 28-29 0.2687691000055068 1102 30-31 0.3196154539105649 1102 32-33 0.4032443721466734 1102 34-35 0.4050894946688288 1102 36-37 0.40934979639664704 1102 38-39 0.3923974342086183 1102 40-41 0.35906757247999366 1102 42-43 0.40171007226282995 1102 44-45 0.45422335416777315 1102 46-47 0.4529144892007153 1102 48-49 0.3804478031734533 1102 50-51 0.37938054411762323 1102 52-53 0.3376186568886581 1102 54-55 0.39971338729532846 1102 56-57 0.3467559609740718 1102 58-59 0.40087879965435036 1102 60-61 0.3828176226961446 1102 62-63 0.41988326858904657 1102 64-65 0.3646202397816438 1102 66-67 0.31516379474178535 1102 68-69 0.16339238680065904 1102 70-71 0.25582236298273386 1102 72-73 0.26948983434224516 1102 74-75 0.28523804029155286 1102 76-77 0.3337480457919888 1102 78-79 0.23727896323464037 1102 80-81 0.25524074405910824 1102 82-83 0.2705327194887275 1102 84-85 0.24867204210360327 1102 86-87 0.17368185741099396 1102 88-89 0.14252096772232647 1102 90-91 0.08965805955777384 1102 92-93 0.10591891972099532 1102 94-95 0.0487047649259047 1102 96-97 0.1972386836033877 1102 98-99 0.10944771725888458 1102 100 0.0408185817348361 1103 1 0.28909380995277445 1103 2 0.3370499405326228 1103 3 0.3455338599642346 1103 4 0.18358434898713227 1103 5 0.1918456602767762 1103 6 0.17304030224531886 1103 7 0.19325244888333515 1103 8 0.18861070973613892 1103 9 0.3049548858220348 1103 10-11 0.25668025206100964 1103 12-13 0.2752398737247148 1103 14-15 0.3904269601906236 1103 16-17 0.3447189393613783 1103 18-19 0.33507988229726493 1103 20-21 0.3125077491079722 1103 22-23 0.31629512431975826 1103 24-25 0.39577502520399577 1103 26-27 0.3587386204824412 1103 28-29 0.40230016358214726 1103 30-31 0.5071613404683717 1103 32-33 0.4716486884930262 1103 34-35 0.5096125813616723 1103 36-37 0.5999188841423688 1103 38-39 0.5983389614548997 1103 40-41 0.639455982273212 1103 42-43 0.6039287389735577 1103 44-45 0.5771653936910042 1103 46-47 0.6410175064410737 1103 48-49 0.6746777234518362 1103 50-51 0.7222761836257447 1103 52-53 0.7253768905011171 1103 54-55 0.6722116698691778 1103 56-57 0.7925150987435146 1103 58-59 0.7944503142235533 1103 60-61 0.7352399608036464 1103 62-63 0.7444998281182222 1103 64-65 0.8305417244876949 1103 66-67 0.7942826075812874 1103 68-69 0.6922038334754674 1103 70-71 0.7869166290220804 1103 72-73 0.7199910749023033 1103 74-75 0.6278167527058898 1103 76-77 0.7110202419019913 1103 78-79 0.7140270197692438 1103 80-81 0.7082750406462139 1103 82-83 0.6757806662496968 1103 84-85 0.5823853535136863 1103 86-87 0.5404650651145069 1103 88-89 0.6745884419137198 1103 90-91 0.657719185764396 1103 92-93 0.651367858457867 1103 94-95 0.7532570065506476 1103 96-97 0.6171287211152787 1103 98-99 0.6592855355729021 1103 100 0.6662544302613895 1104 1 0.2666141233687256 1104 2 0.2371546078449569 1104 3 0.30955964741250597 1104 4 0.043907382953136675 1104 5 0.09022598200036924 1104 6 0.20173932031218555 1104 7 0.14753939754737644 1104 8 0.1624680456270724 1104 9 0.16087840051600466 1104 10-11 0.23081220315009432 1104 12-13 0.23111067094993842 1104 14-15 0.33089919670220524 1104 16-17 0.29782720499774484 1104 18-19 0.27696652222462603 1104 20-21 0.2701877901834919 1104 22-23 0.36023133964229714 1104 24-25 0.31174628834524754 1104 26-27 0.3845864786893145 1104 28-29 0.4294179278771182 1104 30-31 0.297172153171509 1104 32-33 0.35331497242827936 1104 34-35 0.46243114958721776 1104 36-37 0.435052082243935 1104 38-39 0.4913005523029028 1104 40-41 0.6392356414173719 1104 42-43 0.5957685862010251 1104 44-45 0.531477452347076 1104 46-47 0.45940701588101973 1104 48-49 0.5498665754586725 1104 50-51 0.49183986764700904 1104 52-53 0.441761152195987 1104 54-55 0.48928116135318334 1104 56-57 0.5365489213892403 1104 58-59 0.6248268311211547 1104 60-61 0.573188262005921 1104 62-63 0.6046160627849062 1104 64-65 0.691825884618197 1104 66-67 0.5824101979107681 1104 68-69 0.6027488246348014 1104 70-71 0.5547899970347743 1104 72-73 0.7143209467867493 1104 74-75 0.7016201818496555 1104 76-77 0.5768077128754854 1104 78-79 0.5697593849866927 1104 80-81 0.6691703499807744 1104 82-83 0.4961136728558131 1104 84-85 0.6229234490205293 1104 86-87 0.632698958122262 1104 88-89 0.6823998387904169 1104 90-91 0.5289932575704306 1104 92-93 0.5363487694581117 1104 94-95 0.5665986507777951 1104 96-97 0.5270068244021644 1104 98-99 0.5238298862517503 1104 100 0.6374579994805281 1105 1 0.18093926251375336 1105 2 0.14620940759588308 1105 3 0.1374454634532043 1105 4 0.09458687921867437 1105 5 0.07510465070780725 1105 6 0.09998527566004611 1105 7 0.1325598416600755 1105 8 0.15656128851530582 1105 9 0.15516023612535434 1105 10-11 0.20392963421283383 1105 12-13 0.2233140162539442 1105 14-15 0.32738392763994284 1105 16-17 0.2831973728055601 1105 18-19 0.28391475782391495 1105 20-21 0.3078769701889499 1105 22-23 0.3214925749709394 1105 24-25 0.3528580126584231 1105 26-27 0.36571471644585074 1105 28-29 0.42815716899323775 1105 30-31 0.4548599038426673 1105 32-33 0.464977992968997 1105 34-35 0.5580094443516899 1105 36-37 0.5693527638908193 1105 38-39 0.5114270351545329 1105 40-41 0.5934221682210321 1105 42-43 0.5539641145021719 1105 44-45 0.6084496689997465 1105 46-47 0.6289938919451643 1105 48-49 0.7811240984884904 1105 50-51 0.7995517217913815 1105 52-53 0.7086518637066987 1105 54-55 0.7359241282012263 1105 56-57 0.685636926693661 1105 58-59 0.7894608322050587 1105 60-61 0.7493877117122096 1105 62-63 0.6645226233632044 1105 64-65 0.7095559991700782 1105 66-67 0.8151037552525437 1105 68-69 0.7146728972052827 1105 70-71 0.7481062213687864 1105 72-73 0.7751181943917871 1105 74-75 0.6990846023439303 1105 76-77 0.6899381171322574 1105 78-79 0.7618495084851524 1105 80-81 0.8161327995156711 1105 82-83 0.7258162985297041 1105 84-85 0.8095275922371776 1105 86-87 0.6783233423008532 1105 88-89 0.6688506098037799 1105 90-91 0.8046646972872651 1105 92-93 0.7192035570332216 1105 94-95 0.8398576752703626 1105 96-97 0.7941080484020091 1105 98-99 0.8315869861059717 1105 100 0.9814739524395506 1106 1 0.20481769360366542 1106 2 0.17677916824536055 1106 3 0.17078695158008372 1106 4 0.09199398196489739 1106 5 0.09709349319069815 1106 6 0.11982640719799775 1106 7 0.11935646392879562 1106 8 0.1044282460475543 1106 9 0.0275078838847449 1106 10-11 0.1070524633374248 1106 12-13 0.08313858555283105 1106 14-15 0.20733620235925798 1106 16-17 0.24360889549500087 1106 18-19 0.2257965706153442 1106 20-21 0.3294766271236682 1106 22-23 0.23380299716930608 1106 24-25 0.2937494353608443 1106 26-27 0.21113705185013742 1106 28-29 0.3351550759295563 1106 30-31 0.3969347021355958 1106 32-33 0.41213109589050134 1106 34-35 0.4615108002694086 1106 36-37 0.44936092657974314 1106 38-39 0.4292844652260541 1106 40-41 0.4357348259381766 1106 42-43 0.4923065156726736 1106 44-45 0.47279298648481216 1106 46-47 0.5462215813379956 1106 48-49 0.554328913177855 1106 50-51 0.5269058149958212 1106 52-53 0.6120301645793305 1106 54-55 0.4435218241506007 1106 56-57 0.4253889848767045 1106 58-59 0.579741113474384 1106 60-61 0.6701536284198468 1106 62-63 0.6087378575777862 1106 64-65 0.6871805371356103 1106 66-67 0.6036417812353179 1106 68-69 0.6262677165314869 1106 70-71 0.6381466411725967 1106 72-73 0.6032384659791177 1106 74-75 0.6182002166756533 1106 76-77 0.5422953543874591 1106 78-79 0.5957921391177798 1106 80-81 0.5411015310144194 1106 82-83 0.6468430445606685 1106 84-85 0.5885701055558279 1106 86-87 0.5401409296076203 1106 88-89 0.5627496399247462 1106 90-91 0.701832214906073 1106 92-93 0.6003955182791181 1106 94-95 0.6670398918199467 1106 96-97 0.7326467624102406 1106 98-99 0.746788384128589 1106 100 0.7835450444182896 1107 1 0.21309671013210618 1107 2 0.20832772190631488 1107 3 0.26007081848546676 1107 4 0.20628303873919407 1107 5 0.11085461700089638 1107 6 0.156190815134309 1107 7 0.18104857911291816 1107 8 0.19861192267686079 1107 9 0.11433031925797366 1107 10-11 0.1746770287934254 1107 12-13 0.20254542654556928 1107 14-15 0.25154723332528306 1107 16-17 0.24666007964531644 1107 18-19 0.21112128436970323 1107 20-21 0.29726600675942194 1107 22-23 0.27810337416596553 1107 24-25 0.26310294399896605 1107 26-27 0.24413538535742418 1107 28-29 0.33820562926707254 1107 30-31 0.22008272178484134 1107 32-33 0.32318161711391724 1107 34-35 0.3323100744906071 1107 36-37 0.39080572873964314 1107 38-39 0.40019367291370855 1107 40-41 0.4289601832362777 1107 42-43 0.35323804266400316 1107 44-45 0.5095113950932273 1107 46-47 0.47949655492987375 1107 48-49 0.5099175131289968 1107 50-51 0.41046654678454075 1107 52-53 0.41323891659076395 1107 54-55 0.5028660541059509 1107 56-57 0.5978163295791958 1107 58-59 0.5424713171351527 1107 60-61 0.5447741371761126 1107 62-63 0.5057326654150032 1107 64-65 0.5836708667754351 1107 66-67 0.6110236099272086 1107 68-69 0.5542594035657586 1107 70-71 0.6358680880048269 1107 72-73 0.6482581416037121 1107 74-75 0.7025635717280707 1107 76-77 0.5243421928110692 1107 78-79 0.7289687632600241 1107 80-81 0.6571523905828656 1107 82-83 0.6373790175205905 1107 84-85 0.6378130728533478 1107 86-87 0.6304885189278444 1107 88-89 0.6318303507016658 1107 90-91 0.5547737523230225 1107 92-93 0.534626230604399 1107 94-95 0.7594998438093583 1107 96-97 0.7591157247002513 1107 98-99 0.7790121942149391 1107 100 1.0637338524229598 1108 1 0.21166313832434014 1108 2 0.14842358404970923 1108 3 0.2154484841539812 1108 4 0.08777556238587891 1108 5 0.1289409932178458 1108 6 0.14386717738724997 1108 7 0.132544994365837 1108 8 0.10951310749108956 1108 9 0.09136897042942849 1108 10-11 0.11265768849154512 1108 12-13 0.18464158519229557 1108 14-15 0.17855121976897692 1108 16-17 0.23481716972673894 1108 18-19 0.2830775741101377 1108 20-21 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80-81 -0.18464073035851314 2316 82-83 -0.04770976866281629 2316 84-85 -0.07559025210009906 2316 86-87 -0.09634641382600151 2316 88-89 -0.061576857004212115 2316 90-91 0.05290644885414153 2316 92-93 0.16682435960306563 2316 94-95 0.06990827816455081 2316 96-97 0.14684767149036304 2316 98-99 0.0246081880464466 2316 100 -0.20724881328303368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 20.0 12 53.0 13 138.0 14 442.0 15 1271.0 16 2873.0 17 6117.0 18 11223.0 19 16676.0 20 22124.0 21 28400.0 22 36080.0 23 41142.0 24 43895.0 25 46875.0 26 53257.0 27 62361.0 28 74708.0 29 89670.0 30 106866.0 31 129284.0 32 157414.0 33 198781.0 34 265320.0 35 357617.0 36 515796.0 37 784845.0 38 718743.0 39 103954.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22112316042676 18.94873641291377 13.158004051260233 23.672136375399237 2 15.88251604971293 21.04248649335266 39.569506758874525 23.505490698059887 3 17.488587926693377 27.805158956320003 30.725096550797108 23.98115656618951 4 12.09156608228264 17.058886208817334 37.347885012460225 33.5016626964398 5 12.187159030014003 37.55520988593333 34.45888958177616 15.798741502276508 6 29.800125904941773 36.73542418097288 19.336831839502157 14.127618074583198 7 26.785458589022348 33.0582448499922 22.739704821895966 17.41659173908949 8 26.936831434821723 34.89344882558539 20.518631523626194 17.65108821596669 9 27.7651959615724 15.322562335460288 20.379059248228312 36.533182454739 10-11 25.168261323309167 25.80154877559524 29.068051440092983 19.962138461002617 12-13 25.390425352138113 23.96714108615532 28.960774929598177 21.68165863210839 14-15 22.86240974828204 25.176981732291182 26.906589713787405 25.054018805639373 16-17 22.31697929879878 27.57936182874091 27.558321741505175 22.54533713095513 18-19 22.059868250889625 26.76701254086925 29.51445815227452 21.658661055966604 20-21 22.83298293621732 26.262639032746577 29.360044614839026 21.54433341619708 22-23 22.522429898102793 26.005626985795793 29.39980108179511 22.072142034306292 24-25 22.068545777444555 26.178838841274217 29.437382617926456 22.31523276335477 26-27 22.127776827475817 26.139388399418877 29.332841584319347 22.399993188785956 28-29 22.256169573469535 26.65741556911472 29.266746243133756 21.81966861428199 30-31 22.955343625287288 26.292922845433896 29.429633004704964 21.322100524573855 32-33 22.701298929150056 26.339807316930635 29.22091986873061 21.737973885188694 34-35 22.837492328994138 26.425052611779954 29.173378729605314 21.56407632962059 36-37 22.586460229390994 26.57184174575552 29.216804290083758 21.624893734769728 38-39 22.800503160101897 26.787225223744603 28.75145111326946 21.660820502884036 40-41 22.73162320433165 26.458191774728533 28.984025158482208 21.826159862457605 42-43 22.297907562655528 26.701465582112938 28.846977271563258 22.153649583668273 44-45 22.185025072598947 26.838620703890477 28.79357388063456 22.182780342876015 46-47 22.207526581807514 26.468317664242917 28.624211976385027 22.69994377756454 48-49 22.16393171909178 26.66247807601776 28.772843999295596 22.400746205594864 50-51 22.252065406673342 27.068767418140382 28.476216376748138 22.20295079843814 52-53 22.446697771646857 27.037634428715933 28.024256672720043 22.491411126917168 54-55 22.127713097042605 26.885219466572064 28.653026735898347 22.334040700486987 56-57 22.37350122649645 26.949664946841068 28.108667005084655 22.568166821577822 58-59 22.43369444177836 27.065730715684122 28.337505327871813 22.163069514665708 60-61 22.780975239211855 27.208368467279243 28.244712362642183 21.765943930866715 62-63 22.824862110118378 27.225223365480762 28.10491887881818 21.844995645582685 64-65 22.478295156968016 26.756180102432904 28.184734760296926 22.58078998030216 66-67 22.243422091533894 26.999059229817306 28.155592447829314 22.601926230819487 68-69 21.99637461456089 26.929156614070628 28.34923686281593 22.725231908552555 70-71 22.026555781706588 26.76425864195596 28.429700377619334 22.779485198718124 72-73 21.865985764159497 27.386879665438524 27.802539993066954 22.944594577335025 74-75 22.075157536594272 27.21692595847796 27.737264036969485 22.97065246795828 76-77 21.818726449517712 26.923349319936186 28.164045069345445 23.09387916120066 78-79 21.81247839077731 27.043780930215753 28.123648617267772 23.020092061739163 80-81 22.061745734599207 27.160166053043415 27.72470202215081 23.053386190206567 82-83 22.153515342412415 27.163915130094434 27.881610793639076 22.800958733854074 84-85 21.95276842179282 27.47603875087333 27.55097551330328 23.02021731403058 86-87 21.846726302609397 27.38269367144009 27.626057142739175 23.14452288321134 88-89 21.516405948899173 27.20437015424233 27.85956555741027 23.419658339448223 90-91 22.10868957643602 27.107271511553936 27.78491039610591 22.999128515904133 92-93 21.909930826800654 27.211673809729053 27.662388743985865 23.216006619484432 94-95 21.687842199190886 26.95178797564937 27.61980028543189 23.74056953972785 96-97 21.404323661870094 27.089124961887347 27.701301578531545 23.80524979771101 98-99 21.445943557262684 26.933492118583164 27.600222436975113 24.020341887179036 100 20.498394792941063 27.451040604087375 27.955659590302538 24.09490501266902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3013.0 1 2211.5 2 3799.0 3 5514.5 4 3509.5 5 2309.5 6 3066.5 7 5431.5 8 8202.5 9 9274.0 10 7713.0 11 5882.5 12 5766.5 13 6068.0 14 6352.5 15 6541.5 16 6837.5 17 7103.5 18 7427.0 19 7806.0 20 8278.0 21 9096.5 22 10257.0 23 12082.0 24 14335.5 25 17098.5 26 20807.5 27 25319.0 28 30129.5 29 35366.0 30 41599.5 31 47996.0 32 54866.5 33 62509.0 34 69931.0 35 76192.5 36 84753.5 37 93888.0 38 100072.5 39 105923.0 40 109306.0 41 111856.0 42 115571.0 43 119256.0 44 123275.0 45 128702.0 46 133862.0 47 138708.0 48 145099.0 49 150770.0 50 155783.0 51 156207.0 52 150465.0 53 144152.0 54 137791.5 55 128107.5 56 117512.0 57 109357.0 58 99559.5 59 85504.0 60 67468.5 61 51270.0 62 40330.0 63 30922.0 64 23053.0 65 17288.0 66 12902.5 67 9663.0 68 7324.0 69 5545.5 70 4209.0 71 3157.0 72 2418.5 73 1930.5 74 1473.0 75 1041.0 76 755.0 77 508.5 78 314.5 79 210.0 80 154.5 81 108.5 82 68.0 83 49.0 84 34.0 85 22.5 86 18.5 87 17.0 88 9.5 89 8.5 90 7.5 91 2.5 92 2.0 93 2.5 94 2.0 95 0.5 96 1.0 97 1.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034572035609196678 2 0.06932467140441155 3 6.708006909247116E-4 4 8.256008503688759E-4 5 0.0019092019664780253 6 6.450006643506843E-4 7 0.0 8 8.772009035169306E-4 9 2.5800026574027372E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.869008105078348E-4 18-19 9.675009965260263E-4 20-21 0.003212103308466408 22-23 0.0 24-25 1.2900013287013686E-5 26-27 1.0320010629610949E-4 28-29 7.353007573597801E-4 30-31 0.0014706015147195601 32-33 0.0030573031490222437 34-35 0.004373104504297639 36-37 0.0012900013287013687 38-39 0.0015867016343026831 40-41 0.005534105700128871 42-43 0.006953107161700376 44-45 0.005676005846286022 46-47 0.007430407653319883 48-49 0.009223509500214786 50-51 0.008668808928873197 52-53 0.00426990439800153 54-55 0.0023220023916624634 56-57 0.002257502325227395 58-59 0.0031218032154573115 60-61 0.008281808530262787 62-63 0.01522201567867615 64-65 0.004115104238557365 66-67 0.010758611081369414 68-69 0.013287013685624096 70-71 0.006101706284757474 72-73 0.008372108623271882 74-75 0.0030831031755962713 76-77 0.0029799030693001614 78-79 0.007869008105078349 80-81 0.005559905726702898 82-83 0.01665391715353467 84-85 0.017995518535384093 86-87 0.023142623836902552 88-89 0.022226722893524578 90-91 0.02492282567051044 92-93 0.03464943568891876 94-95 0.03832593947571766 96-97 0.024239124966298715 98-99 0.018318018867559436 100 0.01971122030255691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3875965.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.759030910675776 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42521385635088 47.963723587657306 2 15.209156670038043 19.090238671604563 3 4.202719798048664 7.912758651439354 4 1.5628480424525242 3.9233131441986826 5 0.7726238022686558 2.4244560544451206 6 0.4538808145556304 1.7091072042275706 7 0.2778960079759696 1.220833890816209 8 0.19844120362620463 0.9963182105862944 9 0.1492589342537442 0.843061146167273 >10 0.6940433202580529 7.466042070964712 >50 0.034369556651277715 1.4998711710579968 >100 0.017638739506104287 2.0639875693652976 >500 0.0012238807802539104 0.507096792060057 >1k 4.8955230994128E-4 0.717339949032038 >5k 4.8955230994128E-5 0.2530721757828035 >10k+ 1.46865692982384E-4 1.4087797105947422 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18193 0.46937988346127996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16023 0.41339382579564055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11637 0.3002349092419565 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8237 0.21251481889026347 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4932 0.127245731063103 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.5800026574027372E-5 5 0.0 0.0 0.0 0.0 2.5800026574027372E-5 6 0.0 0.0 0.0 0.0 2.5800026574027372E-5 7 0.0 0.0 0.0 0.0 2.5800026574027372E-5 8 0.0 0.0 0.0 0.0 2.5800026574027372E-5 9 0.0 0.0 0.0 0.0 5.1600053148054744E-5 10-11 0.0 0.0 0.0 0.0 5.1600053148054744E-5 12-13 0.0 0.0 0.0 0.0 1.677001727311779E-4 14-15 0.0 0.0 0.0 0.0 2.0640021259221898E-4 16-17 0.0 0.0 0.0 1.2900013287013686E-5 2.838002923143011E-4 18-19 0.0 0.0 0.0 7.740007972208211E-5 3.0960031888832844E-4 20-21 0.0 0.0 0.0 9.03000930090958E-5 3.0960031888832844E-4 22-23 0.0 0.0 0.0 1.677001727311779E-4 3.4830035874936947E-4 24-25 0.0 0.0 0.0 2.709002790272874E-4 3.8700039861041055E-4 26-27 0.0 0.0 0.0 5.805005979156159E-4 3.8700039861041055E-4 28-29 0.0 0.0 0.0 0.0017673018203208748 3.8700039861041055E-4 30-31 0.0 0.0 0.0 0.006179106364479556 3.999004118974243E-4 32-33 0.0 5.1600053148054744E-5 0.0 0.01546711593112941 4.386004517584653E-4 34-35 0.0 5.1600053148054744E-5 0.0 0.030082830985315917 4.386004517584653E-4 36-37 0.0 6.450006643506843E-5 0.0 0.0526965542774509 4.386004517584653E-4 38-39 0.0 9.03000930090958E-5 0.0 0.09156429431122315 4.386004517584653E-4 40-41 0.0 1.0320010629610949E-4 0.0 0.14143574567881806 4.9020050490652E-4 42-43 0.0 1.0320010629610949E-4 0.0 0.18848209413655695 4.9020050490652E-4 44-45 0.0 1.0320010629610949E-4 0.0 0.24341035071266123 5.031005181935337E-4 46-47 1.2900013287013686E-5 1.0320010629610949E-4 0.0 0.3097293190211986 5.160005314805475E-4 48-49 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.3838269953418052 6.450006643506842E-4 50-51 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.46759968162767207 6.450006643506842E-4 52-53 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.555152071806634 7.482007706467937E-4 54-55 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.6594873792720006 8.772009035169306E-4 56-57 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.7736395968487848 9.03000930090958E-4 58-59 2.5800026574027372E-5 1.0320010629610949E-4 0.0 0.9081351353791894 9.288009566649853E-4 60-61 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.0492483807258322 9.546009832390128E-4 62-63 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.1975211334467675 9.546009832390128E-4 64-65 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.3517794923328772 9.546009832390128E-4 66-67 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.5285096743649644 9.8040100981304E-4 68-69 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.7356580877278303 0.0010062010363870674 70-71 2.5800026574027372E-5 1.0320010629610949E-4 0.0 1.9653170242765348 0.0010578010895351223 72-73 2.5800026574027372E-5 1.0320010629610949E-4 0.0 2.1885904542481676 0.0010836011161091496 74-75 2.5800026574027372E-5 1.0320010629610949E-4 0.0 2.4212292938661726 0.001109401142683177 76-77 2.5800026574027372E-5 1.0320010629610949E-4 0.0 2.6842863648149557 0.001109401142683177 78-79 2.5800026574027372E-5 1.0320010629610949E-4 0.0 2.979412868795255 0.001109401142683177 80-81 2.5800026574027372E-5 1.1610011958312318E-4 0.0 3.274461972695832 0.0011739012091182455 82-83 2.5800026574027372E-5 1.2900013287013687E-4 0.0 3.5899317976297516 0.0012900013287013685 84-85 2.5800026574027372E-5 1.2900013287013687E-4 0.0 3.924867742613775 0.0012900013287013685 86-87 2.5800026574027372E-5 1.2900013287013687E-4 0.0 4.273774401987634 0.0013029013419883822 88 2.5800026574027372E-5 1.2900013287013687E-4 0.0 4.545577681945012 0.001315801355275396 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6015 0.0 56.60394 2 GTACCTG 6635 0.0 52.02543 1 ACCTGGG 6710 0.0 49.653923 3 TATAACG 395 0.0 46.43134 2 GGTATCA 10005 0.0 40.471252 1 CCTGGGG 6040 0.0 36.643505 4 TAACGCA 505 0.0 35.359432 4 GTATCAA 15170 0.0 32.519993 1 ATAACGC 550 0.0 30.758818 3 TATCACG 440 0.0 26.719667 2 TGGGGGG 6200 0.0 26.222897 6 CTGGGGG 3830 0.0 26.13294 5 TCAACGC 18910 0.0 25.620062 4 CAACGCA 19040 0.0 25.444805 5 GTACATG 14975 0.0 25.343538 1 CATGGGG 9240 0.0 25.22451 4 ATCAACG 19070 0.0 25.18431 3 TATCAAC 19505 0.0 24.71278 2 AACGCAG 19725 0.0 24.632004 6 TACATGG 15550 0.0 23.891336 2 >>END_MODULE