FastQCFastQC Report
Mon 6 Jun 2016
ERR1211490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5308875
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97040.18278825551552824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80670.15195309740764285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.11305596760142216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66150.043.2738341
GTATCAA115050.036.380341
GTACATG179400.029.9968321
TCAACGC134650.029.8735984
ATCAACG135650.029.586043
CAACGCA135400.029.5667935
AACGCAG141700.028.3455896
TACCTGG45350.028.3055482
TACATGG183850.028.1841012
ACATGGG183000.027.452093
TATCAAC149650.027.1785092
GTACCTG50950.026.3361511
CATGGGG106100.024.2708684
ACGCAGA163400.024.2373547
CGCAGAG167800.023.4902638
ACCTGGG58550.020.7081643
ATGGGGG61650.019.8925655
GCAGAGT194450.019.7170079
CATGGGA83000.019.5326484
AGAGTAC174750.018.51131810-11
Produced by FastQC (version 0.11.5)