FastQCFastQC Report
Mon 6 Jun 2016
ERR1211490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5308875
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124620.23473899837536202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110190.20755809846718937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82740.1558522285795013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.046.4812551
GTATCAA132500.036.7989851
TCAACGC161050.029.4986114
ATCAACG163250.029.0147253
CAACGCA164400.028.954685
AACGCAG170550.028.075636
TATCAAC175150.027.2200242
TACCTGG42700.027.0874392
GTACATG195900.026.4736651
TACATGG197400.025.7715662
GTACCTG46400.025.2321551
ACATGGG196400.025.074423
ACGCAGA198150.023.9276497
CGCAGAG202350.023.1987848
CATGGGG113000.022.4141064
GCAGAGT232600.019.9191199
ACCTGGG56200.019.899943
AGAGTAC208650.018.9744310-11
GAGTACT123500.018.70253212-13
ATGGGGG66550.018.1466395
Produced by FastQC (version 0.11.5)