##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5308875 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14522398813308 33.0 31.0 34.0 30.0 34.0 2 32.39412417885145 34.0 31.0 34.0 31.0 34.0 3 32.41788439170257 34.0 31.0 34.0 31.0 34.0 4 36.033166725530364 37.0 35.0 37.0 35.0 37.0 5 35.952335287608015 37.0 35.0 37.0 35.0 37.0 6 35.970613171340446 37.0 35.0 37.0 35.0 37.0 7 35.898549692731514 37.0 35.0 37.0 35.0 37.0 8 35.89628537119446 37.0 35.0 37.0 35.0 37.0 9 37.57485079230534 39.0 37.0 39.0 35.0 39.0 10-11 37.53821930258294 39.0 37.0 39.0 35.0 39.0 12-13 37.45360890960891 39.0 37.0 39.0 34.5 39.0 14-15 38.71196854324127 40.0 38.0 41.0 34.0 41.0 16-17 38.643141720232634 40.0 38.0 41.0 34.0 41.0 18-19 38.62301071319253 40.0 38.0 41.0 34.0 41.0 20-21 38.559682606955334 40.0 38.0 41.0 34.0 41.0 22-23 38.455325563325566 40.0 38.0 41.0 34.0 41.0 24-25 38.347812954722045 40.0 38.0 41.0 34.0 41.0 26-27 38.176071201525744 40.0 38.0 41.0 34.0 41.0 28-29 38.22469798215253 40.0 38.0 41.0 34.0 41.0 30-31 38.172366273457186 40.0 38.0 41.0 34.0 41.0 32-33 38.02396863742318 40.0 37.5 41.0 33.0 41.0 34-35 37.930559958559954 40.0 37.0 41.0 33.0 41.0 36-37 37.8767853829672 40.0 37.0 41.0 33.0 41.0 38-39 37.78929368274823 40.0 37.0 41.0 33.0 41.0 40-41 37.64119563937746 40.0 37.0 41.0 32.5 41.0 42-43 37.49823851569306 40.0 37.0 41.0 32.0 41.0 44-45 37.32600014127287 40.0 36.5 41.0 31.0 41.0 46-47 37.09258496385769 39.5 36.0 41.0 31.0 41.0 48-49 36.885783334510606 39.0 36.0 41.0 30.0 41.0 50-51 36.699686185868 39.0 35.5 41.0 30.0 41.0 52-53 36.50047816156907 39.0 35.0 40.0 29.5 41.0 54-55 36.29875048856867 39.0 35.0 40.0 29.0 41.0 56-57 36.09364601728238 38.5 35.0 40.0 28.5 41.0 58-59 35.82773516046243 38.0 34.5 40.0 28.0 41.0 60-61 35.50864627628264 38.0 34.0 40.0 28.0 41.0 62-63 35.182499776317954 37.5 34.0 40.0 27.0 41.0 64-65 34.86064853664854 37.0 33.5 40.0 26.0 41.0 66-67 34.51054488945398 36.5 33.0 39.0 26.0 41.0 68-69 34.103010807374446 36.0 33.0 39.0 26.0 40.0 70-71 33.70952744225471 35.5 32.0 38.5 25.5 40.0 72-73 33.29065517647336 35.0 32.0 38.0 25.0 40.0 74-75 32.87227727155 35.0 31.0 37.0 24.5 39.0 76-77 31.37474361328907 33.5 29.5 35.5 22.5 38.5 78-79 32.139724423724424 34.0 31.0 36.0 24.0 38.5 80-81 32.07816119234301 34.0 31.0 36.0 24.0 37.5 82-83 31.788981658072565 34.0 31.0 35.5 23.0 37.0 84-85 31.483863530408982 34.0 31.0 35.0 22.5 37.0 86-87 31.1795246638883 34.0 30.0 35.0 21.0 36.0 88-89 30.898548562548562 34.0 30.0 35.0 20.0 36.0 90-91 30.61815949706859 34.0 29.5 35.0 20.0 35.5 92-93 30.406457488639305 34.0 29.0 35.0 19.5 35.0 94-95 30.1846847966848 34.0 29.0 35.0 19.0 35.0 96-97 29.98091610746156 34.0 29.0 35.0 18.0 35.0 98-99 29.803631183631182 34.0 29.0 35.0 18.0 35.0 100 27.751423606696335 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2591306823459192 1101 2 0.1520099585672483 1101 3 0.22151015235521498 1101 4 0.10766067385895894 1101 5 0.11531221676115422 1101 6 0.12783301940978475 1101 7 0.14075832145646672 1101 8 0.13663688097004467 1101 9 0.13718050508283142 1101 10-11 0.1478511578093844 1101 12-13 0.1608233774124983 1101 14-15 0.2023517144826883 1101 16-17 0.16041172908750667 1101 18-19 0.24403915371519247 1101 20-21 0.1934238871483629 1101 22-23 0.16241758997627187 1101 24-25 0.17594222410145477 1101 26-27 0.18068522730618497 1101 28-29 0.23288769065976567 1101 30-31 0.22838607577287462 1101 32-33 0.2971991089554251 1101 34-35 0.25315418895426944 1101 36-37 0.2763589758067937 1101 38-39 0.25818229701366135 1101 40-41 0.27787612566854847 1101 42-43 0.215128540123942 1101 44-45 0.31216433066687443 1101 46-47 0.347073765648787 1101 48-49 0.3776182222739308 1101 50-51 0.24678415685031752 1101 52-53 0.27084502978400593 1101 54-55 0.26385174152265023 1101 56-57 0.2913597134656314 1101 58-59 0.3103948378267489 1101 60-61 0.28246447168547206 1101 62-63 0.23069624533334832 1101 64-65 0.331676343945567 1101 66-67 0.30992425753458974 1101 68-69 0.258608164657673 1101 70-71 0.22921213360775283 1101 72-73 0.07425903737608763 1101 74-75 0.25572666386641885 1101 76-77 0.20250130595141158 1101 78-79 0.3436385757618723 1101 80-81 0.3131322816991613 1101 82-83 0.25172884396323525 1101 84-85 0.23967075087409384 1101 86-87 0.2221863045364607 1101 88-89 0.22900421780536817 1101 90-91 0.1979665663198027 1101 92-93 0.18414115925239116 1101 94-95 0.23096790940015666 1101 96-97 0.057257469935255045 1101 98-99 0.03905470755664808 1101 100 0.29017538373101814 1102 1 0.27936818077951386 1102 2 0.22789414201774605 1102 3 0.3194170506057148 1102 4 0.21587629046236145 1102 5 0.17110718907807865 1102 6 0.18189259349077957 1102 7 0.16994591045969543 1102 8 0.18202463241905775 1102 9 0.23350632792729442 1102 10-11 0.2315006839936089 1102 12-13 0.2818063071333867 1102 14-15 0.31846815645980797 1102 16-17 0.30484897795027166 1102 18-19 0.38068506705125316 1102 20-21 0.3759008632692087 1102 22-23 0.32841358478530935 1102 24-25 0.29501065707317053 1102 26-27 0.3052221671164759 1102 28-29 0.3756659857739706 1102 30-31 0.39242659225150334 1102 32-33 0.43366842468840616 1102 34-35 0.3777229403418616 1102 36-37 0.41315809489953637 1102 38-39 0.40397805695056377 1102 40-41 0.419993113444292 1102 42-43 0.3323448657186958 1102 44-45 0.3887433169792871 1102 46-47 0.4096913209310742 1102 48-49 0.44542991787701425 1102 50-51 0.4302732012965649 1102 52-53 0.4158011508149855 1102 54-55 0.4366978459815911 1102 56-57 0.4163999030919783 1102 58-59 0.4239504144862778 1102 60-61 0.40622342836176983 1102 62-63 0.47840631195025907 1102 64-65 0.4835884464900886 1102 66-67 0.4721556322729086 1102 68-69 0.44958920235666255 1102 70-71 0.40957624078516375 1102 72-73 0.4274491416947157 1102 74-75 0.3852652572785118 1102 76-77 0.38736551753347825 1102 78-79 0.42904077313490774 1102 80-81 0.41760041780282364 1102 82-83 0.4331543374144857 1102 84-85 0.3675096757201395 1102 86-87 0.4276116490001556 1102 88-89 0.40361678180449445 1102 90-91 0.3307349518275693 1102 92-93 0.3225112834195798 1102 94-95 0.4071187498960427 1102 96-97 0.3314269993659984 1102 98-99 0.3386915553934706 1102 100 0.5602000880550797 1103 1 0.23828966427140585 1103 2 0.19221652441011372 1103 3 0.2751803847417875 1103 4 0.1345880069463803 1103 5 0.09022922155642732 1103 6 0.13816131155290634 1103 7 0.15053331223477784 1103 8 0.16098214102168384 1103 9 0.24212333060847158 1103 10-11 0.19478955692409983 1103 12-13 0.22602072156594488 1103 14-15 0.27750848320271615 1103 16-17 0.3261254878481097 1103 18-19 0.3254666712364056 1103 20-21 0.22837409002553244 1103 22-23 0.26542386072506474 1103 24-25 0.31140884158578075 1103 26-27 0.2630349137687418 1103 28-29 0.2827770303868107 1103 30-31 0.3477147367835727 1103 32-33 0.35852334355520554 1103 34-35 0.3710995965529449 1103 36-37 0.3924600455227605 1103 38-39 0.351690227666559 1103 40-41 0.38724536211266525 1103 42-43 0.515202313639243 1103 44-45 0.5591211731604204 1103 46-47 0.594030608142333 1103 48-49 0.5438852823993372 1103 50-51 0.538281759211074 1103 52-53 0.4906901054018533 1103 54-55 0.6234976286491687 1103 56-57 0.5870992929565944 1103 58-59 0.7100628570078626 1103 60-61 0.7104430773660368 1103 62-63 0.6799769535590983 1103 64-65 0.6664236696556358 1103 66-67 0.5844539513745062 1103 68-69 0.6264613553622098 1103 70-71 0.6197506802695116 1103 72-73 0.549360475959638 1103 74-75 0.4925396480051063 1103 76-77 0.460247525058751 1103 78-79 0.6623401618924518 1103 80-81 0.5829589139381923 1103 82-83 0.5332670217574105 1103 84-85 0.5752480286313748 1103 86-87 0.4429535490956624 1103 88-89 0.4691370101329255 1103 90-91 0.5636987684592363 1103 92-93 0.5886966738226604 1103 94-95 0.5481940252245749 1103 96-97 0.5909166362945335 1103 98-99 0.6582911516732111 1103 100 0.8910606659147149 1104 1 0.19515143007262736 1104 2 0.15703278612858185 1104 3 0.2145859887864887 1104 4 0.03148672226800642 1104 5 0.09034792501179822 1104 6 0.12681827541732105 1104 7 0.14679272076274685 1104 8 0.1330387677546483 1104 9 0.14508960033941776 1104 10-11 0.20357490880855522 1104 12-13 0.18481054870710523 1104 14-15 0.24343912477375085 1104 16-17 0.22939428083930835 1104 18-19 0.27749505746223946 1104 20-21 0.2097445548457486 1104 22-23 0.26058144194978183 1104 24-25 0.24730060163533807 1104 26-27 0.28226743180263014 1104 28-29 0.3374342922378304 1104 30-31 0.29911732043931494 1104 32-33 0.28920458473217536 1104 34-35 0.338818123644991 1104 36-37 0.265586262019994 1104 38-39 0.32293789717321886 1104 40-41 0.3894326097185825 1104 42-43 0.3969522167833617 1104 44-45 0.3679103215088304 1104 46-47 0.3757385011785175 1104 48-49 0.3706539280686414 1104 50-51 0.36015288109584986 1104 52-53 0.4507888257444961 1104 54-55 0.5072871505041476 1104 56-57 0.4761567581861996 1104 58-59 0.45409466557712364 1104 60-61 0.4173386293087873 1104 62-63 0.45365600752257507 1104 64-65 0.5608773837332635 1104 66-67 0.5240494638927018 1104 68-69 0.6366294490058664 1104 70-71 0.4630927671867724 1104 72-73 0.6081975150124563 1104 74-75 0.6763720565998668 1104 76-77 0.5169946499280336 1104 78-79 0.6617898792793113 1104 80-81 0.7207160646786761 1104 82-83 0.5439274277491464 1104 84-85 0.5859426471420086 1104 86-87 0.6748324433279471 1104 88-89 0.736553501740417 1104 90-91 0.5582901205577677 1104 92-93 0.6202367788424752 1104 94-95 0.6877189194198863 1104 96-97 0.5756578869800606 1104 98-99 0.6281154882965616 1104 100 0.8370393714699063 1105 1 0.17754426646972377 1105 2 0.16685240393454137 1105 3 0.17914528728121581 1105 4 0.1146818750645835 1105 5 0.06258104236403028 1105 6 0.10976422755808102 1105 7 0.11676853885956717 1105 8 0.15265658046341457 1105 9 0.07697293262578597 1105 10-11 0.1487279752829167 1105 12-13 0.20117461040047857 1105 14-15 0.25011922455657043 1105 16-17 0.2524484621615457 1105 18-19 0.2776793787715164 1105 20-21 0.2835400124750649 1105 22-23 0.3361520316976794 1105 24-25 0.31497195972662695 1105 26-27 0.33381913484920744 1105 28-29 0.3561759250759877 1105 30-31 0.4112982684678954 1105 32-33 0.4106441235304743 1105 34-35 0.43780585267354155 1105 36-37 0.41481068207838945 1105 38-39 0.4866510820828296 1105 40-41 0.4647160313536389 1105 42-43 0.4681415866780867 1105 44-45 0.5449855109058461 1105 46-47 0.5762855679040086 1105 48-49 0.5567725008350237 1105 50-51 0.6591448339786865 1105 52-53 0.6419095283185641 1105 54-55 0.681031415829132 1105 56-57 0.6343384651590753 1105 58-59 0.6650209095916111 1105 60-61 0.6951681781653889 1105 62-63 0.6805915870647326 1105 64-65 0.6319025226160804 1105 66-67 0.6508399884515441 1105 68-69 0.6866737724864436 1105 70-71 0.7204798565105577 1105 72-73 0.6582573237096412 1105 74-75 0.7383573544831421 1105 76-77 0.6409613490877923 1105 78-79 0.8606074935883825 1105 80-81 0.8103555472443844 1105 82-83 0.8241164816644897 1105 84-85 0.7978304421395954 1105 86-87 0.7538662224893287 1105 88-89 0.879628823250961 1105 90-91 0.90718257670677 1105 92-93 0.8196070365792281 1105 94-95 0.8839028155098347 1105 96-97 0.9819265287012016 1105 98-99 0.9178933493219041 1105 100 1.2723763456396888 1106 1 0.21483035923058225 1106 2 0.17302888004911665 1106 3 0.2080340187206673 1106 4 0.16450777165213992 1106 5 0.11074530370783009 1106 6 0.10133972578116612 1106 7 0.13483605752776384 1106 8 0.08689140751346969 1106 9 0.08299641747071718 1106 10-11 0.14897520786983165 1106 12-13 0.1665903304132712 1106 14-15 0.20927068604400034 1106 16-17 0.2477022423833617 1106 18-19 0.2776308064899595 1106 20-21 0.20970554498384786 1106 22-23 0.23849375717301768 1106 24-25 0.23132602184382023 1106 26-27 0.26534966994938713 1106 28-29 0.26867463981884043 1106 30-31 0.3114755752369902 1106 32-33 0.39732412947005713 1106 34-35 0.37789867955553547 1106 36-37 0.4026962526986395 1106 38-39 0.4142659866815208 1106 40-41 0.3538584268850684 1106 42-43 0.3617903743087254 1106 44-45 0.4261228747534034 1106 46-47 0.4343312972228546 1106 48-49 0.539802409095067 1106 50-51 0.5361272941983444 1106 52-53 0.5743436252903678 1106 54-55 0.4756615462233569 1106 56-57 0.5834603866093815 1106 58-59 0.5984856262165508 1106 60-61 0.6439037069527984 1106 62-63 0.6864379739133 1106 64-65 0.6792943248415 1106 66-67 0.6487803801822665 1106 68-69 0.5703227049531563 1106 70-71 0.6833044132990906 1106 72-73 0.6035161992910147 1106 74-75 0.5847021222991557 1106 76-77 0.5681231842314425 1106 78-79 0.689740189016625 1106 80-81 0.7140986923221249 1106 82-83 0.6999165379287433 1106 84-85 0.691957776023056 1106 86-87 0.6764924047301797 1106 88-89 0.767252592861901 1106 90-91 0.7184326151992408 1106 92-93 0.7042325687539801 1106 94-95 0.8536018996238468 1106 96-97 0.8287050218735494 1106 98-99 0.8143136602471372 1106 100 1.0186933988751683 1107 1 0.18991721892391666 1107 2 0.16518334265330736 1107 3 0.2307652850573234 1107 4 0.13658901936593537 1107 5 0.14186810462725674 1107 6 0.1504023152586953 1107 7 0.1438658705121867 1107 8 0.0960031200759417 1107 9 0.17586470731267667 1107 10-11 0.18231411973076916 1107 12-13 0.14308362951540943 1107 14-15 0.2380456670174098 1107 16-17 0.25034547436371923 1107 18-19 0.18792047273699808 1107 20-21 0.28342460161862704 1107 22-23 0.29245116016431894 1107 24-25 0.3491104158856331 1107 26-27 0.3306220155251012 1107 28-29 0.35958454925764727 1107 30-31 0.3138537871038878 1107 32-33 0.2620619937854016 1107 34-35 0.2540572734102753 1107 36-37 0.34455992053342754 1107 38-39 0.35212327198863136 1107 40-41 0.35723992460405896 1107 42-43 0.3448403177992958 1107 44-45 0.36505147126635507 1107 46-47 0.41716937842468127 1107 48-49 0.5297868212598829 1107 50-51 0.47895800828320745 1107 52-53 0.4533509049965261 1107 54-55 0.4405215645924514 1107 56-57 0.5349729045561489 1107 58-59 0.6964294789931458 1107 60-61 0.7267926485616059 1107 62-63 0.6944911921053176 1107 64-65 0.6714766961047189 1107 66-67 0.6748024330855529 1107 68-69 0.6961843167112178 1107 70-71 0.7608585394357235 1107 72-73 0.7047272876992352 1107 74-75 0.7578850282807466 1107 76-77 0.6813161392528109 1107 78-79 0.8624009050829571 1107 80-81 0.8288035853284654 1107 82-83 0.8051371810313981 1107 84-85 0.6784099164217388 1107 86-87 0.6624924633031171 1107 88-89 0.5134906594269033 1107 90-91 0.5521940788972195 1107 92-93 0.6895037178781145 1107 94-95 0.8082446091899982 1107 96-97 0.9113687076006478 1107 98-99 0.9294780368016582 1107 100 0.9700764828057018 1108 1 0.22744584989015237 1108 2 0.14952386405416718 1108 3 0.186614484950141 1108 4 0.13252718815415676 1108 5 0.12637401018970706 1108 6 0.15320590427535308 1108 7 0.14921853348986502 1108 8 0.11213460956518873 1108 9 0.14584211894329258 1108 10-11 0.1606606755177964 1108 12-13 0.15485632719433085 1108 14-15 0.18275703305420166 1108 16-17 0.21786930263971982 1108 18-19 0.3370646025816981 1108 20-21 0.2782405342990728 1108 22-23 0.32240888579575255 1108 24-25 0.32968083019368777 1108 26-27 0.314902072198052 1108 28-29 0.3307178465188585 1108 30-31 0.31438330434824735 1108 32-33 0.29469231734320545 1108 34-35 0.31299220573770725 1108 36-37 0.4498216079840702 1108 38-39 0.39526887367959773 1108 40-41 0.39965134338216046 1108 42-43 0.42435722518951025 1108 44-45 0.4239734310462069 1108 46-47 0.4227798786957919 1108 48-49 0.42110025468412005 1108 50-51 0.4869064622133976 1108 52-53 0.42322690927341 1108 54-55 0.42937629525182786 1108 56-57 0.5211775177649329 1108 58-59 0.5782931550467438 1108 60-61 0.6426061935655767 1108 62-63 0.5386429445873375 1108 64-65 0.5346912908662205 1108 66-67 0.5285860648502307 1108 68-69 0.5507251655708174 1108 70-71 0.6648609279032129 1108 72-73 0.6560261925029778 1108 74-75 0.6145465722676633 1108 76-77 0.5813618848852613 1108 78-79 0.6521519944842851 1108 80-81 0.5927057820648685 1108 82-83 0.6278171840676094 1108 84-85 0.6022182052025435 1108 86-87 0.6997211911173302 1108 88-89 0.6095048505080349 1108 90-91 0.6181273842457138 1108 92-93 0.7633214212480297 1108 94-95 0.8361500461523868 1108 96-97 0.8930530524219726 1108 98-99 0.9995592035006986 1108 100 1.2111789367276096 1109 1 0.02305132323343173 1109 2 0.009246658946956643 1109 3 0.09118161751888465 1109 4 0.011108770198248408 1109 5 0.03505996718433124 1109 6 0.025355943805500658 1109 7 0.018240984876094046 1109 8 0.012401054677312118 1109 9 0.05697346690158156 1109 10-11 0.010730409980283184 1109 12-13 0.08454601195946054 1109 14-15 0.056478190523847616 1109 16-17 0.11912687472234751 1109 18-19 0.07566999203623936 1109 20-21 0.06803729057219243 1109 22-23 0.12396585258649395 1109 24-25 0.10481073748945136 1109 26-27 0.15976605158103752 1109 28-29 0.17144132657585942 1109 30-31 0.2039907113174806 1109 32-33 0.17236150789686633 1109 34-35 0.15702288827656474 1109 36-37 0.19909863763076885 1109 38-39 0.15430918093598223 1109 40-41 0.0874489243009009 1109 42-43 0.1553893139734015 1109 44-45 0.102193704373569 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-0.4698555661192785 2316 76-77 -0.38386226073315655 2316 78-79 -0.3146621056393535 2316 80-81 -0.20248835579714353 2316 82-83 -0.132928169802625 2316 84-85 -0.3517407727645079 2316 86-87 -0.2516741746219431 2316 88-89 -0.26610902441234785 2316 90-91 -0.12521376874741819 2316 92-93 0.02995573091349968 2316 94-95 -0.09653156289023102 2316 96-97 -0.09432842332687486 2316 98-99 -0.11583997789603373 2316 100 -0.49744402600260784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 7.0 11 13.0 12 31.0 13 110.0 14 389.0 15 933.0 16 2194.0 17 4237.0 18 7585.0 19 12005.0 20 17879.0 21 25339.0 22 34915.0 23 42669.0 24 47681.0 25 54818.0 26 64681.0 27 78503.0 28 94951.0 29 116591.0 30 141487.0 31 170914.0 32 212347.0 33 268094.0 34 355379.0 35 489737.0 36 728698.0 37 1116769.0 38 1063181.0 39 156723.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.35744120870406 21.22795173351487 14.026877420322212 23.387729637458854 2 15.316558981619059 21.492916422970303 39.98881525588542 23.201709339525216 3 17.55166953798141 26.88117579114359 30.529735908110894 25.0374187627641 4 11.976553783686088 17.26526988709944 38.65457386004565 32.10360246916883 5 12.486046747305133 38.905379334455745 35.66506015109258 12.943513767146536 6 31.29620588120059 38.37745966733272 17.477369855004323 12.848964596462359 7 27.496089096089094 32.6525111252384 22.579906288997197 17.271493489675308 8 25.958425907002198 36.936088614545376 19.843826376220015 17.26165910223241 9 26.643828172193984 15.047336788806213 19.937433048407826 38.37140199059198 10-11 24.75698712062348 26.593487320760044 29.02433189705917 19.625193661557297 12-13 25.29573591391773 24.09501071319253 28.97431188340279 21.634941489486945 14-15 22.51157731157731 25.66562595653505 26.060474205928752 25.762322525958893 16-17 22.26841460806235 28.702179602287547 26.819318473891308 22.210087315758795 18-19 22.21126455198177 27.90499495838335 28.480255379121473 21.403485110513405 20-21 22.87047030238939 27.115887001746096 28.457326146516245 21.556316549348274 22-23 22.68103154444506 26.966987070423308 28.23646518673591 22.115516198395724 24-25 22.476815934080165 27.280020380975433 28.27906490502456 21.96409877991984 26-27 22.438410046900835 27.327584956547174 28.275603750032257 21.958401246519735 28-29 22.396178426134306 27.156531857672366 28.49929103664053 21.9479986795528 30-31 22.65112970365299 27.15340561198902 28.338013805296697 21.857450879061293 32-33 22.48532106972413 27.27317507844884 28.284766491021507 21.95673736080552 34-35 22.614878330878224 27.34934993011419 28.306399355016143 21.72937238399144 36-37 22.681986491316554 27.22462624610141 28.200702323450226 21.892684939131808 38-39 22.57742500492343 27.369215463724984 28.23669988604766 21.816659645303922 40-41 22.5644845278387 27.241462389605996 28.346763430426748 21.847289652128556 42-43 22.456490994913374 27.177903853752287 28.29349570102067 22.072109450313672 44-45 22.474818471562248 27.365690430637734 28.109266401229625 22.05022469657039 46-47 22.513192907872494 27.36330783602403 27.960751499378173 22.162747756725302 48-49 22.479605314528524 27.430758946690354 27.966162120077133 22.123473618703986 50-51 22.507370566392744 27.507431318641213 27.828118419978125 22.15707969498792 52-53 22.58813900306292 27.52341457166215 27.70128431656953 22.1871621087054 54-55 22.449800532008826 27.544101041554725 27.798492544332266 22.207605882104183 56-57 22.537436562626063 27.587674564329124 27.64911167662096 22.225777196423856 58-59 22.501539702762724 27.451588721774982 27.857953460712125 22.18891811475017 60-61 22.591879725026033 27.652544196786195 27.753212888192103 22.002363189995673 62-63 22.408187894226607 27.578012643886474 27.888561762623638 22.125237699263288 64-65 22.352719231349198 27.433531716038186 27.87073091730833 22.343018135304295 66-67 22.377591156303485 27.493014096515715 27.675603153775025 22.453791593405768 68-69 22.337798954007134 27.561612491329544 27.753878956147194 22.346709598516124 70-71 22.333599191349577 27.372622677399942 27.784944348771557 22.508833782478916 72-73 22.347366218034562 27.60838405223917 27.546445733235352 22.497803996490916 74-75 22.302382825983866 27.66719080455873 27.526591874591176 22.503834494866226 76-77 22.2420596162877 27.570531636928468 27.652255714516333 22.535153032267495 78-79 22.27134015538129 27.48063432368035 27.609184681973968 22.6388408389644 80-81 22.21169924357941 27.752579665567705 27.373413354856098 22.66230773599679 82-83 22.332534065438235 27.692536665296092 27.456430008953497 22.51849926031218 84-85 22.23837937698547 27.69449469416914 27.41839857089406 22.648727357951323 86-87 22.110985339767268 27.639621892151993 27.599208846330946 22.650183921749793 88-89 22.09073559859426 27.566575759685286 27.5824015061589 22.760287135561555 90-91 22.216148206568114 27.688433330387912 27.467583251643426 22.62783521140055 92-93 22.193857098375645 27.542438949480257 27.437402049015652 22.82630190312845 94-95 21.98728222436803 27.44370787800937 27.53419245949887 23.034817438123724 96-97 22.038849529994955 27.41342827950816 27.59053859207428 22.957183598422596 98-99 21.96019378708383 27.348749022817582 27.488632468351256 23.202424721747335 100 20.78306698882342 27.913055355385058 27.83281759993218 23.471060055859343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 672.0 1 525.5 2 553.0 3 802.5 4 722.5 5 678.5 6 1124.0 7 2315.5 8 3667.5 9 4374.5 10 4000.5 11 3405.5 12 3527.0 13 3794.0 14 4148.0 15 4428.5 16 4658.0 17 4923.0 18 5301.5 19 5690.0 20 6236.0 21 7350.5 22 8765.0 23 10845.0 24 13765.5 25 17105.0 26 21677.0 27 27567.0 28 34171.0 29 42873.5 30 53465.5 31 64231.5 32 77099.0 33 92435.5 34 108868.5 35 124434.0 36 143297.5 37 161282.5 38 172897.5 39 183140.0 40 187010.0 41 188038.5 42 191613.0 43 195257.5 44 199895.0 45 206162.0 46 209194.5 47 208929.0 48 211056.0 49 210957.0 50 206649.5 51 198608.5 52 187408.5 53 178056.0 54 168945.0 55 155787.0 56 139417.5 57 122406.5 58 106291.0 59 90293.5 60 74368.5 61 60388.5 62 47773.0 63 36321.5 64 26593.5 65 19271.5 66 14006.0 67 10169.5 68 7389.5 69 5402.0 70 4150.5 71 3140.5 72 2255.5 73 1615.5 74 1192.5 75 817.0 76 532.5 77 334.0 78 231.0 79 150.0 80 91.5 81 65.0 82 45.0 83 30.0 84 21.5 85 16.5 86 12.0 87 6.5 88 5.0 89 5.5 90 4.0 91 3.5 92 2.5 93 0.0 94 0.5 95 1.0 96 1.5 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034093852275670455 2 0.06468413741141013 3 7.722916813825906E-4 4 8.288008288008288E-4 5 0.0014504014504014503 6 5.085823267641449E-4 7 0.0 8 7.534552989098443E-4 9 3.767276494549222E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.440371076734714E-4 18-19 0.0011678557133102587 20-21 0.00326811235902145 22-23 9.418191236373054E-6 24-25 4.709095618186527E-5 26-27 2.637093546184455E-4 28-29 0.0012714558169103624 30-31 0.0021756021756021756 32-33 0.0030232393868757507 34-35 0.004049822231640413 36-37 0.0012714558169103624 38-39 0.0014409832591650773 40-41 0.005198841562477926 42-43 0.0065456429092792725 44-45 0.005114077841350569 46-47 0.006423206423206424 48-49 0.008391608391608392 50-51 0.008090226272044454 52-53 0.004049822231640413 54-55 0.0024204751477478748 56-57 0.0036636763909491184 58-59 0.004153422335240517 60-61 0.007016552471097926 62-63 0.013128958583504038 64-65 0.0037013491558946106 66-67 0.009540627722445905 68-69 0.01162204798568435 70-71 0.005217677944950673 72-73 0.006931788749970569 74-75 0.0029667302394575123 76-77 0.0029761484306938856 78-79 0.008466953921499376 80-81 0.007280261825716371 82-83 0.0168114713569259 84-85 0.01772503590685409 86-87 0.022283440465258646 88-89 0.020126674672129218 90-91 0.02264133173224082 92-93 0.03188057733512279 94-95 0.03566669021214476 96-97 0.021963221963221962 98-99 0.01666078029714393 100 0.016952744225471498 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5308875.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.485332262284466 #Duplication Level Percentage of deduplicated Percentage of total 1 75.32147593470656 46.311659743310955 2 15.169796060667018 18.654399022824116 3 4.6946555972796995 8.659573777672076 4 1.8932329429441435 4.656242261872932 5 0.9381116226343982 2.88400524083934 6 0.5454121365217544 2.012090786035351 7 0.3432464566480455 1.4773235704399827 8 0.21596766275721502 1.062307480202909 9 0.1497207689673607 0.8285068103870611 >10 0.6647413745003196 6.993640760165127 >50 0.03657036007459819 1.5646789235351684 >100 0.024488433944556125 2.8683879234732226 >500 0.0019354863378699726 0.8069216124744525 >1k 5.241941384578971E-4 0.4329762303846991 >5k 4.032262603521848E-5 0.20513314991998938 >10k+ 8.064525207043696E-5 0.5821527064625659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12462 0.23473899837536202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11019 0.20755809846718937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8274 0.1558522285795013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 1.883638247274611E-5 8 0.0 0.0 0.0 0.0 1.883638247274611E-5 9 0.0 0.0 0.0 1.883638247274611E-5 1.883638247274611E-5 10-11 1.3185467730922277E-4 0.0 0.0 1.883638247274611E-5 1.883638247274611E-5 12-13 1.69527442254715E-4 0.0 0.0 1.883638247274611E-5 3.767276494549222E-5 14-15 1.8836382472746111E-4 0.0 0.0 1.883638247274611E-5 5.6509147418238326E-5 16-17 2.1661839843658024E-4 0.0 0.0 5.6509147418238326E-5 5.6509147418238326E-5 18-19 2.260365896729533E-4 0.0 0.0 7.534552989098443E-5 5.6509147418238326E-5 20-21 2.6370935461844555E-4 0.0 0.0 9.418191236373054E-5 5.6509147418238326E-5 22-23 2.825457370911916E-4 0.0 0.0 1.5069105978196887E-4 5.6509147418238326E-5 24-25 3.673094582185491E-4 0.0 0.0 3.2021850203668386E-4 5.6509147418238326E-5 26-27 3.8614584069129523E-4 0.0 0.0 3.296366932730569E-4 5.6509147418238326E-5 28-29 3.955640319276683E-4 0.0 0.0 6.498551953097408E-4 5.6509147418238326E-5 30-31 4.332367968731605E-4 0.0 0.0 0.002910221092039274 5.6509147418238326E-5 32-33 4.6149137058227967E-4 0.0 0.0 0.0071954981045890135 5.6509147418238326E-5 34-35 5.650914741823832E-4 0.0 0.0 0.014127286854559582 7.534552989098443E-5 36-37 6.404370040733677E-4 9.418191236373054E-6 0.0 0.026041298768571496 7.534552989098443E-5 38-39 6.498551953097408E-4 1.883638247274611E-5 0.0 0.04561230015775471 7.534552989098443E-5 40-41 6.7810976901886E-4 1.883638247274611E-5 0.0 0.06731181276635823 7.534552989098443E-5 42-43 7.157825339643522E-4 3.767276494549222E-5 0.0 0.0897930352475807 7.534552989098443E-5 44-45 8.005462550917097E-4 3.767276494549222E-5 0.0 0.11786866332320878 7.534552989098443E-5 46-47 8.75891784982694E-4 5.6509147418238326E-5 0.0 0.1531868804596077 7.534552989098443E-5 48-49 9.041463586918132E-4 5.6509147418238326E-5 0.0 0.19025688116597206 7.534552989098443E-5 50-51 9.041463586918132E-4 5.6509147418238326E-5 0.0 0.23262932353841445 7.534552989098443E-5 52-53 9.229827411645593E-4 5.6509147418238326E-5 0.0 0.274879329424784 8.47637211273575E-5 54-55 9.229827411645593E-4 5.6509147418238326E-5 0.0 0.3229874502601775 9.418191236373054E-5 56-57 9.229827411645593E-4 5.6509147418238326E-5 0.0 0.3812295448659085 9.418191236373054E-5 58-59 9.229827411645593E-4 5.6509147418238326E-5 0.0 0.44775964775964777 9.418191236373054E-5 60-61 9.606555061100516E-4 5.6509147418238326E-5 0.0 0.5210520119611028 9.418191236373054E-5 62-63 9.794918885827977E-4 5.6509147418238326E-5 0.0 0.5979515434060889 9.418191236373054E-5 64-65 0.001036001036001036 5.6509147418238326E-5 0.0 0.6792964611146429 1.0360010360010359E-4 66-67 0.0010548374184737822 5.6509147418238326E-5 0.0 0.7743825198370653 1.1301829483647665E-4 68-69 0.0010548374184737822 5.6509147418238326E-5 0.0 0.8876174330719786 1.1301829483647665E-4 70-71 0.0010642556097101553 5.6509147418238326E-5 0.0 1.0131242494878858 1.224364860728497E-4 72-73 0.0010736738009465283 5.6509147418238326E-5 0.0 1.1428880883426338 1.69527442254715E-4 74-75 0.0010736738009465283 5.6509147418238326E-5 0.0 1.2736031645122554 1.69527442254715E-4 76-77 0.0010925101834192742 9.418191236373054E-5 0.0 1.4178145087235996 1.69527442254715E-4 78-79 0.0010925101834192742 9.418191236373054E-5 0.0 1.5882931882931883 1.69527442254715E-4 80-81 0.0010925101834192742 9.418191236373054E-5 0.0 1.7790021426385063 1.69527442254715E-4 82-83 0.0011396011396011395 9.418191236373054E-5 0.0 1.9817663817663815 1.69527442254715E-4 84-85 0.0011866920957830048 9.418191236373054E-5 0.0 2.1812813449177084 1.69527442254715E-4 86-87 0.0011866920957830048 9.418191236373054E-5 0.0 2.3906571542935176 1.7894563349108805E-4 88 0.0011866920957830048 9.418191236373054E-5 0.0 2.5631042358315086 1.883638247274611E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7870 0.0 46.481255 1 GTATCAA 13250 0.0 36.798985 1 TCAACGC 16105 0.0 29.498611 4 ATCAACG 16325 0.0 29.014725 3 CAACGCA 16440 0.0 28.95468 5 AACGCAG 17055 0.0 28.07563 6 TATCAAC 17515 0.0 27.220024 2 TACCTGG 4270 0.0 27.087439 2 GTACATG 19590 0.0 26.473665 1 TACATGG 19740 0.0 25.771566 2 GTACCTG 4640 0.0 25.232155 1 ACATGGG 19640 0.0 25.07442 3 ACGCAGA 19815 0.0 23.927649 7 CGCAGAG 20235 0.0 23.198784 8 CATGGGG 11300 0.0 22.414106 4 GCAGAGT 23260 0.0 19.919119 9 ACCTGGG 5620 0.0 19.89994 3 AGAGTAC 20865 0.0 18.97443 10-11 GAGTACT 12350 0.0 18.702532 12-13 ATGGGGG 6655 0.0 18.146639 5 >>END_MODULE