FastQCFastQC Report
Mon 6 Jun 2016
ERR1211489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9350642
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262530.2807614707097117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237870.2543889499779801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148870.15920831959987347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135550.049.70431
GTATCAA235050.037.316971
TCAACGC276550.030.7527774
ATCAACG279950.030.3460433
CAACGCA281450.030.1141515
AACGCAG292200.029.0819826
TATCAAC301900.028.3770222
GTACATG275900.027.5767771
TACATGG285450.026.1413752
ACATGGG279950.025.596083
ACGCAGA331600.025.3163577
GTACCTG83450.024.9937381
TACCTGG79400.024.990422
CGCAGAG337800.024.6442818
CATGGGG153750.021.8509524
GCAGAGT381600.021.301169
GAGTACT236900.019.824812-13
AGAGTAC346600.019.20847510-11
ACCTGGG98900.019.1466523
CAGAGTA373400.019.05035810-11
Produced by FastQC (version 0.11.5)