FastQCFastQC Report
Mon 6 Jun 2016
ERR1211489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9350642
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285980.30583996264641505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262490.2807186928983058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181010.19358029106450658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155600.048.2956051
GTATCAA250700.034.2145541
TCAACGC303950.027.5813054
ATCAACG305700.027.4387863
CAACGCA308400.027.1524165
AACGCAG318500.026.3206066
TATCAAC326550.025.964052
TACCTGG75550.024.4615822
GTACATG291250.024.1710911
TACATGG293700.023.424312
ACATGGG286800.023.0043563
ACGCAGA365200.022.8261857
GTACCTG84700.022.6525521
CGCAGAG373800.022.2255978
CATGGGG152500.021.2002354
TATAACG8150.020.1946742
GCAGAGT419250.019.4462979
GAGTACT259850.019.11541212-13
TAACGCA8800.018.689924
ACCTGGG97750.018.5563553
Produced by FastQC (version 0.11.5)