##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9350642 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12733318204247 33.0 31.0 34.0 30.0 34.0 2 32.348807921424005 34.0 31.0 34.0 31.0 34.0 3 32.36512658703007 34.0 31.0 34.0 30.0 34.0 4 35.99437824696957 37.0 35.0 37.0 35.0 37.0 5 35.91683063045297 37.0 35.0 37.0 35.0 37.0 6 35.9323124551234 37.0 35.0 37.0 35.0 37.0 7 35.859716798055146 37.0 35.0 37.0 35.0 37.0 8 35.85653551916542 37.0 35.0 37.0 35.0 37.0 9 37.518986824647975 39.0 37.0 39.0 35.0 39.0 10-11 37.48425942304283 39.0 37.0 39.0 34.5 39.0 12-13 37.4009145575245 39.0 37.0 39.0 34.0 39.0 14-15 38.64953721894176 40.0 38.0 41.0 34.0 41.0 16-17 38.580042525422314 40.0 38.0 41.0 34.0 41.0 18-19 38.5680898167206 40.0 38.0 41.0 34.0 41.0 20-21 38.511602572315354 40.0 38.0 41.0 34.0 41.0 22-23 38.40571192865688 40.0 38.0 41.0 34.0 41.0 24-25 38.302234274395275 40.0 38.0 41.0 34.0 41.0 26-27 38.131874902279435 40.0 38.0 41.0 33.0 41.0 28-29 38.17034407904826 40.0 38.0 41.0 33.5 41.0 30-31 38.1048670241038 40.0 37.5 41.0 33.0 41.0 32-33 37.93825851743655 40.0 37.0 41.0 33.0 41.0 34-35 37.83102315327653 40.0 37.0 41.0 33.0 41.0 36-37 37.782002401546336 40.0 37.0 41.0 33.0 41.0 38-39 37.68804714157595 40.0 37.0 41.0 33.0 41.0 40-41 37.538852626375814 40.0 37.0 41.0 32.0 41.0 42-43 37.39581431948737 40.0 37.0 41.0 31.5 41.0 44-45 37.22125079753882 40.0 36.0 41.0 31.0 41.0 46-47 36.97938585393388 39.5 36.0 41.0 30.5 41.0 48-49 36.77386771945713 39.0 36.0 41.0 30.0 41.0 50-51 36.58215558888898 39.0 35.5 41.0 30.0 41.0 52-53 36.38996536280611 39.0 35.0 40.5 29.0 41.0 54-55 36.204997475039676 39.0 35.0 40.0 28.5 41.0 56-57 36.01179362871555 38.5 35.0 40.0 28.0 41.0 58-59 35.76429933901865 38.0 34.5 40.0 28.0 41.0 60-61 35.46168632057564 38.0 34.0 40.0 27.0 41.0 62-63 35.15146660518069 38.0 34.0 40.0 26.5 41.0 64-65 34.84161991230121 37.0 34.0 40.0 26.0 41.0 66-67 34.50830648847426 36.5 33.0 39.5 26.0 41.0 68-69 34.11651060964584 36.0 33.0 39.0 26.0 40.5 70-71 33.731231449134725 36.0 32.0 39.0 25.0 40.0 72-73 33.3235505112911 35.0 32.0 38.0 24.5 40.0 74-75 32.91378816556125 35.0 31.5 37.5 24.0 39.5 76-77 31.349322912801068 33.5 29.5 36.0 21.5 38.5 78-79 32.1820364312953 34.5 31.0 36.0 23.5 39.0 80-81 32.14194303449966 35.0 31.0 36.0 23.5 38.5 82-83 31.84747828010098 34.0 31.0 36.0 23.0 37.0 84-85 31.54037583729545 34.0 31.0 35.0 22.0 37.0 86-87 31.237834418214277 34.0 30.5 35.0 20.5 36.5 88-89 30.950888559309618 34.0 30.0 35.0 20.0 36.0 90-91 30.66437946185941 34.0 30.0 35.0 20.0 36.0 92-93 30.454237848053644 34.0 29.0 35.0 19.0 35.0 94-95 30.233988104773985 34.0 29.0 35.0 19.0 35.0 96-97 30.025982226674916 34.0 29.0 35.0 18.0 35.0 98-99 29.863138434772715 34.0 29.0 35.0 18.0 35.0 100 27.97195123072833 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30115105709610646 1101 2 0.2015474441816849 1101 3 0.29243589027127825 1101 4 0.189245456265283 1101 5 0.1594676519336602 1101 6 0.16635702842640399 1101 7 0.21024037656113848 1101 8 0.18182246928407864 1101 9 0.21190170215970738 1101 10-11 0.2075072005465728 1101 12-13 0.19279624789005823 1101 14-15 0.2865940883075382 1101 16-17 0.30011556819749075 1101 18-19 0.3386832727593898 1101 20-21 0.3126028754419181 1101 22-23 0.27657218729303423 1101 24-25 0.3042339822420459 1101 26-27 0.24047520834612612 1101 28-29 0.3227873098724032 1101 30-31 0.3830320506413045 1101 32-33 0.4091694772593115 1101 34-35 0.3408958486199296 1101 36-37 0.3585942376544651 1101 38-39 0.39774662888197554 1101 40-41 0.3657037014652147 1101 42-43 0.35247185302768713 1101 44-45 0.38162094451902817 1101 46-47 0.3819012950903087 1101 48-49 0.4101671722507021 1101 50-51 0.35340014131179487 1101 52-53 0.3526715830049767 1101 54-55 0.28345282268372074 1101 56-57 0.36276555734269067 1101 58-59 0.43281070736025384 1101 60-61 0.38993687833480095 1101 62-63 0.32004566627416153 1101 64-65 0.25163409059950936 1101 66-67 0.20510719350360773 1101 68-69 0.20926501167244993 1101 70-71 0.2155670301101722 1101 72-73 0.10852185175229323 1101 74-75 0.22353753835916734 1101 76-77 0.24473802871343509 1101 78-79 0.3463473987017167 1101 80-81 0.2686984607148091 1101 82-83 0.2807199688331927 1101 84-85 0.2559599176385383 1101 86-87 0.24226210939964687 1101 88-89 0.23797143181389657 1101 90-91 0.17041051105458038 1101 92-93 0.057036166869071536 1101 94-95 0.11336921184799564 1101 96-97 0.09710343972241375 1101 98-99 -0.07301631877126624 1101 100 -0.011053420281395887 1102 1 0.306739915589084 1102 2 0.2508061620357438 1102 3 0.333237547032212 1102 4 0.21474995853380108 1102 5 0.20497979279506495 1102 6 0.1724503191586706 1102 7 0.13992499630622746 1102 8 0.1533460934394597 1102 9 0.21494643385614154 1102 10-11 0.21514984264327097 1102 12-13 0.20883443630604148 1102 14-15 0.33650325971935047 1102 16-17 0.3372097949915869 1102 18-19 0.27777249744276133 1102 20-21 0.33772493498095457 1102 22-23 0.2549176972512086 1102 24-25 0.29905517601748954 1102 26-27 0.282703200790948 1102 28-29 0.36773000919337306 1102 30-31 0.36936858765184866 1102 32-33 0.42130640038185163 1102 34-35 0.3969484128555081 1102 36-37 0.458136720830602 1102 38-39 0.44404402607763416 1102 40-41 0.4430468672889347 1102 42-43 0.3541185796720896 1102 44-45 0.41137412633464976 1102 46-47 0.4109988647602947 1102 48-49 0.45032716724077915 1102 50-51 0.445294477124051 1102 52-53 0.47539702362674063 1102 54-55 0.4107848684224322 1102 56-57 0.47028593535956986 1102 58-59 0.5201304296207709 1102 60-61 0.5223011404255757 1102 62-63 0.600574224064168 1102 64-65 0.5154320443540996 1102 66-67 0.5067524446809344 1102 68-69 0.5055588103370283 1102 70-71 0.5166100716202422 1102 72-73 0.5620078770683747 1102 74-75 0.46360105111511984 1102 76-77 0.5128523199460631 1102 78-79 0.5703446420189167 1102 80-81 0.5036929536448937 1102 82-83 0.4239334346113246 1102 84-85 0.3734333749704568 1102 86-87 0.3758638085608439 1102 88-89 0.3925425411313377 1102 90-91 0.4191763674374407 1102 92-93 0.3093079216118326 1102 94-95 0.3286974066176356 1102 96-97 0.3478899510357145 1102 98-99 0.31937961483038535 1102 100 0.32659296401027404 1103 1 0.28579146735995664 1103 2 0.22034687101102435 1103 3 0.28672280655069216 1103 4 0.1237681724194033 1103 5 0.14136333541585344 1103 6 0.15607963068072905 1103 7 0.15636702120060164 1103 8 0.18714460814732803 1103 9 0.1984894622840443 1103 10-11 0.20393202131178612 1103 12-13 0.2312106319553351 1103 14-15 0.3121109610629844 1103 16-17 0.2999096813289057 1103 18-19 0.29775111010572886 1103 20-21 0.25032559575998903 1103 22-23 0.2656049115233756 1103 24-25 0.26658963320510765 1103 26-27 0.2782806826698092 1103 28-29 0.3676403291270134 1103 30-31 0.36093424534983143 1103 32-33 0.38101729087093616 1103 34-35 0.3524521532616163 1103 36-37 0.43899002666204723 1103 38-39 0.39094731224061263 1103 40-41 0.4391841701881134 1103 42-43 0.459267098694113 1103 44-45 0.41545660753750724 1103 46-47 0.5394156243341541 1103 48-49 0.4894477183111974 1103 50-51 0.49665601755583566 1103 52-53 0.4441336692797364 1103 54-55 0.45563611161234263 1103 56-57 0.5494544109139454 1103 58-59 0.5381265693015607 1103 60-61 0.5064345626236957 1103 62-63 0.5413541281200835 1103 64-65 0.5318737295512506 1103 66-67 0.5155169726818443 1103 68-69 0.573552266409763 1103 70-71 0.6280127561794515 1103 72-73 0.5229990863836846 1103 74-75 0.5066648075277485 1103 76-77 0.5241132828246258 1103 78-79 0.5864092633006734 1103 80-81 0.5813378871627535 1103 82-83 0.559638737760519 1103 84-85 0.43851154949193827 1103 86-87 0.48032342161853236 1103 88-89 0.5712555506516921 1103 90-91 0.5410125124446701 1103 92-93 0.49927747794687605 1103 94-95 0.5159106597009 1103 96-97 0.5487658263505288 1103 98-99 0.5502962741935633 1103 100 0.604001943838373 1104 1 0.22578679845951655 1104 2 0.15747657934556258 1104 3 0.2577094539062159 1104 4 0.0527417010010538 1104 5 0.13822865690060837 1104 6 0.14181262593820065 1104 7 0.18012846222252676 1104 8 0.1468276573093945 1104 9 0.23159511578920444 1104 10-11 0.21969393185131025 1104 12-13 0.22388665058452517 1104 14-15 0.3164134105537144 1104 16-17 0.26595950122780465 1104 18-19 0.301930629031709 1104 20-21 0.22839119603168 1104 22-23 0.2594489971902263 1104 24-25 0.2590406122022202 1104 26-27 0.3380509674758798 1104 28-29 0.3617842517579746 1104 30-31 0.31947104818147665 1104 32-33 0.27418851141480616 1104 34-35 0.41727919383358625 1104 36-37 0.32958061203672884 1104 38-39 0.3439246411133041 1104 40-41 0.4727771651572965 1104 42-43 0.4958250267829456 1104 44-45 0.4250308418240465 1104 46-47 0.4380049028904125 1104 48-49 0.5157122909769996 1104 50-51 0.4781544864610652 1104 52-53 0.4423984951527089 1104 54-55 0.5159720833345602 1104 56-57 0.537926938833202 1104 58-59 0.5811154396028755 1104 60-61 0.5245135713660929 1104 62-63 0.5909444909485373 1104 64-65 0.6315464041397121 1104 66-67 0.6527423257512481 1104 68-69 0.6597440137026425 1104 70-71 0.5922306312550134 1104 72-73 0.6802647490578906 1104 74-75 0.7142245913157765 1104 76-77 0.5882343076371725 1104 78-79 0.6567807711802871 1104 80-81 0.7041542690524594 1104 82-83 0.5781421099919264 1104 84-85 0.6439283427637399 1104 86-87 0.732916398879496 1104 88-89 0.7136243224357379 1104 90-91 0.6007455304249163 1104 92-93 0.6011755501065927 1104 94-95 0.5484394083091999 1104 96-97 0.5663321248015869 1104 98-99 0.6927406343507485 1104 100 0.9129749147129047 1105 1 0.23636597728543762 1105 2 0.18119321784229214 1105 3 0.22135792612264282 1105 4 0.13847214351105208 1105 5 0.11394452274908673 1105 6 0.15000015293801283 1105 7 0.15235984883228326 1105 8 0.14994527123889156 1105 9 0.13162299612659467 1105 10-11 0.17428177290928915 1105 12-13 0.23102124356068288 1105 14-15 0.3138217194646984 1105 16-17 0.28422616049873994 1105 18-19 0.27535923951194974 1105 20-21 0.21859292185046542 1105 22-23 0.32882899538056876 1105 24-25 0.33435775432389647 1105 26-27 0.3412413127203209 1105 28-29 0.3959470473335571 1105 30-31 0.4149320718338245 1105 32-33 0.4475977644067939 1105 34-35 0.45579536955199274 1105 36-37 0.49585394915323633 1105 38-39 0.4484519159005771 1105 40-41 0.5017239376564078 1105 42-43 0.4527447546417278 1105 44-45 0.4949050414183631 1105 46-47 0.5003826543286252 1105 48-49 0.522678716243 1105 50-51 0.5494315879297744 1105 52-53 0.523205922708776 1105 54-55 0.45808380178676344 1105 56-57 0.5172579976303879 1105 58-59 0.504803880641667 1105 60-61 0.5441122025912719 1105 62-63 0.5106926341889917 1105 64-65 0.5853904286448355 1105 66-67 0.5923356098361978 1105 68-69 0.6332926702580508 1105 70-71 0.648509244581831 1105 72-73 0.575182719731103 1105 74-75 0.6265727266976455 1105 76-77 0.5349183413759455 1105 78-79 0.6880114026678612 1105 80-81 0.6818909433376561 1105 82-83 0.6714310643381616 1105 84-85 0.6962526599534833 1105 86-87 0.6870062574808884 1105 88-89 0.7323688298234998 1105 90-91 0.7516451732571134 1105 92-93 0.6887769381413307 1105 94-95 0.7012400762063464 1105 96-97 0.7010707793825937 1105 98-99 0.7184793291253797 1105 100 0.8437594157672663 1106 1 0.22783722446621368 1106 2 0.1778195954924584 1106 3 0.195480009011753 1106 4 0.1257830363674728 1106 5 0.1205884067209766 1106 6 0.11342698924526928 1106 7 0.14702010514346142 1106 8 0.14217012583188904 1106 9 0.1579581191184971 1106 10-11 0.17052379792999517 1106 12-13 0.2271868062075484 1106 14-15 0.2268768766415974 1106 16-17 0.2768663664915749 1106 18-19 0.2805698956666305 1106 20-21 0.29909377127913217 1106 22-23 0.33021706192916156 1106 24-25 0.326778172326776 1106 26-27 0.3207303777788937 1106 28-29 0.3286687276562432 1106 30-31 0.3309193007561433 1106 32-33 0.4144423356084772 1106 34-35 0.3801251980559499 1106 36-37 0.34902636954819144 1106 38-39 0.3682199649795024 1106 40-41 0.3978961781243342 1106 42-43 0.3245196401163426 1106 44-45 0.3925996040053974 1106 46-47 0.42609475777914696 1106 48-49 0.44457387996998676 1106 50-51 0.4345610006628391 1106 52-53 0.4936723709662232 1106 54-55 0.4289382023480144 1106 56-57 0.47404643923292156 1106 58-59 0.5632627430909167 1106 60-61 0.58826556239881 1106 62-63 0.5409216222285096 1106 64-65 0.5761043175716196 1106 66-67 0.6067890014606974 1106 68-69 0.599770452595628 1106 70-71 0.59072299214818 1106 72-73 0.5082401145638258 1106 74-75 0.5042609701083407 1106 76-77 0.4708251004759525 1106 78-79 0.6123230532887973 1106 80-81 0.618352439652547 1106 82-83 0.6258254201697291 1106 84-85 0.6070825759266931 1106 86-87 0.5403353062254652 1106 88-89 0.5799681418151934 1106 90-91 0.6268915241982675 1106 92-93 0.6742860758910219 1106 94-95 0.819215141668451 1106 96-97 0.7436711300834666 1106 98-99 0.674450899747761 1106 100 0.7541531664756249 1107 1 0.20355229956538778 1107 2 0.1845077240901034 1107 3 0.22927073698428302 1107 4 0.16502904966426257 1107 5 0.13982780079077628 1107 6 0.16807581864141952 1107 7 0.15253014247053187 1107 8 0.15361246541624496 1107 9 0.21400924791788611 1107 10-11 0.16806797465694956 1107 12-13 0.2130596213369742 1107 14-15 0.24618002872156097 1107 16-17 0.23111530278413284 1107 18-19 0.17809171563978765 1107 20-21 0.23861671741769896 1107 22-23 0.31048046277388863 1107 24-25 0.2905843288333969 1107 26-27 0.3095175218126798 1107 28-29 0.2749802909702481 1107 30-31 0.2410700300430264 1107 32-33 0.24694978855058025 1107 34-35 0.30608128182493033 1107 36-37 0.35484299736592817 1107 38-39 0.3325193605369776 1107 40-41 0.3066341041395688 1107 42-43 0.3760086609643807 1107 44-45 0.33421260328394453 1107 46-47 0.3401076040384652 1107 48-49 0.40038452953222503 1107 50-51 0.36137448270859096 1107 52-53 0.4162134728387912 1107 54-55 0.3696430456431088 1107 56-57 0.3819425296535357 1107 58-59 0.3998363235651681 1107 60-61 0.3896214902847319 1107 62-63 0.390968095487338 1107 64-65 0.5028593830362524 1107 66-67 0.49544734640073074 1107 68-69 0.4233874595851219 1107 70-71 0.4625004901075087 1107 72-73 0.42507226504539375 1107 74-75 0.47440532095646404 1107 76-77 0.43488606293099963 1107 78-79 0.5446385840693395 1107 80-81 0.5580781999460882 1107 82-83 0.5661708050732948 1107 84-85 0.4487581820290991 1107 86-87 0.3751287683313933 1107 88-89 0.42880041498948174 1107 90-91 0.3136407708024187 1107 92-93 0.4000068503986114 1107 94-95 0.5410261031626078 1107 96-97 0.4674605493587798 1107 98-99 0.6081055183109427 1107 100 0.7542426236364612 1108 1 0.21927114916283585 1108 2 0.16864411176833016 1108 3 0.22995366481878676 1108 4 0.14709714265907792 1108 5 0.13704234236355006 1108 6 0.13899827799714615 1108 7 0.16806151666705205 1108 8 0.17083875461928244 1108 9 0.12625475672926711 1108 10-11 0.18196161548842582 1108 12-13 0.22732737260114533 1108 14-15 0.23569656945220885 1108 16-17 0.29150921889132775 1108 18-19 0.28339433915542145 1108 20-21 0.29568633403836486 1108 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-0.2739316034074477 2316 62-63 -0.26572925338892617 2316 64-65 -0.28367077340561764 2316 66-67 -0.07428390705539556 2316 68-69 -0.08741286057844633 2316 70-71 -0.31837555511017257 2316 72-73 -0.4695005399742129 2316 74-75 -0.4559756153650696 2316 76-77 -0.3457098074709002 2316 78-79 -0.30177724547692364 2316 80-81 -0.1807056997109484 2316 82-83 -0.1532389357470727 2316 84-85 -0.1752713885278645 2316 86-87 -0.16670595931226018 2316 88-89 -0.1661867072306471 2316 90-91 -0.059606531977632216 2316 92-93 -0.004996696805129375 2316 94-95 0.041246773269133286 2316 96-97 0.018896008971271527 2316 98-99 -0.09603953862914594 2316 100 -0.3180217772361509 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 17.0 11 50.0 12 107.0 13 258.0 14 753.0 15 2174.0 16 4827.0 17 9979.0 18 17923.0 19 27886.0 20 39757.0 21 54686.0 22 71210.0 23 83150.0 24 89535.0 25 98164.0 26 115740.0 27 139229.0 28 168589.0 29 203669.0 30 245524.0 31 298103.0 32 363030.0 33 456489.0 34 594787.0 35 813140.0 36 1201346.0 37 1900817.0 38 2011654.0 39 337982.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.71371674980108 20.493726417919387 13.581952275430146 24.21060455684939 2 14.891891724130993 21.695049410010085 39.83740255036945 23.57565631548948 3 17.119637172998615 26.968403644183482 31.08220545224399 24.829753730573916 4 11.708783528704666 17.79149292503024 39.046058154743505 31.453665391521586 5 12.21006207907968 38.56195603803992 36.15791167374811 13.07007020913229 6 30.284913480888324 38.85582443808644 17.952380132468605 12.90688194855663 7 26.21430699624689 33.02233151477728 23.4010883958556 17.362273093120237 8 25.09048908756948 36.77032695850255 20.750798747670597 17.38838520625737 9 26.02345144540389 14.975051437661104 20.779435501680688 38.22206161525432 10-11 24.02050575778647 26.759943327955448 29.6493438632342 19.57020705102388 12-13 24.612090806171384 24.581772032337458 29.467923165061823 21.338213996429335 14-15 21.938595232284584 26.05188499356515 26.866010911336357 25.143508862813913 16-17 21.586311171913085 28.886344303568954 27.61745428676357 21.909890237754386 18-19 21.408284215411484 28.12811429156269 29.39715309535817 21.066448397667664 20-21 22.15381763984423 27.37165375518217 29.380475474025946 21.09405313094765 22-23 21.940787595119136 27.332358569604096 29.1034080868458 21.623445748430964 24-25 21.72557474936745 27.446642428043166 29.2748914218822 21.552891400707182 26-27 21.78751804224413 27.585804563106986 29.15633068144272 21.470346713206162 28-29 21.704799274096136 27.497025709558564 29.3348695710343 21.463305445310997 30-31 21.91282341984207 27.466949161922837 29.294470022746953 21.325757395488136 32-33 21.67750315362358 27.5910326399372 29.228954408682878 21.502509797756343 34-35 21.921125986010733 27.608862039430964 29.24803912797751 21.22197284658079 36-37 21.967709396331028 27.521522327844373 29.09721510734909 21.413553168475513 38-39 21.814499369097675 27.69782866610345 29.14952697966359 21.338144985135283 40-41 21.907363854834102 27.54260101196454 29.199604711378345 21.350430421823013 42-43 21.747662395423234 27.545185916656024 29.1099918133773 21.597159874543443 44-45 21.821192855106602 27.773420448545917 28.92813497628085 21.477251720066633 46-47 21.84121163551961 27.680837812000213 28.73264293208959 21.745307620390584 48-49 21.76795061885261 27.769205404648634 28.776285446869842 21.686558529628915 50-51 21.868265947711777 27.933356920119085 28.529573893449616 21.66880323871952 52-53 21.881103592324617 27.999597873112393 28.416505062814945 21.70279347174805 54-55 21.787544744712438 27.98243049647093 28.474901064461744 21.755123694354893 56-57 21.880050261042783 28.016761452445316 28.344437869550372 21.75875041696153 58-59 21.83032859868583 27.92259179398709 28.493283095515437 21.753796511811647 60-61 21.904223015419866 28.026356593375652 28.43752060512613 21.63189978607835 62-63 21.722488688223226 28.02665368320094 28.555996597402146 21.694861031173687 64-65 21.693946133667357 27.85628008473779 28.510246136576367 21.93952764501849 66-67 21.659010252439735 27.918793344411792 28.404403702930058 22.017792700218415 68-69 21.665414900164222 27.94078135471868 28.425964767568246 21.967838977548848 70-71 21.666798483459825 27.782188604751063 28.391706247800492 22.15930666398862 72-73 21.63173531812228 27.942704481358167 28.263953528541606 22.161606671977946 74-75 21.62252403024635 27.98964830359586 28.21305166976878 22.174775996389013 76-77 21.51820709627868 27.97037095343421 28.301385247388826 22.210036702898282 78-79 21.57135255075709 27.85236591555172 28.25823119721969 22.318050336471494 80-81 21.501343837332048 28.08578313149927 28.07445703731194 22.338415993856746 82-83 21.552081309403917 28.010434397357987 28.14343146225588 22.29405283098222 84-85 21.519384528135365 27.978071854717562 28.075494148324797 22.427049468822272 86-87 21.385381343886802 27.94998234200858 28.24188073359902 22.422755580505598 88-89 21.42204029170496 27.840904714961262 28.19731545519426 22.539739538139518 90-91 21.532208245238042 27.938572821490098 28.081695023857268 22.447523909414592 92-93 21.415860867857234 27.826696007441214 28.114273255513844 22.64316986918771 94-95 21.220719931340966 27.74135990169313 28.210170515685256 22.827749651280648 96-97 21.30092765624796 27.699532952110207 28.227360607138724 22.772178784503105 98-99 21.194952663571907 27.643599038221588 28.132745872228597 23.0287024259779 100 20.439330429807345 28.04940023187846 28.341082760545323 23.17018657776887 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2261.0 1 1693.5 2 1788.5 3 2510.5 4 2065.0 5 1931.0 6 3081.5 7 5873.0 8 9138.5 9 10828.5 10 9666.5 11 7902.0 12 8111.5 13 8733.5 14 9201.0 15 9968.5 16 10753.5 17 11557.0 18 12724.0 19 14028.5 20 16009.0 21 18966.5 22 22918.0 23 28649.5 24 36309.0 25 46164.0 26 58323.5 27 72372.0 28 87920.0 29 105619.0 30 125908.0 31 145685.5 32 166926.5 33 191266.0 34 215153.0 35 237120.0 36 263839.5 37 288958.0 38 305407.5 39 318699.5 40 325748.5 41 328447.0 42 331837.0 43 337732.0 44 344963.5 45 353054.0 46 355392.0 47 351511.5 48 351139.5 49 350730.0 50 344729.5 51 328478.0 52 307668.5 53 290292.5 54 271013.5 55 248158.5 56 222203.0 57 195076.0 58 169118.5 59 142381.5 60 116746.5 61 95105.5 62 76155.0 63 58719.5 64 43040.0 65 31134.0 66 22499.5 67 16398.0 68 12153.5 69 8952.5 70 6825.0 71 5061.5 72 3775.5 73 2879.0 74 2162.0 75 1504.5 76 957.0 77 600.5 78 395.5 79 268.5 80 188.0 81 126.0 82 92.5 83 73.5 84 54.5 85 44.5 86 35.0 87 22.5 88 13.5 89 10.5 90 13.0 91 10.5 92 8.0 93 6.0 94 3.0 95 4.0 96 5.5 97 6.0 98 4.0 99 4.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034436138181741962 2 0.07041227757409597 3 7.48611699603086E-4 4 7.700006053060315E-4 5 0.001593473474869426 6 5.240281897221602E-4 7 0.0 8 7.165283410486681E-4 9 3.208335855441797E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.272227939001407E-4 18-19 0.001074792511573002 20-21 0.003315280383956524 22-23 0.0 24-25 1.0694452851472658E-5 26-27 1.871529249007715E-4 28-29 9.892368887612208E-4 30-31 0.0017164596826613615 32-33 0.0030693079683726527 34-35 0.004293822819866273 36-37 0.001283334342176719 38-39 0.0015560428898892717 40-41 0.005475559859954001 42-43 0.006764241428556457 44-45 0.005299101387904702 46-47 0.007149241731209472 48-49 0.008972645942385562 50-51 0.008277506507039838 52-53 0.0038446558001044204 54-55 0.002518543646521811 56-57 0.0027698632885314184 58-59 0.003374099874639624 60-61 0.007737436638040469 62-63 0.014063205499686544 64-65 0.004128058800668446 66-67 0.010320147001671116 68-69 0.01270500998754952 70-71 0.00581243512477539 72-73 0.007817645034426512 74-75 0.0031067385533528075 76-77 0.0031067385533528075 78-79 0.007929936789366977 80-81 0.0059033379740129075 82-83 0.0167207770332775 84-85 0.017672583337058567 86-87 0.02335133780119055 88-89 0.021581405854271825 90-91 0.024992936313891604 92-93 0.03465537446519715 94-95 0.038328919019678005 96-97 0.0228433512907456 98-99 0.018298208828869716 100 0.01902543162276986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9350642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.943885174159504 #Duplication Level Percentage of deduplicated Percentage of total 1 71.66231076015855 42.2405501676172 2 16.976294558669384 20.01297514297834 3 5.717906403022427 10.11106855369036 4 2.3249849686182267 5.4817458808751836 5 1.1363664575605135 3.3490926995106656 6 0.6404677097660647 2.26509930853247 7 0.388749012263192 1.6040063998286658 8 0.24885999174617965 1.1735019822343276 9 0.17389684052454962 0.9225139860025362 >10 0.6765906787800573 6.63444348980858 >50 0.030098124380105076 1.2381939032312685 >100 0.021087694060690693 2.4247570390606366 >500 0.0014688312079179453 0.5906917136818463 >1k 8.392861648949858E-4 0.8236113080315377 >5k 0.0 0.0 >10k+ 7.868307720560052E-5 1.127748424916289 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28598 0.30583996264641505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26249 0.2807186928983058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18101 0.19358029106450658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.277781140589063E-5 2 0.0 0.0 0.0 0.0 4.277781140589063E-5 3 0.0 0.0 0.0 0.0 4.277781140589063E-5 4 0.0 0.0 0.0 0.0 4.277781140589063E-5 5 0.0 0.0 0.0 0.0 5.347226425736329E-5 6 0.0 0.0 0.0 0.0 6.416671710883594E-5 7 0.0 0.0 0.0 0.0 6.416671710883594E-5 8 3.208335855441797E-5 0.0 0.0 0.0 6.416671710883594E-5 9 3.208335855441797E-5 0.0 0.0 0.0 6.416671710883594E-5 10-11 4.277781140589063E-5 0.0 0.0 1.6041679277208986E-5 7.48611699603086E-5 12-13 4.277781140589063E-5 0.0 0.0 5.8819490683099615E-5 1.1763898136619924E-4 14-15 4.277781140589063E-5 0.0 0.0 9.625007566325392E-5 1.5506956634635352E-4 16-17 5.347226425736329E-5 0.0 0.0 1.0694452851472658E-4 1.818056984750352E-4 18-19 5.347226425736329E-5 0.0 0.0 1.1763898136619924E-4 1.9250015132650785E-4 20-21 5.347226425736329E-5 0.0 0.0 1.336806606434082E-4 2.3527796273239849E-4 22-23 5.347226425736329E-5 0.0 0.0 1.497223399206172E-4 2.566668684353438E-4 24-25 5.347226425736329E-5 0.0 0.0 2.1388905702945317E-4 2.780557741382891E-4 26-27 5.347226425736329E-5 0.0 0.0 4.0104198193022467E-4 3.0479190626697076E-4 28-29 6.416671710883594E-5 0.0 0.0 0.0013421538328598187 3.1548635911844345E-4 30-31 6.416671710883594E-5 0.0 0.0 0.0053151430671819115 3.2083358554417974E-4 32-33 6.416671710883594E-5 0.0 0.0 0.013432232781449657 3.2083358554417974E-4 34-35 6.416671710883594E-5 1.6041679277208986E-5 0.0 0.026720090449404436 3.315280383956524E-4 36-37 6.416671710883594E-5 3.74305849801543E-5 0.0 0.048889691210507256 3.315280383956524E-4 38-39 7.48611699603086E-5 4.277781140589063E-5 0.0 0.08297291244815061 3.4222249124712505E-4 40-41 7.48611699603086E-5 5.347226425736329E-5 0.0 0.12066551152316601 3.4222249124712505E-4 42-43 7.48611699603086E-5 5.347226425736329E-5 0.0 0.15866290250444837 3.475697176728614E-4 44-45 7.48611699603086E-5 5.347226425736329E-5 0.0 0.20730662129937175 3.5291694409859774E-4 46-47 8.020839638604493E-5 5.347226425736329E-5 0.0 0.26671965411572807 3.5291694409859774E-4 48-49 8.555562281178126E-5 5.347226425736329E-5 0.0 0.33131949656504867 3.5291694409859774E-4 50-51 8.555562281178126E-5 5.347226425736329E-5 0.0 0.4019135798376197 3.636113969500704E-4 52-53 8.555562281178126E-5 5.347226425736329E-5 0.0 0.47003724450150053 3.956947555044883E-4 54-55 8.555562281178126E-5 5.347226425736329E-5 0.0 0.5514273779276332 4.2777811405890633E-4 56-57 8.555562281178126E-5 5.347226425736329E-5 0.0 0.6483137735355498 4.4916701976185165E-4 58-59 8.555562281178126E-5 5.347226425736329E-5 0.0 0.7578356651874812 4.598614726133243E-4 60-61 8.555562281178126E-5 6.951394353457227E-5 0.0 0.8766938141787484 4.598614726133243E-4 62-63 8.555562281178126E-5 7.48611699603086E-5 0.0 0.9984608543456162 4.7055592546479697E-4 64-65 8.555562281178126E-5 7.48611699603086E-5 0.0 1.1270830387902777 4.7055592546479697E-4 66-67 8.555562281178126E-5 9.625007566325392E-5 0.0 1.276158364313381 4.7055592546479697E-4 68-69 8.555562281178126E-5 9.625007566325392E-5 0.0 1.4510394045670876 4.812503783162696E-4 70-71 8.555562281178126E-5 9.625007566325392E-5 0.0 1.6435930281578526 5.240281897221602E-4 72-73 8.555562281178126E-5 9.625007566325392E-5 5.347226425736329E-6 1.8389218622635752 5.668060011280509E-4 74-75 8.555562281178126E-5 9.625007566325392E-5 1.0694452851472658E-5 2.034379029803515 5.668060011280509E-4 76-77 8.555562281178126E-5 9.625007566325392E-5 1.0694452851472658E-5 2.252187603803033 5.935421332567325E-4 78-79 8.555562281178126E-5 9.625007566325392E-5 1.0694452851472658E-5 2.502657036810948 6.095838125339415E-4 80-81 8.555562281178126E-5 9.625007566325392E-5 1.0694452851472658E-5 2.778354684095488 6.363199446626232E-4 82-83 8.555562281178126E-5 9.625007566325392E-5 1.0694452851472658E-5 3.0691903293912866 6.790977560685138E-4 84-85 1.0694452851472658E-4 9.625007566325392E-5 1.0694452851472658E-5 3.358207917702335 6.951394353457228E-4 86-87 1.0694452851472658E-4 9.625007566325392E-5 1.0694452851472658E-5 3.6567114856926404 7.058338881971954E-4 88 1.0694452851472658E-4 9.625007566325392E-5 1.0694452851472658E-5 3.9032079294662334 7.165283410486681E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15560 0.0 48.295605 1 GTATCAA 25070 0.0 34.214554 1 TCAACGC 30395 0.0 27.581305 4 ATCAACG 30570 0.0 27.438786 3 CAACGCA 30840 0.0 27.152416 5 AACGCAG 31850 0.0 26.320606 6 TATCAAC 32655 0.0 25.96405 2 TACCTGG 7555 0.0 24.461582 2 GTACATG 29125 0.0 24.171091 1 TACATGG 29370 0.0 23.42431 2 ACATGGG 28680 0.0 23.004356 3 ACGCAGA 36520 0.0 22.826185 7 GTACCTG 8470 0.0 22.652552 1 CGCAGAG 37380 0.0 22.225597 8 CATGGGG 15250 0.0 21.200235 4 TATAACG 815 0.0 20.194674 2 GCAGAGT 41925 0.0 19.446297 9 GAGTACT 25985 0.0 19.115412 12-13 TAACGCA 880 0.0 18.68992 4 ACCTGGG 9775 0.0 18.556355 3 >>END_MODULE