FastQCFastQC Report
Mon 6 Jun 2016
ERR1211488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7004383
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217390.31036281139966215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190240.27160136731529383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121960.1741195477174792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114100.046.7504041
TACCTGG68550.036.491572
GTATCAA191250.036.261221
GTACCTG76850.033.081941
TCAACGC225350.029.882894
ATCAACG226750.029.6368483
CAACGCA228800.029.4503125
ACCTGGG83450.029.3396343
AACGCAG237800.028.410086
TATCAAC239450.028.2575382
TATAACG5850.026.5244522
GTACATG217000.025.9485171
TACATGG223550.024.567252
ACGCAGA273200.024.5412467
ACATGGG220950.024.055363
CGCAGAG281250.023.7051778
CATGGGG140050.022.8841744
GCAGAGT309100.020.7349419
TAACGCA7900.020.2248734
GAGTACT184000.019.868212-13
Produced by FastQC (version 0.11.5)