FastQCFastQC Report
Mon 6 Jun 2016
ERR1211488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7004383
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245560.350580486532504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217930.31113375724885406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149940.21406596412560536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122800.046.17521
TACCTGG67800.037.5846752
GTATCAA207000.036.6373371
GTACCTG77500.034.3986361
ACCTGGG85350.029.1809623
TCAACGC256650.029.0026784
ATCAACG259600.028.6552053
CAACGCA261500.028.5546195
TATCAAC267600.027.95282
AACGCAG271350.027.5871626
GTACATG235650.025.4591031
ACGCAGA306900.024.1770467
TACATGG241800.024.0716362
CGCAGAG315850.023.4175688
ACATGGG240450.023.2567733
CATGGGG143000.022.0172424
TATAACG6700.021.0517252
GCAGAGT352950.020.569959
TAACGCA7550.019.917154
GAGTACT210100.019.63793212-13
Produced by FastQC (version 0.11.5)