FastQCFastQC Report
Mon 6 Jun 2016
ERR1211487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6169290
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200920.32567767117447877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178140.2887528386572847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106550.17271031188353927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108000.046.9549981
GTATCAA182650.036.9932171
TCAACGC220200.029.9810264
ATCAACG221450.029.8344653
CAACGCA221400.029.8170785
TACCTGG51350.029.3007552
AACGCAG230500.028.7156336
TATCAAC232300.028.700952
GTACCTG55750.028.0403271
GTACATG217350.026.2345941
ACGCAGA258050.025.4326747
TACATGG219400.025.309442
CGCAGAG261800.024.962338
ACATGGG216750.024.6496053
CATGGGG129450.022.4323044
GCAGAGT287100.022.0939839
GAGTACT166350.021.19977412-13
ACCTGGG69050.020.8921593
TATACCG4350.020.522525
ATGGGGG81500.020.1779375
Produced by FastQC (version 0.11.5)