FastQCFastQC Report
Mon 6 Jun 2016
ERR1211487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6169290
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218300.35384947052253984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201570.3267312770189114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132630.21498422022631453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117600.047.2230831
GTATCAA193950.035.3976861
TACCTGG51650.028.3131242
GTACCTG54550.027.6708221
TCAACGC244300.027.5648964
ATCAACG244450.027.4518623
CAACGCA246900.027.255375
AACGCAG254900.026.4177536
TATCAAC255800.026.3600542
GTACATG215100.025.6648961
TACATGG221050.024.4627362
ACATGGG218850.023.5770473
ACGCAGA288650.023.1657227
CATGGGG125900.022.8059984
CGCAGAG293250.022.7702888
ATGGGGG74250.022.2147185
ACCTGGG66950.021.4083353
GAGTACT187300.020.81141912-13
GCAGAGT321950.020.4337759
AGAGTAC293550.019.6735310-11
Produced by FastQC (version 0.11.5)