FastQCFastQC Report
Mon 6 Jun 2016
ERR1211486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141170.38508225653783307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124310.33909170015030127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77510.21143108099629837No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.14776443887975077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.048.075761
TACCTGG43650.047.0701982
GTACCTG49800.042.0728951
ACCTGGG51800.039.096293
GTATCAA116800.036.1997221
TCAACGC143450.028.7919084
ATCAACG145050.028.4423073
CAACGCA145650.028.2917215
TATCAAC146750.028.1937852
AACGCAG152450.027.4578956
TATAACG3800.027.2199862
CCTGGGG47700.026.202664
GTACATG131900.026.1656131
TACATGG133400.025.1646672
ACGCAGA169050.024.5671087
CGCAGAG172700.024.0488688
TATCACG4150.023.79142
ACATGGG135450.023.7629093
CATGGGG80000.022.4952534
GCAGAGT186600.021.553349
Produced by FastQC (version 0.11.5)