FastQCFastQC Report
Mon 6 Jun 2016
ERR1211486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665970
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154940.42264393871199163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138730.37842644647937657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94820.2586491433372341No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52520.14326358371726994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50000.049.0872572
GTACCTG54050.046.453641
ACCTGGG55400.043.176873
TATAACG4600.042.9298862
GGTATCA80650.042.5008241
TAACGCA4750.038.5840844
GTATCAA129050.035.30531
ATAACGC5300.034.5805443
CCTGGGG51350.031.115424
TCAACGC160200.028.0141874
ATCAACG160450.027.8244743
CAACGCA163900.027.352735
TATCAAC166300.026.8313312
AACGCAG169800.026.401596
GTACATG133500.023.6328141
ACGCAGA191150.023.4032487
CGCAGAG193500.023.0461678
TACATGG135200.022.7789062
CATGGGG77050.022.6885874
ACATGGG130050.022.2955363
Produced by FastQC (version 0.11.5)