##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3665970 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1023450819292 33.0 31.0 34.0 30.0 34.0 2 32.320376598826506 34.0 31.0 34.0 30.0 34.0 3 32.333734045832344 34.0 31.0 34.0 30.0 34.0 4 35.97818530975431 37.0 35.0 37.0 35.0 37.0 5 35.902687419700655 37.0 35.0 37.0 35.0 37.0 6 35.921912072384664 37.0 35.0 37.0 35.0 37.0 7 35.85366383249181 37.0 35.0 37.0 35.0 37.0 8 35.846536660147244 37.0 35.0 37.0 35.0 37.0 9 37.49665736489933 39.0 37.0 39.0 35.0 39.0 10-11 37.455610520544354 39.0 37.0 39.0 34.5 39.0 12-13 37.36521479990289 39.0 37.0 39.0 34.0 39.0 14-15 38.5910030633093 40.0 38.0 41.0 34.0 41.0 16-17 38.51659942661833 40.0 38.0 41.0 34.0 41.0 18-19 38.50899898253395 40.0 38.0 41.0 34.0 41.0 20-21 38.44410115194614 40.0 38.0 41.0 34.0 41.0 22-23 38.32332820508624 40.0 38.0 41.0 34.0 41.0 24-25 38.20730747933017 40.0 38.0 41.0 33.5 41.0 26-27 38.0228646170045 40.0 37.0 41.0 33.0 41.0 28-29 38.052281251619625 40.0 37.0 41.0 33.0 41.0 30-31 37.958150912309705 40.0 37.0 41.0 33.0 41.0 32-33 37.752706787016805 40.0 37.0 41.0 33.0 41.0 34-35 37.61673036058669 40.0 37.0 41.0 33.0 41.0 36-37 37.54067518283019 40.0 37.0 41.0 32.0 41.0 38-39 37.429570345638396 40.0 36.5 41.0 32.0 41.0 40-41 37.26289290419725 40.0 36.0 41.0 31.0 41.0 42-43 37.10590539475228 39.5 36.0 41.0 31.0 41.0 44-45 36.91335471921484 39.0 36.0 41.0 30.5 41.0 46-47 36.64001873992422 39.0 35.0 41.0 30.0 41.0 48-49 36.40108934333887 39.0 35.0 41.0 29.5 41.0 50-51 36.19179685049251 39.0 35.0 40.0 28.5 41.0 52-53 35.98694670169151 39.0 35.0 40.0 28.0 41.0 54-55 35.78387329956328 38.0 35.0 40.0 28.0 41.0 56-57 35.576419610635114 38.0 34.0 40.0 27.0 41.0 58-59 35.30847756528286 38.0 34.0 40.0 26.5 41.0 60-61 34.98158822903625 38.0 34.0 40.0 26.0 41.0 62-63 34.64701729692278 37.0 33.0 40.0 26.0 41.0 64-65 34.331314358819085 36.5 33.0 39.5 25.5 41.0 66-67 33.98694220083634 36.0 32.5 39.0 25.0 40.5 68-69 33.58521591829721 35.5 32.0 39.0 23.5 40.0 70-71 33.19059280354176 35.0 32.0 38.0 23.5 40.0 72-73 32.78050488683759 35.0 31.0 37.5 22.5 40.0 74-75 32.36956317154805 35.0 31.0 37.0 22.0 39.0 76-77 30.842782128604433 33.5 29.5 35.5 20.5 37.5 78-79 31.67215511856344 34.0 30.5 36.0 21.0 38.0 80-81 31.648450205539053 34.0 31.0 36.0 21.0 37.5 82-83 31.365231984986238 34.0 30.5 35.0 20.0 37.0 84-85 31.07374351126714 34.0 30.0 35.0 20.0 36.5 86-87 30.777751454594554 34.0 30.0 35.0 20.0 36.0 88-89 30.508073443045088 34.0 29.5 35.0 19.0 36.0 90-91 30.217502871000036 34.0 29.0 35.0 18.0 35.0 92-93 30.007340349211805 34.0 29.0 35.0 18.0 35.0 94-95 29.785855585288477 34.0 29.0 35.0 18.0 35.0 96-97 29.58172366385977 33.5 29.0 35.0 17.0 35.0 98-99 29.411875847320083 33.0 28.5 35.0 16.5 35.0 100 27.52062482780819 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3426526932335534 1101 2 0.28412890181041917 1101 3 0.25939935116240775 1101 4 0.16434977411287122 1101 5 0.17491809084777543 1101 6 0.1762923013080595 1101 7 0.24486858352623386 1101 8 0.1844795534707231 1101 9 0.18405473788561721 1101 10-11 0.21387755788569507 1101 12-13 0.25908816287092407 1101 14-15 0.29298923786195985 1101 16-17 0.30701420674188995 1101 18-19 0.34073245215772374 1101 20-21 0.28843232293382925 1101 22-23 0.31501760566967363 1101 24-25 0.35602833965796066 1101 26-27 0.177881030574504 1101 28-29 0.29048119446493814 1101 30-31 0.29484859552844256 1101 32-33 0.33097971418953165 1101 34-35 0.35436762516929576 1101 36-37 0.4197078270736654 1101 38-39 0.35707567350631564 1101 40-41 0.39576783319915165 1101 42-43 0.293540205209986 1101 44-45 0.3365255042414148 1101 46-47 0.47198160147613777 1101 48-49 0.4904326937127408 1101 50-51 0.3571507497647488 1101 52-53 0.31843438956899206 1101 54-55 0.3406236419651876 1101 56-57 0.2859051842755207 1101 58-59 0.3827776916207597 1101 60-61 0.2899263487137915 1101 62-63 0.3435223596173955 1101 64-65 0.33161587567844464 1101 66-67 0.19357577584343488 1101 68-69 0.25561401630228175 1101 70-71 0.32199531658008596 1101 72-73 0.18680398560388056 1101 74-75 0.18007760970118625 1101 76-77 0.24959212143000897 1101 78-79 0.2857802709073418 1101 80-81 0.2501408774314875 1101 82-83 0.2939560906027978 1101 84-85 0.20643315441854426 1101 86-87 0.15261584949955775 1101 88-89 0.11086403372655695 1101 90-91 0.03344960222245419 1101 92-93 -0.006868221622816861 1101 94-95 0.060979751764214996 1101 96-97 0.07151167175851825 1101 98-99 -0.10145238117702249 1101 100 -0.2111576812303717 1102 1 0.25435774531521815 1102 2 0.1637674023476876 1102 3 0.27120253867096267 1102 4 0.1629680156541511 1102 5 0.10949423025249416 1102 6 0.12291454009649527 1102 7 0.17240949998105037 1102 8 0.17211094671229432 1102 9 0.15652270312374128 1102 10-11 0.17427964619981395 1102 12-13 0.20131141562837485 1102 14-15 0.24516386941729706 1102 16-17 0.2572809730000003 1102 18-19 0.29750481582373567 1102 20-21 0.315635419587025 1102 22-23 0.19116882838273597 1102 24-25 0.2077932485259666 1102 26-27 0.19422528693414165 1102 28-29 0.2533734328359927 1102 30-31 0.32131240044569864 1102 32-33 0.3627005834500423 1102 34-35 0.2215897131145539 1102 36-37 0.26258582561570876 1102 38-39 0.2525473690554776 1102 40-41 0.2635940461326314 1102 42-43 0.361916622034137 1102 44-45 0.3012638485967827 1102 46-47 0.3593343134015825 1102 48-49 0.3818977993369401 1102 50-51 0.36836497911044574 1102 52-53 0.3895796620331211 1102 54-55 0.4183465845913972 1102 56-57 0.3642855177806652 1102 58-59 0.4113118288686337 1102 60-61 0.3039002122696317 1102 62-63 0.28161404780882293 1102 64-65 0.35411664979321245 1102 66-67 0.36189526247187587 1102 68-69 0.3659281612961607 1102 70-71 0.25764878592465124 1102 72-73 0.27471891354569067 1102 74-75 0.17925411175077244 1102 76-77 0.24883936155312725 1102 78-79 0.39678480979216957 1102 80-81 0.3691601735160681 1102 82-83 0.17683337569737745 1102 84-85 0.13803077198934588 1102 86-87 0.13052530767745907 1102 88-89 0.07524249964949448 1102 90-91 0.17940918421018992 1102 92-93 0.07452472873968219 1102 94-95 0.17919908881135527 1102 96-97 0.18785845187789008 1102 98-99 0.18184741527204906 1102 100 0.146618022534593 1103 1 0.22138370208590885 1103 2 0.19897588701682878 1103 3 0.3051916625676867 1103 4 0.10138806610892459 1103 5 0.1402009316065076 1103 6 0.19040944269757887 1103 7 0.18070009992389657 1103 8 0.19089567030278687 1103 9 0.2350533783497255 1103 10-11 0.20742643220521728 1103 12-13 0.2243171225837628 1103 14-15 0.33210856768870656 1103 16-17 0.3277029300690799 1103 18-19 0.31259830882392947 1103 20-21 0.23392133292171025 1103 22-23 0.36108783630883323 1103 24-25 0.3095265996044674 1103 26-27 0.35871113801201915 1103 28-29 0.2894912954551714 1103 30-31 0.39590149817077247 1103 32-33 0.5096021740962513 1103 34-35 0.4307811220239728 1103 36-37 0.5183742266198479 1103 38-39 0.4507038417917357 1103 40-41 0.4916717610505472 1103 42-43 0.5380255278040735 1103 44-45 0.5279535492858471 1103 46-47 0.6506775830381812 1103 48-49 0.6626773959119205 1103 50-51 0.65672149640789 1103 52-53 0.6508814263143208 1103 54-55 0.5820730304199273 1103 56-57 0.5974730544761115 1103 58-59 0.6554288468574327 1103 60-61 0.6220750572038725 1103 62-63 0.7995612353606347 1103 64-65 0.7225612535145842 1103 66-67 0.6783159751485996 1103 68-69 0.7050462090499963 1103 70-71 0.6832458142036586 1103 72-73 0.6734213538286085 1103 74-75 0.6085685174821691 1103 76-77 0.6227696327047632 1103 78-79 0.624481412509315 1103 80-81 0.6072466532091205 1103 82-83 0.6726576846457277 1103 84-85 0.6087787841462742 1103 86-87 0.5146734255618881 1103 88-89 0.5413713774088222 1103 90-91 0.5706868760296402 1103 92-93 0.5325721814528919 1103 94-95 0.5351968567863352 1103 96-97 0.6372771612836523 1103 98-99 0.6732083557263486 1103 100 0.7781058211199827 1104 1 0.2644806702643052 1104 2 0.20924900152447634 1104 3 0.27788021180346334 1104 4 0.07457774288166519 1104 5 0.15297968379893945 1104 6 0.16710701222902458 1104 7 0.18746532167283192 1104 8 0.1721033876668514 1104 9 0.20398347105831505 1104 10-11 0.2090550967003324 1104 12-13 0.24874709001047535 1104 14-15 0.32697201043488633 1104 16-17 0.29162174740809377 1104 18-19 0.3123477450554546 1104 20-21 0.22828364813092605 1104 22-23 0.2896771622922998 1104 24-25 0.30401419669793484 1104 26-27 0.3762506018055589 1104 28-29 0.42178896521212295 1104 30-31 0.3609478524679446 1104 32-33 0.32769287818045 1104 34-35 0.4122394031565193 1104 36-37 0.36978991191175226 1104 38-39 0.3627559590555762 1104 40-41 0.4024710594719991 1104 42-43 0.4916712306354398 1104 44-45 0.5709252355800061 1104 46-47 0.4655109581321497 1104 48-49 0.48703219420809063 1104 50-51 0.3829805793445047 1104 52-53 0.4337679209272025 1104 54-55 0.47533286504784655 1104 56-57 0.5586356703618591 1104 58-59 0.5568320039609205 1104 60-61 0.5347535838889073 1104 62-63 0.6296790003318193 1104 64-65 0.621584054817518 1104 66-67 0.6159255967973039 1104 68-69 0.5760058682832678 1104 70-71 0.5024640552460085 1104 72-73 0.5738222558581683 1104 74-75 0.5584557637863554 1104 76-77 0.4567711360873723 1104 78-79 0.6679542263404308 1104 80-81 0.7802609353439358 1104 82-83 0.5786962714660717 1104 84-85 0.5814673333820544 1104 86-87 0.5720525285435976 1104 88-89 0.6071443666345786 1104 90-91 0.5506417745513161 1104 92-93 0.5496105177094712 1104 94-95 0.6201379743007429 1104 96-97 0.46476400668580453 1104 98-99 0.43868066842992803 1104 100 0.4987272192658132 1105 1 0.29593672610120336 1105 2 0.21054232139363904 1105 3 0.21230459241390065 1105 4 0.1674852250361809 1105 5 0.17619019566926397 1105 6 0.15008262847927512 1105 7 0.2158192701579793 1105 8 0.2004737432665138 1105 9 0.23544611557899486 1105 10-11 0.2696998614194044 1105 12-13 0.2872414749985026 1105 14-15 0.35523594587250784 1105 16-17 0.31845903902240025 1105 18-19 0.33837944088770655 1105 20-21 0.31117768743268215 1105 22-23 0.40013250558242675 1105 24-25 0.3788543452171993 1105 26-27 0.39121682931061486 1105 28-29 0.4989952586453583 1105 30-31 0.48403932232513114 1105 32-33 0.44302132826557994 1105 34-35 0.5041814170548946 1105 36-37 0.5844082116967328 1105 38-39 0.5741874029288141 1105 40-41 0.5396747148479264 1105 42-43 0.4068531018849413 1105 44-45 0.5733509370277545 1105 46-47 0.5876727453294208 1105 48-49 0.7229605904597989 1105 50-51 0.6364271230598675 1105 52-53 0.5790134847244346 1105 54-55 0.533963430378229 1105 56-57 0.5057073524638085 1105 58-59 0.5479679311557035 1105 60-61 0.6250363152910765 1105 62-63 0.7134117403218809 1105 64-65 0.7930077188762965 1105 66-67 0.7251540561130483 1105 68-69 0.7215382982047132 1105 70-71 0.692513476187905 1105 72-73 0.6260764286750842 1105 74-75 0.714932320442017 1105 76-77 0.7336593769598316 1105 78-79 0.6952065404379688 1105 80-81 0.7883745118500833 1105 82-83 0.8132634262702396 1105 84-85 0.7718093048027974 1105 86-87 0.7591000611816447 1105 88-89 0.6635110085420095 1105 90-91 0.8446833952667561 1105 92-93 0.7841713422096106 1105 94-95 0.7388933796859831 1105 96-97 0.7517752434276126 1105 98-99 0.7674561828656401 1105 100 0.856800026676197 1106 1 0.2929596860716259 1106 2 0.2545370240375604 1106 3 0.26749484117794253 1106 4 0.17192864428687926 1106 5 0.1443905378309367 1106 6 0.16669722352089877 1106 7 0.22575321494242928 1106 8 0.1859397306705901 1106 9 0.21144086491749903 1106 10-11 0.1965807544492435 1106 12-13 0.2041429677706006 1106 14-15 0.2961481348326629 1106 16-17 0.30673429858651957 1106 18-19 0.26850925898038014 1106 20-21 0.28839790850540936 1106 22-23 0.3070223488406043 1106 24-25 0.311841858266078 1106 26-27 0.3210462630273696 1106 28-29 0.3380491339461926 1106 30-31 0.41673714640358384 1106 32-33 0.5026676807703581 1106 34-35 0.4961165595943271 1106 36-37 0.4884185452844747 1106 38-39 0.5181429564757138 1106 40-41 0.5121799903240642 1106 42-43 0.49075790573282774 1106 44-45 0.4381882393673635 1106 46-47 0.5476906131781618 1106 48-49 0.6160014161920628 1106 50-51 0.6529236773458678 1106 52-53 0.6657221478137458 1106 54-55 0.5259640822762393 1106 56-57 0.6474250619748929 1106 58-59 0.7276164396977123 1106 60-61 0.6994157192791732 1106 62-63 0.678489969713155 1106 64-65 0.7914419180353107 1106 66-67 0.7638636306596069 1106 68-69 0.676884882493276 1106 70-71 0.7497935354456686 1106 72-73 0.7061163760145561 1106 74-75 0.7387662815760905 1106 76-77 0.6840372280272256 1106 78-79 0.7348971575739576 1106 80-81 0.7737513165285641 1106 82-83 0.8138559778202961 1106 84-85 0.8232339556385675 1106 86-87 0.7309376890137464 1106 88-89 0.6946605292019754 1106 90-91 0.7531130508349193 1106 92-93 0.7205763633894477 1106 94-95 0.8715446461426417 1106 96-97 0.8537837166669568 1106 98-99 0.9164194752557897 1106 100 1.0020579806177352 1107 1 0.14227607416148658 1107 2 0.07507957063609894 1107 3 0.1554275409762198 1107 4 0.11485462414264447 1107 5 0.07873149955658221 1107 6 0.134702977322668 1107 7 0.06833967306943123 1107 8 0.07281376084092983 1107 9 0.12331438282834739 1107 10-11 0.14157835681705677 1107 12-13 0.18345158240312287 1107 14-15 0.1864409977756054 1107 16-17 0.16084778745515393 1107 18-19 0.13019784154739256 1107 20-21 0.06764779693593681 1107 22-23 0.1774279127390841 1107 24-25 0.15379955813202173 1107 26-27 0.16771185319734627 1107 28-29 0.15440465183151986 1107 30-31 0.09224849414349734 1107 32-33 0.20821544468355313 1107 34-35 0.2501976569095845 1107 36-37 0.29717371088991484 1107 38-39 0.37185797833405587 1107 40-41 0.37243286094252426 1107 42-43 0.3004583028724781 1107 44-45 0.38454893526190403 1107 46-47 0.3757493919592534 1107 48-49 0.3639659740206227 1107 50-51 0.318009062305336 1107 52-53 0.4275843058064268 1107 54-55 0.39484340283070907 1107 56-57 0.35168866542856847 1107 58-59 0.4220336746072846 1107 60-61 0.4252783739380419 1107 62-63 0.5062390086950046 1107 64-65 0.5959829921616446 1107 66-67 0.5327616567401208 1107 68-69 0.49319329827890357 1107 70-71 0.625694967181964 1107 72-73 0.6277361946708311 1107 74-75 0.6163953165217322 1107 76-77 0.511144718369362 1107 78-79 0.5939848150325382 1107 80-81 0.6755667641872982 1107 82-83 0.6687042530006835 1107 84-85 0.5032453903015153 1107 86-87 0.5267154207132272 1107 88-89 0.5972765514676723 1107 90-91 0.48744287172193523 1107 92-93 0.4832534328114946 1107 94-95 0.7072072244845167 1107 96-97 0.7066679760473313 1107 98-99 0.7643911660562139 1107 100 0.9638673275558212 1108 1 0.2113184529800094 1108 2 0.11918964391796294 1108 3 0.17626703875092176 1108 4 0.15571101629974748 1108 5 0.09835997058192447 1108 6 0.11508302212354238 1108 7 0.15313277153882154 1108 8 0.15730524653993427 1108 9 0.1508782057683007 1108 10-11 0.17281040367596034 1108 12-13 0.17305598287370572 1108 14-15 0.1939872908237419 1108 16-17 0.2143382975201007 1108 18-19 0.2630810816896272 1108 20-21 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-0.03008075317794834 2316 92-93 0.1630698267428059 2316 94-95 0.027361995212615398 2316 96-97 -0.012967356962011678 2316 98-99 -0.11915296590589364 2316 100 -0.17815574414002455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 3.0 11 9.0 12 39.0 13 130.0 14 419.0 15 1227.0 16 2568.0 17 5582.0 18 9803.0 19 14869.0 20 19911.0 21 25937.0 22 33016.0 23 37741.0 24 40122.0 25 43434.0 26 49137.0 27 58557.0 28 69901.0 29 83504.0 30 100879.0 31 122037.0 32 150253.0 33 188462.0 34 247305.0 35 337255.0 36 486649.0 37 741745.0 38 696764.0 39 98704.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.06646102075154 19.60045806590999 13.860367369296272 23.472713544042197 2 15.373392380821358 21.408235639657462 39.83213283937839 23.38623914014279 3 17.296528200967938 27.581944921054312 30.84220191394399 24.279324964033762 4 12.070922016769495 17.77592225954475 37.989771796716916 32.16338392696884 5 12.3643678245577 37.65212203642827 35.06770117698599 14.915808962028038 6 29.536611499550457 37.47321296078072 19.04105672960257 13.949118810066253 7 26.368382719989526 32.92449201711962 23.06805565784772 17.63906960504314 8 25.9226697232741 35.40207172079833 20.88415025022245 17.791108305705116 9 26.76227574148179 15.542434880809171 20.733557557754157 36.961731819954885 10-11 24.56727687351506 26.26786362136079 29.21053636554582 19.95432313957834 12-13 25.09049446667594 24.305040685002876 28.957874723470184 21.646590124850995 14-15 22.567328701544202 25.526231802224242 26.941014247252433 24.965425248979123 16-17 22.001318077963003 28.06987777227366 27.61243761497721 22.316366534786127 18-19 21.7576458046685 27.159218992605428 29.5947660785852 21.488369124140874 20-21 22.700100017444743 26.62991404064612 29.425669634728322 21.24431630718081 22-23 22.454728551342285 26.445841952585802 29.295961136610654 21.803468359461256 24-25 22.04363213208626 26.594640215080982 29.436140740999377 21.92558691183338 26-27 21.966855102777878 26.584439999956356 29.42336585956654 22.025339037699222 28-29 21.983923058069657 26.789752187266902 29.448392885468326 21.77793186919512 30-31 22.52150892545391 26.632659741620113 29.458376069308663 21.38745526361732 32-33 22.226488309367852 26.70291097933979 29.41037750324787 21.660223208044492 34-35 22.446248072055873 26.86664818968297 29.231533882441646 21.455569855819515 36-37 22.433190509086717 26.860323888020154 29.107626923882158 21.598858679010974 38-39 22.31140561984977 27.031944126386946 28.989558272808452 21.66709198095483 40-41 22.427298046263928 26.73745485772913 29.143974194790918 21.691272901216017 42-43 22.164266531795903 26.94531773535514 28.914747302141468 21.975668430707486 44-45 22.160752854267812 27.11389723749295 28.854521510755205 21.87082839748403 46-47 22.154983677838047 26.831143609791415 28.642751501989444 22.371121210381094 48-49 22.125209171177453 27.004401392601274 28.70357791152366 22.166811524697614 50-51 22.207863773880355 27.311160033500016 28.416987890344743 22.063988302274893 52-53 22.25908972059674 27.34650442785066 28.179330713735943 22.215075137816655 54-55 22.11950642770855 27.25548104121609 28.417069104567204 22.20794342650816 56-57 22.261551958169843 27.339075123190337 28.08065857182138 22.31871434681844 58-59 22.206115567189688 27.32484478666085 28.30861617994122 22.160423466208236 60-61 22.485393944411808 27.42113793902564 28.270473609527926 21.822994507034625 62-63 22.36977723020782 27.433236720458503 28.301320315330454 21.895665734003224 64-65 22.241425096673233 27.086496272261506 28.27918709235852 22.392891538706735 66-67 22.13703077258839 27.186313291139243 28.21618561763422 22.460470318638148 68-69 21.971727663133795 27.269520490439774 28.284163270951662 22.474588575474765 70-71 22.075573918874166 27.08593738491479 28.29844108810119 22.540047608109855 72-73 21.896629613180814 27.552246615373033 27.921522048224457 22.6296017232217 74-75 22.051263305889098 27.475682364128673 27.823718212762195 22.649336117220034 76-77 21.92961877472814 27.29910329461213 28.076355260315168 22.694922670344564 78-79 21.813742105650107 27.36446529432297 28.04129825457818 22.780494345448744 80-81 21.866470872103285 27.500533997572663 27.78025870306925 22.8527364272548 82-83 22.01722116215153 27.48793165445054 27.815851806664206 22.678995376733724 84-85 21.957829878996353 27.665247791203228 27.614747756281538 22.762174573518884 86-87 21.752668565804978 27.641684385257875 27.752061227156933 22.85358582178021 88-89 21.632119086497863 27.420888428709212 27.865170991764508 23.081821493028414 90-91 21.96266304037168 27.485432452184217 27.790268783927452 22.76163572351665 92-93 21.81910461649079 27.556520001064193 27.677729062819555 22.946646319625458 94-95 21.593333852716206 27.457348410354133 27.690388501572677 23.258929235356984 96-97 21.55470408458238 27.47815545867637 27.75499190281026 23.212148553930994 98-99 21.50571268577057 27.372873899393163 27.626126866572587 23.495286548263685 100 20.63738302458189 27.975090715630373 27.87859111123237 23.50893514855537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1796.0 1 1334.0 2 2316.0 3 3452.5 4 2253.5 5 1501.0 6 2052.0 7 3755.0 8 5876.5 9 6937.5 10 6005.0 11 4678.5 12 4637.5 13 4857.5 14 5143.5 15 5576.0 16 5794.0 17 5961.5 18 6333.0 19 6838.5 20 7543.5 21 8413.0 22 9549.0 23 11265.5 24 13687.5 25 16739.5 26 20692.0 27 25193.0 28 30134.0 29 35905.0 30 42620.0 31 49603.5 32 57114.0 33 65025.0 34 72621.5 35 79687.0 36 88411.5 37 97220.5 38 103464.5 39 108698.5 40 112373.0 41 115367.5 42 118520.0 43 121865.0 44 124946.0 45 129037.0 46 132564.0 47 134981.5 48 138723.0 49 141634.5 50 142930.0 51 140209.0 52 133515.5 53 127316.0 54 120942.5 55 111244.0 56 99952.5 57 89796.5 58 80241.0 59 69217.0 60 56465.0 61 45137.5 62 36201.0 63 28097.0 64 20910.0 65 15538.0 66 11733.5 67 8984.5 68 6776.5 69 5076.5 70 3885.5 71 3042.0 72 2369.0 73 1853.5 74 1453.0 75 1034.0 76 734.5 77 507.5 78 330.0 79 204.0 80 132.0 81 101.5 82 69.5 83 47.0 84 34.5 85 25.0 86 17.0 87 13.0 88 8.0 89 4.5 90 4.5 91 2.0 92 0.5 93 1.0 94 2.0 95 3.0 96 2.0 97 0.5 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033279050292282807 2 0.06759466116743999 3 8.728931224205327E-4 4 7.63781482117966E-4 5 0.002209510716126973 6 4.910023813615496E-4 7 0.0 8 8.728931224205327E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.092256619666828E-4 18-19 0.0010365605828743826 20-21 0.00298693115328276 22-23 1.3638955037820824E-5 24-25 5.4555820151283295E-5 26-27 1.6366746045384987E-4 28-29 9.9564371776092E-4 30-31 0.0013638955037820822 32-33 0.002932375333131477 34-35 0.004037130691194963 36-37 0.0011593111782147699 38-39 0.001459368189046828 40-41 0.005414665150014866 42-43 0.006505781553040531 44-45 0.005114608139182809 46-47 0.006778560653796948 48-49 0.008510707943600194 50-51 0.008183373022692493 52-53 0.0038052684555520094 54-55 0.0022777054913160773 56-57 0.002673235187412881 58-59 0.0035052114447199517 60-61 0.007678731686293123 62-63 0.013147952656459273 64-65 0.0038598242757032926 66-67 0.010092826727987409 68-69 0.012097753118547069 70-71 0.0056738052957334625 72-73 0.007583259001028377 74-75 0.003055125928471864 76-77 0.0028914584680180145 78-79 0.008033344517276464 80-81 0.006082973946868087 82-83 0.01625763440508242 84-85 0.01707597170735167 86-87 0.021795050150437676 88-89 0.02004926390559661 90-91 0.024236423102207603 92-93 0.03348363461785012 94-95 0.03722070829821302 96-97 0.022081468206231913 98-99 0.01807161542511259 100 0.0182761997506799 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3665970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.1918058622312 #Duplication Level Percentage of deduplicated Percentage of total 1 78.63591216207428 52.83688943793561 2 14.360619724405199 19.298319451671247 3 3.698347798455414 7.454960018544791 4 1.3018790666996596 3.499024220231451 5 0.6047142195147005 2.031592021988121 6 0.3450841163534397 1.3912094971295954 7 0.22421505663982383 1.054579018999255 8 0.156663077067693 0.8421180048089755 9 0.11185453613814846 0.6764137449303956 >10 0.5192210728233668 5.96861117132093 >50 0.026759959013716472 1.2266744397891443 >100 0.013736891849463053 1.6833012469519875 >500 5.670394092719251E-4 0.2788949417481801 >1k 2.362664199642202E-4 0.3571912669950061 >5k 9.450656798568807E-5 0.46780943359150395 >10k+ 9.450656798568807E-5 0.9324120833637638 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15494 0.42264393871199163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13873 0.37842644647937657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9482 0.2586491433372341 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5252 0.14326358371726994 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.455582015128329E-5 0.0 5 0.0 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 6 0.0 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 7 0.0 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 8 0.0 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 9 0.0 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 10-11 2.7277910075641644E-5 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 12-13 2.7277910075641644E-5 2.7277910075641644E-5 0.0 5.455582015128329E-5 0.0 14-15 2.7277910075641644E-5 2.7277910075641644E-5 0.0 5.455582015128329E-5 1.3638955037820822E-5 16-17 2.7277910075641644E-5 2.7277910075641644E-5 0.0 6.819477518910411E-5 5.455582015128329E-5 18-19 2.7277910075641644E-5 2.7277910075641644E-5 0.0 1.0911164030256659E-4 5.455582015128329E-5 20-21 2.7277910075641644E-5 2.7277910075641644E-5 0.0 1.6366746045384987E-4 6.819477518910411E-5 22-23 2.7277910075641644E-5 2.7277910075641644E-5 0.0 2.1822328060513315E-4 9.547268526474576E-5 24-25 5.455582015128329E-5 2.7277910075641644E-5 0.0 3.81890741058983E-4 1.0911164030256658E-4 26-27 5.455582015128329E-5 2.7277910075641644E-5 0.0 6.001140216641161E-4 1.0911164030256658E-4 28-29 5.455582015128329E-5 2.7277910075641644E-5 0.0 0.001295700728592978 1.0911164030256658E-4 30-31 5.455582015128329E-5 2.7277910075641644E-5 0.0 0.0043235487469892005 1.0911164030256658E-4 32-33 5.455582015128329E-5 2.7277910075641644E-5 0.0 0.01160675073718552 1.0911164030256658E-4 34-35 5.455582015128329E-5 6.819477518910411E-5 0.0 0.022463358947290894 1.6366746045384987E-4 36-37 5.455582015128329E-5 1.5002850541602904E-4 0.0 0.04215801002190416 1.6366746045384987E-4 38-39 5.455582015128329E-5 2.0458432556731232E-4 0.0 0.07313207691279525 1.6366746045384987E-4 40-41 5.455582015128329E-5 2.3186223564295399E-4 0.0 0.11152573534426086 1.6366746045384987E-4 42-43 5.455582015128329E-5 2.7277910075641646E-4 0.0 0.14765532723944824 1.773064154916707E-4 44-45 5.455582015128329E-5 2.7277910075641646E-4 0.0 0.19383682899750954 1.909453705294915E-4 46-47 5.455582015128329E-5 2.7277910075641646E-4 0.0 0.24930645913632682 1.909453705294915E-4 48-49 5.455582015128329E-5 2.8641805579423727E-4 0.0 0.3124957378265507 1.909453705294915E-4 50-51 5.455582015128329E-5 3.000570108320581E-4 0.0 0.3829273016418574 2.0458432556731232E-4 52-53 5.455582015128329E-5 3.000570108320581E-4 0.0 0.45503645692681605 2.3186223564295399E-4 54-55 5.455582015128329E-5 3.136959658698789E-4 0.0 0.5409482347100495 2.455011906807748E-4 56-57 5.455582015128329E-5 3.2733492090769974E-4 0.0 0.6342523261237818 2.455011906807748E-4 58-59 5.455582015128329E-5 3.2733492090769974E-4 0.0 0.7429275198651379 2.455011906807748E-4 60-61 5.455582015128329E-5 3.2733492090769974E-4 0.0 0.8598270034943003 2.455011906807748E-4 62-63 5.455582015128329E-5 3.5461283098334135E-4 0.0 0.9844188577647934 2.7277910075641646E-4 64-65 5.455582015128329E-5 3.682517860211622E-4 0.0 1.1115748355823971 2.7277910075641646E-4 66-67 5.455582015128329E-5 3.81890741058983E-4 0.0 1.2584663813397272 2.7277910075641646E-4 68-69 5.455582015128329E-5 3.81890741058983E-4 0.0 1.433713314620687 2.7277910075641646E-4 70-71 5.455582015128329E-5 3.81890741058983E-4 0.0 1.6240312932184389 2.7277910075641646E-4 72-73 5.455582015128329E-5 3.81890741058983E-4 0.0 1.817895400126024 3.000570108320581E-4 74-75 5.455582015128329E-5 3.81890741058983E-4 0.0 2.018251649631612 3.000570108320581E-4 76-77 5.455582015128329E-5 3.81890741058983E-4 0.0 2.235370174878682 3.000570108320581E-4 78-79 5.455582015128329E-5 3.81890741058983E-4 0.0 2.4854676934072018 3.000570108320581E-4 80-81 5.455582015128329E-5 3.81890741058983E-4 0.0 2.7544278867530285 3.136959658698789E-4 82-83 5.455582015128329E-5 3.81890741058983E-4 0.0 3.0396593534589753 3.2733492090769974E-4 84-85 5.455582015128329E-5 3.81890741058983E-4 0.0 3.331123822617206 3.2733492090769974E-4 86-87 5.455582015128329E-5 3.81890741058983E-4 0.0 3.6399097646734697 3.2733492090769974E-4 88 5.455582015128329E-5 3.81890741058983E-4 0.0 3.8867748508580267 3.2733492090769974E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5000 0.0 49.087257 2 GTACCTG 5405 0.0 46.45364 1 ACCTGGG 5540 0.0 43.17687 3 TATAACG 460 0.0 42.929886 2 GGTATCA 8065 0.0 42.500824 1 TAACGCA 475 0.0 38.584084 4 GTATCAA 12905 0.0 35.3053 1 ATAACGC 530 0.0 34.580544 3 CCTGGGG 5135 0.0 31.11542 4 TCAACGC 16020 0.0 28.014187 4 ATCAACG 16045 0.0 27.824474 3 CAACGCA 16390 0.0 27.35273 5 TATCAAC 16630 0.0 26.831331 2 AACGCAG 16980 0.0 26.40159 6 GTACATG 13350 0.0 23.632814 1 ACGCAGA 19115 0.0 23.403248 7 CGCAGAG 19350 0.0 23.046167 8 TACATGG 13520 0.0 22.778906 2 CATGGGG 7705 0.0 22.688587 4 ACATGGG 13005 0.0 22.295536 3 >>END_MODULE