FastQCFastQC Report
Mon 6 Jun 2016
ERR1211485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119329
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183790.3590118939415693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165080.3224641354364996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104130.2034055635025606No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52900.10333385488606026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90800.048.493221
TACCTGG55200.037.8246082
GTATCAA153150.036.5919231
GTACCTG58650.036.2932321
ACCTGGG67850.030.8192673
TCAACGC184150.029.957124
ATCAACG186000.029.6344743
TATAACG5400.029.6084522
CAACGCA186900.029.5640225
AACGCAG193700.028.6663726
TATCAAC196550.028.3036372
GTACATG172550.025.7912331
TAACGCA5900.025.4859984
ACGCAGA220350.025.0506027
TACATGG176150.024.880772
ATAACGC6350.024.4203783
CGCAGAG225600.024.3644778
ACATGGG174100.023.8327473
CATGGGG102100.022.5514284
GCAGAGT246400.021.5285369
Produced by FastQC (version 0.11.5)