FastQCFastQC Report
Mon 6 Jun 2016
ERR1211485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119329
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201130.392883520477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183310.35807427106169576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125570.24528605213691093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102200.045.780781
TACCTGG52950.039.429732
GTACCTG56150.037.685461
GTATCAA166000.035.2106551
ACCTGGG65050.031.497383
TCAACGC207700.027.671024
ATCAACG209600.027.3305053
CAACGCA210200.027.274585
AACGCAG217700.026.4852686
TATCAAC220800.026.1307752
TAACGCA4950.024.6833084
GTACATG188350.023.5427461
ACGCAGA247350.023.2154717
TACATGG189500.022.8537352
CGCAGAG251450.022.687438
CATGGGG104400.022.1912424
ACATGGG190400.021.7442383
TATAACG5000.020.689952
CCTGGGG62050.020.5997684
GAGTACT163700.020.4098212-13
Produced by FastQC (version 0.11.5)