##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5119329 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.077814104153106 33.0 31.0 34.0 30.0 34.0 2 32.295978633137274 34.0 31.0 34.0 30.0 34.0 3 32.3069074091546 34.0 31.0 34.0 30.0 34.0 4 35.95837188819082 37.0 35.0 37.0 35.0 37.0 5 35.8806355286015 37.0 35.0 37.0 35.0 37.0 6 35.89893109038314 37.0 35.0 37.0 35.0 37.0 7 35.82424649792971 37.0 35.0 37.0 35.0 37.0 8 35.8169490571909 37.0 35.0 37.0 35.0 37.0 9 37.467569284959026 39.0 37.0 39.0 34.0 39.0 10-11 37.42084411843818 39.0 37.0 39.0 34.0 39.0 12-13 37.33547970837584 39.0 37.0 39.0 34.0 39.0 14-15 38.55779048387005 40.0 38.0 41.0 34.0 41.0 16-17 38.48317631080167 40.0 38.0 41.0 34.0 41.0 18-19 38.47505864538107 40.0 38.0 41.0 34.0 41.0 20-21 38.41199178642357 40.0 38.0 41.0 34.0 41.0 22-23 38.30101034725449 40.0 38.0 41.0 34.0 41.0 24-25 38.186922348612484 40.0 38.0 41.0 33.0 41.0 26-27 38.00434617505536 40.0 37.0 41.0 33.0 41.0 28-29 38.0329909447117 40.0 37.0 41.0 33.0 41.0 30-31 37.949942756169804 40.0 37.0 41.0 33.0 41.0 32-33 37.752409544297706 40.0 37.0 41.0 33.0 41.0 34-35 37.63199102851175 40.0 37.0 41.0 33.0 41.0 36-37 37.5633625031718 40.0 37.0 41.0 32.0 41.0 38-39 37.46456215257898 40.0 36.5 41.0 32.0 41.0 40-41 37.298734170044554 40.0 36.0 41.0 31.0 41.0 42-43 37.14039056681061 39.5 36.0 41.0 31.0 41.0 44-45 36.951929637653684 39.0 36.0 41.0 30.5 41.0 46-47 36.687133216091404 39.0 35.0 41.0 30.0 41.0 48-49 36.45812068339425 39.0 35.0 41.0 29.5 41.0 50-51 36.251831538859875 39.0 35.0 40.0 29.0 41.0 52-53 36.052739528949985 39.0 35.0 40.0 28.0 41.0 54-55 35.855130721233195 38.5 35.0 40.0 28.0 41.0 56-57 35.64922063028182 38.0 34.0 40.0 27.0 41.0 58-59 35.388493296680096 38.0 34.0 40.0 26.5 41.0 60-61 35.071374783687475 38.0 34.0 40.0 26.0 41.0 62-63 34.73857540705042 37.0 33.5 40.0 26.0 41.0 64-65 34.42349378209527 37.0 33.0 39.5 26.0 41.0 66-67 34.07995393536926 36.0 33.0 39.0 25.0 40.5 68-69 33.67964395333841 36.0 32.0 39.0 24.5 40.0 70-71 33.288619270220764 35.0 32.0 38.5 24.0 40.0 72-73 32.87914910332975 35.0 31.0 37.5 23.0 40.0 74-75 32.469528037756504 35.0 31.0 37.0 22.0 39.0 76-77 30.931792428265503 33.5 29.5 35.5 20.5 38.0 78-79 31.77066008064729 34.0 30.5 36.0 22.0 38.5 80-81 31.74779214229052 34.0 31.0 36.0 22.0 37.5 82-83 31.46325436399966 34.0 30.5 35.0 20.5 37.0 84-85 31.164744344424825 34.0 30.0 35.0 20.0 37.0 86-87 30.87011110245112 34.0 30.0 35.0 20.0 36.0 88-89 30.59598445421265 34.0 29.5 35.0 19.5 36.0 90-91 30.3091231292226 34.0 29.0 35.0 18.5 35.5 92-93 30.088320949874486 34.0 29.0 35.0 18.0 35.0 94-95 29.874117291543484 34.0 29.0 35.0 18.0 35.0 96-97 29.663536861959837 34.0 29.0 35.0 17.5 35.0 98-99 29.500115640155183 33.0 29.0 35.0 17.0 35.0 100 27.623016414846553 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27040536943665217 1101 2 0.18122897555602435 1101 3 0.25603411244768637 1101 4 0.1718278783697329 1101 5 0.1630385565453949 1101 6 0.15589431837824463 1101 7 0.21340536661816145 1101 8 0.17766721099789606 1101 9 0.13567756181652868 1101 10-11 0.24541093153594318 1101 12-13 0.25178636401533794 1101 14-15 0.3156539535712142 1101 16-17 0.33679016458793853 1101 18-19 0.34072965167268876 1101 20-21 0.2804516493178184 1101 22-23 0.19593322498989352 1101 24-25 0.2429711583466414 1101 26-27 0.2075179817017485 1101 28-29 0.2880723723712961 1101 30-31 0.29394481874897593 1101 32-33 0.3562171935748353 1101 34-35 0.3668109373003503 1101 36-37 0.3580760317410565 1101 38-39 0.33852825974725675 1101 40-41 0.4103627226754796 1101 42-43 0.3192198304852667 1101 44-45 0.3606604557507822 1101 46-47 0.41469939991930715 1101 48-49 0.41941864812657315 1101 50-51 0.37049593737703646 1101 52-53 0.2942701012970659 1101 54-55 0.31957601860247564 1101 56-57 0.31887396641431565 1101 58-59 0.41311722560046604 1101 60-61 0.30983677413581745 1101 62-63 0.23322065183712226 1101 64-65 0.20448754274435998 1101 66-67 0.18616190407112754 1101 68-69 0.18056915364615378 1101 70-71 0.25533583516032365 1101 72-73 0.13411585296527306 1101 74-75 0.12230806931599858 1101 76-77 0.2600191547421531 1101 78-79 0.33305526969775556 1101 80-81 0.29492664214077635 1101 82-83 0.2968044424757501 1101 84-85 0.3088905777937896 1101 86-87 0.2643490763230609 1101 88-89 0.18993162916888195 1101 90-91 0.07436666871037545 1101 92-93 -0.0476974085817794 1101 94-95 0.11482234000661862 1101 96-97 -0.059283873767270734 1101 98-99 -0.11362039430530047 1101 100 -0.012724157330918473 1102 1 0.3032676216228012 1102 2 0.23776441544674753 1102 3 0.3190992514718758 1102 4 0.1776417166307951 1102 5 0.23129330931448067 1102 6 0.1735673744692221 1102 7 0.194567638022356 1102 8 0.1945426639975878 1102 9 0.2681139191650246 1102 10-11 0.2147013477385329 1102 12-13 0.24443229213859752 1102 14-15 0.35423014713629897 1102 16-17 0.31496434486560076 1102 18-19 0.2985769894485202 1102 20-21 0.3364106863562597 1102 22-23 0.3217603294089528 1102 24-25 0.2934234760791341 1102 26-27 0.35575840492424504 1102 28-29 0.3643873332778895 1102 30-31 0.41745120855856754 1102 32-33 0.44297913061267735 1102 34-35 0.4083546323229683 1102 36-37 0.4853345034420542 1102 38-39 0.4569945883179969 1102 40-41 0.4854941628997125 1102 42-43 0.42123823450543796 1102 44-45 0.442829679096441 1102 46-47 0.4978799982865354 1102 48-49 0.5089082077311247 1102 50-51 0.4412220360616601 1102 52-53 0.4246791930537839 1102 54-55 0.4172623161931597 1102 56-57 0.43663953731297056 1102 58-59 0.4529584200725125 1102 60-61 0.40418261368958497 1102 62-63 0.484568851342992 1102 64-65 0.5219516198594576 1102 66-67 0.4497538365579743 1102 68-69 0.406359994973009 1102 70-71 0.41134782586846086 1102 72-73 0.4150109364266399 1102 74-75 0.34016231757654936 1102 76-77 0.4203868313659882 1102 78-79 0.42918411910668297 1102 80-81 0.36893915098115926 1102 82-83 0.43590405440055946 1102 84-85 0.3628853049275058 1102 86-87 0.3286657928996384 1102 88-89 0.35515897625629833 1102 90-91 0.2691276613358511 1102 92-93 0.1817737436120872 1102 94-95 0.26344519783726383 1102 96-97 0.37754749946347843 1102 98-99 0.29391208027239557 1102 100 0.2218926860354884 1103 1 0.2595542327532243 1103 2 0.24014612876310792 1103 3 0.29607083329815964 1103 4 0.11460051474224286 1103 5 0.1911921729470336 1103 6 0.18983126982799803 1103 7 0.18640010555047581 1103 8 0.20449373186126252 1103 9 0.1824058747169417 1103 10-11 0.19797580976989337 1103 12-13 0.22162940172725598 1103 14-15 0.29514111173095614 1103 16-17 0.3457791604477194 1103 18-19 0.3061673506006457 1103 20-21 0.31282375755235137 1103 22-23 0.38849531197148934 1103 24-25 0.28940982833628937 1103 26-27 0.34951444679852983 1103 28-29 0.3475506842319902 1103 30-31 0.47605626945944124 1103 32-33 0.4688283290865485 1103 34-35 0.452625191299731 1103 36-37 0.46879933497334747 1103 38-39 0.42222305152109385 1103 40-41 0.5457446700896398 1103 42-43 0.5811757600401108 1103 44-45 0.539807850811961 1103 46-47 0.513829723799212 1103 48-49 0.5470904615829184 1103 50-51 0.47246336645762455 1103 52-53 0.44926101842568045 1103 54-55 0.583257238657616 1103 56-57 0.546279649539116 1103 58-59 0.5738649274281755 1103 60-61 0.5882659747219847 1103 62-63 0.6728760632653632 1103 64-65 0.5856588941161434 1103 66-67 0.5991791634856369 1103 68-69 0.549777448156 1103 70-71 0.596904056086224 1103 72-73 0.5726225022153955 1103 74-75 0.5240997748664213 1103 76-77 0.5002040693341101 1103 78-79 0.6222304302158683 1103 80-81 0.5487367076342515 1103 82-83 0.4719496059815995 1103 84-85 0.47463637528942826 1103 86-87 0.4200295039060329 1103 88-89 0.42588521373421173 1103 90-91 0.40692424062580557 1103 92-93 0.5030254661079816 1103 94-95 0.5938898158049675 1103 96-97 0.5738668498216555 1103 98-99 0.6274117958682552 1103 100 0.5104004432460556 1104 1 0.22594210041906138 1104 2 0.14886370582381403 1104 3 0.23802153640917822 1104 4 0.0704648755480548 1104 5 0.11938799370139463 1104 6 0.14772246420661617 1104 7 0.20423376594618503 1104 8 0.18900079120623303 1104 9 0.20835143502087305 1104 10-11 0.22677644261246144 1104 12-13 0.2555310602597203 1104 14-15 0.30557640005747544 1104 16-17 0.2692767811004586 1104 18-19 0.2938473936039543 1104 20-21 0.2123126607601904 1104 22-23 0.3240324345826906 1104 24-25 0.3130958587858288 1104 26-27 0.33112330053911165 1104 28-29 0.38867767194242475 1104 30-31 0.3381505768439297 1104 32-33 0.2755534468644498 1104 34-35 0.41661962437085265 1104 36-37 0.32365386540504915 1104 38-39 0.4579939096924832 1104 40-41 0.45348592600196014 1104 42-43 0.5280235679612417 1104 44-45 0.5551750567781113 1104 46-47 0.5118802053456477 1104 48-49 0.4362334063650266 1104 50-51 0.41190874732078697 1104 52-53 0.41800531515906414 1104 54-55 0.42144080914070514 1104 56-57 0.40578239858121634 1104 58-59 0.4808296680092141 1104 60-61 0.3685061586154177 1104 62-63 0.4085519806329856 1104 64-65 0.5133763463432715 1104 66-67 0.48346909770590685 1104 68-69 0.6041738009008597 1104 70-71 0.5444841048686442 1104 72-73 0.5605720067318103 1104 74-75 0.5640199979184288 1104 76-77 0.48741808899822203 1104 78-79 0.6188702862980087 1104 80-81 0.7505399821668455 1104 82-83 0.5524437463125658 1104 84-85 0.5649474632146791 1104 86-87 0.5918974696269039 1104 88-89 0.614102593812575 1104 90-91 0.40216104055602386 1104 92-93 0.5058878977003225 1104 94-95 0.6117121222321167 1104 96-97 0.5001618420246672 1104 98-99 0.5744592293847575 1104 100 0.6762657039590039 1105 1 0.22703568817998132 1105 2 0.17760974722092016 1105 3 0.19881772165980038 1105 4 0.10079968192960109 1105 5 0.12078736502458298 1105 6 0.1180886285486693 1105 7 0.13137950127824638 1105 8 0.15476777984824253 1105 9 0.17865095971927047 1105 10-11 0.18569034859879707 1105 12-13 0.19017314356796078 1105 14-15 0.2459765096181883 1105 16-17 0.2577950887056204 1105 18-19 0.27314538136820943 1105 20-21 0.24999083154664703 1105 22-23 0.2804762678271757 1105 24-25 0.3096995929067461 1105 26-27 0.27726583202802857 1105 28-29 0.28177061056346275 1105 30-31 0.29978587698301595 1105 32-33 0.34832210192590907 1105 34-35 0.47670685927479894 1105 36-37 0.4674272137576949 1105 38-39 0.4141071760361541 1105 40-41 0.42998712930953786 1105 42-43 0.35717448343940816 1105 44-45 0.4177006233925411 1105 46-47 0.4707441163008923 1105 48-49 0.4873910514069095 1105 50-51 0.4323263630975376 1105 52-53 0.44852079513828613 1105 54-55 0.381643081570914 1105 56-57 0.3982871520867377 1105 58-59 0.45366471182126133 1105 60-61 0.42782795551883623 1105 62-63 0.43505647614701815 1105 64-65 0.38413974170715903 1105 66-67 0.3933685972905252 1105 68-69 0.5197526625855957 1105 70-71 0.5440378823879399 1105 72-73 0.5545923378812745 1105 74-75 0.46846162501743294 1105 76-77 0.4447780490609432 1105 78-79 0.6345678693533934 1105 80-81 0.6111397609893139 1105 82-83 0.6481467498818567 1105 84-85 0.6157386820066435 1105 86-87 0.539215557474229 1105 88-89 0.5670767902571896 1105 90-91 0.6844853676853226 1105 92-93 0.6482817014006166 1105 94-95 0.6506362524164864 1105 96-97 0.6640678895509069 1105 98-99 0.6927169068346863 1105 100 0.7894401962860833 1106 1 0.25258999546272776 1106 2 0.1886928648873223 1106 3 0.22553286086075985 1106 4 0.14840707664829011 1106 5 0.13396813717401557 1106 6 0.17502624457006277 1106 7 0.19765197327861728 1106 8 0.1755162368100187 1106 9 0.19072694863560002 1106 10-11 0.21052266601108727 1106 12-13 0.19113397375589614 1106 14-15 0.26705075342441376 1106 16-17 0.24154439968374675 1106 18-19 0.24037132987015752 1106 20-21 0.27506810704910123 1106 22-23 0.2726268873907003 1106 24-25 0.24731255541654207 1106 26-27 0.227435306690559 1106 28-29 0.3279725860478848 1106 30-31 0.36771475138699117 1106 32-33 0.37394704167511605 1106 34-35 0.40312185684005186 1106 36-37 0.44149774487923565 1106 38-39 0.3412187036501706 1106 40-41 0.37015756798474087 1106 42-43 0.36675389583660944 1106 44-45 0.4626520087281847 1106 46-47 0.42218868310078506 1106 48-49 0.4978725262968524 1106 50-51 0.5015990155413093 1106 52-53 0.5385920517411549 1106 54-55 0.42927763549259623 1106 56-57 0.47463748046899923 1106 58-59 0.5307030115501092 1106 60-61 0.6730018053157778 1106 62-63 0.6670769910780834 1106 64-65 0.7380794637254553 1106 66-67 0.6411882292848716 1106 68-69 0.6575630005091071 1106 70-71 0.6411142050009602 1106 72-73 0.6033885270650501 1106 74-75 0.6241132728611305 1106 76-77 0.6505383646144196 1106 78-79 0.6622861996834679 1106 80-81 0.69318020502838 1106 82-83 0.5981139672716544 1106 84-85 0.5752238868840536 1106 86-87 0.5411833792258172 1106 88-89 0.6068302007609674 1106 90-91 0.6357232588211019 1106 92-93 0.5847570980671115 1106 94-95 0.669352342035058 1106 96-97 0.8098329695516 1106 98-99 0.9273692999343339 1106 100 0.934999249477336 1107 1 0.26807791875842213 1107 2 0.185209935071164 1107 3 0.25374003643893417 1107 4 0.16075352762737083 1107 5 0.13089299341201865 1107 6 0.1726211616815334 1107 7 0.2089889321508025 1107 8 0.21187058516125745 1107 9 0.22822800530171605 1107 10-11 0.17451791188423726 1107 12-13 0.2541709857225811 1107 14-15 0.2806995077931447 1107 16-17 0.2486885865637376 1107 18-19 0.20661289751625844 1107 20-21 0.2660700449826763 1107 22-23 0.3766145790905142 1107 24-25 0.32452280806976574 1107 26-27 0.3520622125283879 1107 28-29 0.41225827500526435 1107 30-31 0.3480118304927373 1107 32-33 0.354590406492612 1107 34-35 0.40025369299490876 1107 36-37 0.475831003599545 1107 38-39 0.49659239302800273 1107 40-41 0.3918802962634018 1107 42-43 0.40167643389953867 1107 44-45 0.39036740318994845 1107 46-47 0.3700790867994783 1107 48-49 0.39244202231698466 1107 50-51 0.3645358064866784 1107 52-53 0.4005342468015627 1107 54-55 0.3967697417613891 1107 56-57 0.4898137703694303 1107 58-59 0.5390848354365474 1107 60-61 0.47121012700071674 1107 62-63 0.414839217615274 1107 64-65 0.49672022225817614 1107 66-67 0.5674059123192308 1107 68-69 0.5580527236989994 1107 70-71 0.5573773017244505 1107 72-73 0.5294082850592261 1107 74-75 0.5593795638031835 1107 76-77 0.47316583654034616 1107 78-79 0.616663884288176 1107 80-81 0.6845060605955062 1107 82-83 0.7080057868238008 1107 84-85 0.5865996297559484 1107 86-87 0.43725410097863815 1107 88-89 0.45597283691418156 1107 90-91 0.3890455120670069 1107 92-93 0.4865391357879538 1107 94-95 0.7302387258117555 1107 96-97 0.7454718876648059 1107 98-99 0.7600849492623887 1107 100 0.9432279161404153 1108 1 0.26424130864005235 1108 2 0.19506900024015295 1108 3 0.28815232106223476 1108 4 0.20215639566742993 1108 5 0.15966381490770942 1108 6 0.17898624444694633 1108 7 0.17848878718078964 1108 8 0.1976129499424104 1108 9 0.23694100284937036 1108 10-11 0.24992060198503907 1108 12-13 0.26672977429699074 1108 14-15 0.2984348143171758 1108 16-17 0.30628359555692697 1108 18-19 0.366432967486773 1108 20-21 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82-83 -0.301927728125829 2316 84-85 -0.349153516781449 2316 86-87 -0.3351372606881178 2316 88-89 -0.25691968882038907 2316 90-91 -0.16036851456161116 2316 92-93 -0.12394471490810233 2316 94-95 -0.010659675468438934 2316 96-97 -0.05869811313119655 2316 98-99 -0.17658856563047465 2316 100 -0.23121251435993173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 28.0 12 55.0 13 225.0 14 560.0 15 1495.0 16 3451.0 17 7167.0 18 12845.0 19 19150.0 20 26279.0 21 35092.0 22 44760.0 23 51218.0 24 54448.0 25 59410.0 26 68697.0 27 81455.0 28 97909.0 29 118160.0 30 141781.0 31 170958.0 32 209205.0 33 261997.0 34 344163.0 35 465389.0 36 674918.0 37 1035458.0 38 988415.0 39 144627.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35945719134732 20.04285084717156 13.884662928547542 23.71302903293358 2 15.261859035443942 21.462227499963348 39.71787541598862 23.558038048604086 3 17.169633156446963 27.254207318431018 30.937996528427554 24.638162996694465 4 12.005622651409091 17.750810512310977 38.08836493633008 32.15520189994986 5 12.336921689398217 38.03493993154847 35.47631944801214 14.151818931041172 6 30.145295170065147 37.886036438944906 18.430751217499726 13.537917173490218 7 26.441473091493044 32.98727235542002 23.095663513714396 17.475591039372542 8 25.655176293655874 35.87954222388305 20.745629602339864 17.719651880121212 9 26.546545455468873 15.130811088718854 20.60445812332046 37.718185332491814 10-11 24.30939679790066 26.426812967090026 29.345623225231275 19.91816700977804 12-13 24.952000545383974 24.388030150045054 29.057636264440124 21.602333040130844 14-15 22.428955044694334 25.70769528584703 26.79741817726503 25.065931492193606 16-17 21.894066971383342 28.41899395834306 27.448000123454598 22.238938946819005 18-19 21.696709338081924 27.425568547415125 29.500862817657346 21.37685929684561 20-21 22.554128223876173 26.790298928325925 29.41684880821896 21.23872403957894 22-23 22.278105196989685 26.71363766618633 29.174634019419344 21.833623117404642 24-25 22.011611876755296 26.823532973813457 29.30492335174884 21.85993179768241 26-27 21.97435090538339 26.909251477197277 29.252031958131962 21.864365659287373 28-29 21.948770620295125 26.994842447847738 29.360561593611926 21.695825338245214 30-31 22.369897934267716 26.872295746447232 29.30704693070274 21.45075938858231 32-33 22.08135263454508 26.909435956239214 29.301198723627447 21.708012685588262 34-35 22.256636697064728 27.13162379204363 29.13614350402993 21.47559600686171 36-37 22.305876768502525 27.057904280251034 29.023693331125987 21.612525620120458 38-39 22.204954111558635 27.2233131995758 28.973506537952826 21.59822615091274 40-41 22.31331404533029 26.99352802554135 29.06465557279284 21.62850235633552 42-43 22.142586302652223 27.12037391743803 28.83699150867817 21.900048271231576 44-45 22.090080319182604 27.290227767667446 28.79808777990403 21.821604133245923 46-47 22.09092118493068 27.106341240047094 28.617139381005952 22.185598194016272 48-49 22.109965667291053 27.250211691836075 28.58218287810838 22.057639762764488 50-51 22.134781766125304 27.45765115712089 28.450205824987812 21.957361251766 52-53 22.163404822568793 27.497758900471347 28.238293588042833 22.100542688917024 54-55 22.0876414750925 27.453290596449875 28.395150717446555 22.063917211011066 56-57 22.189011770382315 27.52284778593551 28.091246590046698 22.196893853635476 58-59 22.121839058455457 27.488829716889263 28.31010655452854 22.079224670126738 60-61 22.374777824021876 27.562646129207792 28.23209701401932 21.83047903275101 62-63 22.188491444643795 27.540786992939818 28.32684609013476 21.943875472281626 64-65 22.11828765361835 27.326838817344207 28.28954944478462 22.265324084252832 66-67 22.081880975788913 27.37719978139547 28.181113564470024 22.359805678345598 68-69 22.008692632556787 27.443859063893633 28.243710198043352 22.30373810550623 70-71 22.034820914036786 27.28531661148065 28.22794268467782 22.45191978980475 72-73 21.981062928090385 27.562359419522036 27.911366267405413 22.545211384982167 74-75 21.99262734348781 27.604320296234597 27.819881910913182 22.583170449364413 76-77 21.87463678328735 27.472652406465166 28.032819261019664 22.619891549227816 78-79 21.88816644333009 27.451762376133175 28.009493342929044 22.650577837607692 80-81 21.844168407390526 27.62569302558547 27.806205422541225 22.723933144482782 82-83 21.988059996004694 27.559391092032197 27.832156150987373 22.620392760975733 84-85 21.919255306387438 27.69407520317658 27.6679931088567 22.718676381579282 86-87 21.784193065889205 27.601971829692545 27.851737835862107 22.762097268556143 88-89 21.741714523740747 27.366662443241413 27.884248580846076 23.00737445217176 90-91 22.036506367068128 27.43846903148604 27.774727054126696 22.750297547319136 92-93 21.842038825427828 27.440013568929338 27.73848507124665 22.97946253439618 94-95 21.624793644242672 27.32847850148201 27.79838729849048 23.248340555784843 96-97 21.676487885318384 27.346307371580075 27.783827031927427 23.19337771117411 98-99 21.580919865290262 27.2747262945298 27.694573268567158 23.44978057161278 100 20.739195120354875 27.717873048342206 27.968107252161563 23.574824579141353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2125.0 1 1616.0 2 2376.5 3 3339.0 4 2293.0 5 1801.5 6 2593.5 7 4839.5 8 7553.5 9 8638.5 10 7303.0 11 5726.0 12 5688.0 13 6066.5 14 6457.0 15 6821.5 16 7281.5 17 7770.5 18 8317.0 19 8916.0 20 9729.0 21 11078.5 22 13006.5 23 15790.0 24 19307.0 25 23711.5 26 29402.0 27 35883.5 28 43232.0 29 51789.5 30 61593.0 31 71461.5 32 81877.5 33 93924.0 34 105890.0 35 116335.5 36 128501.0 37 140920.0 38 149866.5 39 157420.0 40 162413.5 41 165014.0 42 167866.0 43 172409.0 44 177984.5 45 183473.5 46 187340.0 47 189670.5 48 192394.5 49 194577.5 50 195875.0 51 191931.0 52 181967.0 53 172684.0 54 163917.5 55 151794.0 56 137684.0 57 123041.5 58 107813.0 59 91921.5 60 75932.0 61 61667.0 62 49683.0 63 38711.0 64 28730.5 65 21392.0 66 16061.0 67 11985.5 68 9013.5 69 6794.5 70 5059.0 71 3853.0 72 3020.0 73 2317.5 74 1774.5 75 1357.0 76 979.5 77 655.5 78 449.5 79 304.0 80 196.0 81 128.5 82 90.0 83 62.5 84 36.5 85 30.5 86 26.0 87 17.5 88 11.5 89 9.5 90 5.5 91 3.0 92 3.0 93 3.0 94 2.0 95 1.5 96 2.0 97 2.0 98 2.0 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034574843695335854 2 0.06844647023076657 3 6.836833499077711E-4 4 7.03217159905136E-4 5 0.0016799076597733805 6 5.078790599314871E-4 7 0.0 8 0.0011329609798471636 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.422847798998658E-4 18-19 0.0010743595498550688 20-21 0.002773801019625814 22-23 0.0 24-25 2.9300714996047334E-5 26-27 1.1720285998418934E-4 28-29 7.813523998945955E-4 30-31 0.0015431709897918264 32-33 0.002617530539646895 34-35 0.0038579274744795656 36-37 0.0012013293148379406 38-39 0.0012892314598260826 40-41 0.005029956074321459 42-43 0.006602427779109333 44-45 0.004942053929333317 46-47 0.006758698259088252 48-49 0.008780447593815518 50-51 0.008145598768901158 52-53 0.003711423899499329 54-55 0.0019045464747430767 56-57 0.0024221924396732463 58-59 0.0032426124595625718 60-61 0.006983337074057948 62-63 0.013546697233172552 64-65 0.0037016569945006465 66-67 0.009698536663691668 68-69 0.012237931963349102 70-71 0.005449932989264804 72-73 0.0073251787490118325 74-75 0.0027933348296231794 76-77 0.0027347333996310843 78-79 0.007295878034015786 80-81 0.005664804899235818 82-83 0.016047024912835255 84-85 0.017092083747694276 86-87 0.022239242681999925 88-89 0.020520267402231816 90-91 0.02413402225174432 92-93 0.03428183654537538 94-95 0.038149530924853625 96-97 0.022639685786945904 98-99 0.01790273686258492 100 0.018049240437565155 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5119329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.99036283216597 #Duplication Level Percentage of deduplicated Percentage of total 1 76.96669396918226 50.02093367049436 2 15.11522942037972 19.64688488643815 3 4.159296930892962 8.10942649996344 4 1.5125278101496966 3.9319892470148097 5 0.7207603051591207 2.342123687365696 6 0.39155597224303834 1.5268418823105943 7 0.24481089820521923 1.1137244369737953 8 0.16479995027383676 0.8568326850415647 9 0.12082306365136893 0.7067101270676714 >10 0.5540389347509412 6.174425936611409 >50 0.0314198647752265 1.4074684560527917 >100 0.01661147388084978 1.9836870852989024 >500 9.417148856941506E-4 0.3976196754642814 >1k 3.3901724089755646E-4 0.40799948478049053 >5k 3.7668582321928774E-5 0.12477939361127438 >10k+ 1.1300574696578631E-4 1.2485528455108112 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20113 0.392883520477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18331 0.35807427106169576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12557 0.24528605213691093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.953380999736489E-5 2 0.0 0.0 0.0 1.953380999736489E-5 1.953380999736489E-5 3 0.0 0.0 0.0 1.953380999736489E-5 1.953380999736489E-5 4 0.0 0.0 0.0 1.953380999736489E-5 1.953380999736489E-5 5 0.0 0.0 0.0 1.953380999736489E-5 1.953380999736489E-5 6 0.0 0.0 0.0 1.953380999736489E-5 5.860142999209467E-5 7 0.0 0.0 0.0 1.953380999736489E-5 7.813523998945956E-5 8 0.0 0.0 0.0 1.953380999736489E-5 7.813523998945956E-5 9 0.0 0.0 0.0 1.953380999736489E-5 7.813523998945956E-5 10-11 0.0 0.0 0.0 1.953380999736489E-5 7.813523998945956E-5 12-13 0.0 0.0 0.0 1.953380999736489E-5 7.813523998945956E-5 14-15 0.0 0.0 0.0 1.953380999736489E-5 9.766904998682446E-5 16-17 0.0 0.0 0.0 1.953380999736489E-5 1.1720285998418934E-4 18-19 0.0 0.0 0.0 3.906761999472978E-5 1.3673666998155422E-4 20-21 0.0 0.0 0.0 3.906761999472978E-5 1.5627047997891912E-4 22-23 0.0 0.0 0.0 6.836833499077711E-5 1.6603738497760156E-4 24-25 0.0 0.0 0.0 1.074359549855069E-4 1.8557119497496645E-4 26-27 0.0 0.0 0.0 3.027740549591558E-4 2.0510500497233133E-4 28-29 0.0 0.0 0.0 0.0012013293148379406 2.1487190997101377E-4 30-31 0.0 0.0 0.0 0.004580678444382067 2.246388149696962E-4 32-33 0.0 0.0 0.0 0.012325834108337246 2.3440571996837867E-4 34-35 0.0 0.0 0.0 0.024593066786682397 2.7347333996310843E-4 36-37 0.0 0.0 0.0 0.04449801917399722 2.7347333996310843E-4 38-39 0.0 9.766904998682445E-6 0.0 0.07735388758956496 3.1254095995783825E-4 40-41 0.0 1.953380999736489E-5 0.0 0.1142141870545925 3.1254095995783825E-4 42-43 0.0 1.953380999736489E-5 0.0 0.14996105934977025 3.3207476995520313E-4 44-45 0.0 1.953380999736489E-5 0.0 0.19677383500845522 3.51608579952568E-4 46-47 0.0 1.953380999736489E-5 0.0 0.2551994607105736 3.51608579952568E-4 48-49 0.0 1.953380999736489E-5 0.0 0.3172486081672031 3.711423899499329E-4 50-51 0.0 1.953380999736489E-5 0.0 0.3854313719630053 3.711423899499329E-4 52-53 0.0 1.953380999736489E-5 0.0 0.4537997069537824 4.1021000994466265E-4 54-55 0.0 1.953380999736489E-5 0.0 0.5345036429578955 4.883452499341223E-4 56-57 0.0 1.953380999736489E-5 0.0 0.6299458386050203 5.176459649301696E-4 58-59 0.0 1.953380999736489E-5 0.0 0.7400676924651648 5.27412869928852E-4 60-61 0.0 1.953380999736489E-5 0.0 0.8564110648094702 5.27412869928852E-4 62-63 0.0 2.9300714996047334E-5 0.0 0.9764365603382786 5.469466799262169E-4 64-65 0.0 3.906761999472978E-5 0.0 1.1014334105114165 5.469466799262169E-4 66-67 0.0 3.906761999472978E-5 0.0 1.2507107865112792 5.469466799262169E-4 68-69 0.0 3.906761999472978E-5 0.0 1.4290251710722246 5.469466799262169E-4 70-71 0.0 3.906761999472978E-5 0.0 1.618131985656714 5.860142999209466E-4 72-73 0.0 3.906761999472978E-5 0.0 1.8050607804264973 6.641495399104063E-4 74-75 0.0 3.906761999472978E-5 0.0 2.000281677540162 6.641495399104063E-4 76-77 0.0 3.906761999472978E-5 0.0 2.2183864330657395 6.739164449090886E-4 78-79 0.0 3.906761999472978E-5 0.0 2.4699037705918103 6.836833499077711E-4 80-81 0.0 3.906761999472978E-5 0.0 2.7406716778702833 7.227509699025008E-4 82-83 0.0 3.906761999472978E-5 0.0 3.0200539953575944 7.813523998945955E-4 84-85 0.0 3.906761999472978E-5 0.0 3.308480466873686 7.813523998945955E-4 86-87 0.0 3.906761999472978E-5 0.0 3.6097894860830393 7.813523998945955E-4 88 0.0 3.906761999472978E-5 0.0 3.854255118200061 7.813523998945955E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10220 0.0 45.78078 1 TACCTGG 5295 0.0 39.42973 2 GTACCTG 5615 0.0 37.68546 1 GTATCAA 16600 0.0 35.210655 1 ACCTGGG 6505 0.0 31.49738 3 TCAACGC 20770 0.0 27.67102 4 ATCAACG 20960 0.0 27.330505 3 CAACGCA 21020 0.0 27.27458 5 AACGCAG 21770 0.0 26.485268 6 TATCAAC 22080 0.0 26.130775 2 TAACGCA 495 0.0 24.683308 4 GTACATG 18835 0.0 23.542746 1 ACGCAGA 24735 0.0 23.215471 7 TACATGG 18950 0.0 22.853735 2 CGCAGAG 25145 0.0 22.68743 8 CATGGGG 10440 0.0 22.191242 4 ACATGGG 19040 0.0 21.744238 3 TATAACG 500 0.0 20.68995 2 CCTGGGG 6205 0.0 20.599768 4 GAGTACT 16370 0.0 20.40982 12-13 >>END_MODULE