FastQCFastQC Report
Mon 6 Jun 2016
ERR1211484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7357616
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260880.3545713720313754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231600.31477587305453286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140410.19083627087904562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132350.049.7792471
GTATCAA222550.036.861681
TCAACGC268150.029.738064
TACCTGG61750.029.699922
ATCAACG269450.029.542673
CAACGCA272000.029.2805945
TATCAAC281150.028.4842132
AACGCAG279400.028.4803316
GTACCTG68550.027.891581
GTACATG250900.025.995831
ACGCAGA312600.025.321347
TACATGG251950.024.9355952
CGCAGAG318750.024.7306338
ACATGGG254650.023.8045793
ACCTGGG80100.022.6448423
GCAGAGT349950.021.6818039
CATGGGG141250.021.3910034
GAGTACT217450.020.39659512-13
CAGAGTA343600.019.77122910-11
AGAGTAC327800.019.1763410-11
Produced by FastQC (version 0.11.5)