FastQCFastQC Report
Mon 6 Jun 2016
ERR1211484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7357616
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279080.37930764530250016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251840.342284783549454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164330.22334680146395247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132950.050.3389471
GTATCAA233550.037.5968971
TCAACGC287700.029.824324
TACCTGG62600.029.7502572
ATCAACG290950.029.5077273
CAACGCA292000.029.4173135
TATCAAC302300.028.6874962
AACGCAG303900.028.3579836
GTACCTG70650.026.561241
TATAACG6600.025.6524262
GTACATG253950.025.4649071
ACGCAGA342850.025.0266827
CGCAGAG348300.024.486688
TACATGG261900.023.9367122
ACATGGG261550.023.1586763
TATACCG4900.023.0157345
ACCTGGG81100.022.2497923
GCAGAGT391750.021.4467879
GAGTACT239000.020.8024112-13
AGAGTAC360000.020.21242110-11
Produced by FastQC (version 0.11.5)