##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7357616 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.162672800537564 33.0 31.0 34.0 30.0 34.0 2 32.376309799261065 34.0 31.0 34.0 31.0 34.0 3 32.38673708983997 34.0 31.0 34.0 30.0 34.0 4 36.01036803225393 37.0 35.0 37.0 35.0 37.0 5 35.93522140867368 37.0 35.0 37.0 35.0 37.0 6 35.95349580625029 37.0 35.0 37.0 35.0 37.0 7 35.88451476673966 37.0 35.0 37.0 35.0 37.0 8 35.87893116465986 37.0 35.0 37.0 35.0 37.0 9 37.54279184996879 39.0 37.0 39.0 35.0 39.0 10-11 37.50508269254607 39.0 37.0 39.0 35.0 39.0 12-13 37.42493030351135 39.0 37.0 39.0 34.0 39.0 14-15 38.675521255798074 40.0 38.0 41.0 34.0 41.0 16-17 38.60366605161237 40.0 38.0 41.0 34.0 41.0 18-19 38.599934611972145 40.0 38.0 41.0 34.0 41.0 20-21 38.546940952069264 40.0 38.0 41.0 34.0 41.0 22-23 38.439825209687484 40.0 38.0 41.0 34.0 41.0 24-25 38.33240284624802 40.0 38.0 41.0 34.0 41.0 26-27 38.16102661514273 40.0 38.0 41.0 33.0 41.0 28-29 38.19780109481114 40.0 38.0 41.0 34.0 41.0 30-31 38.12189620931562 40.0 37.5 41.0 33.0 41.0 32-33 37.93947122274389 40.0 37.0 41.0 33.0 41.0 34-35 37.82366747598679 40.0 37.0 41.0 33.0 41.0 36-37 37.765141861168075 40.0 37.0 41.0 33.0 41.0 38-39 37.67114823605907 40.0 37.0 41.0 33.0 41.0 40-41 37.515454190596515 40.0 37.0 41.0 32.0 41.0 42-43 37.36989739611309 40.0 36.5 41.0 31.0 41.0 44-45 37.18488094513222 40.0 36.0 41.0 31.0 41.0 46-47 36.929287761144366 39.5 36.0 41.0 30.5 41.0 48-49 36.70668963968764 39.0 36.0 41.0 30.0 41.0 50-51 36.50809834326771 39.0 35.0 41.0 29.5 41.0 52-53 36.31552251435791 39.0 35.0 40.5 29.0 41.0 54-55 36.12350393116466 39.0 35.0 40.0 28.0 41.0 56-57 35.92729934533142 38.5 35.0 40.0 28.0 41.0 58-59 35.67521171259821 38.0 34.0 40.0 27.5 41.0 60-61 35.36826942313924 38.0 34.0 40.0 27.0 41.0 62-63 35.054895091018615 37.5 34.0 40.0 26.0 41.0 64-65 34.74491472509574 37.0 33.5 40.0 26.0 41.0 66-67 34.40766757873746 36.5 33.0 39.0 26.0 41.0 68-69 34.01151500703489 36.0 33.0 39.0 25.5 40.5 70-71 33.625130816829795 35.5 32.0 39.0 25.0 40.0 72-73 33.21845221332562 35.0 32.0 38.0 24.0 40.0 74-75 32.80798495056007 35.0 31.0 37.0 23.5 39.5 76-77 31.25573779604698 33.5 29.5 35.5 21.5 38.5 78-79 32.07914771578185 34.5 31.0 36.0 23.0 39.0 80-81 32.038911109250606 35.0 31.0 36.0 23.0 38.0 82-83 31.75042907920174 34.0 31.0 35.5 22.0 37.0 84-85 31.44662197646629 34.0 31.0 35.0 21.0 37.0 86-87 31.14556026027996 34.0 30.0 35.0 20.0 36.0 88-89 30.863763140125826 34.0 30.0 35.0 20.0 36.0 90-91 30.582150794496478 34.0 30.0 35.0 19.5 36.0 92-93 30.37071043120489 34.0 29.0 35.0 19.0 35.0 94-95 30.150671562636596 34.0 29.0 35.0 18.0 35.0 96-97 29.945109462086634 34.0 29.0 35.0 18.0 35.0 98-99 29.780365474360174 34.0 29.0 35.0 18.0 35.0 100 27.916442635766803 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33096905225357176 1101 2 0.28904424814684404 1101 3 0.3170827873390891 1101 4 0.18489324487865133 1101 5 0.19037642578018676 1101 6 0.19017101353961863 1101 7 0.2016275177809561 1101 8 0.22325446138386695 1101 9 0.2536588617256754 1101 10-11 0.29114543528152836 1101 12-13 0.28678110760646547 1101 14-15 0.36972742865498986 1101 16-17 0.3506565397594201 1101 18-19 0.3715777007578751 1101 20-21 0.30569332168899876 1101 22-23 0.3226229177032991 1101 24-25 0.2673903688848256 1101 26-27 0.2207241115938814 1101 28-29 0.3327223803157153 1101 30-31 0.39765739117829924 1101 32-33 0.42141351691965667 1101 34-35 0.37377908136846116 1101 36-37 0.40391967355832037 1101 38-39 0.3742552322351713 1101 40-41 0.3822007217690242 1101 42-43 0.3910645675926503 1101 44-45 0.37582904473332945 1101 46-47 0.3919640685524186 1101 48-49 0.4075970147294541 1101 50-51 0.4162112204710624 1101 52-53 0.41402122765070004 1101 54-55 0.3849127038495581 1101 56-57 0.3307086138406845 1101 58-59 0.37789223993591037 1101 60-61 0.311208194509085 1101 62-63 0.35597962796149574 1101 64-65 0.3721222090503886 1101 66-67 0.23269418230680117 1101 68-69 0.27206330589948635 1101 70-71 0.25527341877171494 1101 72-73 0.15249127804982976 1101 74-75 0.18212393873893262 1101 76-77 0.2844119094238522 1101 78-79 0.3125133180004198 1101 80-81 0.30948455559456534 1101 82-83 0.3107948627489385 1101 84-85 0.3554239018961347 1101 86-87 0.25588222289966467 1101 88-89 0.21267393235445198 1101 90-91 0.18999442897249352 1101 92-93 0.1475347032117682 1101 94-95 0.20726504581109495 1101 96-97 0.16547020435953996 1101 98-99 0.04952706098482551 1101 100 0.03882722026314056 1102 1 0.34397972344631 1102 2 0.24058257261553706 1102 3 0.3462140068635904 1102 4 0.21333452726520363 1102 5 0.22515837911355874 1102 6 0.23612520968599426 1102 7 0.22112763924040024 1102 8 0.22514391098187048 1102 9 0.2975388897527509 1102 10-11 0.24646100889832212 1102 12-13 0.267248375989638 1102 14-15 0.36281414766107645 1102 16-17 0.3644409748661701 1102 18-19 0.30401949245921145 1102 20-21 0.3569867833900773 1102 22-23 0.31951917758456716 1102 24-25 0.34079617328730905 1102 26-27 0.3235870561683569 1102 28-29 0.35553083336063196 1102 30-31 0.37486420107655505 1102 32-33 0.44482027154710124 1102 34-35 0.4794398052042439 1102 36-37 0.5355821635532863 1102 38-39 0.49706896398598843 1102 40-41 0.46774498059159697 1102 42-43 0.43205517356594214 1102 44-45 0.40566878614592383 1102 46-47 0.4784009381608385 1102 48-49 0.5575274022476933 1102 50-51 0.5082564726586654 1102 52-53 0.521311808020748 1102 54-55 0.4950183238504948 1102 56-57 0.474340833619884 1102 58-59 0.52220306362927 1102 60-61 0.4609339616123762 1102 62-63 0.5655577855420262 1102 64-65 0.5216620635187681 1102 66-67 0.46350014835319087 1102 68-69 0.5037642781960088 1102 70-71 0.48161692432715597 1102 72-73 0.4309856840183244 1102 74-75 0.44402292271905 1102 76-77 0.46398414116127285 1102 78-79 0.43452097963603364 1102 80-81 0.49191456899338704 1102 82-83 0.4711756912012284 1102 84-85 0.3991427680069499 1102 86-87 0.3155383951796047 1102 88-89 0.34620189559334946 1102 90-91 0.3613725010776463 1102 92-93 0.25924032690650023 1102 94-95 0.31607089846734837 1102 96-97 0.3264878427493123 1102 98-99 0.2845701184784417 1102 100 0.3926416092396181 1103 1 0.26185900584600574 1103 2 0.22709120470696575 1103 3 0.3019127439561373 1103 4 0.13906152407544425 1103 5 0.15017793995336604 1103 6 0.15921937260633 1103 7 0.1872444337866881 1103 8 0.20631889121879965 1103 9 0.2086793583187898 1103 10-11 0.21048841904457305 1103 12-13 0.2804033446408738 1103 14-15 0.3613719386640071 1103 16-17 0.3273842541606271 1103 18-19 0.36838444783906965 1103 20-21 0.2866461569288745 1103 22-23 0.29164912768038675 1103 24-25 0.2550331013158811 1103 26-27 0.2900836866323502 1103 28-29 0.3491325853188414 1103 30-31 0.41746277845098945 1103 32-33 0.46258058755314124 1103 34-35 0.45427041549569935 1103 36-37 0.45167789666191993 1103 38-39 0.39100163057913306 1103 40-41 0.3892233886701959 1103 42-43 0.4380509673596862 1103 44-45 0.4335864105597551 1103 46-47 0.486690405630533 1103 48-49 0.4310966870771722 1103 50-51 0.4695585389399568 1103 52-53 0.4443073940327906 1103 54-55 0.4801700640900606 1103 56-57 0.4623323790974112 1103 58-59 0.465851738224508 1103 60-61 0.47748365909686896 1103 62-63 0.5283817628252478 1103 64-65 0.4770064372076632 1103 66-67 0.5095791804710927 1103 68-69 0.5089066853982089 1103 70-71 0.475862674795394 1103 72-73 0.46267452417860255 1103 74-75 0.42894313702492326 1103 76-77 0.41115566731955866 1103 78-79 0.48012311207558156 1103 80-81 0.4077401371183029 1103 82-83 0.36305931921755885 1103 84-85 0.3425089572977633 1103 86-87 0.3834489423652059 1103 88-89 0.47446579990355886 1103 90-91 0.5045951679549212 1103 92-93 0.3894034538331539 1103 94-95 0.4818062335100066 1103 96-97 0.4895203518589284 1103 98-99 0.495299422516144 1103 100 0.636439959237439 1104 1 0.25207765273127336 1104 2 0.2530133133839385 1104 3 0.2821958000120546 1104 4 0.10018632837501684 1104 5 0.1300963261344279 1104 6 0.12792980334091908 1104 7 0.15929175129195983 1104 8 0.17723302337340385 1104 9 0.19637014618392357 1104 10-11 0.20646407104864295 1104 12-13 0.24804092777123543 1104 14-15 0.30273010911066933 1104 16-17 0.26508406818432917 1104 18-19 0.28591725743542185 1104 20-21 0.2313651680682085 1104 22-23 0.27339987008861044 1104 24-25 0.2926190324839908 1104 26-27 0.38736968202606903 1104 28-29 0.45560038037710626 1104 30-31 0.38958340564990834 1104 32-33 0.319960384010443 1104 34-35 0.4155850691062497 1104 36-37 0.3499521762265516 1104 38-39 0.39801488005324615 1104 40-41 0.48849984657152135 1104 42-43 0.5521999669322284 1104 44-45 0.5304103091070402 1104 46-47 0.4932931193351706 1104 48-49 0.4739226017932552 1104 50-51 0.524582776804273 1104 52-53 0.4918866697826161 1104 54-55 0.5276738099904179 1104 56-57 0.541742674822757 1104 58-59 0.6174334782136484 1104 60-61 0.5242970701923824 1104 62-63 0.5362675632373097 1104 64-65 0.6077542721176457 1104 66-67 0.5551274814385607 1104 68-69 0.6043827785821421 1104 70-71 0.5276369609536715 1104 72-73 0.5609227900512721 1104 74-75 0.5629351170795829 1104 76-77 0.5520568613861805 1104 78-79 0.5970527120186517 1104 80-81 0.6540791892760112 1104 82-83 0.5540032115585127 1104 84-85 0.5782161287545016 1104 86-87 0.5856286943184053 1104 88-89 0.6243621074889028 1104 90-91 0.48610551723726303 1104 92-93 0.4518225480879927 1104 94-95 0.5570235236498888 1104 96-97 0.5543906243675387 1104 98-99 0.5680346081514678 1104 100 0.6303392235060166 1105 1 0.2114891439404616 1105 2 0.1409421264745916 1105 3 0.19827437136078885 1105 4 0.11507642436713894 1105 5 0.10144909439409133 1105 6 0.10764066673547035 1105 7 0.18086212737328822 1105 8 0.17461639000325135 1105 9 0.19628233887961954 1105 10-11 0.17498374012779294 1105 12-13 0.2292463376974041 1105 14-15 0.2871894311544523 1105 16-17 0.27303009770265163 1105 18-19 0.24960384968830596 1105 20-21 0.23582247458355 1105 22-23 0.29740821248108773 1105 24-25 0.26144883659745943 1105 26-27 0.27604268555950284 1105 28-29 0.3256253570144665 1105 30-31 0.39076795382407425 1105 32-33 0.3967621771382568 1105 34-35 0.4726959299737956 1105 36-37 0.44642690582500677 1105 38-39 0.36657186192150704 1105 40-41 0.40798149007642337 1105 42-43 0.3280672667996143 1105 44-45 0.3596455885815004 1105 46-47 0.46759510138080174 1105 48-49 0.4856361447836619 1105 50-51 0.5269277572758782 1105 52-53 0.511371356626114 1105 54-55 0.4476692067129946 1105 56-57 0.45354441388727196 1105 58-59 0.47109454165455844 1105 60-61 0.5156858950287457 1105 62-63 0.5412084933107479 1105 64-65 0.5812860495537393 1105 66-67 0.5272159358015998 1105 68-69 0.5463433805290023 1105 70-71 0.6346933211547281 1105 72-73 0.5962364519566421 1105 74-75 0.610522044184755 1105 76-77 0.5972227853000476 1105 78-79 0.6895642979661787 1105 80-81 0.6359499270833382 1105 82-83 0.6646583803988086 1105 84-85 0.6080684557604421 1105 86-87 0.5725558086065234 1105 88-89 0.5504637717638268 1105 90-91 0.6229943103645894 1105 92-93 0.6978167308768626 1105 94-95 0.6761193556488507 1105 96-97 0.5974555208600059 1105 98-99 0.6203826441107303 1105 100 0.7428041613295768 1106 1 0.20085624049553985 1106 2 0.15618964651467593 1106 3 0.2035820069570562 1106 4 0.12530966838855306 1106 5 0.09415660925112945 1106 6 0.1249982789258155 1106 7 0.14838162717011727 1106 8 0.11085168728853745 1106 9 0.07828362752325546 1106 10-11 0.08226407182381479 1106 12-13 0.13484160536764733 1106 14-15 0.21732298623321356 1106 16-17 0.24693637459686357 1106 18-19 0.24478655898931834 1106 20-21 0.2631860921641831 1106 22-23 0.28956905305345515 1106 24-25 0.26898886575680336 1106 26-27 0.21788338608174485 1106 28-29 0.3593306807365195 1106 30-31 0.31996411790373713 1106 32-33 0.38076565867415013 1106 34-35 0.3188331055615805 1106 36-37 0.3313246865647841 1106 38-39 0.33601791611977205 1106 40-41 0.31686861289402657 1106 42-43 0.31903466151845095 1106 44-45 0.332413773576711 1106 46-47 0.41120899740656824 1106 48-49 0.41908235183449705 1106 50-51 0.46464229480289987 1106 52-53 0.5158727187622887 1106 54-55 0.416590507805239 1106 56-57 0.48511648823568976 1106 58-59 0.583560061591406 1106 60-61 0.5458457436082043 1106 62-63 0.5471786563229983 1106 64-65 0.6468594803346548 1106 66-67 0.555149131724491 1106 68-69 0.5735707502404352 1106 70-71 0.5796013262181603 1106 72-73 0.5387605884103834 1106 74-75 0.5744343485095698 1106 76-77 0.5861968717565205 1106 78-79 0.6858068858304165 1106 80-81 0.6743098750407981 1106 82-83 0.6023865665401082 1106 84-85 0.6372420300059467 1106 86-87 0.5549891112611256 1106 88-89 0.565192875288588 1106 90-91 0.5517969207838149 1106 92-93 0.5748911154822132 1106 94-95 0.6352706093094405 1106 96-97 0.7042151828652159 1106 98-99 0.7616353105049107 1106 100 0.832701887415336 1107 1 0.16571430627490003 1107 2 0.14655978976526285 1107 3 0.1719580355216621 1107 4 0.11529391486977403 1107 5 0.09517154463589605 1107 6 0.15524728404317045 1107 7 0.12147274974784494 1107 8 0.12794542076257187 1107 9 0.14246964004073703 1107 10-11 0.1332622414574942 1107 12-13 0.11501386645973355 1107 14-15 0.18404963465869173 1107 16-17 0.13415512413810404 1107 18-19 0.140503796297601 1107 20-21 0.09779975700474353 1107 22-23 0.1994635014759183 1107 24-25 0.2217931756383038 1107 26-27 0.22504970861978535 1107 28-29 0.23736086082082863 1107 30-31 0.09913664053645022 1107 32-33 0.152568127974412 1107 34-35 0.16875819544199544 1107 36-37 0.231416782083258 1107 38-39 0.23937437871759215 1107 40-41 0.1890750447268843 1107 42-43 0.20694459787192443 1107 44-45 0.2186861799878912 1107 46-47 0.2927550345700709 1107 48-49 0.3166608316858657 1107 50-51 0.2756865080142674 1107 52-53 0.23730746831535754 1107 54-55 0.2983173071396479 1107 56-57 0.3486463806671125 1107 58-59 0.402546415615582 1107 60-61 0.3318055019525943 1107 62-63 0.2664006298943633 1107 64-65 0.3375321269277478 1107 66-67 0.3739976590735665 1107 68-69 0.41930101977842327 1107 70-71 0.382842457353874 1107 72-73 0.400083499285266 1107 74-75 0.4053324352784884 1107 76-77 0.37560377545270995 1107 78-79 0.4413512957460952 1107 80-81 0.4184411042550664 1107 82-83 0.4253226277613784 1107 84-85 0.35956823245781067 1107 86-87 0.27886249990503487 1107 88-89 0.3045155903178731 1107 90-91 0.28219661679751695 1107 92-93 0.3851243033122529 1107 94-95 0.5094477057298583 1107 96-97 0.5177591827805124 1107 98-99 0.5877757336499805 1107 100 0.7540693424857281 1108 1 0.22602470614651793 1108 2 0.17546707430191333 1108 3 0.22979411403184002 1108 4 0.12934066660987042 1108 5 0.1603249245141143 1108 6 0.14585765664153172 1108 7 0.15840376091589548 1108 8 0.15521305609001956 1108 9 0.14266837781515562 1108 10-11 0.15246652479463307 1108 12-13 0.20256531299143887 1108 14-15 0.21671817769615132 1108 16-17 0.2532289227804796 1108 18-19 0.27130678214899717 1108 20-21 0.2428349380369852 1108 22-23 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70-71 -0.34418393503415246 2316 72-73 -0.4091140024166293 2316 74-75 -0.3829424919099864 2316 76-77 -0.4225559717266272 2316 78-79 -0.40719093099750836 2316 80-81 -0.28578356252533865 2316 82-83 -0.1996256988009577 2316 84-85 -0.23966595520015943 2316 86-87 -0.2177241694427643 2316 88-89 -0.1812689750848797 2316 90-91 -0.23341302744486825 2316 92-93 -0.10821362455723715 2316 94-95 -0.10188183986515753 2316 96-97 -0.05228325831510716 2316 98-99 -0.19627266462359927 2316 100 -0.35073495496477136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 10.0 11 34.0 12 76.0 13 234.0 14 668.0 15 1815.0 16 4125.0 17 8703.0 18 15400.0 19 23886.0 20 34025.0 21 46568.0 22 60969.0 23 70191.0 24 73978.0 25 80563.0 26 92268.0 27 109694.0 28 132995.0 29 159275.0 30 191356.0 31 230869.0 32 282570.0 33 355312.0 34 464752.0 35 634554.0 36 936705.0 37 1490778.0 38 1583246.0 39 271961.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76927025872456 20.330210791614004 14.277401910821014 23.623117038840423 2 14.855212418229534 21.470350454171545 40.255958417298125 23.418478710300793 3 17.009024603696364 27.180626493618192 31.094685964205766 24.71566293847968 4 11.792511555436192 18.03952823592988 38.773924999211694 31.394035209422235 5 12.315410869115809 38.322231946252316 35.85228601768892 13.51007116694296 6 29.95152614822139 38.59643972368613 18.19411966578662 13.257914462305855 7 26.172254708590394 33.1933196839846 23.2040650123627 17.430360595062314 8 25.249597047064114 36.38854951757777 20.64744570855633 17.714407726801785 9 26.145527612620068 15.400604054520937 20.72343017722865 37.730438155630345 10-11 24.028523641353395 26.706843629784434 29.480581753654988 19.784050975207187 12-13 24.78953780681134 24.650253560392386 29.24846852567462 21.311740107121654 14-15 22.19704725008753 26.022566276902737 26.87664047702408 24.903745995985656 16-17 21.617342879710524 28.8283927997553 27.608633057542004 21.945631262992173 18-19 21.426956467314042 27.885439434550175 29.60008850783764 21.087515590298146 20-21 22.28927211592561 27.092868127464236 29.603635047208083 21.01422470940207 22-23 22.031565659311386 27.084683408321393 29.301454438502905 21.582296493864316 24-25 21.754988300558225 27.224518104777417 29.428492870516752 21.592000724147603 26-27 21.770842068623672 27.308240301500202 29.395243589389864 21.525674040486262 28-29 21.686103030795206 27.240779293943678 29.580883329827866 21.49223434543325 30-31 22.03316516725737 27.169605036813753 29.48240562685045 21.31482416907843 32-33 21.779115630161048 27.366825522667863 29.3856713182665 21.468387528904582 34-35 22.033601932226635 27.400444319700867 29.354037444366593 21.2119163037059 36-37 22.03019483954876 27.306731463902455 29.211137917412472 21.451935779136313 38-39 21.918812226898552 27.457467037544482 29.22098737485473 21.402733360702232 40-41 21.986422572357924 27.312109167454896 29.292655871905808 21.408812388281376 42-43 21.858792252746508 27.33451536442194 29.151403937728 21.655288445103547 44-45 21.906713658926446 27.547429470834174 28.97313394696924 21.572722923270142 46-47 21.906661013160576 27.50209610828961 28.760162055998663 21.831080822551154 48-49 21.858292649741152 27.550778655206166 28.797316126632953 21.79361256841973 50-51 21.953223248427207 27.734019683055077 28.562415760362803 21.750341308154912 52-53 21.962833276292745 27.79828943473274 28.406410528596364 21.832466760378146 54-55 21.877786933290295 27.80147299926095 28.508533032050902 21.812207035397854 56-57 21.970116459175678 27.8437926949472 28.30223415030361 21.88385669557351 58-59 21.935713747867105 27.715241878420212 28.503376340525843 21.84566803318684 60-61 22.049760189075887 27.89317886553924 28.40298266450964 21.654078280875233 62-63 21.85771502919132 27.87774193445341 28.52394806888851 21.740594967466762 64-65 21.831496091032147 27.67044843681382 28.48112483456936 22.01693063758467 66-67 21.80075772944865 27.75533949833811 28.352993588626106 22.090909183587126 68-69 21.770616155489662 27.791577779221665 28.417797588641186 22.020008476647487 70-71 21.81895830051396 27.609199654922552 28.414683923438695 22.15715812112479 72-73 21.750879004111365 27.84360166221572 28.189365367017132 22.21615396665578 74-75 21.7192034131669 27.907804262109252 28.134114135583406 22.238878189140443 76-77 21.649470243703867 27.786005707864575 28.260786684956663 22.303737363474898 78-79 21.66638802123686 27.70468205876836 28.201105882544837 22.427824037449945 80-81 21.658776655716387 27.903101087602295 28.01412169016091 22.424000566520412 82-83 21.698083391406307 27.920565281508747 28.051124968573305 22.33022635851164 84-85 21.651490384125225 27.882638409803807 27.986243130911152 22.47962807515981 86-87 21.506939196883383 27.849576610328224 28.168020347663695 22.475463845124693 88-89 21.511315829845305 27.74178713011235 28.09401657871013 22.652880461332213 90-91 21.65336296262662 27.786516555306445 28.025002367184946 22.535118114881982 92-93 21.540397218272904 27.727760398063957 28.02531992186539 22.70652246179775 94-95 21.37922323227461 27.632712481511906 28.054692392682046 22.933371893531433 96-97 21.370787312669666 27.59439222136222 28.147166974164033 22.887653491804084 98-99 21.307259289065186 27.502871715539072 28.040164595179867 23.14970440021587 100 20.53051676507364 27.92256748266017 28.193794991045635 23.353120761220556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2482.0 1 1785.5 2 1906.5 3 2819.0 4 2245.5 5 1821.0 6 2746.0 7 5490.5 8 8896.0 9 10635.5 10 9308.0 11 7375.5 12 7402.5 13 7850.5 14 8367.5 15 9009.0 16 9563.0 17 10185.0 18 11007.0 19 11883.5 20 13330.0 21 15452.0 22 18298.0 23 22670.5 24 28426.5 25 35179.5 26 43793.0 27 54380.0 28 65922.5 29 80059.0 30 95707.5 31 110624.5 32 128151.5 33 147489.0 34 166987.0 35 184538.5 36 204918.5 37 223835.5 38 235714.5 39 246897.0 40 251527.0 41 252236.0 42 254823.0 43 258469.5 44 262689.0 45 268774.5 46 273144.0 47 273122.0 48 273840.0 49 274929.0 50 272277.5 51 260736.0 52 244928.5 53 232270.0 54 218554.5 55 201082.0 56 180940.5 57 159917.5 58 140068.0 59 119605.5 60 99014.0 61 79926.5 62 63654.5 63 49126.0 64 36053.0 65 26681.0 66 19593.5 67 14415.5 68 10786.0 69 7977.5 70 5940.0 71 4431.5 72 3341.5 73 2527.0 74 1867.5 75 1354.0 76 980.5 77 655.5 78 439.5 79 300.5 80 200.5 81 125.0 82 89.5 83 72.5 84 58.0 85 42.0 86 26.5 87 18.5 88 16.0 89 17.0 90 10.0 91 4.0 92 2.0 93 1.0 94 2.5 95 2.0 96 1.0 97 5.0 98 5.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034657965297455046 2 0.07241476043327078 3 8.562556132312423E-4 4 5.43654357607138E-4 5 0.0017261025854026632 6 5.572457165473164E-4 7 0.0 8 7.747074595901717E-4 9 2.71827178803569E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.222772158807962E-4 18-19 0.0010261475999834729 20-21 0.0032959045429932737 22-23 0.0 24-25 0.0 26-27 1.2232223046160603E-4 28-29 7.950944980004394E-4 30-31 0.0014610710860691832 32-33 0.0029765076078990807 34-35 0.004090999040993713 36-37 0.0013659315734879341 38-39 0.0015833933165307892 40-41 0.005504500370772272 42-43 0.006870431944260206 44-45 0.005110350961507097 46-47 0.007196624558824489 48-49 0.008657695644893673 50-51 0.008535373414432066 52-53 0.0038327632211303227 54-55 0.0024260575708218535 56-57 0.0025551754807535483 58-59 0.0032891088635231846 60-61 0.0077470745959017165 62-63 0.014440818873939602 64-65 0.00411818175887407 66-67 0.010444959345527138 68-69 0.012694329250126672 70-71 0.005803510267456197 72-73 0.007726687557491448 74-75 0.0030716471204803295 76-77 0.0030376687231298835 78-79 0.008100449928346356 80-81 0.005749144831695484 82-83 0.016452339997086014 84-85 0.017546444391770378 86-87 0.022908235493670777 88-89 0.021956840367858284 90-91 0.024906165257877005 92-93 0.035568586346447 94-95 0.03917029646559429 96-97 0.023465481210218093 98-99 0.018837623491087332 100 0.019435643284455184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7357616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.93856020843778 #Duplication Level Percentage of deduplicated Percentage of total 1 73.96558304134896 45.07356135517448 2 16.38799515379266 19.97321658749961 3 4.928557217637117 9.0101754224313 4 1.9423057805733914 4.7344527101067335 5 0.9302357567044889 2.834361383398909 6 0.5011628122585992 1.8324084125430387 7 0.3124537081065687 1.3328335372661246 8 0.20058544185475452 0.9778710420321644 9 0.1466219626931155 0.8041438171308363 >10 0.6256202690443633 6.548861628829568 >50 0.03359539463743591 1.4168885806789644 >100 0.02279300429816811 2.644309776602707 >500 0.0016910545970519766 0.7199092301612003 >1k 7.071637087706161E-4 0.7943638003873201 >5k 0.0 0.0 >10k+ 9.223874461662406E-5 1.3026427157569473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27908 0.37930764530250016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25184 0.342284783549454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16433 0.22334680146395247 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.3591358940178448E-5 0.0 0.0 0.0 3 0.0 1.3591358940178448E-5 0.0 0.0 0.0 4 0.0 1.3591358940178448E-5 0.0 0.0 0.0 5 0.0 2.7182717880356897E-5 0.0 0.0 0.0 6 0.0 2.7182717880356897E-5 0.0 0.0 0.0 7 0.0 2.7182717880356897E-5 0.0 0.0 1.3591358940178448E-5 8 0.0 2.7182717880356897E-5 0.0 0.0 1.3591358940178448E-5 9 0.0 2.7182717880356897E-5 0.0 0.0 1.3591358940178448E-5 10-11 0.0 2.7182717880356897E-5 0.0 1.3591358940178448E-5 1.3591358940178448E-5 12-13 0.0 2.7182717880356897E-5 0.0 1.3591358940178448E-5 1.3591358940178448E-5 14-15 0.0 2.7182717880356897E-5 0.0 2.0387038410267673E-5 2.7182717880356897E-5 16-17 0.0 2.7182717880356897E-5 0.0 4.0774076820535345E-5 4.0774076820535345E-5 18-19 0.0 2.7182717880356897E-5 0.0 4.0774076820535345E-5 6.795679470089225E-5 20-21 0.0 2.7182717880356897E-5 0.0 5.4365435760713794E-5 7.475247417098147E-5 22-23 0.0 2.7182717880356897E-5 0.0 6.795679470089225E-5 1.0193519205133837E-4 24-25 0.0 3.397839735044612E-5 0.0 1.7668766622231985E-4 1.2232223046160603E-4 26-27 0.0 4.0774076820535345E-5 0.0 3.3978397350446126E-4 1.2232223046160603E-4 28-29 0.0 4.0774076820535345E-5 0.0 9.853735231629376E-4 1.2911790993169525E-4 30-31 0.0 4.0774076820535345E-5 0.0 0.00394149409265175 1.359135894017845E-4 32-33 0.0 4.0774076820535345E-5 0.0 0.010676012447510172 1.4950494834196294E-4 34-35 0.0 4.756975629062457E-5 0.0 0.02148793848442213 1.4950494834196294E-4 36-37 6.795679470089224E-6 6.116111523080301E-5 0.0 0.03982947737419294 1.4950494834196294E-4 38-39 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.06869752376313197 1.8348334569240907E-4 40-41 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.10219342787120175 1.902790251624983E-4 42-43 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.13562817086404072 1.902790251624983E-4 44-45 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.1779720496421667 1.902790251624983E-4 46-47 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.23110746741879434 1.902790251624983E-4 48-49 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.2901347392959893 1.902790251624983E-4 50-51 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.35226084101154503 1.902790251624983E-4 52-53 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.4141490939456476 2.1746174304285517E-4 54-55 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.48631920991799515 2.4464446092321206E-4 56-57 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.5733242941735475 2.4464446092321206E-4 58-59 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.6721062909507645 2.4464446092321206E-4 60-61 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.7774189356987372 2.4464446092321206E-4 62-63 1.3591358940178448E-5 6.795679470089225E-5 0.0 0.8865303652704898 2.5823581986339055E-4 64-65 1.3591358940178448E-5 6.795679470089225E-5 0.0 1.0009288334699717 2.5823581986339055E-4 66-67 1.3591358940178448E-5 7.475247417098147E-5 0.0 1.135972575899585 2.650314993334798E-4 68-69 1.3591358940178448E-5 8.154815364107069E-5 0.0 1.294169198283792 2.71827178803569E-4 70-71 1.3591358940178448E-5 9.513951258124915E-5 0.0 1.467832787141922 3.397839735044612E-4 72-73 1.3591358940178448E-5 1.0873087152142759E-4 0.0 1.6458727935787896 3.533753324446397E-4 74-75 1.3591358940178448E-5 1.0873087152142759E-4 0.0 1.8271611348023598 3.533753324446397E-4 76-77 2.0387038410267673E-5 1.0873087152142759E-4 0.0 2.029529673742147 3.533753324446397E-4 78-79 2.7182717880356897E-5 1.0873087152142759E-4 0.0 2.263015628975473 3.533753324446397E-4 80-81 2.7182717880356897E-5 1.0873087152142759E-4 0.0 2.5189001437422123 3.601710119147289E-4 82-83 2.7182717880356897E-5 1.0873087152142759E-4 0.0 2.7874925791180187 3.805580503249966E-4 84-85 2.7182717880356897E-5 1.0873087152142759E-4 0.0 3.0575256985414843 3.805580503249966E-4 86-87 2.7182717880356897E-5 1.0873087152142759E-4 0.0 3.340484200317059 3.805580503249966E-4 88 2.7182717880356897E-5 1.0873087152142759E-4 0.0 3.5693083194339037 3.805580503249966E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13295 0.0 50.338947 1 GTATCAA 23355 0.0 37.596897 1 TCAACGC 28770 0.0 29.82432 4 TACCTGG 6260 0.0 29.750257 2 ATCAACG 29095 0.0 29.507727 3 CAACGCA 29200 0.0 29.417313 5 TATCAAC 30230 0.0 28.687496 2 AACGCAG 30390 0.0 28.357983 6 GTACCTG 7065 0.0 26.56124 1 TATAACG 660 0.0 25.652426 2 GTACATG 25395 0.0 25.464907 1 ACGCAGA 34285 0.0 25.026682 7 CGCAGAG 34830 0.0 24.48668 8 TACATGG 26190 0.0 23.936712 2 ACATGGG 26155 0.0 23.158676 3 TATACCG 490 0.0 23.015734 5 ACCTGGG 8110 0.0 22.249792 3 GCAGAGT 39175 0.0 21.446787 9 GAGTACT 23900 0.0 20.80241 12-13 AGAGTAC 36000 0.0 20.212421 10-11 >>END_MODULE