FastQCFastQC Report
Mon 6 Jun 2016
ERR1211483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7370740
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227000.30797450459519665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199610.270814056661882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127170.17253355836727383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119400.048.3779681
GTATCAA202550.035.5603941
TCAACGC235450.029.4963954
ATCAACG237700.029.2181823
CAACGCA239200.029.1488695
AACGCAG248900.028.0651446
TATCAAC255400.027.5029562
TACCTGG63050.025.2792552
GTACATG228750.025.1488131
ACGCAGA281200.024.6247737
GTACCTG70150.024.159181
CGCAGAG284900.024.1082468
TACATGG231350.024.0010412
ACATGGG229300.023.505383
GCAGAGT321500.021.073629
CATGGGG125150.021.063244
ACCTGGG73800.020.5662023
GAGTACT198650.020.43587712-13
CAGAGTA317650.018.9325310-11
AGAGTAC295950.018.66966410-11
Produced by FastQC (version 0.11.5)