FastQCFastQC Report
Mon 6 Jun 2016
ERR1211483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7370740
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247680.3360313889785829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220430.2990608812683665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155140.21048090151056745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126550.047.930621
GTATCAA208250.035.1777421
TCAACGC256500.027.9745464
ATCAACG258300.027.7614123
CAACGCA262300.027.319775
TACCTGG61150.026.4500712
AACGCAG272350.026.3456156
TATCAAC274900.026.254182
GTACATG237400.024.5995061
GTACCTG70550.024.0600031
TACATGG242300.023.324692
ACATGGG234950.023.1003173
ACGCAGA309050.023.0041837
CGCAGAG310300.022.790378
CATGGGG123100.020.7277984
ACCTGGG76750.020.6942523
GCAGAGT350000.019.6951269
GAGTACT221300.019.45026412-13
AGAGTAC325950.019.137810-11
AGTACTT237150.018.06113212-13
Produced by FastQC (version 0.11.5)