##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7370740 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.076824579350244 33.0 31.0 34.0 30.0 34.0 2 32.301236375180785 34.0 31.0 34.0 30.0 34.0 3 32.31691729731343 34.0 31.0 34.0 30.0 34.0 4 35.964368706534216 37.0 35.0 37.0 35.0 37.0 5 35.882841885618 37.0 35.0 37.0 35.0 37.0 6 35.90085568070506 37.0 35.0 37.0 35.0 37.0 7 35.828113866450316 37.0 35.0 37.0 35.0 37.0 8 35.81924555743385 37.0 35.0 37.0 35.0 37.0 9 37.47468937989944 39.0 37.0 39.0 35.0 39.0 10-11 37.42739378949739 39.0 37.0 39.0 34.0 39.0 12-13 37.343368644125285 39.0 37.0 39.0 34.0 39.0 14-15 38.573885254397794 40.0 38.0 41.0 34.0 41.0 16-17 38.50063989504446 40.0 38.0 41.0 34.0 41.0 18-19 38.49162445019089 40.0 38.0 41.0 34.0 41.0 20-21 38.4343769417996 40.0 38.0 41.0 34.0 41.0 22-23 38.330077712685565 40.0 38.0 41.0 34.0 41.0 24-25 38.218845394085264 40.0 38.0 41.0 33.5 41.0 26-27 38.04240354428457 40.0 37.0 41.0 33.0 41.0 28-29 38.07817966717046 40.0 38.0 41.0 33.0 41.0 30-31 38.00529505856942 40.0 37.0 41.0 33.0 41.0 32-33 37.83216257797724 40.0 37.0 41.0 33.0 41.0 34-35 37.720963973766544 40.0 37.0 41.0 33.0 41.0 36-37 37.66972678455623 40.0 37.0 41.0 33.0 41.0 38-39 37.57966364299921 40.0 37.0 41.0 32.0 41.0 40-41 37.420344632967655 40.0 36.5 41.0 31.5 41.0 42-43 37.27303377951196 40.0 36.0 41.0 31.0 41.0 44-45 37.09262869128473 39.5 36.0 41.0 31.0 41.0 46-47 36.84130012725995 39.0 36.0 41.0 30.0 41.0 48-49 36.62652094633646 39.0 35.0 41.0 30.0 41.0 50-51 36.43455378971447 39.0 35.0 40.5 29.5 41.0 52-53 36.23937094511541 39.0 35.0 40.0 28.5 41.0 54-55 36.04929783169668 39.0 35.0 40.0 28.0 41.0 56-57 35.85727708479746 38.0 35.0 40.0 28.0 41.0 58-59 35.60493864930794 38.0 34.0 40.0 27.5 41.0 60-61 35.296403888890396 38.0 34.0 40.0 26.5 41.0 62-63 34.979373644980015 37.5 34.0 40.0 26.0 41.0 64-65 34.66933387963759 37.0 33.0 40.0 26.0 41.0 66-67 34.334692513913126 36.5 33.0 39.0 26.0 41.0 68-69 33.937588830972196 36.0 32.5 39.0 25.0 40.0 70-71 33.55109778665371 35.5 32.0 39.0 24.5 40.0 72-73 33.14321852351324 35.0 31.5 38.0 24.0 40.0 74-75 32.73171981375005 35.0 31.0 37.0 23.0 39.0 76-77 31.18555423200384 33.5 29.5 35.5 21.5 38.5 78-79 32.02984327218162 34.0 31.0 36.0 22.5 39.0 80-81 31.993227749181223 34.0 31.0 36.0 23.0 38.0 82-83 31.699448630666666 34.0 31.0 35.5 22.0 37.0 84-85 31.38870052396367 34.0 30.5 35.0 21.0 37.0 86-87 31.086324032593744 34.0 30.0 35.0 20.0 36.0 88-89 30.791586055131503 34.0 30.0 35.0 20.0 36.0 90-91 30.49708672399243 34.0 29.0 35.0 19.5 36.0 92-93 30.278025747753958 34.0 29.0 35.0 19.0 35.0 94-95 30.04911799900688 34.0 29.0 35.0 18.0 35.0 96-97 29.8388912782163 34.0 29.0 35.0 18.0 35.0 98-99 29.66766294836068 33.5 29.0 35.0 18.0 35.0 100 27.782489275160973 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34680783482491506 1101 2 0.2539879458413026 1101 3 0.33391190512353575 1101 4 0.19540805868248157 1101 5 0.2481866425467132 1101 6 0.20925752071902082 1101 7 0.2458421952598968 1101 8 0.20922879337738465 1101 9 0.2464166773229195 1101 10-11 0.24672019022917624 1101 12-13 0.2821184538483976 1101 14-15 0.3554540481487436 1101 16-17 0.3061672860114868 1101 18-19 0.33068306959201266 1101 20-21 0.30290463194113926 1101 22-23 0.35150034342570535 1101 24-25 0.29558891892034467 1101 26-27 0.2847915810028425 1101 28-29 0.3800728099044761 1101 30-31 0.36918646093278795 1101 32-33 0.4405470507721958 1101 34-35 0.38322717023199004 1101 36-37 0.3550068932528987 1101 38-39 0.39152335010342654 1101 40-41 0.39704483442865524 1101 42-43 0.39718380923461893 1101 44-45 0.39375961640371315 1101 46-47 0.4163938992534213 1101 48-49 0.47350437418364777 1101 50-51 0.47860146310067364 1101 52-53 0.39488151313715747 1101 54-55 0.4149162423839172 1101 56-57 0.3583000905717597 1101 58-59 0.4149073049354257 1101 60-61 0.4104093130771105 1101 62-63 0.3219904118293755 1101 64-65 0.3450890784361107 1101 66-67 0.2867271744456943 1101 68-69 0.2118536779392528 1101 70-71 0.30173722147435456 1101 72-73 0.17704221096610695 1101 74-75 0.13627595524844338 1101 76-77 0.25019842715942175 1101 78-79 0.383259916917261 1101 80-81 0.29392147360222154 1101 82-83 0.2619645302440219 1101 84-85 0.2641578452415452 1101 86-87 0.22073302832361819 1101 88-89 0.19407858149767776 1101 90-91 0.1561927538392318 1101 92-93 0.02869130422529409 1101 94-95 0.07625075989596652 1101 96-97 0.11815960165258232 1101 98-99 -0.026757689728434286 1101 100 0.016422093460437992 1102 1 0.3270214604582691 1102 2 0.2524299520830766 1102 3 0.3138495058750834 1102 4 0.20916553258459913 1102 5 0.20659185286608306 1102 6 0.15624709315346053 1102 7 0.18451986906836737 1102 8 0.169136071449131 1102 9 0.20388029707094546 1102 10-11 0.2301293862393763 1102 12-13 0.28601259088024733 1102 14-15 0.38072084755057034 1102 16-17 0.33393162836664203 1102 18-19 0.3230105068966509 1102 20-21 0.3502876014585965 1102 22-23 0.33608805290480603 1102 24-25 0.3316432594008205 1102 26-27 0.2937498486783241 1102 28-29 0.3260879294616501 1102 30-31 0.355860195023773 1102 32-33 0.4870575745982748 1102 34-35 0.45613230376480374 1102 36-37 0.47055593749440305 1102 38-39 0.4672374789783902 1102 40-41 0.44894801181460764 1102 42-43 0.39604556094928256 1102 44-45 0.43273390295118475 1102 46-47 0.49685101562351974 1102 48-49 0.47001684078365 1102 50-51 0.48593586604326333 1102 52-53 0.45884742156837177 1102 54-55 0.4369524983969697 1102 56-57 0.4735165676610791 1102 58-59 0.5251215705594916 1102 60-61 0.4685335113939857 1102 62-63 0.529600717941527 1102 64-65 0.5290289028549537 1102 66-67 0.5120458725646913 1102 68-69 0.4762029506669947 1102 70-71 0.4023522735012577 1102 72-73 0.43845388935145735 1102 74-75 0.39830134066335887 1102 76-77 0.4549363628260892 1102 78-79 0.4653123033461739 1102 80-81 0.38722202803075945 1102 82-83 0.4067518752273003 1102 84-85 0.3862703223919972 1102 86-87 0.34990050948657014 1102 88-89 0.31968722745481415 1102 90-91 0.2840152992976748 1102 92-93 0.24374725673052566 1102 94-95 0.330177682200663 1102 96-97 0.3629858991759747 1102 98-99 0.31175374980510995 1102 100 0.33728907843184075 1103 1 0.3035022285920732 1103 2 0.29109954582357034 1103 3 0.3292793750535665 1103 4 0.17664911196091992 1103 5 0.19146317006278224 1103 6 0.228368077341365 1103 7 0.23132931018401592 1103 8 0.23271104008708932 1103 9 0.2703667290117622 1103 10-11 0.2369164251492748 1103 12-13 0.3108596508733257 1103 14-15 0.3486334269962086 1103 16-17 0.3932806379040841 1103 18-19 0.37997450421790546 1103 20-21 0.3676106613876087 1103 22-23 0.37552000734641666 1103 24-25 0.36478290840049965 1103 26-27 0.3586591880909751 1103 28-29 0.4074254607694172 1103 30-31 0.5063377858561537 1103 32-33 0.5055118238983098 1103 34-35 0.4811546303633065 1103 36-37 0.530278783746823 1103 38-39 0.5144092734889512 1103 40-41 0.6053117399141428 1103 42-43 0.6635308845326193 1103 44-45 0.5890193812823696 1103 46-47 0.7098026885126814 1103 48-49 0.6465225593123591 1103 50-51 0.6361199884294066 1103 52-53 0.6384108098694341 1103 54-55 0.6928765366090204 1103 56-57 0.6878540691750885 1103 58-59 0.7415831943368758 1103 60-61 0.7474129085361554 1103 62-63 0.8103618599434839 1103 64-65 0.808025229240549 1103 66-67 0.7417215863009474 1103 68-69 0.7166460960307006 1103 70-71 0.7592017520282752 1103 72-73 0.741140028055888 1103 74-75 0.6639497896740494 1103 76-77 0.6621055627351176 1103 78-79 0.694958605110763 1103 80-81 0.7211326539645277 1103 82-83 0.6836271859843563 1103 84-85 0.6387408905827812 1103 86-87 0.7131166954882104 1103 88-89 0.6508642692587401 1103 90-91 0.6571218502847387 1103 92-93 0.6276248849623087 1103 94-95 0.6919414527899796 1103 96-97 0.7046278911771751 1103 98-99 0.6785475439614324 1103 100 0.6161470285648853 1104 1 0.25136777570388347 1104 2 0.2463974744895978 1104 3 0.26949653365404913 1104 4 0.10534836029214034 1104 5 0.14760755661335878 1104 6 0.20975058092548693 1104 7 0.20441820967064928 1104 8 0.20860640590365875 1104 9 0.26580710710513955 1104 10-11 0.25282541874583586 1104 12-13 0.26960402000160855 1104 14-15 0.38880835867275465 1104 16-17 0.2807686231630484 1104 18-19 0.326125726805472 1104 20-21 0.2142464600744418 1104 22-23 0.268433266452881 1104 24-25 0.3244554577993526 1104 26-27 0.336829640170059 1104 28-29 0.45644922153469736 1104 30-31 0.3248598287533966 1104 32-33 0.29927692998976596 1104 34-35 0.4354571995615686 1104 36-37 0.3588093458521442 1104 38-39 0.4606513725509487 1104 40-41 0.48424980660020367 1104 42-43 0.5168314501666984 1104 44-45 0.41980664765010545 1104 46-47 0.39126543801695846 1104 48-49 0.4300781253672241 1104 50-51 0.3770267304155226 1104 52-53 0.4123917900360681 1104 54-55 0.3965266555383451 1104 56-57 0.45360043005582895 1104 58-59 0.4584302605253612 1104 60-61 0.4186663394737238 1104 62-63 0.4552402213026028 1104 64-65 0.6287317772190519 1104 66-67 0.5569564994035474 1104 68-69 0.5659881090147465 1104 70-71 0.4573950007404193 1104 72-73 0.517936185701096 1104 74-75 0.6474500620820081 1104 76-77 0.5728281008390148 1104 78-79 0.6863753184091408 1104 80-81 0.6824615766021473 1104 82-83 0.5698454105932029 1104 84-85 0.6286702121614809 1104 86-87 0.5975473628655479 1104 88-89 0.6177370997766971 1104 90-91 0.5482065064084551 1104 92-93 0.5672423769487871 1104 94-95 0.516136963447849 1104 96-97 0.5191344636650079 1104 98-99 0.5339620396080704 1104 100 0.539394426662902 1105 1 0.2283499454809217 1105 2 0.1810383747452775 1105 3 0.21085794174626216 1105 4 0.10846936040181276 1105 5 0.0634175340728973 1105 6 0.16028835631757943 1105 7 0.17049310107897497 1105 8 0.1432717342477403 1105 9 0.14519144322468946 1105 10-11 0.1682498248398403 1105 12-13 0.18048112831219498 1105 14-15 0.2769562943243571 1105 16-17 0.28003633569464625 1105 18-19 0.28710913380587044 1105 20-21 0.18138341053941787 1105 22-23 0.2823554972149722 1105 24-25 0.28099858820564805 1105 26-27 0.27552218073537205 1105 28-29 0.295458125618552 1105 30-31 0.3858171953507039 1105 32-33 0.417045421071613 1105 34-35 0.4388913994024932 1105 36-37 0.4107658288705949 1105 38-39 0.3696197056259578 1105 40-41 0.3500515323549038 1105 42-43 0.3214459936405589 1105 44-45 0.3703791714655864 1105 46-47 0.4191046138868728 1105 48-49 0.4290022627674617 1105 50-51 0.48887366882007655 1105 52-53 0.4649250177881399 1105 54-55 0.49240909285315126 1105 56-57 0.41612382573048023 1105 58-59 0.5147622765773292 1105 60-61 0.4966203243170497 1105 62-63 0.4606823309159651 1105 64-65 0.5241584846640919 1105 66-67 0.5125834243893976 1105 68-69 0.46489719560958065 1105 70-71 0.5443867470582262 1105 72-73 0.5418060938098108 1105 74-75 0.5173811158558905 1105 76-77 0.4681477338579363 1105 78-79 0.5899997333556612 1105 80-81 0.6027939598359602 1105 82-83 0.5602788403257151 1105 84-85 0.5759209798742368 1105 86-87 0.5559732965516524 1105 88-89 0.5771083137842439 1105 90-91 0.6274495900728319 1105 92-93 0.6119083684713615 1105 94-95 0.6367698456322124 1105 96-97 0.6925861814570062 1105 98-99 0.5315895185608994 1105 100 0.7533392450409799 1106 1 0.1849651481805097 1106 2 0.18678280880602927 1106 3 0.18904357189658327 1106 4 0.11785062439958693 1106 5 0.11759598145932415 1106 6 0.10499000914458634 1106 7 0.1222835878238584 1106 8 0.10431570121583889 1106 9 0.10192704594832236 1106 10-11 0.1454893751920352 1106 12-13 0.14161001615094904 1106 14-15 0.1682232462236044 1106 16-17 0.21211838492101265 1106 18-19 0.2087846458031848 1106 20-21 0.264238913049617 1106 22-23 0.25620277147173454 1106 24-25 0.23352925337042052 1106 26-27 0.2375258417753443 1106 28-29 0.2561033390999654 1106 30-31 0.3253215237145426 1106 32-33 0.33640054690217625 1106 34-35 0.3324540630485444 1106 36-37 0.33919944622910236 1106 38-39 0.34738974052741156 1106 40-41 0.35081299517092646 1106 42-43 0.3262800050715313 1106 44-45 0.42683844700108864 1106 46-47 0.40022503719218605 1106 48-49 0.45360820887542985 1106 50-51 0.4394121299870335 1106 52-53 0.451439425637048 1106 54-55 0.3161006953585854 1106 56-57 0.3349597361320562 1106 58-59 0.4551542571369822 1106 60-61 0.5470452568370092 1106 62-63 0.45794524271175874 1106 64-65 0.519903648740943 1106 66-67 0.5334888808664715 1106 68-69 0.49154494222824496 1106 70-71 0.5513195237837039 1106 72-73 0.5031596752136309 1106 74-75 0.4529328002828734 1106 76-77 0.4850565070476378 1106 78-79 0.6002267618576909 1106 80-81 0.5840644884425323 1106 82-83 0.5810420241593093 1106 84-85 0.5644709499708256 1106 86-87 0.4538337906010348 1106 88-89 0.6077173341949127 1106 90-91 0.6899178453634853 1106 92-93 0.6710975496948492 1106 94-95 0.7367019352509452 1106 96-97 0.6790298786720896 1106 98-99 0.7674841247946169 1106 100 0.9157359522535664 1107 1 0.20429567016152106 1107 2 0.1883551202853866 1107 3 0.23629238427621146 1107 4 0.1506090383833154 1107 5 0.16949353428491776 1107 6 0.16335471721380657 1107 7 0.19982180670677252 1107 8 0.1833946093640293 1107 9 0.23867442350435653 1107 10-11 0.2318358930638098 1107 12-13 0.21837747793756535 1107 14-15 0.2749795659918348 1107 16-17 0.25923768371077927 1107 18-19 0.20207771168407618 1107 20-21 0.2759153468557898 1107 22-23 0.3357008801883552 1107 24-25 0.31104176201841227 1107 26-27 0.33995748004800674 1107 28-29 0.3340578713056743 1107 30-31 0.2736832730444334 1107 32-33 0.26260474831203595 1107 34-35 0.3281465675389015 1107 36-37 0.38994773780461145 1107 38-39 0.3538505713282234 1107 40-41 0.36675567812055476 1107 42-43 0.32228254474778595 1107 44-45 0.3593042592630269 1107 46-47 0.39662601676588594 1107 48-49 0.4071924356050616 1107 50-51 0.43060561834070654 1107 52-53 0.4384902664579684 1107 54-55 0.4399983174780786 1107 56-57 0.4807329185146898 1107 58-59 0.585577054388537 1107 60-61 0.5621205287055488 1107 62-63 0.5596272970227716 1107 64-65 0.62799442594347 1107 66-67 0.6043614119964786 1107 68-69 0.5303458113716744 1107 70-71 0.526134630072832 1107 72-73 0.5306111942348082 1107 74-75 0.6041538027125668 1107 76-77 0.5687792060778527 1107 78-79 0.6446550102290018 1107 80-81 0.6743864685760776 1107 82-83 0.6859969776279087 1107 84-85 0.5738588978926131 1107 86-87 0.4623119811724443 1107 88-89 0.5161080549724133 1107 90-91 0.5037390352166575 1107 92-93 0.5296931824196065 1107 94-95 0.6901387749653658 1107 96-97 0.7463073765245305 1107 98-99 0.7197340315778327 1107 100 0.9082640355294309 1108 1 0.21925647539871562 1108 2 0.12570948058395714 1108 3 0.18784012712670517 1108 4 0.15093740369787412 1108 5 0.07320972817974791 1108 6 0.12638284639134412 1108 7 0.14516880322309333 1108 8 0.1471128668556574 1108 9 0.12132989244315695 1108 10-11 0.16200481837337577 1108 12-13 0.1592715402998195 1108 14-15 0.26541968231352797 1108 16-17 0.26817228850936914 1108 18-19 0.32735957818926664 1108 20-21 0.34608782010371186 1108 22-23 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-0.1376725797802436 2316 70-71 -0.3505111623640147 2316 72-73 -0.41443281067667925 2316 74-75 -0.5366530461341696 2316 76-77 -0.43271122917439087 2316 78-79 -0.3143560827410923 2316 80-81 -0.3365336537357315 2316 82-83 -0.25555912643535095 2316 84-85 -0.26901876208915354 2316 86-87 -0.23748485133065245 2316 88-89 -0.26565409773250437 2316 90-91 -0.14826780961660546 2316 92-93 -0.12802722266182087 2316 94-95 0.0045354877268088956 2316 96-97 0.07704375615308834 2316 98-99 -0.10488548703140665 2316 100 -0.32363989754027855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 4.0 10 11.0 11 35.0 12 80.0 13 234.0 14 682.0 15 1757.0 16 4149.0 17 8371.0 18 14813.0 19 23377.0 20 32921.0 21 45478.0 22 59539.0 23 67668.0 24 72933.0 25 81458.0 26 94619.0 27 114376.0 28 138376.0 29 167025.0 30 201145.0 31 243193.0 32 297649.0 33 371573.0 34 485222.0 35 658976.0 36 963728.0 37 1495860.0 38 1497090.0 39 228374.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29248587506999 20.632878812896692 14.057648085015915 24.016987227017403 2 14.72056890877692 21.60665410389808 40.132085564401386 23.54069142292361 3 16.882695945545343 27.126278528054996 31.214379997077614 24.776645529322046 4 11.667688734282319 18.056800729376395 38.95457406914966 31.32093646719163 5 12.10895626654286 38.57167103965511 36.09219405621801 13.227178637584021 6 30.037786186164837 38.947286160590544 18.043999450254475 12.970928202990143 7 26.106727411358964 33.12545280392471 23.279575727810233 17.48824405690609 8 25.054550679185294 36.8364662692084 20.557414801831424 17.551568249774885 9 25.93405165127291 15.20001117934188 20.749509750584 38.116427418801216 10-11 24.020193359147115 26.762462113709073 29.587375216057005 19.62996931108681 12-13 24.655733345634225 24.628938207018564 29.366576490284558 21.348751957062657 14-15 22.000830310118115 26.02291628791682 26.861068223814705 25.115185178150362 16-17 21.564356295941238 28.883342448260972 27.604358233343866 21.947943022453927 18-19 21.435899711029986 28.034595540345702 29.4227953232417 21.106709425382608 20-21 22.178616802405692 27.26216861885925 29.478573820340394 21.080640758394665 22-23 21.909428361331425 27.253688232117806 29.162214377389517 21.67466902916125 24-25 21.71667737228773 27.397824519212016 29.32841804115015 21.557080067350103 26-27 21.75631474173043 27.496784917746943 29.274465253029923 21.472435087492702 28-29 21.71025215830181 27.421824414138886 29.412071566109855 21.455851861449453 30-31 21.943116799527637 27.396259632171656 29.343628453105385 21.31699511519532 32-33 21.746916145938304 27.501078450919138 29.26029494097809 21.491710462164466 34-35 21.925170003419066 27.565667721330065 29.290693635658116 21.218468639592754 36-37 21.992056050484617 27.46206198348285 29.160489569360386 21.38539239667215 38-39 21.84019890815422 27.627703327717356 29.15706889959865 21.375028864529774 40-41 21.932157463695905 27.47207339573519 29.245327882481508 21.35044125808739 42-43 21.760885951612597 27.54374702223244 29.10094095250164 21.594426073653324 44-45 21.818355486697193 27.715846560067664 28.95729152010559 21.508506433129547 46-47 21.871051894795393 27.6351337004002 28.71632813012514 21.777486274679273 48-49 21.793278124226394 27.71740358221709 28.776378286836117 21.712940006720398 50-51 21.89363776635266 27.858021501968217 28.526959412850488 21.72138131882863 52-53 21.889767828477414 27.92335377242685 28.43622719935982 21.75065119973592 54-55 21.831901003552304 27.896888536737503 28.50473861069721 21.766471849012984 56-57 21.899383409668825 27.962268405518515 28.339113556765355 21.799234628047305 58-59 21.875163871475785 27.87130130419478 28.502385833011157 21.75114899131828 60-61 22.015040601428677 27.990522353308954 28.42196341366783 21.572473631594544 62-63 21.766634324817595 27.964253945729332 28.58562859033027 21.683483139122803 64-65 21.757461854786943 27.7967514344098 28.526754054330173 21.919032656473085 66-67 21.71364306835693 27.8567694308951 28.43504803339278 21.99453946735519 68-69 21.72877797206901 27.899312695705603 28.409434602561507 21.96247472966388 70-71 21.722849516327265 27.75534510047692 28.41272456864009 22.109080814555725 72-73 21.678398058664083 27.92115391986562 28.244660155973072 22.155787865497224 74-75 21.667682198400303 28.002137704474972 28.17144726364737 22.158732833477355 76-77 21.634812049141196 27.8636084822815 28.301325402275033 22.200254066302268 78-79 21.60718205972151 27.83517603867062 28.24113836612646 22.316503535481406 80-81 21.592563456659736 28.063984046252628 28.026034468582445 22.31741802850519 82-83 21.70562279772427 28.00164054382196 28.075295397962485 22.217441260491288 84-85 21.630741300385655 27.986056978827982 28.034541110180765 22.348660610605595 86-87 21.496776077626816 27.97164601170178 28.169941545394273 22.361636365277132 88-89 21.55689203746712 27.87340765049687 28.13050540280491 22.4391949092311 90-91 21.668998973531664 27.92232712476503 28.04818620168176 22.36048770002155 92-93 21.54345285051446 27.86733508071015 28.067302847545616 22.52190922122978 94-95 21.40269428802876 27.800299962519954 28.102590140596657 22.69441560885463 96-97 21.44977931037197 27.782643090342805 28.069715002301177 22.697862596984052 98-99 21.387182052887447 27.704180511103637 28.010318634415405 22.89831880159351 100 20.71656446379793 28.096054865231984 28.147524944601475 23.03985572636861 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2093.0 1 1585.0 2 1611.5 3 2244.0 4 1848.5 5 1642.0 6 2562.0 7 5049.5 8 8077.5 9 9485.0 10 8236.5 11 6589.5 12 6666.0 13 7079.5 14 7522.0 15 8004.0 16 8623.5 17 9299.5 18 10129.5 19 11232.0 20 12758.5 21 15041.0 22 18564.5 23 23524.5 24 29324.0 25 36985.5 26 47146.0 27 58538.5 28 70634.0 29 84247.0 30 100166.0 31 116144.0 32 133201.0 33 151509.5 34 169196.5 35 186072.5 36 206077.0 37 224924.0 38 237393.5 39 247975.0 40 252755.5 41 254900.0 42 258579.5 43 262917.5 44 267618.0 45 272876.0 46 275801.5 47 275372.5 48 275079.0 49 273507.0 50 269634.0 51 258150.5 52 242503.5 53 229902.0 54 215951.0 55 197793.5 56 177508.5 57 157113.5 58 136912.0 59 115761.5 60 95019.5 61 76677.0 62 60647.0 63 46581.5 64 34617.5 65 25477.0 66 18625.5 67 13810.5 68 10254.0 69 7667.0 70 5871.5 71 4383.5 72 3391.0 73 2656.5 74 2010.5 75 1418.5 76 975.5 77 638.0 78 396.0 79 282.5 80 207.5 81 148.0 82 101.0 83 63.0 84 52.5 85 45.5 86 31.0 87 24.5 88 18.5 89 11.5 90 7.5 91 5.0 92 4.5 93 4.0 94 4.0 95 3.0 96 1.5 97 2.0 98 3.5 99 4.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0035410284449051248 2 0.06864982349126411 3 7.733280511861767E-4 4 8.140295275643965E-4 5 0.0015330889435796133 6 7.733280511861767E-4 7 0.0 8 9.632682742845359E-4 9 2.713431758547988E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.376564632587773E-4 18-19 0.0010718055446264556 20-21 0.003039043569573747 22-23 0.0 24-25 2.0350738189109913E-5 26-27 1.4923874672013936E-4 28-29 9.836190124736456E-4 30-31 0.0017365963254707124 32-33 0.0029101555610427173 34-35 0.004070147637821982 36-37 0.0013431487204812543 38-39 0.001383850196859474 40-41 0.0053386769849431675 42-43 0.006722527181802641 44-45 0.005114818864862958 46-47 0.0069396017224864805 48-49 0.008676198047957193 50-51 0.008465907086669723 52-53 0.004144767011182052 54-55 0.00234033489174764 56-57 0.002794834711304428 58-59 0.0034392747539595753 60-61 0.007543340288763408 62-63 0.01399452429471125 64-65 0.0038937745735163633 66-67 0.010351742158860576 68-69 0.012658159153626367 70-71 0.005637154478383446 72-73 0.007563691026952518 74-75 0.0029440734580245672 76-77 0.003025476410781007 78-79 0.008113160958058486 80-81 0.0058881469160491345 82-83 0.01672152321205198 84-85 0.01788829886822761 86-87 0.02290814762154139 88-89 0.021144416978485202 90-91 0.024203811286248055 92-93 0.0346301728184687 94-95 0.03825260421613027 96-97 0.02279961035119947 98-99 0.01836993300536988 100 0.019129693897763318 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7370740.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.730933800298494 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66461230515378 45.47385305634102 2 16.542773661089498 20.42401731492075 3 5.097489609385689 9.44018380874092 4 1.9647082086188823 4.85133089452621 5 0.9440069605830245 2.9137215595385837 6 0.527196493675142 1.9526599110485803 7 0.30579992354197855 1.3214120385414354 8 0.19315689845934197 0.9539004569491696 9 0.14079705646786073 0.7822380394884958 >10 0.5683916219061508 5.981000746519182 >50 0.029580332234460622 1.2691549666126838 >100 0.01925556837587374 2.2426590838353766 >500 0.001606584037624524 0.6759376523207337 >1k 5.355226890943482E-4 0.5732862908418163 >5k 0.0 0.0 >10k+ 8.925378149502963E-5 1.1446441797751399 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24768 0.3360313889785829 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22043 0.2990608812683665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15514 0.21048090151056745 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3567158792739942E-5 2 0.0 0.0 0.0 0.0 1.3567158792739942E-5 3 0.0 0.0 0.0 0.0 1.3567158792739942E-5 4 0.0 0.0 0.0 1.3567158792739942E-5 1.3567158792739942E-5 5 0.0 0.0 0.0 1.3567158792739942E-5 1.3567158792739942E-5 6 0.0 0.0 0.0 1.3567158792739942E-5 4.0701476378219826E-5 7 0.0 0.0 0.0 1.3567158792739942E-5 4.0701476378219826E-5 8 0.0 0.0 0.0 1.3567158792739942E-5 4.0701476378219826E-5 9 0.0 0.0 0.0 1.3567158792739942E-5 5.426863517095977E-5 10-11 0.0 2.0350738189109913E-5 0.0 2.7134317585479885E-5 8.140295275643965E-5 12-13 0.0 3.3917896981849854E-5 0.0 6.105221456732974E-5 1.3567158792739941E-4 14-15 0.0 6.783579396369971E-5 0.0 6.783579396369971E-5 1.9672380249472916E-4 16-17 0.0 9.49701115491796E-5 0.0 8.140295275643965E-5 2.0350738189109914E-4 18-19 0.0 9.49701115491796E-5 0.0 9.49701115491796E-5 2.102909612874691E-4 20-21 0.0 9.49701115491796E-5 0.0 1.2210442913465947E-4 2.2385812008020905E-4 22-23 0.0 9.49701115491796E-5 0.0 1.6958948490924928E-4 2.3064169947657902E-4 24-25 0.0 1.2888800853102944E-4 0.0 2.4420885826931894E-4 2.4420885826931894E-4 26-27 0.0 1.3567158792739941E-4 0.0 3.798804461967184E-4 2.7134317585479883E-4 28-29 0.0 1.8315664370198922E-4 0.0 0.001173559235572005 2.781267552511688E-4 30-31 0.0 2.1707454068383908E-4 0.0 0.0042872221785058215 2.849103346475388E-4 32-33 0.0 2.1707454068383908E-4 0.0 0.011158988107028603 2.984774934402787E-4 34-35 0.0 2.1707454068383908E-4 0.0 0.021782073441743977 3.1204465223301866E-4 36-37 0.0 2.3742527887294897E-4 0.0 0.039819611056691726 3.1204465223301866E-4 38-39 0.0 2.577760170620589E-4 0.0 0.06732024192957559 3.1204465223301866E-4 40-41 0.0 2.7134317585479883E-4 0.0 0.09965078133267488 3.1204465223301866E-4 42-43 0.0 2.9169391404390875E-4 0.0 0.1321305594824943 3.323953904221286E-4 44-45 0.0 2.984774934402787E-4 0.0 0.17332045357725276 3.527461286112385E-4 46-47 0.0 2.984774934402787E-4 0.0 0.2253572911268068 3.527461286112385E-4 48-49 0.0 2.984774934402787E-4 0.0 0.2820612313010634 3.934476049894583E-4 50-51 0.0 2.984774934402787E-4 0.0 0.3424079536111706 4.205819225749382E-4 52-53 0.0 2.984774934402787E-4 0.0 0.403819697886508 4.477162401604181E-4 54-55 0.0 3.1204465223301866E-4 0.0 0.47368378208972234 4.74850557745898E-4 56-57 0.0 3.1204465223301866E-4 0.0 0.5580308083041865 4.74850557745898E-4 58-59 0.0 3.1204465223301866E-4 0.0 0.6551580981014118 4.816341371422679E-4 60-61 0.0 3.1204465223301866E-4 0.0 0.7578479230036604 4.952012959350079E-4 62-63 0.0 3.1204465223301866E-4 0.0 0.8659849621611941 5.155520341241178E-4 64-65 0.0 3.1204465223301866E-4 0.0 0.9772560149998508 5.155520341241178E-4 66-67 0.0 3.323953904221286E-4 0.0 1.105275725368145 5.426863517095977E-4 68-69 0.0 3.3917896981849855E-4 0.0 1.257940179683451 5.562535105023376E-4 70-71 0.0 3.3917896981849855E-4 0.0 1.4267427693827215 5.766042486914475E-4 72-73 0.0 3.3917896981849855E-4 0.0 1.599913984213254 6.647907808442572E-4 74-75 0.0 3.459625492148685E-4 0.0 1.772101580031313 6.647907808442572E-4 76-77 0.0 3.527461286112385E-4 0.0 1.963378168270757 6.783579396369971E-4 78-79 0.0 3.6631328740397844E-4 0.0 2.1884776833805017 6.91925098429737E-4 80-81 0.0 3.798804461967184E-4 0.0 2.433676944241691 7.461937336006968E-4 82-83 0.0 3.798804461967184E-4 0.0 2.692456931054412 8.411638451498764E-4 84-85 0.0 3.798804461967184E-4 0.0 2.949330732056754 8.411638451498764E-4 86-87 0.0 3.798804461967184E-4 0.0 3.215830432222545 8.615145833389863E-4 88 0.0 3.798804461967184E-4 0.0 3.433183099661635 8.682981627353563E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12655 0.0 47.93062 1 GTATCAA 20825 0.0 35.177742 1 TCAACGC 25650 0.0 27.974546 4 ATCAACG 25830 0.0 27.761412 3 CAACGCA 26230 0.0 27.31977 5 TACCTGG 6115 0.0 26.450071 2 AACGCAG 27235 0.0 26.345615 6 TATCAAC 27490 0.0 26.25418 2 GTACATG 23740 0.0 24.599506 1 GTACCTG 7055 0.0 24.060003 1 TACATGG 24230 0.0 23.32469 2 ACATGGG 23495 0.0 23.100317 3 ACGCAGA 30905 0.0 23.004183 7 CGCAGAG 31030 0.0 22.79037 8 CATGGGG 12310 0.0 20.727798 4 ACCTGGG 7675 0.0 20.694252 3 GCAGAGT 35000 0.0 19.695126 9 GAGTACT 22130 0.0 19.450264 12-13 AGAGTAC 32595 0.0 19.1378 10-11 AGTACTT 23715 0.0 18.061132 12-13 >>END_MODULE