FastQCFastQC Report
Mon 6 Jun 2016
ERR1211482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173400.4901970484709895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143660.4061228834102788No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99980.2826407203352337No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89400.2527313502497489No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.1375604866124472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG65400.062.8441932
TATAACG5100.059.0121272
GTACCTG71550.058.366281
ACCTGGG69750.057.3336563
TAACGCA6050.053.5934684
GTACCCG5850.041.8500061
CCTGGGG49900.039.551864
ATAACGC8250.038.732833
GGTATCA111750.035.4320641
GTATCAA167700.031.1067541
TACCCGG6600.030.6376882
CTGGGGG34850.029.6607935
ATGGGGG55600.028.8165825
GTATAAC13900.028.7906951
CATGGGG81400.028.6335644
CCTGGGA49600.027.095834
GTACATG182850.026.8815041
TGGGGGG73550.026.5066366
TATCACG4350.025.9449862
TCAACGC198550.025.8919964
Produced by FastQC (version 0.11.5)