##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211482_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3537353
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252206946832843	31.0	31.0	34.0	28.0	34.0
2	31.2628668951049	31.0	31.0	34.0	28.0	34.0
3	31.35058955100042	33.0	31.0	34.0	28.0	34.0
4	34.88555736450391	37.0	35.0	37.0	32.0	37.0
5	34.989098910965346	37.0	35.0	37.0	32.0	37.0
6	35.108927777352164	37.0	35.0	37.0	32.0	37.0
7	35.05708336148527	37.0	35.0	37.0	32.0	37.0
8	35.02611811713448	37.0	35.0	37.0	32.0	37.0
9	36.589471562493195	39.0	37.0	39.0	32.0	39.0
10-11	36.558625899083296	39.0	37.0	39.0	32.0	39.0
12-13	36.49800429869453	39.0	37.0	39.0	32.0	39.0
14-15	37.66691661816053	40.0	38.0	41.0	32.0	41.0
16-17	37.64450635828542	40.0	37.5	41.0	32.0	41.0
18-19	37.677631551049615	40.0	38.0	41.0	32.0	41.0
20-21	37.625308670070524	40.0	37.5	41.0	32.0	41.0
22-23	37.45958362086	40.0	37.0	41.0	31.5	41.0
24-25	37.38013155599681	40.0	37.0	41.0	31.0	41.0
26-27	37.23473498404033	40.0	37.0	41.0	31.0	41.0
28-29	37.058989730456645	40.0	36.0	41.0	30.5	41.0
30-31	36.852515567431354	39.5	36.0	41.0	30.0	41.0
32-33	36.615434620180686	39.0	35.5	41.0	30.0	41.0
34-35	36.415495711058526	39.0	35.0	41.0	29.5	41.0
36-37	36.221677763005275	39.0	35.0	41.0	28.5	41.0
38-39	36.08097212802907	39.0	35.0	40.0	28.0	41.0
40-41	36.036190761849326	39.0	35.0	40.0	27.5	41.0
42-43	35.96875601615106	39.0	35.0	40.0	27.0	41.0
44-45	35.78255294283607	39.0	35.0	40.0	26.5	41.0
46-47	35.6892775473638	39.0	35.0	40.0	25.5	41.0
48-49	35.61461112306293	39.0	35.0	41.0	25.0	41.0
50-51	34.6411552649679	38.0	34.0	40.0	23.5	40.5
52-53	34.855118219753585	38.0	34.0	39.5	24.0	40.5
54-55	35.441263990334015	39.0	35.0	40.5	24.5	41.0
56-57	35.41568469417669	39.0	35.0	41.0	25.0	41.0
58-59	35.26871420522634	39.0	34.0	41.0	24.5	41.0
60-61	35.07475462584594	38.0	34.0	40.0	24.0	41.0
62-63	34.80585610200622	38.0	34.0	40.0	23.5	41.0
64-65	34.54558422639754	37.5	34.0	40.0	23.0	41.0
66-67	34.25727867702206	37.0	33.0	40.0	23.0	41.0
68-69	33.97800459835363	36.5	33.0	39.5	22.5	41.0
70-71	33.62639705452071	36.0	33.0	39.0	22.0	41.0
72-73	33.27374240003755	35.5	32.5	39.0	22.0	40.5
74-75	32.9061016811158	35.0	32.0	38.0	21.5	40.0
76-77	32.55575793538276	35.0	32.0	37.0	20.0	39.0
78-79	32.22990128494385	35.0	32.0	37.0	20.0	39.0
80-81	31.895353248601424	35.0	31.0	36.0	20.0	39.0
82-83	31.582975603509176	35.0	31.0	36.0	19.5	37.5
84-85	31.302901491595552	35.0	31.0	35.5	19.0	37.0
86-87	31.003539369692536	34.5	31.0	35.0	18.0	36.5
88-89	30.77924241657533	34.0	30.0	35.0	18.0	36.0
90-91	30.54511127388191	34.0	30.0	35.0	17.5	36.0
92-93	30.372899736045568	34.0	30.0	35.0	17.0	36.0
94-95	30.20148286020649	34.0	29.5	35.0	16.5	35.0
96-97	30.0882866369288	34.0	29.0	35.0	15.0	35.0
98-99	29.907390780620425	34.0	29.0	35.0	14.5	35.0
100	28.571831818876998	33.0	26.0	35.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2969357454880175
1101	2	0.33037911833664424
1101	3	0.24809047425576125
1101	4	0.29369427122648517
1101	5	0.22804938909836636
1101	6	0.33155642275380615
1101	7	0.24428583696298745
1101	8	0.31767068938944476
1101	9	0.3608664313434886
1101	10-11	0.22791957535736174
1101	12-13	0.28558073074561463
1101	14-15	0.3603794564960552
1101	16-17	0.3128490850501322
1101	18-19	0.24743945909239784
1101	20-21	0.2939941349643789
1101	22-23	0.3418117474446163
1101	24-25	0.38927038355994625
1101	26-27	0.3120665111973224
1101	28-29	0.4007050096022198
1101	30-31	0.46669043836030255
1101	32-33	0.3245364623132545
1101	34-35	0.374429754580909
1101	36-37	0.4288635243392065
1101	38-39	0.3866740075160209
1101	40-41	0.2698131051202637
1101	42-43	0.1942481799672251
1101	44-45	0.10000652993351622
1101	46-47	0.12428057331589315
1101	48-49	0.17242055151158553
1101	50-51	0.16314690234776918
1101	52-53	0.32686634384702984
1101	54-55	0.29460663106447527
1101	56-57	0.20856035414276164
1101	58-59	0.23765289808370227
1101	60-61	0.26930726741514377
1101	62-63	0.3205518237149505
1101	64-65	0.20891755534733392
1101	66-67	0.23143907957169318
1101	68-69	0.2680107777907992
1101	70-71	0.14847714323625638
1101	72-73	0.0770877339690017
1101	74-75	-0.013158443228071803
1101	76-77	0.08468408053438736
1101	78-79	-0.02829920044163714
1101	80-81	0.06823022079854368
1101	82-83	0.04957238203869707
1101	84-85	-0.15908911633774991
1101	86-87	-0.07963795408927865
1101	88-89	-0.05963455145835894
1101	90-91	-0.16349121431683855
1101	92-93	-0.27120436979434714
1101	94-95	-0.4534873287687873
1101	96-97	-0.46518980798009224
1101	98-99	-0.3994573549745155
1101	100	-0.36350806755768517
1102	1	0.36480967423478816
1102	2	0.4046650511041108
1102	3	0.3680165324317528
1102	4	0.2595396760723787
1102	5	0.35480802866632644
1102	6	0.36141658493376383
1102	7	0.288989775983147
1102	8	0.35821512374889863
1102	9	0.4628827645959248
1102	10-11	0.4108161510042123
1102	12-13	0.41246390886982454
1102	14-15	0.3824287377465012
1102	16-17	0.39530150550337595
1102	18-19	0.38660402287221274
1102	20-21	0.42793029586320586
1102	22-23	0.4439643943114504
1102	24-25	0.47352743557301125
1102	26-27	0.35801112575672533
1102	28-29	0.2565832848692793
1102	30-31	0.364498310416117
1102	32-33	0.355323529365144
1102	34-35	0.4543244938728037
1102	36-37	0.43749990611041767
1102	38-39	0.32290015401879657
1102	40-41	0.3790690211865382
1102	42-43	0.2712044188640377
1102	44-45	0.3553251159069575
1102	46-47	0.38251397103801565
1102	48-49	0.4248951146153672
1102	50-51	0.38677340415253525
1102	52-53	0.4520521127056796
1102	54-55	0.6231621999189159
1102	56-57	0.6264097907685624
1102	58-59	0.5440957389037564
1102	60-61	0.5256016593843853
1102	62-63	0.5436430452290111
1102	64-65	0.5637550540035932
1102	66-67	0.5464878255418526
1102	68-69	0.4854810021319764
1102	70-71	0.3710066252112725
1102	72-73	0.32996112521899335
1102	74-75	0.33852126189121634
1102	76-77	0.2420056454590096
1102	78-79	0.22230534489704468
1102	80-81	0.27150041765534993
1102	82-83	0.2615371433070095
1102	84-85	0.0828059033390609
1102	86-87	0.2882422916298637
1102	88-89	0.17155389269350962
1102	90-91	0.10262981000793658
1102	92-93	0.1519878955716898
1102	94-95	0.06685567728996489
1102	96-97	-0.04078540427457611
1102	98-99	-0.11450427234741056
1102	100	0.01288983964044732
1103	1	0.3593784755367153
1103	2	0.319753435709341
1103	3	0.32943648421681715
1103	4	0.3174345819760447
1103	5	0.25413903358148815
1103	6	0.21594214071335927
1103	7	0.28891291334983293
1103	8	0.3163448284641177
1103	9	0.2799535438116152
1103	10-11	0.2866497630405149
1103	12-13	0.2761406730865801
1103	14-15	0.38262568559139964
1103	16-17	0.348307698242742
1103	18-19	0.27570106171058484
1103	20-21	0.32233861689446996
1103	22-23	0.38119485210502546
1103	24-25	0.2774756240847296
1103	26-27	0.3633449471239203
1103	28-29	0.35785560776197656
1103	30-31	0.3801288228975608
1103	32-33	0.2754974670074901
1103	34-35	0.21799841707388623
1103	36-37	0.29344573354239856
1103	38-39	0.35240271849096416
1103	40-41	0.2111698193432403
1103	42-43	0.24409998204782113
1103	44-45	0.19460254911880526
1103	46-47	0.07665052631256941
1103	48-49	0.13238228415666242
1103	50-51	0.14493845623152168
1103	52-53	0.14403992211658334
1103	54-55	0.1187016028494412
1103	56-57	0.14180765713204835
1103	58-59	0.18695621250714822
1103	60-61	0.1680441797661416
1103	62-63	0.209291028944655
1103	64-65	0.13603376587493443
1103	66-67	0.19998402262978487
1103	68-69	0.20977942646823777
1103	70-71	0.2762569639062349
1103	72-73	0.1385361203399995
1103	74-75	0.1563776393033507
1103	76-77	0.08422859109424508
1103	78-79	-0.0557568043792287
1103	80-81	0.0603936524209594
1103	82-83	0.10174549840346714
1103	84-85	0.15127930751828345
1103	86-87	0.0632491006199416
1103	88-89	0.11832692401810263
1103	90-91	0.014183998096942929
1103	92-93	-0.12078615177029306
1103	94-95	-0.032089374632462864
1103	96-97	-0.07776061604159068
1103	98-99	-0.11620894923957081
1103	100	-0.16310568329673814
1104	1	0.5444486631054843
1104	2	0.5200715062020613
1104	3	0.542166807291558
1104	4	0.5131923848246416
1104	5	0.3820566442155098
1104	6	0.4580158410210302
1104	7	0.42870416596321803
1104	8	0.4545683992116665
1104	9	0.5187358612001205
1104	10-11	0.4772366967913442
1104	12-13	0.5012337613028279
1104	14-15	0.48628682662866396
1104	16-17	0.5213617668158861
1104	18-19	0.5111231484501602
1104	20-21	0.5231138109002771
1104	22-23	0.5976358638449852
1104	24-25	0.6722614802264744
1104	26-27	0.6911383509697231
1104	28-29	0.6866932969151804
1104	30-31	0.6096966457357311
1104	32-33	0.5059888324856772
1104	34-35	0.6335179392338546
1104	36-37	0.6700017875315964
1104	38-39	0.6441055672756022
1104	40-41	0.6289680709519629
1104	42-43	0.4014836519548268
1104	44-45	0.4610874007123229
1104	46-47	0.6134367142876656
1104	48-49	0.745985418557396
1104	50-51	0.7173299548509888
1104	52-53	0.7575474253822563
1104	54-55	0.7926037791532607
1104	56-57	0.7609224886584087
1104	58-59	0.709238601351494
1104	60-61	0.7540980238401787
1104	62-63	0.7939042869375967
1104	64-65	0.7046365996729023
1104	66-67	0.641934418960652
1104	68-69	0.48982052645634155
1104	70-71	0.5397778187496058
1104	72-73	0.7127457808367694
1104	74-75	0.6531458085001063
1104	76-77	0.6459877131175062
1104	78-79	0.5993147654941282
1104	80-81	0.5692606648889189
1104	82-83	0.5752827583333726
1104	84-85	0.48596507464282723
1104	86-87	0.467002682858606
1104	88-89	0.45541009013626876
1104	90-91	0.45798285064369537
1104	92-93	0.5281217573860104
1104	94-95	0.5336658916504256
1104	96-97	0.5805692336657167
1104	98-99	0.4851626286119597
1104	100	0.39606026340569045
1105	1	0.343840619198442
1105	2	0.3359286025059802
1105	3	0.2997722956716302
1105	4	0.3637324071211836
1105	5	0.3577995136092156
1105	6	0.3302539681113359
1105	7	0.3814612193891662
1105	8	0.2948203158568887
1105	9	0.4235585693726591
1105	10-11	0.4013683836106523
1105	12-13	0.43542818716682063
1105	14-15	0.4630303992210614
1105	16-17	0.5256701451710839
1105	18-19	0.3992090072958874
1105	20-21	0.4388347610760803
1105	22-23	0.5612501679355475
1105	24-25	0.488324527992539
1105	26-27	0.511902299173137
1105	28-29	0.5348580512244183
1105	30-31	0.510515340530354
1105	32-33	0.47682924068394783
1105	34-35	0.560941316302717
1105	36-37	0.44891361327552914
1105	38-39	0.5230937158048832
1105	40-41	0.43844825275455435
1105	42-43	0.4717581052607116
1105	44-45	0.5544680027190054
1105	46-47	0.44104834279294636
1105	48-49	0.45546686954787674
1105	50-51	0.3964490012449531
1105	52-53	0.4294315519580181
1105	54-55	0.4426122518399964
1105	56-57	0.4976914107062669
1105	58-59	0.571780681790564
1105	60-61	0.6305931443272712
1105	62-63	0.531017707741988
1105	64-65	0.5213141401925796
1105	66-67	0.40598045274849426
1105	68-69	0.44693998447881
1105	70-71	0.3904626999776255
1105	72-73	0.41022654577974293
1105	74-75	0.37929114766483707
1105	76-77	0.3063379403707387
1105	78-79	0.33412299806423107
1105	80-81	0.37304558899490914
1105	82-83	0.435495398758718
1105	84-85	0.31883539451247955
1105	86-87	0.2740034546620542
1105	88-89	0.3797169248845762
1105	90-91	0.4119041609944354
1105	92-93	0.32847325382713777
1105	94-95	0.3587786264073074
1105	96-97	0.33367586259977244
1105	98-99	0.35640746181666216
1105	100	0.35371185417834994
1106	1	0.3213401320724856
1106	2	0.29594377300006514
1106	3	0.26841585865005513
1106	4	0.26733894852689133
1106	5	0.2710151016398328
1106	6	0.26628069044953406
1106	7	0.3228669257218044
1106	8	0.27097605698423877
1106	9	0.2998047767491414
1106	10-11	0.3654945492334747
1106	12-13	0.4013099547381458
1106	14-15	0.450677859207147
1106	16-17	0.44132428574172167
1106	18-19	0.40406885419915994
1106	20-21	0.31406162259312964
1106	22-23	0.5461570572883616
1106	24-25	0.570775537039161
1106	26-27	0.5596230368210158
1106	28-29	0.5085087819133491
1106	30-31	0.5920615479068303
1106	32-33	0.6219301453464325
1106	34-35	0.5609044339088456
1106	36-37	0.655478609796063
1106	38-39	0.4871708904743315
1106	40-41	0.570709700214536
1106	42-43	0.4867359758243879
1106	44-45	0.5392502574162918
1106	46-47	0.5866823720204977
1106	48-49	0.6589276211136337
1106	50-51	0.6750416247661803
1106	52-53	0.6552902158411058
1106	54-55	0.6617478816242652
1106	56-57	0.6383989263638767
1106	58-59	0.5945490186033737
1106	60-61	0.5625028868464099
1106	62-63	0.6413703869347458
1106	64-65	0.7104734303804747
1106	66-67	0.704629652470139
1106	68-69	0.7101689336785313
1106	70-71	0.6326173276742253
1106	72-73	0.5848807559397926
1106	74-75	0.6766407649230217
1106	76-77	0.6217359918669132
1106	78-79	0.530154939609119
1106	80-81	0.47230654932894467
1106	82-83	0.4777757049521796
1106	84-85	0.5028655963218043
1106	86-87	0.5569804901526609
1106	88-89	0.6367009193174269
1106	90-91	0.5773155736597104
1106	92-93	0.47467916892804496
1106	94-95	0.6413425094970755
1106	96-97	0.6874310470117422
1106	98-99	0.5434467798403233
1106	100	0.48073454033005447
1107	1	0.11088365013344514
1107	2	0.16553680549520422
1107	3	0.12309061336366867
1107	4	0.14674234803185016
1107	5	0.11328675726580428
1107	6	0.16682623088505721
1107	7	0.11236146269817482
1107	8	0.1327615045951589
1107	9	0.05487132571607134
1107	10-11	0.1190961555953578
1107	12-13	0.1317304464647009
1107	14-15	0.1975550429471653
1107	16-17	0.2240782091265956
1107	18-19	0.16440386304841326
1107	20-21	0.3102098045241135
1107	22-23	0.30996591350383795
1107	24-25	0.23023332187505474
1107	26-27	0.29496667178349156
1107	28-29	0.30705325456122523
1107	30-31	0.31408586538262995
1107	32-33	0.22047192403465488
1107	34-35	0.21799275877751256
1107	36-37	0.24619008181915092
1107	38-39	0.25613486978472366
1107	40-41	0.2702821769811834
1107	42-43	0.2156605035923107
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2312	62-63	-0.34031001458514254
2312	64-65	-1.0020313143528696
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2312	98-99	-0.33103485452179626
2312	100	-0.43938653625416535
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2313	82-83	0.047856937220259965
2313	84-85	-0.0613150328379497
2313	86-87	-0.5886685538849541
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2313	98-99	0.32116600882520885
2313	100	0.30472125588775256
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2314	2	-0.24877488821766391
2314	3	-0.31288371417625527
2314	4	-0.162300266755544
2314	5	-0.5457296167873409
2314	6	-0.3389818008150698
2314	7	-0.2853197530895102
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2314	82-83	-0.73708200386152
2314	84-85	-0.2622508975828346
2314	86-87	-0.7901790829269721
2314	88-89	-0.580812926517865
2314	90-91	-0.6677111735698809
2314	92-93	-0.8044387658686283
2314	94-95	-0.8256338241334475
2314	96-97	-0.6479793910712033
2314	98-99	-0.5158248427643635
2314	100	-0.31342729995864005
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2315	2	-0.15628043342429265
2315	3	-0.20495114794274372
2315	4	-0.03021181201791734
2315	5	-0.1180018382228738
2315	6	-0.18178402548524986
2315	7	-0.3538998620765028
2315	8	-0.1383058314216541
2315	9	-0.24372268285074483
2315	10-11	-0.3874753019105128
2315	12-13	-0.2927277004176716
2315	14-15	-0.2336707035893184
2315	16-17	-0.16287129915478005
2315	18-19	-0.06005787281794994
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2315	22-23	-0.04252656632272789
2315	24-25	-0.06817358655744243
2315	26-27	-0.1644542999019265
2315	28-29	-0.2349673489890307
2315	30-31	-0.02850700027363473
2315	32-33	-0.1888039859258086
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2315	84-85	0.19509978440931164
2315	86-87	0.20504721133227832
2315	88-89	0.0956482660443072
2315	90-91	-0.10359260172345941
2315	92-93	-0.03441141355101607
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2315	96-97	0.23585074698255326
2315	98-99	0.3238617485473618
2315	100	0.19892523020111952
2316	1	-0.4277869319114984
2316	2	-0.6683917625023561
2316	3	-0.44825746654649734
2316	4	-0.21830974432217687
2316	5	-0.2184218779850653
2316	6	-0.31323094897211234
2316	7	-0.39117192968515013
2316	8	-0.1862953461594259
2316	9	-0.5776869513784106
2316	10-11	-0.4356649760454019
2316	12-13	-0.3836972318030831
2316	14-15	-0.3853440501954495
2316	16-17	-0.4362917746323731
2316	18-19	-0.3570714524742229
2316	20-21	-0.17234309274510196
2316	22-23	-0.15791213793442438
2316	24-25	-0.12545650210310555
2316	26-27	-0.354675466905654
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2316	30-31	-0.23461088566952526
2316	32-33	-0.3326109628783982
2316	34-35	-0.33702902677002555
2316	36-37	-0.27394254185301037
2316	38-39	-0.24812867605261602
2316	40-41	-0.15944828142110623
2316	42-43	-0.2703776146655841
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2316	46-47	-0.26327721656478076
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2316	50-51	-0.1065916585778055
2316	52-53	-0.46234186209831307
2316	54-55	-0.5304032434657913
2316	56-57	-0.39649169010974106
2316	58-59	-0.4633291190342135
2316	60-61	-0.44003414355550063
2316	62-63	-0.5250522404900053
2316	64-65	-0.3920502188492705
2316	66-67	-0.22229379486052636
2316	68-69	-0.37377511598622704
2316	70-71	-0.38788272425681924
2316	72-73	-0.4724255907474131
2316	74-75	-0.186956250773342
2316	76-77	-0.24582385659337547
2316	78-79	-0.4985880311539823
2316	80-81	-0.23791097852285503
2316	82-83	-0.16228903574158693
2316	84-85	-0.11847807394413223
2316	86-87	-0.09412605310314603
2316	88-89	-0.15597966193651303
2316	90-91	0.0027190421035037105
2316	92-93	-0.010695615173474948
2316	94-95	-0.03267080765428787
2316	96-97	-0.10469237465564518
2316	98-99	0.01784275180525441
2316	100	-0.13709190119586978
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13.0
1	28.0
2	47.0
3	56.0
4	67.0
5	66.0
6	383.0
7	4259.0
8	6594.0
9	5779.0
10	5950.0
11	7192.0
12	9097.0
13	10461.0
14	10802.0
15	11820.0
16	13787.0
17	17584.0
18	22391.0
19	26138.0
20	29922.0
21	34240.0
22	38811.0
23	39748.0
24	37838.0
25	37402.0
26	39194.0
27	43826.0
28	50467.0
29	59021.0
30	70504.0
31	86303.0
32	108654.0
33	142393.0
34	200326.0
35	277829.0
36	419539.0
37	694887.0
38	771566.0
39	202005.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.830866271226945	17.883614094024345	12.209097529842031	23.076422104906676
2	15.905738781202317	20.67539742910571	41.71647264972358	21.702391139968398
3	17.908460691203647	28.946962852363846	30.288762673928503	22.855813782504004
4	11.520326311401838	17.104795074109497	39.04264229888464	32.33223631560402
5	11.623832386838409	37.3081690010211	34.64038518474112	16.42761342739937
6	27.13982250648525	36.751199234660994	21.67550214064235	14.433476118211406
7	25.83767456942675	33.08068550207607	23.993222893907983	17.08841703458919
8	26.11177752942976	34.452077392228944	22.077174539622423	17.358970538718875
9	27.943926686842957	15.80482054080186	21.39708324910839	34.85416952324679
10-11	24.1082418995977	26.038881365611495	30.103373600929554	19.74950313386125
12-13	24.776166916850446	24.545495539877464	29.504675696654274	21.17366184661781
14-15	22.405801551668514	25.595970717409887	28.16094001513837	23.83728771578323
16-17	21.296777838829883	27.655859689914042	28.83941610689016	22.207946364365913
18-19	20.8665708060842	26.89948224823838	30.835783923216155	21.398163022461265
20-21	22.047805483417967	26.692377478143474	30.549225402779385	20.710591635659167
22-23	21.422648246026863	26.65248106197734	30.337829888136696	21.587040803859104
24-25	20.900424594412954	26.608345371095293	30.597502761687373	21.893727272804384
26-27	20.82838920096884	26.584362942713657	30.58643134999764	22.000816506319868
28-29	20.899863152392584	27.157258529151907	30.541264169891804	21.40161414856371
30-31	22.199479025444514	26.674711528647236	30.590828009125676	20.53498143678257
32-33	21.559865911690654	26.689842249297318	30.670558890263866	21.079732948748166
34-35	21.693354779394873	27.12527156897771	30.299626098275347	20.881747553352074
36-37	21.571012312655782	27.248024588553143	30.089167436001475	21.091795662789597
38-39	21.484478989459348	27.6964741832046	29.714815595530393	21.104231231805656
40-41	21.679086445677264	27.044428150543954	30.18710399671533	21.089381407063453
42-43	21.275611981206616	27.273214450341023	29.646382578960434	21.804790989491927
44-45	21.059216479183192	27.614574715133667	29.870527450434466	21.455681355248675
46-47	21.044656305835936	26.94838161855958	29.63276196357564	22.37420011202885
48-49	20.978441373856498	27.224789684611384	29.854373495588852	21.942395445943266
50-51	21.199026047738023	27.605261513589895	29.691237794142882	21.504474644529196
52-53	21.290914648474114	27.89967818819473	28.968915654542478	21.840491508788677
54-55	21.080243614562356	27.623044412886983	29.532638503610727	21.764073468939934
56-57	21.35292476013311	27.733852016414406	28.949268078341955	21.96395514511053
58-59	21.24266562606923	27.906122841396552	29.26413077595202	21.587080756582203
60-61	22.044001983946323	28.03807333249255	29.11610893154763	20.801815752013496
62-63	21.626773566184664	27.84903375220476	29.358537838152788	21.165654843457787
64-65	21.36381226980281	27.326949047072528	29.180092553251207	22.129146129873455
66-67	21.2142646083722	27.598884234367333	29.017328863861607	22.169522293398856
68-69	20.96088342196783	27.810904467741288	29.250330744545312	21.97788136574557
70-71	21.120981920453843	27.49543850390564	29.453187518179874	21.93039205746064
72-73	20.929028641743265	28.04498548547409	28.85287521099408	22.173110661788566
74-75	21.332519763593414	27.96440467411371	28.74661945511096	21.95645610718192
76-77	21.04812675152698	27.51245066882847	29.246085953089402	22.193336626555148
78-79	20.986739935491578	27.629425057909845	29.16778763539099	22.216047371207587
80-81	21.091795066031636	27.95900325305254	28.610165149428486	22.33903653148734
82-83	21.53226712849241	28.150098079064094	28.566023998444873	21.751610793998623
84-85	21.375795735305513	28.482927140985325	28.16183377225896	21.979443351450207
86-87	21.05090825035009	28.32822132509757	28.652557571062097	21.968312853490243
88-89	20.857984150339917	27.98036435896189	28.874961405048506	22.286690085649692
90-91	21.81768592987098	27.866374218527646	28.70195244904108	21.61398740256029
92-93	21.354550692262286	28.07401568680124	28.695478822111394	21.87595479882508
94-95	21.215494983022733	27.913502117757766	28.551367165713575	22.319635733505926
96-97	21.23920515076599	27.97404033846857	28.57509403789563	22.211660472869813
98-99	21.257713431323317	27.737080568034273	28.470789620119163	22.534416380523247
100	21.197470358503516	27.986301404731044	28.562883413460522	22.25334482330492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2039.0
1	1505.0
2	3311.0
3	4987.5
4	3120.0
5	2022.0
6	2515.5
7	4433.0
8	6921.0
9	7915.0
10	6797.5
11	5395.5
12	5276.0
13	5577.0
14	5904.0
15	6237.0
16	6601.5
17	6998.5
18	7458.0
19	7999.0
20	8674.5
21	9664.0
22	11060.5
23	13101.0
24	15733.5
25	18972.0
26	23334.0
27	28377.5
28	33482.0
29	40040.5
30	47992.0
31	55079.5
32	63559.0
33	73809.0
34	83984.0
35	92368.5
36	101661.0
37	110523.0
38	115110.5
39	118999.0
40	118517.5
41	116660.0
42	116204.5
43	115531.5
44	116323.0
45	118349.5
46	120116.0
47	120084.5
48	123113.5
49	128903.5
50	135048.5
51	132623.5
52	120804.5
53	110718.0
54	103115.0
55	94131.5
56	84549.5
57	76883.5
58	68402.5
59	57505.0
60	44168.5
61	32681.0
62	25348.0
63	19328.0
64	14279.0
65	10632.0
66	8071.5
67	6387.5
68	5059.0
69	4058.5
70	3345.5
71	2745.5
72	2290.5
73	1945.0
74	1664.5
75	1466.5
76	1301.0
77	1103.5
78	957.5
79	855.5
80	779.0
81	711.0
82	606.5
83	505.0
84	443.0
85	367.5
86	274.5
87	225.5
88	179.5
89	118.5
90	85.5
91	63.5
92	44.5
93	30.5
94	23.5
95	17.5
96	12.5
97	15.5
98	12.0
99	10.5
100	21.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16274881245948594
2	0.1029300722885163
3	0.02990937008548482
4	0.031068428850612312
5	0.027478173651314983
6	0.0027421634199357543
7	0.0042404588968078675
8	0.005710484647701262
9	0.005597405743786385
10-11	0.005173359854105598
12-13	0.008099276492903026
14-15	0.01820570353029511
16-17	0.008523322382583813
18-19	0.01751309524381649
20-21	0.020029100855922494
22-23	0.04548598909975905
24-25	0.01600066490395502
26-27	0.009385549024934747
28-29	0.026502868105049173
30-31	0.01470025750893394
32-33	0.020594495375496874
34-35	0.020594495375496874
36-37	0.0211457550320819
38-39	0.02011391003385865
40-41	0.02612122680433646
42-43	0.034347717064143725
44-45	0.038234804386217604
46-47	0.021598070647741403
48-49	0.013074748265157593
50-51	0.011986363814976905
52-53	0.02301155694667736
54-55	0.026771430501847
56-57	0.019901887089018258
58-59	0.026092957078357744
60-61	0.027336825021421384
62-63	0.05147917100724751
64-65	0.03161968850719733
66-67	0.021711149551656282
68-69	0.028651367279431825
70-71	0.042588342186940346
72-73	0.03993098794494075
74-75	0.04227737520117444
76-77	0.022502701879060415
78-79	0.017117319080114424
80-81	0.023421467973368786
82-83	0.018813502638837572
84-85	0.01942130174738003
86-87	0.0210750807171351
88-89	0.01755549983278457
90-91	0.028255591115729756
92-93	0.02658767728298533
94-95	0.02011391003385865
96-97	0.013597738195763896
98-99	0.01879936777584821
100	0.042856904583738184
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3537353.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.21912392241703
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.708065138648	51.98490177893572
2	13.983259671018832	18.239518906470273
3	3.324467580217904	6.504565892708682
4	1.1164143471609025	2.9124626262500484
5	0.536879519256447	1.7507405948896948
6	0.3096676848363246	1.2117753067264938
7	0.19514748801060178	0.8909143742598279
8	0.13757365728631699	0.717794672241315
9	0.10166924124472121	0.5967700959453827
>10	0.5136615925459831	6.0014214798616266
>50	0.04122755052838125	1.8667976945046028
>100	0.028571871372521134	3.588135743720152
>500	0.002482660235998093	1.111014463537447
>1k	7.09331495547285E-4	0.9496923343788121
>5k	1.0133307079246927E-4	0.6257923948665951
>10k+	1.0133307079246927E-4	1.0477016407033617
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17340	0.4901970484709895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14366	0.4061228834102788	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9998	0.2826407203352337	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8940	0.2527313502497489	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4866	0.1375604866124472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.8269725978719116E-5	0.0
5	0.0	0.0	0.0	2.8269725978719116E-5	0.0
6	0.0	0.0	0.0	2.8269725978719116E-5	0.0
7	0.0	0.0	0.0	2.8269725978719116E-5	0.0
8	0.0	0.0	0.0	5.653945195743823E-5	0.0
9	0.0	0.0	0.0	8.480917793615735E-5	0.0
10-11	0.0	0.0	0.0	8.480917793615735E-5	0.0
12-13	0.0	2.8269725978719116E-5	0.0	8.480917793615735E-5	0.0
14-15	0.0	2.8269725978719116E-5	0.0	8.480917793615735E-5	0.0
16-17	0.0	2.8269725978719116E-5	0.0	8.480917793615735E-5	5.653945195743823E-5
18-19	0.0	2.8269725978719116E-5	0.0	9.894404092551692E-5	9.89440409255169E-5
20-21	0.0	2.8269725978719116E-5	0.0	1.2721376690423601E-4	1.4134862989359558E-4
22-23	0.0	4.2404588968078676E-5	0.0	2.2615780782975293E-4	1.4134862989359558E-4
24-25	0.0	5.653945195743823E-5	0.0	4.381807526701463E-4	1.4134862989359558E-4
26-27	0.0	5.653945195743823E-5	0.0	8.056871903934948E-4	1.4134862989359558E-4
28-29	0.0	5.653945195743823E-5	0.0	0.001696183558723147	1.4134862989359558E-4
30-31	0.0	5.653945195743823E-5	0.0	0.005187494717094958	1.5548349288295514E-4
32-33	0.0	5.653945195743823E-5	0.0	0.014459964838114826	1.696183558723147E-4
34-35	0.0	5.653945195743823E-5	0.0	0.028510018649538227	1.696183558723147E-4
36-37	0.0	5.653945195743823E-5	0.0	0.05128128292539648	1.696183558723147E-4
38-39	0.0	5.653945195743823E-5	0.0	0.09109919196642235	1.696183558723147E-4
40-41	0.0	5.653945195743823E-5	0.0	0.14763864392386059	1.696183558723147E-4
42-43	2.8269725978719116E-5	5.653945195743823E-5	0.0	0.19908954520512936	1.696183558723147E-4
44-45	2.8269725978719116E-5	5.653945195743823E-5	0.0	0.2569576742835674	1.696183558723147E-4
46-47	2.8269725978719116E-5	5.653945195743823E-5	0.0	0.322515168828217	1.696183558723147E-4
48-49	2.8269725978719116E-5	7.067431494679779E-5	0.0	0.3976843702056312	1.696183558723147E-4
50-51	2.8269725978719116E-5	8.480917793615735E-5	0.0	0.4850802280688413	1.696183558723147E-4
52-53	2.8269725978719116E-5	8.480917793615735E-5	0.0	0.5799251587274439	1.8375321886167427E-4
54-55	2.8269725978719116E-5	8.480917793615735E-5	0.0	0.6954069893505115	1.978880818510338E-4
56-57	2.8269725978719116E-5	8.480917793615735E-5	0.0	0.8188184781106098	1.978880818510338E-4
58-59	2.8269725978719116E-5	8.480917793615735E-5	0.0	0.9572270565024186	1.978880818510338E-4
60-61	2.8269725978719116E-5	8.480917793615735E-5	0.0	1.1008796690632798	1.978880818510338E-4
62-63	2.8269725978719116E-5	8.480917793615735E-5	0.0	1.2636567512487444	1.978880818510338E-4
64-65	5.653945195743823E-5	8.480917793615735E-5	0.0	1.424935537957337	1.978880818510338E-4
66-67	5.653945195743823E-5	8.480917793615735E-5	0.0	1.6068088200414263	1.978880818510338E-4
68-69	5.653945195743823E-5	8.480917793615735E-5	0.0	1.8273974918533717	1.978880818510338E-4
70-71	5.653945195743823E-5	8.480917793615735E-5	0.0	2.080764910937642	1.978880818510338E-4
72-73	5.653945195743823E-5	8.480917793615735E-5	0.0	2.3120395391695427	2.2615780782975293E-4
74-75	5.653945195743823E-5	8.480917793615735E-5	0.0	2.55289760450823	2.5442753380847203E-4
76-77	5.653945195743823E-5	8.480917793615735E-5	0.0	2.8218981820587317	2.8269725978719116E-4
78-79	5.653945195743823E-5	8.480917793615735E-5	0.0	3.1383212249385344	2.8269725978719116E-4
80-81	5.653945195743823E-5	8.480917793615735E-5	0.0	3.4388990864072655	2.8269725978719116E-4
82-83	5.653945195743823E-5	8.480917793615735E-5	0.0	3.7618665708511423	2.8269725978719116E-4
84-85	5.653945195743823E-5	8.480917793615735E-5	0.0	4.114121491408971	2.8269725978719116E-4
86-87	5.653945195743823E-5	8.480917793615735E-5	0.0	4.477118907838714	2.8269725978719116E-4
88	5.653945195743823E-5	8.480917793615735E-5	0.0	4.765964833026277	2.8269725978719116E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	6540	0.0	62.844193	2
TATAACG	510	0.0	59.012127	2
GTACCTG	7155	0.0	58.36628	1
ACCTGGG	6975	0.0	57.333656	3
TAACGCA	605	0.0	53.593468	4
GTACCCG	585	0.0	41.850006	1
CCTGGGG	4990	0.0	39.55186	4
ATAACGC	825	0.0	38.73283	3
GGTATCA	11175	0.0	35.432064	1
GTATCAA	16770	0.0	31.106754	1
TACCCGG	660	0.0	30.637688	2
CTGGGGG	3485	0.0	29.660793	5
ATGGGGG	5560	0.0	28.816582	5
GTATAAC	1390	0.0	28.790695	1
CATGGGG	8140	0.0	28.633564	4
CCTGGGA	4960	0.0	27.09583	4
GTACATG	18285	0.0	26.881504	1
TGGGGGG	7355	0.0	26.506636	6
TATCACG	435	0.0	25.944986	2
TCAACGC	19855	0.0	25.891996	4
>>END_MODULE