FastQCFastQC Report
Mon 6 Jun 2016
ERR1211482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3537353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191210.5405454304390882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164210.4642171702965466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120000.3392367117446294No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89670.2534946328511743No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49600.14021784085444683No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45150.1276378127939168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63750.064.907392
GTACCTG70000.059.4471051
ACCTGGG68050.059.1158453
TAACGCA7350.054.348794
TATAACG7150.053.926152
CCTGGGG46450.042.898144
ATAACGC9950.039.6747783
GGTATCA122700.033.6078341
TATCACG4150.032.858032
GTATAAC16450.031.7279911
GTACCCG6850.031.5756841
TACCCGG6800.031.1168232
CTGGGGG33150.030.4790155
GTATCAA179450.028.8751071
TGGGGGG60900.028.3968666
CCTGGGA49850.027.3395354
CATGGGG78350.026.8118524
ATGGGGG52550.026.5601025
ATCACGC5150.026.4636083
TCAACGC214600.023.5635574
Produced by FastQC (version 0.11.5)