FastQCFastQC Report
Mon 6 Jun 2016
ERR1211481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5791093
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200230.34575511047741764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177930.30724769918217515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111550.19262339596342176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92950.051.1699141
GTATCAA168000.038.8224681
TACCTGG51700.032.6535572
TCAACGC199700.031.7668784
ATCAACG201100.031.4769883
CAACGCA203600.031.1795755
GTACCTG57800.030.389511
AACGCAG210850.030.1893186
TATCAAC210750.030.1226232
GTACATG182700.027.7600231
ACGCAGA236950.026.7265727
TACATGG185650.026.5456222
CGCAGAG239900.026.2423928
ACATGGG183150.025.4531973
ACCTGGG66200.024.27763
CATGGGG102900.023.335894
GCAGAGT268700.022.8188749
GAGTACT175450.020.58098812-13
CAGAGTA261900.020.03876110-11
AGAGTAC246800.019.87506510-11
Produced by FastQC (version 0.11.5)