FastQCFastQC Report
Mon 6 Jun 2016
ERR1211481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5791093
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221080.3817586766435973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206810.3571173869941305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138980.23998923864631425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107000.050.9856531
GTATCAA181700.037.1424671
TACCTGG52750.031.7393152
TCAACGC225300.029.3046324
ATCAACG226600.029.1367633
CAACGCA228850.028.8705835
GTACCTG58400.028.8299541
TATCAAC236100.028.1459672
AACGCAG237500.027.9773716
ACCTGGG60800.026.2784793
GTACATG199400.025.236691
ACGCAGA267650.024.720467
TACATGG205500.024.0296522
CGCAGAG274450.023.988118
ACATGGG199050.023.5609743
CATGGGG111850.021.8888474
TAACGCA5650.021.6244984
GCAGAGT301450.021.4027189
GAGTACT192100.020.65806812-13
AGAGTAC278300.020.25352710-11
Produced by FastQC (version 0.11.5)