##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211481_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5791093 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13189841710364 33.0 31.0 34.0 30.0 34.0 2 32.351734810682544 34.0 31.0 34.0 31.0 34.0 3 32.367201666421174 34.0 31.0 34.0 30.0 34.0 4 35.99812608776961 37.0 35.0 37.0 35.0 37.0 5 35.92352773474714 37.0 35.0 37.0 35.0 37.0 6 35.941604460505125 37.0 35.0 37.0 35.0 37.0 7 35.87106561749224 37.0 35.0 37.0 35.0 37.0 8 35.86783962889216 37.0 35.0 37.0 35.0 37.0 9 37.52849902427746 39.0 37.0 39.0 35.0 39.0 10-11 37.49093055490561 39.0 37.0 39.0 35.0 39.0 12-13 37.4088857664693 39.0 37.0 39.0 34.0 39.0 14-15 38.65224846155985 40.0 38.0 41.0 34.0 41.0 16-17 38.581729562968505 40.0 38.0 41.0 34.0 41.0 18-19 38.57671185732987 40.0 38.0 41.0 34.0 41.0 20-21 38.52022873747667 40.0 38.0 41.0 34.0 41.0 22-23 38.41010237618356 40.0 38.0 41.0 34.0 41.0 24-25 38.30173906721926 40.0 38.0 41.0 34.0 41.0 26-27 38.13087304935355 40.0 38.0 41.0 33.0 41.0 28-29 38.1629961736066 40.0 38.0 41.0 33.5 41.0 30-31 38.07919791652456 40.0 37.5 41.0 33.0 41.0 32-33 37.88980163157456 40.0 37.0 41.0 33.0 41.0 34-35 37.7671360138751 40.0 37.0 41.0 33.0 41.0 36-37 37.702719762918676 40.0 37.0 41.0 33.0 41.0 38-39 37.603247953365624 40.0 37.0 41.0 32.5 41.0 40-41 37.4420055937627 40.0 37.0 41.0 32.0 41.0 42-43 37.290692620546764 40.0 36.0 41.0 31.0 41.0 44-45 37.103904910523795 40.0 36.0 41.0 31.0 41.0 46-47 36.84923839074938 39.0 36.0 41.0 30.0 41.0 48-49 36.62375530836752 39.0 35.5 41.0 30.0 41.0 50-51 36.42182477470142 39.0 35.0 41.0 29.5 41.0 52-53 36.22904933490103 39.0 35.0 40.0 28.5 41.0 54-55 36.036357558063735 39.0 35.0 40.0 28.0 41.0 56-57 35.84402840707273 38.0 35.0 40.0 28.0 41.0 58-59 35.592842663725136 38.0 34.0 40.0 27.0 41.0 60-61 35.285931343185126 38.0 34.0 40.0 26.5 41.0 62-63 34.9697159068245 37.5 34.0 40.0 26.0 41.0 64-65 34.664838399245184 37.0 33.5 40.0 26.0 41.0 66-67 34.332885001156086 36.5 33.0 39.0 26.0 41.0 68-69 33.9441831619696 36.0 32.5 39.0 25.0 40.0 70-71 33.55652387209116 35.5 32.0 39.0 24.5 40.0 72-73 33.150922891412726 35.0 32.0 38.0 24.0 40.0 74-75 32.7419002768562 35.0 31.0 37.0 23.0 39.5 76-77 31.200108166109572 33.5 29.5 35.5 21.0 38.5 78-79 32.0175400395055 34.0 31.0 36.0 22.5 39.0 80-81 31.970729791422794 34.5 31.0 36.0 22.5 38.0 82-83 31.684842049678707 34.0 31.0 35.5 21.5 37.0 84-85 31.37610637577397 34.0 30.5 35.0 20.5 37.0 86-87 31.070837232280674 34.0 30.0 35.0 20.0 36.0 88-89 30.789332169246805 34.0 30.0 35.0 20.0 36.0 90-91 30.50402635564651 34.0 29.5 35.0 19.0 36.0 92-93 30.292148217961618 34.0 29.0 35.0 19.0 35.0 94-95 30.07142157793011 34.0 29.0 35.0 18.0 35.0 96-97 29.863419047147058 34.0 29.0 35.0 18.0 35.0 98-99 29.701637065749075 34.0 29.0 35.0 17.5 35.0 100 27.809939678053865 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27793529380384285 1101 2 0.21286379913343723 1101 3 0.2732269315514344 1101 4 0.14967150719017752 1101 5 0.16182260481769362 1101 6 0.1555382735197668 1101 7 0.19139574369996382 1101 8 0.19779356674445836 1101 9 0.2027225654123086 1101 10-11 0.21372526810758075 1101 12-13 0.23294024778605404 1101 14-15 0.3003333969431665 1101 16-17 0.2749655253423171 1101 18-19 0.3042531499200436 1101 20-21 0.26787419153291836 1101 22-23 0.285218705630669 1101 24-25 0.2398243263704103 1101 26-27 0.19385873583834723 1101 28-29 0.23638200581546442 1101 30-31 0.3197905565682646 1101 32-33 0.36965616154238035 1101 34-35 0.3749127057795576 1101 36-37 0.398206460688435 1101 38-39 0.36665581731009667 1101 40-41 0.3397112014136425 1101 42-43 0.4180876398553508 1101 44-45 0.39973232664751635 1101 46-47 0.37719592523792755 1101 48-49 0.40524991058554605 1101 50-51 0.3843768488475092 1101 52-53 0.30456856287990774 1101 54-55 0.30449820354846224 1101 56-57 0.3062385550403377 1101 58-59 0.42530507056983424 1101 60-61 0.34001117972167805 1101 62-63 0.314342199557089 1101 64-65 0.3502942782427567 1101 66-67 0.22448186822480665 1101 68-69 0.26786160824566707 1101 70-71 0.2575069436355619 1101 72-73 0.21228139580165362 1101 74-75 0.18482791983040414 1101 76-77 0.2043599130040583 1101 78-79 0.3356841915649298 1101 80-81 0.35697629890907834 1101 82-83 0.24369605016897822 1101 84-85 0.24206759779641018 1101 86-87 0.18471132826127246 1101 88-89 0.14244576790900965 1101 90-91 0.1514993654442769 1101 92-93 0.04562102061867179 1101 94-95 0.090371559145904 1101 96-97 0.12017354892628873 1101 98-99 -0.011324706276159446 1101 100 0.11481431409945486 1102 1 0.32984499376121335 1102 2 0.28756694040993835 1102 3 0.35020722274543914 1102 4 0.19487609388125549 1102 5 0.21324544532829748 1102 6 0.1624935465613575 1102 7 0.19916935238980926 1102 8 0.1925739704668601 1102 9 0.2386301834758484 1102 10-11 0.23998677646615363 1102 12-13 0.2788955312912407 1102 14-15 0.3786592756263545 1102 16-17 0.3391224336149392 1102 18-19 0.28407139163756057 1102 20-21 0.330432126465908 1102 22-23 0.28922750878022896 1102 24-25 0.2896056504304383 1102 26-27 0.2643217148377133 1102 28-29 0.35737847977573267 1102 30-31 0.45634047048763193 1102 32-33 0.5266994943530179 1102 34-35 0.5065900137819952 1102 36-37 0.48441989980223354 1102 38-39 0.5115020607558165 1102 40-41 0.5725690644329404 1102 42-43 0.5145468640898798 1102 44-45 0.5081489682365898 1102 46-47 0.49187068140282975 1102 48-49 0.5724330513439426 1102 50-51 0.5576300856218737 1102 52-53 0.5148416403547884 1102 54-55 0.4976353001673317 1102 56-57 0.6003989747809939 1102 58-59 0.5929297003172991 1102 60-61 0.536210268721554 1102 62-63 0.594703366766062 1102 64-65 0.5393556336732814 1102 66-67 0.4937083759878149 1102 68-69 0.37476124538591193 1102 70-71 0.42339528040243124 1102 72-73 0.37612007532148084 1102 74-75 0.39515846252414377 1102 76-77 0.46353758330560524 1102 78-79 0.4465956283589989 1102 80-81 0.4195439966462793 1102 82-83 0.4404679185423106 1102 84-85 0.36397608441120965 1102 86-87 0.2761055661720633 1102 88-89 0.2753098124266238 1102 90-91 0.30040161906439167 1102 92-93 0.24081922886377782 1102 94-95 0.24615703679581102 1102 96-97 0.28597908309751574 1102 98-99 0.2813919300472776 1102 100 0.3074118466280211 1103 1 0.2988413081242811 1103 2 0.27739925311136915 1103 3 0.31497607193337274 1103 4 0.1581990596342706 1103 5 0.18743483275825668 1103 6 0.22707437210831927 1103 7 0.20909786972646316 1103 8 0.21255841728174119 1103 9 0.2971507301111913 1103 10-11 0.26537417797004537 1103 12-13 0.304844129323385 1103 14-15 0.38606990306195854 1103 16-17 0.3563311781332956 1103 18-19 0.3335545558747839 1103 20-21 0.3257331081321837 1103 22-23 0.33871316512733074 1103 24-25 0.35357034415339683 1103 26-27 0.3820807172156435 1103 28-29 0.4303356374310283 1103 30-31 0.4910959065595577 1103 32-33 0.49618758909655014 1103 34-35 0.47045629577980463 1103 36-37 0.4781727005526548 1103 38-39 0.4322332235324211 1103 40-41 0.5351289122700322 1103 42-43 0.514565639597258 1103 44-45 0.5472003057241253 1103 46-47 0.638556498280515 1103 48-49 0.5604816182585779 1103 50-51 0.5708734962111777 1103 52-53 0.5255867712862496 1103 54-55 0.6237333376888756 1103 56-57 0.6227676085925609 1103 58-59 0.6452203835935819 1103 60-61 0.625442621698376 1103 62-63 0.6359912881797314 1103 64-65 0.7474388839826602 1103 66-67 0.7309685833008714 1103 68-69 0.7229759547329593 1103 70-71 0.682988760181658 1103 72-73 0.6442473498405263 1103 74-75 0.5868824995359958 1103 76-77 0.671080723239676 1103 78-79 0.6595098941419408 1103 80-81 0.6945336707221053 1103 82-83 0.6681704731799414 1103 84-85 0.6426616663047149 1103 86-87 0.5887209094247545 1103 88-89 0.6748291400815098 1103 90-91 0.5836138765866359 1103 92-93 0.572168911292767 1103 94-95 0.7266833878212005 1103 96-97 0.7703348996734896 1103 98-99 0.7384141403837212 1103 100 0.8324537642789842 1104 1 0.21948240807491715 1104 2 0.17344102051682597 1104 3 0.22122000395278008 1104 4 0.042842751695211234 1104 5 0.11536893578687568 1104 6 0.152880891335613 1104 7 0.17205488904026112 1104 8 0.15851922005573726 1104 9 0.13772842563857068 1104 10-11 0.16361862536447802 1104 12-13 0.21607646904354283 1104 14-15 0.3101633776920849 1104 16-17 0.2283497224157145 1104 18-19 0.2799345193434206 1104 20-21 0.1921597338560801 1104 22-23 0.2542893298617983 1104 24-25 0.2687382044503792 1104 26-27 0.2838194966006782 1104 28-29 0.3210205515372948 1104 30-31 0.21223606925737215 1104 32-33 0.2654765379750543 1104 34-35 0.42569161722254734 1104 36-37 0.3061664535590509 1104 38-39 0.4386627478163305 1104 40-41 0.46734975702123904 1104 42-43 0.49274432014984626 1104 44-45 0.4387484802863426 1104 46-47 0.44936444446879875 1104 48-49 0.5346512225435234 1104 50-51 0.44191206617598766 1104 52-53 0.42478225106585654 1104 54-55 0.4726630004961976 1104 56-57 0.47946275819639794 1104 58-59 0.5354329110204787 1104 60-61 0.515630683124563 1104 62-63 0.49489844566274854 1104 64-65 0.5934967210746507 1104 66-67 0.6297033211015233 1104 68-69 0.5545777938969394 1104 70-71 0.4551763661059738 1104 72-73 0.5426959919083458 1104 74-75 0.5439785591925741 1104 76-77 0.39821146566599097 1104 78-79 0.5634186027960979 1104 80-81 0.6294801638089815 1104 82-83 0.4556316562989764 1104 84-85 0.5169227858048551 1104 86-87 0.5592795163770106 1104 88-89 0.6089838178013274 1104 90-91 0.4613240107850167 1104 92-93 0.4663586618797808 1104 94-95 0.4803713250857662 1104 96-97 0.4142893424032472 1104 98-99 0.46960480760273526 1104 100 0.6798254632126302 1105 1 0.23132726952663063 1105 2 0.1339538693797806 1105 3 0.16401276245409235 1105 4 0.11716892122782241 1105 5 0.10244213319106166 1105 6 0.10746292643020183 1105 7 0.11578533993617413 1105 8 0.09234076365934385 1105 9 0.12746909206477142 1105 10-11 0.18502944459466875 1105 12-13 0.20941059083600777 1105 14-15 0.2657239630731425 1105 16-17 0.29697429019812915 1105 18-19 0.31181938376771967 1105 20-21 0.2613837269393926 1105 22-23 0.2708223511949086 1105 24-25 0.2916868210822159 1105 26-27 0.2821997568465733 1105 28-29 0.3870518893137884 1105 30-31 0.3537847238319145 1105 32-33 0.4188566864470502 1105 34-35 0.4112531481503865 1105 36-37 0.43363295612955 1105 38-39 0.44445535466764596 1105 40-41 0.44165603382054286 1105 42-43 0.44537212454189756 1105 44-45 0.3685484291724421 1105 46-47 0.4625182689185223 1105 48-49 0.45271385459532354 1105 50-51 0.4981596381145721 1105 52-53 0.5593975253016126 1105 54-55 0.48013976814001325 1105 56-57 0.4421409998056518 1105 58-59 0.4897122503098572 1105 60-61 0.5592185014611672 1105 62-63 0.5576501257453046 1105 64-65 0.45887653785004545 1105 66-67 0.43390603485098467 1105 68-69 0.5206935360726135 1105 70-71 0.5384135587806114 1105 72-73 0.5247959304629077 1105 74-75 0.6229100399792387 1105 76-77 0.5581799303215682 1105 78-79 0.6631488651868338 1105 80-81 0.6022852718157985 1105 82-83 0.6152930448208167 1105 84-85 0.530509129934341 1105 86-87 0.5893317419766149 1105 88-89 0.6177343547691869 1105 90-91 0.6198934891392405 1105 92-93 0.658417941922945 1105 94-95 0.7592879127554717 1105 96-97 0.7637047498009899 1105 98-99 0.7405846931341777 1105 100 0.8286524112353497 1106 1 0.1921358725058795 1106 2 0.1676761568871683 1106 3 0.14984497260991958 1106 4 0.14131579861258814 1106 5 0.12586723838197855 1106 6 0.09271059956691374 1106 7 0.13031909024631716 1106 8 0.09925720440298136 1106 9 0.0908297239888256 1106 10-11 0.09606053214858434 1106 12-13 0.1231075398223922 1106 14-15 0.224287271355756 1106 16-17 0.23481546577948365 1106 18-19 0.2187803729273483 1106 20-21 0.2488623047709737 1106 22-23 0.22233205929599364 1106 24-25 0.22274311083080534 1106 26-27 0.19733653911493576 1106 28-29 0.18129629758631438 1106 30-31 0.24295785312411056 1106 32-33 0.29241961756013524 1106 34-35 0.2966114879743671 1106 36-37 0.24567650906036675 1106 38-39 0.26706480426371115 1106 40-41 0.29590674945804807 1106 42-43 0.24911514576972138 1106 44-45 0.35811196678623247 1106 46-47 0.2697480633111482 1106 48-49 0.34969143921382795 1106 50-51 0.3396994379814515 1106 52-53 0.41065951330913464 1106 54-55 0.3277069338519425 1106 56-57 0.32399416240994583 1106 58-59 0.44411715102944527 1106 60-61 0.5228406527331018 1106 62-63 0.5021077484018903 1106 64-65 0.49885817317831993 1106 66-67 0.5014116537679811 1106 68-69 0.43824776290084344 1106 70-71 0.4526664919931349 1106 72-73 0.45266528924140204 1106 74-75 0.43256757914353017 1106 76-77 0.4289365838797039 1106 78-79 0.5501928043755981 1106 80-81 0.6043600828599303 1106 82-83 0.5545490605231862 1106 84-85 0.49977912968895666 1106 86-87 0.4269735175755969 1106 88-89 0.5293089299687601 1106 90-91 0.5462487167845289 1106 92-93 0.5943464590590146 1106 94-95 0.5980055303286029 1106 96-97 0.6568224057878531 1106 98-99 0.7291528213100129 1106 100 0.8938389677624556 1107 1 0.1901065046153363 1107 2 0.16783722415620161 1107 3 0.22357271179082971 1107 4 0.0990311449535426 1107 5 0.10018922537674513 1107 6 0.08814607221329851 1107 7 0.13524323570687358 1107 8 0.12945172594402976 1107 9 0.1461712122523835 1107 10-11 0.1315812197661046 1107 12-13 0.1813715883277709 1107 14-15 0.23255080430640618 1107 16-17 0.20017118385182897 1107 18-19 0.13543738850901121 1107 20-21 0.1980394545084465 1107 22-23 0.2980726785783503 1107 24-25 0.30714178696594985 1107 26-27 0.3777498501304777 1107 28-29 0.3786393800347767 1107 30-31 0.22930038480071602 1107 32-33 0.21822842705061163 1107 34-35 0.26724722125732825 1107 36-37 0.37649918298823337 1107 38-39 0.41381118316416377 1107 40-41 0.3867287892785001 1107 42-43 0.37059904379987074 1107 44-45 0.4160188504282232 1107 46-47 0.40898822678833113 1107 48-49 0.45119544935795375 1107 50-51 0.44519731616239255 1107 52-53 0.4300265635236258 1107 54-55 0.4403557180916877 1107 56-57 0.5545764782449538 1107 58-59 0.5399767209930388 1107 60-61 0.5605177414259046 1107 62-63 0.4846851489828907 1107 64-65 0.6166397406569857 1107 66-67 0.6886590412877638 1107 68-69 0.579190215884374 1107 70-71 0.7364160996609641 1107 72-73 0.7011298202960461 1107 74-75 0.7364142077368498 1107 76-77 0.6731895059667323 1107 78-79 0.6605555765816504 1107 80-81 0.5518660133582749 1107 82-83 0.5869694460455577 1107 84-85 0.5955451775255618 1107 86-87 0.5640830826202468 1107 88-89 0.5532526492244507 1107 90-91 0.5314073031643254 1107 92-93 0.5161197576374832 1107 94-95 0.7374199986275407 1107 96-97 0.7768284176043672 1107 98-99 0.766618295954359 1107 100 0.9123043700298936 1108 1 0.22650670291609742 1108 2 0.1655767089649558 1108 3 0.2505388582787589 1108 4 0.15641200491209162 1108 5 0.11866096854762986 1108 6 0.10282145365373196 1108 7 0.11051138796855042 1108 8 0.1273777894514012 1108 9 0.06659047543075047 1108 10-11 0.14753476573821445 1108 12-13 0.1670997743524083 1108 14-15 0.16194786783391635 1108 16-17 0.19244254421244733 1108 18-19 0.2282036645624146 1108 20-21 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-0.24708994545151697 2316 86-87 -0.1579291387768329 2316 88-89 -0.17789249820326347 2316 90-91 -0.14739822151163295 2316 92-93 -0.039723786182893406 2316 94-95 0.017940284013317154 2316 96-97 0.00946992728164986 2316 98-99 -0.14561462071479525 2316 100 -0.2974995082422929 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 4.0 10 5.0 11 21.0 12 40.0 13 204.0 14 540.0 15 1609.0 16 3560.0 17 7291.0 18 13389.0 19 20124.0 20 28321.0 21 37851.0 22 48936.0 23 55831.0 24 59000.0 25 63468.0 26 72493.0 27 86634.0 28 104814.0 29 126615.0 30 152190.0 31 183892.0 32 226055.0 33 283988.0 34 374827.0 35 512255.0 36 745946.0 37 1169778.0 38 1209722.0 39 201660.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20246825063379 20.2681170794952 13.8740538392717 23.655360830599303 2 15.043986293487333 21.507439913046934 40.237617568942966 23.210956224522768 3 17.11227647199913 27.2040335324163 31.141141343379175 24.542548652205394 4 11.78243101505324 17.840144941000297 38.74555650222775 31.631867541718716 5 12.136690679782186 38.39181889420863 35.911160106662024 13.560330319347152 6 29.947712418300654 38.46406092159453 18.320114659690383 13.268112000414433 7 26.151678102907344 33.21072550553065 23.277920074845976 17.359676316716033 8 25.150875135359808 36.55907126984118 20.792734025470697 17.497319569328308 9 26.158741473322202 15.373447032155783 20.89140747103006 37.57640402349195 10-11 24.093871398715233 26.71470653294637 29.55548287689388 19.635939191444514 12-13 24.728388924163365 24.693283288664162 29.34080837589726 21.237519411275212 14-15 22.10204533064829 26.06906848154571 27.038540047621407 24.790346140184592 16-17 21.511990184162645 28.810960776626064 27.791801814161666 21.88524722504962 18-19 21.312482337975833 27.88735295892706 29.796202797552386 21.003961905544724 20-21 22.153208895216046 27.18654519008712 29.79682555964213 20.8634203550547 22-23 21.890884846781084 27.098528723334265 29.534882275245796 21.475704154638855 24-25 21.617190538745497 27.245040551502157 29.695191365014463 21.44257754473789 26-27 21.598243644279254 27.300968972637584 29.661434598922966 21.439352784160196 28-29 21.5527023894271 27.374513957571466 29.78420514821839 21.288578504783043 30-31 21.922830187213076 27.28357562042447 29.689992825067197 21.10360136729526 32-33 21.666621624970286 27.36072380768514 29.643923372936353 21.32873119440822 34-35 21.88820775887272 27.481489386649706 29.570347583531102 21.059955270946475 36-37 21.908531697122573 27.39180343367687 29.439343397474282 21.26032147172628 38-39 21.752568080222428 27.592648522301594 29.43094101725799 21.223842380217988 40-41 21.89136839065955 27.36232171370701 29.493153051702865 21.253156843930576 42-43 21.70052696519681 27.48976160933772 29.284439459444055 21.525271966021407 44-45 21.71315707674242 27.707864198383643 29.166781791807693 21.412196933066244 46-47 21.7113798270764 27.542506679883243 28.998978104143582 21.74713538889678 48-49 21.716110256465164 27.667033584937723 29.00201396338738 21.61484219520973 50-51 21.766850325915296 27.846161418087718 28.809824172389288 21.5771640836077 52-53 21.79950209541108 27.919637615670258 28.606218566351487 21.674641722567177 54-55 21.760049843195283 27.840042438552082 28.73246771995184 21.6674399983008 56-57 21.790518838405397 27.986218462783906 28.46905923325694 21.754203465553758 58-59 21.772776974600806 27.854275299901982 28.685977911520368 21.686969813976845 60-61 21.962726247567208 27.98701186563904 28.615013461309314 21.435248425484442 62-63 21.75750152669435 27.962331061943722 28.723545357329378 21.556622054032545 64-65 21.715596350870793 27.751961236852345 28.664528469946006 21.867913942330855 66-67 21.62573925832005 27.824754492971394 28.558894530392926 21.990611718315627 68-69 21.603677345138212 27.8768569831339 28.605920551232188 21.91354512049569 70-71 21.637812571638758 27.745987258176697 28.59476865996721 22.021431510217333 72-73 21.586538125287692 27.954978224001753 28.378098921116912 22.080384729593643 74-75 21.598964237886825 28.01353156371931 28.27983708936686 22.107667109027 76-77 21.47548288705888 27.887999575888102 28.442496530562604 22.194021006490413 78-79 21.497755127045984 27.82099455509189 28.415194902048384 22.266055415813746 80-81 21.467161951282776 28.04720532912478 28.161663841324717 22.323968878267724 82-83 21.566662651221453 28.02772125211081 28.24634281453349 22.159273282134247 84-85 21.51084183139262 28.038833204247975 28.146137115331328 22.30418784902808 86-87 21.36099392133068 28.01850475857869 28.285545465316837 22.334955854773796 88-89 21.331504268034926 27.89664831124508 28.290605487109538 22.48124193361046 90-91 21.51057581317632 27.89418331570712 28.226101888655887 22.36913898246067 92-93 21.363985610163677 27.902116154432672 28.207725710068914 22.526172525334744 94-95 21.234295583424615 27.800071412567885 28.24780782936715 22.71782517464035 96-97 21.26215374751847 27.78033976432257 28.234156819138832 22.723349669020124 98-99 21.149196098995734 27.681672732391227 28.196219461066597 22.97291170754644 100 20.296478909241138 28.121541465113097 28.461971832931955 23.12000779271381 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2380.0 1 1726.5 2 2123.0 3 3101.0 4 2275.5 5 1765.0 6 2647.0 7 4970.5 8 7814.5 9 9144.5 10 7857.5 11 6224.5 12 6343.0 13 6816.0 14 7177.0 15 7670.5 16 8185.5 17 8727.0 18 9461.0 19 10352.5 20 11577.0 21 13343.0 22 15907.0 23 19572.5 24 24234.5 25 30323.5 26 38145.0 27 46890.0 28 56344.5 29 67454.5 30 79965.0 31 91902.0 32 104206.0 33 118740.5 34 133659.5 35 146103.5 36 161163.0 37 175429.5 38 184446.5 39 192909.0 40 196936.0 41 198383.0 42 200747.5 43 203664.5 44 206516.0 45 210540.0 46 213108.5 47 212765.0 48 213188.5 49 212053.0 50 209823.5 51 201976.0 52 189151.0 53 178855.0 54 168753.5 55 154223.0 56 137368.0 57 121526.5 58 106191.0 59 90976.5 60 75313.5 61 60760.5 62 48806.5 63 37881.5 64 28274.0 65 20927.5 66 15439.0 67 11777.0 68 9014.0 69 6811.5 70 5315.0 71 4167.5 72 3152.5 73 2447.5 74 1862.0 75 1386.0 76 1028.5 77 681.0 78 431.5 79 284.5 80 192.5 81 134.5 82 100.0 83 77.5 84 53.5 85 38.5 86 26.5 87 17.5 88 12.0 89 8.0 90 6.5 91 6.5 92 4.5 93 3.0 94 1.5 95 3.0 96 5.0 97 3.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032809005139444315 2 0.0701594673060854 3 6.907158976725119E-4 4 8.115911797652016E-4 5 0.0015713786672049645 6 7.425195899979503E-4 7 0.0 8 7.943232823233887E-4 9 6.907158976725119E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.511535387188567E-4 18-19 0.001148315179880551 20-21 0.003151391283130835 22-23 0.0 24-25 8.633948720906399E-6 26-27 1.8994687185994077E-4 28-29 0.0010447077952296742 30-31 0.0016922539492976543 32-33 0.0028060333342945793 34-35 0.0039025448218496924 36-37 0.0012173867696478023 38-39 0.0016231823595304032 40-41 0.005206271078706558 42-43 0.006699944207423365 44-45 0.00505949395045115 46-47 0.006656774463818834 48-49 0.008996574567184468 50-51 0.008254054977186518 52-53 0.0038766429756869735 54-55 0.0022793624623192892 56-57 0.0027110598983646093 58-59 0.003470847385804373 60-61 0.007304320617886814 62-63 0.013900657440659301 64-65 0.004006152206500569 66-67 0.009954942875205078 68-69 0.012432886158105215 70-71 0.005534361130101002 72-73 0.007399294053816783 74-75 0.0032463647190608064 76-77 0.002909640718945457 78-79 0.007830991489862105 80-81 0.00601786225847176 82-83 0.016413136518443065 84-85 0.01720745980076645 86-87 0.023121714674587335 88-89 0.021593505750986904 90-91 0.024520414367374174 92-93 0.03465667016571829 94-95 0.0379203027822209 96-97 0.02270728513598383 98-99 0.01805358677541528 100 0.018459382365297882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5791093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.14389331574976 #Duplication Level Percentage of deduplicated Percentage of total 1 76.01162749295145 48.756817246643905 2 15.65437289547256 20.08264849864313 3 4.428911398935217 8.522628608346258 4 1.6036528018846565 4.114581369583704 5 0.752987739805052 2.41497826250614 6 0.39877614347429735 1.5347432642328873 7 0.2497922352924649 1.1215852544191771 8 0.1722096191798906 0.883695635249664 9 0.12080326647076817 0.697391265302554 >10 0.5573344188624998 6.14461883344974 >50 0.030724604765472643 1.3585246087211713 >100 0.017047775524296482 2.029250413265671 >500 0.0012420761302104053 0.5345299398138599 >1k 4.140250008814964E-4 0.5494679608171781 >5k 0.0 0.0 >10k+ 1.0350625022018456E-4 1.2545388390049215 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22108 0.3817586766435973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20681 0.3571173869941305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13898 0.23998923864631425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.7267897441812798E-5 5 0.0 0.0 0.0 0.0 1.7267897441812798E-5 6 6.907158976725119E-5 0.0 0.0 0.0 3.4535794883625596E-5 7 6.907158976725119E-5 0.0 0.0 0.0 3.4535794883625596E-5 8 6.907158976725119E-5 0.0 0.0 0.0 3.4535794883625596E-5 9 6.907158976725119E-5 0.0 0.0 0.0 3.4535794883625596E-5 10-11 6.907158976725119E-5 0.0 0.0 0.0 3.4535794883625596E-5 12-13 6.907158976725119E-5 0.0 0.0 8.633948720906399E-6 3.4535794883625596E-5 14-15 6.907158976725119E-5 0.0 0.0 1.7267897441812798E-5 8.633948720906399E-5 16-17 1.7267897441812798E-4 0.0 0.0 1.7267897441812798E-5 8.633948720906399E-5 18-19 1.7267897441812798E-4 0.0 0.0 1.7267897441812798E-5 1.2087528209268958E-4 20-21 1.7267897441812798E-4 0.0 0.0 1.7267897441812798E-5 1.2087528209268958E-4 22-23 1.8131292313903439E-4 0.0 0.0 3.4535794883625596E-5 1.3814317953450239E-4 24-25 1.8994687185994077E-4 0.0 0.0 1.3814317953450239E-4 1.7267897441812798E-4 26-27 1.8994687185994077E-4 0.0 0.0 3.02188205231724E-4 1.7267897441812798E-4 28-29 1.8994687185994077E-4 0.0 0.0 0.0011569491286014574 1.7267897441812798E-4 30-31 1.8994687185994077E-4 0.0 0.0 0.004645064411847643 1.7267897441812798E-4 32-33 1.8994687185994077E-4 0.0 0.0 0.012605565132523342 1.8994687185994077E-4 34-35 1.8994687185994077E-4 0.0 0.0 0.024667191495629584 1.8994687185994077E-4 36-37 1.8994687185994077E-4 0.0 0.0 0.04502604257952687 1.9858082058084715E-4 38-39 1.8994687185994077E-4 0.0 0.0 0.07639317828257983 2.0721476930175357E-4 40-41 1.8994687185994077E-4 8.633948720906399E-6 0.0 0.11332921091061739 2.0721476930175357E-4 42-43 1.8994687185994077E-4 1.7267897441812798E-5 0.0 0.15000622507702777 2.3311661546447274E-4 44-45 1.8994687185994077E-4 1.7267897441812798E-5 0.0 0.19412570304085947 2.5038451290628557E-4 46-47 1.8994687185994077E-4 1.7267897441812798E-5 0.0 0.2509198177269818 2.5901846162719195E-4 48-49 1.8994687185994077E-4 1.7267897441812798E-5 0.0 0.3135073120048323 2.5901846162719195E-4 50-51 1.9858082058084715E-4 1.7267897441812798E-5 0.0 0.3802304677199969 2.7628635906900477E-4 52-53 2.0721476930175357E-4 1.7267897441812798E-5 0.0 0.4489480655896909 2.8492030778991116E-4 54-55 2.0721476930175357E-4 1.7267897441812798E-5 0.0 0.5297963614122585 3.02188205231724E-4 56-57 2.0721476930175357E-4 1.7267897441812798E-5 0.0 0.6255986564194358 3.1082215395263036E-4 58-59 2.0721476930175357E-4 1.7267897441812798E-5 0.0 0.735172099636459 3.1945610267353675E-4 60-61 2.1584871802265998E-4 1.7267897441812798E-5 0.0 0.8514023173173009 3.6262584627806877E-4 62-63 2.2448266674356636E-4 1.7267897441812798E-5 0.0 0.9706630509991809 3.6262584627806877E-4 64-65 2.2448266674356636E-4 1.7267897441812798E-5 0.0 1.0952595649905812 3.6262584627806877E-4 66-67 2.5901846162719195E-4 4.3169743604531994E-5 0.0 1.244721851298192 3.6262584627806877E-4 68-69 2.5901846162719195E-4 5.180369232543839E-5 0.0 1.4181865150499222 3.6262584627806877E-4 70-71 2.762863590690047E-4 5.180369232543839E-5 0.0 1.6072527241403307 3.6262584627806877E-4 72-73 3.1082215395263036E-4 5.180369232543839E-5 0.0 1.7950670106662074 3.885276924407879E-4 74-75 3.1082215395263036E-4 5.180369232543839E-5 0.0 1.9910144768871785 3.9716164116169436E-4 76-77 3.280900513944432E-4 5.180369232543839E-5 0.0 2.2062242827044916 3.9716164116169436E-4 78-79 3.280900513944432E-4 5.180369232543839E-5 0.0 2.4558835439182207 3.9716164116169436E-4 80-81 3.280900513944432E-4 5.180369232543839E-5 0.0 2.7253922532413135 4.3169743604531995E-4 82-83 3.280900513944432E-4 5.180369232543839E-5 0.0 3.008775718158904 4.6623323092894554E-4 84-85 3.280900513944432E-4 5.180369232543839E-5 0.0 3.2981425095400816 4.6623323092894554E-4 86-87 3.280900513944432E-4 5.180369232543839E-5 0.0 3.604190089850051 4.6623323092894554E-4 88 3.4535794883625595E-4 5.180369232543839E-5 0.0 3.8499122704470468 4.6623323092894554E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10700 0.0 50.985653 1 GTATCAA 18170 0.0 37.142467 1 TACCTGG 5275 0.0 31.739315 2 TCAACGC 22530 0.0 29.304632 4 ATCAACG 22660 0.0 29.136763 3 CAACGCA 22885 0.0 28.870583 5 GTACCTG 5840 0.0 28.829954 1 TATCAAC 23610 0.0 28.145967 2 AACGCAG 23750 0.0 27.977371 6 ACCTGGG 6080 0.0 26.278479 3 GTACATG 19940 0.0 25.23669 1 ACGCAGA 26765 0.0 24.72046 7 TACATGG 20550 0.0 24.029652 2 CGCAGAG 27445 0.0 23.98811 8 ACATGGG 19905 0.0 23.560974 3 CATGGGG 11185 0.0 21.888847 4 TAACGCA 565 0.0 21.624498 4 GCAGAGT 30145 0.0 21.402718 9 GAGTACT 19210 0.0 20.658068 12-13 AGAGTAC 27830 0.0 20.253527 10-11 >>END_MODULE