FastQCFastQC Report
Mon 6 Jun 2016
ERR1211480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6265390
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230270.36752700151147816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209420.3342489453968548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.20527054181782778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110500.052.1962781
GTATCAA191950.037.7744671
TCAACGC229700.030.8308144
CAACGCA231950.030.5095375
ATCAACG232950.030.4021363
AACGCAG241100.029.518596
TATCAAC243450.029.3789732
TACCTGG53000.029.3676572
GTACCTG59500.027.0629331
GTACATG212800.026.9710461
ACGCAGA270650.026.0365987
TACATGG216800.025.854382
CGCAGAG275800.025.3814988
ACATGGG217600.024.6638433
CATGGGG131450.023.9880354
ACCTGGG68650.022.2482783
GCAGAGT305900.022.2259039
ATGGGGG87250.022.1889515
GAGTACT189400.021.60821512-13
CAGAGTA298850.020.40734910-11
Produced by FastQC (version 0.11.5)