FastQCFastQC Report
Mon 6 Jun 2016
ERR1211480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6265390
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249410.39807577820375106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235490.3758584860639162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158260.25259401250361113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123900.049.456781
GTATCAA202200.035.259061
TACCTGG54050.029.9301242
TCAACGC254100.027.5933764
ATCAACG255950.027.3392833
CAACGCA257950.027.1266635
TATCAAC263700.026.6610262
AACGCAG265400.026.5239186
GTACCTG63850.026.4415111
GTACATG235250.025.4878541
TACATGG239650.024.2934382
ACATGGG232350.023.8461763
CATGGGG137650.023.7956314
ACCTGGG68200.023.2216533
ACGCAGA304250.022.8899277
CGCAGAG309950.022.4234988
ATGGGGG88800.021.7503415
GAGTACT211400.020.26262912-13
GCAGAGT340700.019.9717589
AGAGTAC320300.019.109510-11
Produced by FastQC (version 0.11.5)