##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6265390 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13829641889811 33.0 31.0 34.0 30.0 34.0 2 32.35362794654443 34.0 31.0 34.0 31.0 34.0 3 32.36352533521457 34.0 31.0 34.0 30.0 34.0 4 35.99721549656127 37.0 35.0 37.0 35.0 37.0 5 35.92103556203205 37.0 35.0 37.0 35.0 37.0 6 35.93919436778876 37.0 35.0 37.0 35.0 37.0 7 35.86786872006372 37.0 35.0 37.0 35.0 37.0 8 35.86270623217389 37.0 35.0 37.0 35.0 37.0 9 37.518167743747796 39.0 37.0 39.0 35.0 39.0 10-11 37.4821222621417 39.0 37.0 39.0 35.0 39.0 12-13 37.39391889411513 39.0 37.0 39.0 34.0 39.0 14-15 38.63268886693406 40.0 38.0 41.0 34.0 41.0 16-17 38.55998908288231 40.0 38.0 41.0 34.0 41.0 18-19 38.5519404219051 40.0 38.0 41.0 34.0 41.0 20-21 38.49624460727904 40.0 38.0 41.0 34.0 41.0 22-23 38.38994308414959 40.0 38.0 41.0 34.0 41.0 24-25 38.277623420090364 40.0 38.0 41.0 34.0 41.0 26-27 38.10230751796776 40.0 38.0 41.0 33.0 41.0 28-29 38.13708077230628 40.0 38.0 41.0 33.0 41.0 30-31 38.05419040155521 40.0 37.5 41.0 33.0 41.0 32-33 37.864505481701855 40.0 37.0 41.0 33.0 41.0 34-35 37.743566641501964 40.0 37.0 41.0 33.0 41.0 36-37 37.68364036396777 40.0 37.0 41.0 33.0 41.0 38-39 37.585367949960016 40.0 37.0 41.0 32.5 41.0 40-41 37.42274894300275 40.0 36.5 41.0 31.5 41.0 42-43 37.267869518098635 40.0 36.0 41.0 31.0 41.0 44-45 37.079201454338836 39.5 36.0 41.0 31.0 41.0 46-47 36.81764614812486 39.0 35.5 41.0 30.0 41.0 48-49 36.58815748101874 39.0 35.0 41.0 30.0 41.0 50-51 36.38543721939097 39.0 35.0 41.0 29.0 41.0 52-53 36.18473566689384 39.0 35.0 40.0 28.5 41.0 54-55 35.99089178806108 39.0 35.0 40.0 28.0 41.0 56-57 35.79272998169308 38.0 34.5 40.0 28.0 41.0 58-59 35.53506126514071 38.0 34.0 40.0 27.0 41.0 60-61 35.22101233921592 38.0 34.0 40.0 26.0 41.0 62-63 34.89292781454945 37.0 34.0 40.0 26.0 41.0 64-65 34.581595559095284 37.0 33.0 40.0 26.0 41.0 66-67 34.24068038861108 36.0 33.0 39.0 25.5 41.0 68-69 33.841913033346685 36.0 32.5 39.0 25.0 40.0 70-71 33.45538434159725 35.0 32.0 38.5 24.0 40.0 72-73 33.04752497450278 35.0 31.5 38.0 23.5 40.0 74-75 32.642379644363714 35.0 31.0 37.0 22.5 39.0 76-77 31.091835384549086 33.5 29.5 35.5 21.0 38.5 78-79 31.922293185260614 34.0 31.0 36.0 22.0 39.0 80-81 31.894557082639707 34.0 31.0 36.0 22.0 37.5 82-83 31.607468329984247 34.0 31.0 35.5 21.5 37.0 84-85 31.306380847800376 34.0 30.5 35.0 20.0 37.0 86-87 31.007466414700442 34.0 30.0 35.0 20.0 36.0 88-89 30.73121425801107 34.0 30.0 35.0 20.0 36.0 90-91 30.44741149393733 34.0 29.5 35.0 19.0 35.5 92-93 30.23331476572089 34.0 29.0 35.0 18.5 35.0 94-95 30.011662881321037 34.0 29.0 35.0 18.0 35.0 96-97 29.808426291100794 34.0 29.0 35.0 18.0 35.0 98-99 29.643760404380252 34.0 29.0 35.0 17.5 35.0 100 27.774930371453333 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3121289725853984 1101 2 0.2126621380238234 1101 3 0.2553732922067127 1101 4 0.1502723433339881 1101 5 0.14944509656407945 1101 6 0.19408964137774376 1101 7 0.20832171123795717 1101 8 0.18915544465502876 1101 9 0.17677767898624097 1101 10-11 0.21113581469637666 1101 12-13 0.2231720875283969 1101 14-15 0.2858682793317371 1101 16-17 0.2455705397756205 1101 18-19 0.2898761099831688 1101 20-21 0.26835106999448044 1101 22-23 0.24549358541795385 1101 24-25 0.19582151151049487 1101 26-27 0.20751791326367908 1101 28-29 0.25399686401276966 1101 30-31 0.2531761390591356 1101 32-33 0.3095290011789089 1101 34-35 0.3033123123780257 1101 36-37 0.32322962522646037 1101 38-39 0.25283438846447837 1101 40-41 0.28353810075504526 1101 42-43 0.30371788443199677 1101 44-45 0.2987055995926866 1101 46-47 0.35768022986783166 1101 48-49 0.3204077168798065 1101 50-51 0.3248069174821353 1101 52-53 0.2529166909286644 1101 54-55 0.27468761840655276 1101 56-57 0.3033341269272327 1101 58-59 0.20711662940116327 1101 60-61 0.1643156487023134 1101 62-63 0.23719250553265425 1101 64-65 0.17912819196180152 1101 66-67 0.11819318731908623 1101 68-69 0.12721802240463376 1101 70-71 0.1441841149671319 1101 72-73 0.05567631730217215 1101 74-75 0.04268773633425127 1101 76-77 0.055205389403944594 1101 78-79 0.2556302200705254 1101 80-81 0.22534052300339624 1101 82-83 0.05340192340331029 1101 84-85 0.10458397402651443 1101 86-87 0.1158189342584457 1101 88-89 0.06662845975845144 1101 90-91 0.022631626048845277 1101 92-93 -0.05887510558828879 1101 94-95 -0.01711904991691071 1101 96-97 0.014474130983458622 1101 98-99 -0.07346399051719743 1101 100 -0.07482740261813348 1102 1 0.2996651556065828 1102 2 0.24363005681773586 1102 3 0.3231818263176933 1102 4 0.22298874146014214 1102 5 0.20154168567746922 1102 6 0.21331678059250692 1102 7 0.21660912529797116 1102 8 0.2319141497165873 1102 9 0.2713711177091511 1102 10-11 0.26695418455453535 1102 12-13 0.28156960177582135 1102 14-15 0.35133960833260147 1102 16-17 0.34173791224440464 1102 18-19 0.35667393436268213 1102 20-21 0.3565530236948362 1102 22-23 0.2890203400775846 1102 24-25 0.3194137423906369 1102 26-27 0.33604917995595684 1102 28-29 0.36276203966795606 1102 30-31 0.42365819797537796 1102 32-33 0.5250856947687907 1102 34-35 0.4365914443377079 1102 36-37 0.441297064436057 1102 38-39 0.4311328140879169 1102 40-41 0.4336561612328609 1102 42-43 0.413332741279099 1102 44-45 0.5255315928963569 1102 46-47 0.515925592380789 1102 48-49 0.4752611306549852 1102 50-51 0.4620842468942996 1102 52-53 0.4436901582612549 1102 54-55 0.45993952761475754 1102 56-57 0.5220610035965976 1102 58-59 0.5122436331056335 1102 60-61 0.511634982291099 1102 62-63 0.5226717000830732 1102 64-65 0.4509349132273357 1102 66-67 0.5064747439234978 1102 68-69 0.47697339021999596 1102 70-71 0.455196811166914 1102 72-73 0.40294868208819423 1102 74-75 0.31850580549320995 1102 76-77 0.39610592789236065 1102 78-79 0.33934863545578153 1102 80-81 0.34505265295158694 1102 82-83 0.3190674093450596 1102 84-85 0.29997674742155667 1102 86-87 0.26636461577000503 1102 88-89 0.2787240643909108 1102 90-91 0.27560719393009236 1102 92-93 0.15784638547605212 1102 94-95 0.25802539125165325 1102 96-97 0.2742065128166544 1102 98-99 0.23853283648838897 1102 100 0.40954564109931013 1103 1 0.3182689097654432 1103 2 0.25142679508616084 1103 3 0.32868119346819924 1103 4 0.15623374769723597 1103 5 0.1918736871646658 1103 6 0.20475201093744033 1103 7 0.197998201248339 1103 8 0.24079445549922696 1103 9 0.22721080585749576 1103 10-11 0.22902068925208852 1103 12-13 0.24002867647602955 1103 14-15 0.37559526754465367 1103 16-17 0.371081776854119 1103 18-19 0.3804703955849007 1103 20-21 0.36113013571767993 1103 22-23 0.39365007869241 1103 24-25 0.3350021004785333 1103 26-27 0.3972833882969411 1103 28-29 0.42428043363679535 1103 30-31 0.47088488727425215 1103 32-33 0.49910542293650195 1103 34-35 0.5387264468811921 1103 36-37 0.5977597917182891 1103 38-39 0.6058270400814507 1103 40-41 0.6051375246454782 1103 42-43 0.6168339126828499 1103 44-45 0.6391055874385074 1103 46-47 0.6446777175780483 1103 48-49 0.6421664280769761 1103 50-51 0.6833368897759513 1103 52-53 0.6585273230345834 1103 54-55 0.6878535778891006 1103 56-57 0.7040946385087992 1103 58-59 0.7427749372447892 1103 60-61 0.691055242797816 1103 62-63 0.7635815226607789 1103 64-65 0.8148510567847964 1103 66-67 0.7676277273546503 1103 68-69 0.6893688720513751 1103 70-71 0.6893198548200772 1103 72-73 0.7103306533470928 1103 74-75 0.7672551622595662 1103 76-77 0.7970331085115561 1103 78-79 0.8157417701127265 1103 80-81 0.7637654280415518 1103 82-83 0.7162056987655205 1103 84-85 0.6499975715872957 1103 86-87 0.6339678807310882 1103 88-89 0.6837768874947763 1103 90-91 0.7473252449831698 1103 92-93 0.7934611827900433 1103 94-95 0.7473287266828059 1103 96-97 0.7863115429824994 1103 98-99 0.7843496876206402 1103 100 0.9116375125860259 1104 1 0.19292214098892657 1104 2 0.1956813381916831 1104 3 0.2588641267780858 1104 4 0.07913067801638363 1104 5 0.1520406756680046 1104 6 0.16075605454800268 1104 7 0.1571372156719235 1104 8 0.14077788957407478 1104 9 0.228539726196324 1104 10-11 0.2141377303308971 1104 12-13 0.23403818642754004 1104 14-15 0.3180828545991403 1104 16-17 0.25616344027686466 1104 18-19 0.30607801760104536 1104 20-21 0.16217653410613053 1104 22-23 0.2298628430153613 1104 24-25 0.24233273782288478 1104 26-27 0.39634551577857025 1104 28-29 0.38543409535472506 1104 30-31 0.3252729431179091 1104 32-33 0.37731695107182617 1104 34-35 0.44512134563699135 1104 36-37 0.3126773016567057 1104 38-39 0.39052975278691804 1104 40-41 0.487021338837458 1104 42-43 0.4494903689994203 1104 44-45 0.366593705074969 1104 46-47 0.43698266924412366 1104 48-49 0.45797987984852284 1104 50-51 0.4061494532111567 1104 52-53 0.49584309082440114 1104 54-55 0.5199875114464803 1104 56-57 0.6000018522565185 1104 58-59 0.5011684083808134 1104 60-61 0.48153831561698723 1104 62-63 0.5089640092790617 1104 64-65 0.6029480334951742 1104 66-67 0.5490775955212897 1104 68-69 0.5536818769614484 1104 70-71 0.36688691128597384 1104 72-73 0.4620904514102051 1104 74-75 0.526568253562715 1104 76-77 0.4379269441766702 1104 78-79 0.5681444818693215 1104 80-81 0.6454157219417027 1104 82-83 0.471594617421232 1104 84-85 0.6429894041884161 1104 86-87 0.6109133586582338 1104 88-89 0.6599802861447301 1104 90-91 0.47148606879112975 1104 92-93 0.4745048563780081 1104 94-95 0.56898300336616 1104 96-97 0.550814486293266 1104 98-99 0.460741238799919 1104 100 0.6563815916247968 1105 1 0.19547463342957627 1105 2 0.11156453663053867 1105 3 0.1646495570418196 1105 4 0.08565290009242688 1105 5 0.055453635629980624 1105 6 0.08726284900984638 1105 7 0.08868292305984227 1105 8 0.12051311714220958 1105 9 0.08486515626354674 1105 10-11 0.08469631687899692 1105 12-13 0.13307243260024393 1105 14-15 0.20305428670107517 1105 16-17 0.23531682246986207 1105 18-19 0.24505627872040492 1105 20-21 0.17789576834757526 1105 22-23 0.2646264336460149 1105 24-25 0.24904268418761433 1105 26-27 0.21839977238622765 1105 28-29 0.28545838002484913 1105 30-31 0.3329256326170267 1105 32-33 0.35127864715681767 1105 34-35 0.3867612407412082 1105 36-37 0.4053056656851197 1105 38-39 0.3702856096265208 1105 40-41 0.4637573492770741 1105 42-43 0.41721696666260044 1105 44-45 0.4215603468560616 1105 46-47 0.4294307738241656 1105 48-49 0.46208293994178007 1105 50-51 0.48513225152124306 1105 52-53 0.49368471947020254 1105 54-55 0.43821872065735334 1105 56-57 0.4821002159535297 1105 58-59 0.49607331139451816 1105 60-61 0.44968012160374826 1105 62-63 0.47731695825319775 1105 64-65 0.5100271126744431 1105 66-67 0.5016443015508045 1105 68-69 0.4779315460188016 1105 70-71 0.5767820463084092 1105 72-73 0.5180170516712934 1105 74-75 0.5312193112052697 1105 76-77 0.5436546249702694 1105 78-79 0.6172284089898277 1105 80-81 0.6560257455277707 1105 82-83 0.6637542411033479 1105 84-85 0.6280244602109413 1105 86-87 0.6678019157949429 1105 88-89 0.5831113116197777 1105 90-91 0.6993541495336402 1105 92-93 0.7300318774346017 1105 94-95 0.8172848666868902 1105 96-97 0.7374555404669394 1105 98-99 0.7056731201545077 1105 100 0.7039909109429381 1106 1 0.2623953053205028 1106 2 0.20303435545453397 1106 3 0.23962982155664747 1106 4 0.1623736151864108 1106 5 0.14882177208433234 1106 6 0.12330822636216965 1106 7 0.1750896779519735 1106 8 0.10609611486079729 1106 9 0.14362439192783683 1106 10-11 0.149765900821059 1106 12-13 0.20661989163495775 1106 14-15 0.207251858830368 1106 16-17 0.2631022334337274 1106 18-19 0.24991417228755353 1106 20-21 0.2767511417641586 1106 22-23 0.28185384391024826 1106 24-25 0.2987837645519562 1106 26-27 0.25282443534302246 1106 28-29 0.2940448939038163 1106 30-31 0.3635099965251456 1106 32-33 0.4071200141643061 1106 34-35 0.3695495456538396 1106 36-37 0.4150445206794302 1106 38-39 0.37518652533115215 1106 40-41 0.38713170363995886 1106 42-43 0.29822062705758157 1106 44-45 0.37161285490152096 1106 46-47 0.4413029062251397 1106 48-49 0.4515974352254233 1106 50-51 0.44100510778809365 1106 52-53 0.4360211177468969 1106 54-55 0.4199538247103405 1106 56-57 0.46552024152361327 1106 58-59 0.5374487739403477 1106 60-61 0.5528745458169197 1106 62-63 0.5390523580965265 1106 64-65 0.5853172102111941 1106 66-67 0.5595312923043849 1106 68-69 0.5077828610908881 1106 70-71 0.5938224417603237 1106 72-73 0.5998010621120642 1106 74-75 0.5789261281923359 1106 76-77 0.555958966810671 1106 78-79 0.6126951589995251 1106 80-81 0.6890849657840334 1106 82-83 0.7053074303081104 1106 84-85 0.6411909593752831 1106 86-87 0.5774719311617353 1106 88-89 0.6982717385407575 1106 90-91 0.6127698468032072 1106 92-93 0.6952862525019761 1106 94-95 0.7235402812700862 1106 96-97 0.7642709863105033 1106 98-99 0.8035258989470009 1106 100 0.8241172811612003 1107 1 0.24250471319259503 1107 2 0.19067756330879604 1107 3 0.2638553332120992 1107 4 0.16506598740252088 1107 5 0.1026421991462172 1107 6 0.11607967057232571 1107 7 0.13798964970008853 1107 8 0.16365078934382637 1107 9 0.17702034981003578 1107 10-11 0.14828854153005722 1107 12-13 0.19220470483344343 1107 14-15 0.2653514821895442 1107 16-17 0.258007854972071 1107 18-19 0.19217842712541966 1107 20-21 0.24320630749844696 1107 22-23 0.31596967963451306 1107 24-25 0.3130080246371705 1107 26-27 0.35162241941302597 1107 28-29 0.35478077702954636 1107 30-31 0.2314765866097801 1107 32-33 0.3011463118711575 1107 34-35 0.33067352646165205 1107 36-37 0.40703789248395594 1107 38-39 0.3413761953713248 1107 40-41 0.34645974078672026 1107 42-43 0.31588338857816467 1107 44-45 0.35256117685259625 1107 46-47 0.32254334981879396 1107 48-49 0.45629649452212107 1107 50-51 0.38911740358371816 1107 52-53 0.4002464230742646 1107 54-55 0.4248491572573201 1107 56-57 0.4969952556636841 1107 58-59 0.5457998089048175 1107 60-61 0.5170161376054239 1107 62-63 0.49980056440761444 1107 64-65 0.6232797254208364 1107 66-67 0.6210098100459547 1107 68-69 0.549713446171836 1107 70-71 0.6206433556183697 1107 72-73 0.5583150136047479 1107 74-75 0.6324090560815776 1107 76-77 0.6370976211862072 1107 78-79 0.7268709257076686 1107 80-81 0.6508514932545779 1107 82-83 0.638362766892925 1107 84-85 0.5159170750635198 1107 86-87 0.4622456908500787 1107 88-89 0.5195870783912504 1107 90-91 0.46216851030363415 1107 92-93 0.5711773999160847 1107 94-95 0.6849890927899907 1107 96-97 0.7157209213349454 1107 98-99 0.7456439558997907 1107 100 1.0347992417190959 1108 1 0.2876784335281144 1108 2 0.21561311962648944 1108 3 0.22176777994951635 1108 4 0.163575270120063 1108 5 0.1473479012084553 1108 6 0.13059768181836517 1108 7 0.16420184857876308 1108 8 0.17929254434269382 1108 9 0.17139986911405458 1108 10-11 0.15598029605771302 1108 12-13 0.19840028213861416 1108 14-15 0.26121571591512804 1108 16-17 0.31050800555578206 1108 18-19 0.2923550812823663 1108 20-21 0.30240040601010776 1108 22-23 0.32837447897301075 1108 24-25 0.27680546721354915 1108 26-27 0.36535208907538674 1108 28-29 0.435590655157128 1108 30-31 0.38372249657178514 1108 32-33 0.44497121190201483 1108 34-35 0.44173467860937876 1108 36-37 0.5346777068992594 1108 38-39 0.47129721984865114 1108 40-41 0.40976178343940006 1108 42-43 0.5396566127864517 1108 44-45 0.5830559662464196 1108 46-47 0.6521628176088399 1108 48-49 0.5973441699398592 1108 50-51 0.5499047597148135 1108 52-53 0.49954498980415707 1108 54-55 0.5364450557257854 1108 56-57 0.5967120660916905 1108 58-59 0.6263279008596285 1108 60-61 0.5880222375774409 1108 62-63 0.605305198083542 1108 64-65 0.546753544137708 1108 66-67 0.6489902304756825 1108 68-69 0.6191510477646176 1108 70-71 0.6423096473623247 1108 72-73 0.6455693726446725 1108 74-75 0.666951269305649 1108 76-77 0.6712947723472631 1108 78-79 0.7681189901435665 1108 80-81 0.7851353785847692 1108 82-83 0.7697607102677182 1108 84-85 0.7518109563658548 1108 86-87 0.8247659069694642 1108 88-89 0.8557699882258198 1108 90-91 0.7474203620735373 1108 92-93 0.7976357160910759 1108 94-95 0.8689358959694964 1108 96-97 0.8513942333530018 1108 98-99 0.9730573928019339 1108 100 0.9382539188901546 1109 1 0.11348912266427647 1109 2 0.08671027129557274 1109 3 0.15751167878303818 1109 4 0.07992394372109146 1109 5 0.11825850702471996 1109 6 0.09423089957893893 1109 7 0.11425375552925487 1109 8 0.11736746841407353 1109 9 0.11793713796853922 1109 10-11 0.1003006425744033 1109 12-13 0.14192329421999972 1109 14-15 0.12287310988384093 1109 16-17 0.21103576821503367 1109 18-19 0.20702992371369788 1109 20-21 0.18811356383411493 1109 22-23 0.14097648704966304 1109 24-25 0.19828679750973777 1109 26-27 0.27135540507280353 1109 28-29 0.24002827946502947 1109 30-31 0.16551474782158238 1109 32-33 0.1160939390831146 1109 34-35 0.15761547572543577 1109 36-37 0.22938365990415832 1109 38-39 0.23502476043350384 1109 40-41 0.08922855291206844 1109 42-43 0.2131260716774932 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-0.20534391310975408 2316 80-81 -0.16224805966692912 2316 82-83 -0.0979555665802998 2316 84-85 -0.08875193494553457 2316 86-87 -0.13114833292681638 2316 88-89 0.019748015078103975 2316 90-91 -0.028779375204042168 2316 92-93 0.05582361379992662 2316 94-95 0.13014382199778396 2316 96-97 0.2209819809347593 2316 98-99 0.007623730212586111 2316 100 -0.09073340560615151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 11.0 11 23.0 12 59.0 13 200.0 14 601.0 15 1621.0 16 3820.0 17 7606.0 18 14137.0 19 21610.0 20 30127.0 21 41439.0 22 53388.0 23 61265.0 24 65153.0 25 71047.0 26 81133.0 27 96743.0 28 116100.0 29 138954.0 30 167561.0 31 202268.0 32 247671.0 33 311229.0 34 410335.0 35 556432.0 36 810631.0 37 1264871.0 38 1279510.0 39 209817.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.134551960330676 20.037419437836952 14.070244923021525 23.757783678810853 2 15.136062190366639 21.243328178700196 40.08432153538856 23.536288095544602 3 17.244730533378874 26.759744882161225 31.091018204917848 24.904506379542052 4 12.026879907248583 17.68265423043473 38.09808519844625 32.19238066387044 5 12.422222051971566 38.24810798318094 35.44502138135424 13.884648583493261 6 30.388463037547098 37.88825794152545 18.146704466049666 13.57657455487779 7 26.45599076833206 33.20205126895532 22.74803324294258 17.593924719770037 8 25.52161741140117 36.3964171023986 20.398128108380735 17.68383737781949 9 26.478930518121064 15.265069734696441 20.517289509079166 37.73871023810334 10-11 24.45818217221913 26.496682568842484 29.131889634962864 19.913245623975524 12-13 25.00854695398052 24.40753887627107 28.916635676310655 21.66727849343776 14-15 22.44813172045156 25.83306865175193 26.70421154948056 25.014588078315953 16-17 21.861215089199035 28.578724946002552 27.457892365820385 22.102167598978028 18-19 21.68633315829544 27.637690712829855 29.43629285701745 21.23968327185726 20-21 22.489813335665804 26.910111790146697 29.4405672370124 21.159507637175107 22-23 22.212328362639834 26.831929057887855 29.225355484654585 21.730387094817722 24-25 21.932422865716948 26.83303677018592 29.38823346708668 21.846306897010457 26-27 21.872378452781184 26.947598104122587 29.360762245378737 21.81926119771749 28-29 21.891608738170433 27.251813509213147 29.339194575105992 21.51738317751043 30-31 22.34158711593352 26.92685844180532 29.368150455629422 21.36340398663174 32-33 22.089184487312938 27.02438069830858 29.292710833676566 21.593723980701913 34-35 22.225762102139598 27.204628034747607 29.247501425151928 21.32210843796086 36-37 22.210509582046285 27.189751634037002 29.11891365009654 21.480825133820172 38-39 22.123358549714776 27.273626162363513 29.123567877097134 21.47944741082458 40-41 22.28896826054291 27.080692688204277 29.11356845070926 21.516770600543556 42-43 22.079631447211682 27.19806280573931 28.891615350641196 21.830690396407817 44-45 21.992933661960375 27.332862414387233 28.979392121905384 21.694811801747008 46-47 21.993936755891454 27.21613040651637 28.68364106859051 22.10629176900167 48-49 22.010089177977093 27.31341617088983 28.714132215686888 21.962362435446188 50-51 22.087199339043913 27.547126322295263 28.49565449465463 21.87001984400619 52-53 22.098459673684527 27.643145992020763 28.30432902986026 21.954065304434454 54-55 21.964830229527575 27.515927765559123 28.507749545368466 22.011492459544836 56-57 22.135986112556477 27.56278781873541 28.181903356110904 22.11932271259721 58-59 22.078506115079435 27.554648175435997 28.464911076606402 21.901934632878163 60-61 22.296455684705922 27.62761756156643 28.364706042910846 21.711220710816796 62-63 22.159430031905224 27.616218141266387 28.423386672596386 21.800965154232006 64-65 21.98870290235715 27.41655465815737 28.397249610504076 22.197492828981403 66-67 21.971457061964454 27.472206734297067 28.26041220884452 22.29592399489396 68-69 21.873631941734654 27.52780852160675 28.397515460529966 22.20104407612863 70-71 21.92397912096998 27.35025559285955 28.375723578549035 22.350041707621436 72-73 21.86691305450932 27.6619436211431 28.0674635963 22.40367972804758 74-75 21.900560382136916 27.653363689427408 28.0085963887123 22.43747953972338 76-77 21.746027401042856 27.537447131571792 28.12646269284801 22.590062774537348 78-79 21.693808522044307 27.48302904111618 28.161284784809194 22.66187765203032 80-81 21.755984598877763 27.696920254403913 27.884550003048687 22.66254514366964 82-83 21.874951860509015 27.674638100775862 27.934561420802407 22.51584861791272 84-85 21.835781228411754 27.705142164049878 27.824334224979026 22.63474238255934 86-87 21.608457241088427 27.707212432314897 27.971351550000367 22.712978776596305 88-89 21.559140188342223 27.532346225420945 28.00348913150542 22.905024454731407 90-91 21.84889572178 27.446669923465233 27.923056537384532 22.78137781737023 92-93 21.70259784954958 27.47431245005049 27.912618782141617 22.910470918258312 94-95 21.47866195876509 27.482194481638512 27.89528282696354 23.143860732632852 96-97 21.400700438023208 27.403234786426268 27.976117258513515 23.219947517037006 98-99 21.3379589853322 27.31440640924824 27.85220071268501 23.495433892734553 100 20.546143048502895 27.781579816553894 28.07116267197175 23.601114462971466 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2570.0 1 1975.0 2 2367.5 3 3205.5 4 2374.5 5 1973.5 6 2965.0 7 5734.5 8 9066.5 9 10816.0 10 9367.0 11 7119.0 12 7027.0 13 7473.0 14 7911.0 15 8393.5 16 8976.0 17 9484.5 18 10090.0 19 10982.0 20 12068.5 21 13667.0 22 16061.5 23 19951.0 24 25039.0 25 30943.5 26 38286.5 27 47230.0 28 57481.0 29 68219.0 30 80365.5 31 93283.0 32 106189.0 33 118688.5 34 131115.0 35 142987.5 36 157393.5 37 171957.0 38 182165.5 39 191012.5 40 197004.5 41 201758.5 42 206399.5 43 211019.5 44 216333.5 45 222346.0 46 226615.5 47 229023.0 48 234497.5 49 238724.5 50 238871.5 51 233009.5 52 222546.0 53 212896.0 54 200923.5 55 184656.5 56 166954.5 57 149113.5 58 130209.0 59 109136.0 60 88619.5 61 72210.5 62 58478.5 63 45731.5 64 34119.0 65 25336.5 66 18905.0 67 14194.5 68 10855.0 69 8159.5 70 6149.5 71 4632.0 72 3420.0 73 2625.5 74 2101.5 75 1620.0 76 1150.0 77 733.0 78 482.5 79 340.0 80 227.0 81 154.5 82 114.5 83 83.5 84 52.0 85 46.0 86 33.5 87 19.0 88 15.5 89 14.0 90 11.0 91 8.0 92 4.0 93 2.0 94 1.0 95 1.5 96 1.5 97 1.0 98 2.0 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003144257580134676 2 0.06952480212724188 3 7.980349188159077E-4 4 6.543886334290443E-4 5 0.001883362408405542 6 5.10742348042181E-4 7 0.0 8 0.0011172488863422708 9 1.5960698376318157E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.581331728751123E-4 18-19 0.0012768558701054524 20-21 0.003407609103343926 22-23 0.0 24-25 2.3941047564477232E-5 26-27 9.576419025790893E-5 28-29 8.139956171922258E-4 30-31 0.0016120305360081335 32-33 0.0033278056114623353 34-35 0.004285447514041424 36-37 0.0012608951717291341 38-39 0.0013486790127988841 40-41 0.005570283733335036 42-43 0.007230196364472124 44-45 0.005338853606878422 46-47 0.006815218206687852 48-49 0.00891405004317369 50-51 0.00839532734594335 52-53 0.004093919133525607 54-55 0.0024180458040122003 56-57 0.002585633136963541 58-59 0.00354327503954263 60-61 0.007509508586057692 62-63 0.01434866784031002 64-65 0.003846528308692675 66-67 0.010422336039735754 68-69 0.012904224637253226 70-71 0.005554323034958717 72-73 0.007509508586057692 74-75 0.003208100373639949 76-77 0.003040513040688608 78-79 0.007924486743841963 80-81 0.006256593763516716 82-83 0.016639028057311676 84-85 0.018043569514427673 86-87 0.023238776835919232 88-89 0.02181827468042692 90-91 0.0250582964508195 92-93 0.03496190979332491 94-95 0.03900794683172157 96-97 0.023182914391602118 98-99 0.01902515246457124 100 0.019264562940216015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6265390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.29481135996499 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63745463464656 46.49526156853252 2 16.032728718694113 19.975116222330854 3 4.773054130405631 8.920095199935615 4 1.8663782410120056 4.650627218007446 5 0.8763713460414633 2.729669384146577 6 0.48834828507981876 1.8252938578205826 7 0.3123433395180077 1.3620158590371052 8 0.20331698522527689 1.013247459270833 9 0.14128260947369808 0.7921056155046862 >10 0.6195463362109805 6.615504959581231 >50 0.03252250645576428 1.391390258106904 >100 0.015134914085345898 1.8072376515807353 >500 9.569711673775447E-4 0.4267767853939536 >1k 4.619851632004061E-4 0.673537902205741 >5k 0.0 0.0 >10k+ 9.899682068562979E-5 1.3221200585450579 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24941 0.39807577820375106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23549 0.3758584860639162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15826 0.25259401250361113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.192139675263631E-5 2 0.0 1.5960698376318154E-5 0.0 0.0 3.192139675263631E-5 3 0.0 1.5960698376318154E-5 0.0 0.0 3.192139675263631E-5 4 0.0 1.5960698376318154E-5 0.0 0.0 3.192139675263631E-5 5 0.0 1.5960698376318154E-5 0.0 0.0 3.192139675263631E-5 6 0.0 1.5960698376318154E-5 0.0 0.0 4.7882095128954464E-5 7 0.0 1.5960698376318154E-5 0.0 0.0 4.7882095128954464E-5 8 0.0 1.5960698376318154E-5 0.0 0.0 7.980349188159076E-5 9 0.0 1.5960698376318154E-5 0.0 0.0 1.1172488863422708E-4 10-11 0.0 1.5960698376318154E-5 0.0 0.0 1.1172488863422708E-4 12-13 0.0 1.5960698376318154E-5 0.0 0.0 1.1172488863422708E-4 14-15 0.0 1.5960698376318154E-5 0.0 0.0 1.1970523782238615E-4 16-17 0.0 1.5960698376318154E-5 0.0 0.0 1.4364628538686338E-4 18-19 0.0 1.5960698376318154E-5 0.0 0.0 1.9152838051581786E-4 20-21 0.0 1.5960698376318154E-5 0.0 2.3941047564477232E-5 1.9152838051581786E-4 22-23 0.0 1.5960698376318154E-5 0.0 7.980349188159078E-5 2.154694280802951E-4 24-25 0.0 1.5960698376318154E-5 0.0 2.154694280802951E-4 2.394104756447723E-4 26-27 0.0 3.192139675263631E-5 0.0 3.830567610316357E-4 2.473908248329314E-4 28-29 7.980349188159077E-6 4.7882095128954464E-5 0.0 0.0013167576160462476 2.5537117402109046E-4 30-31 1.5960698376318154E-5 4.7882095128954464E-5 0.0 0.005027619988540219 2.633515232092496E-4 32-33 1.5960698376318154E-5 5.586244431711354E-5 0.0 0.012768558701054523 3.0325326915004494E-4 34-35 1.5960698376318154E-5 6.384279350527261E-5 0.0 0.024675239689787867 3.1921396752636306E-4 36-37 1.5960698376318154E-5 6.384279350527261E-5 0.0 0.045216658500109336 3.1921396752636306E-4 38-39 1.5960698376318154E-5 7.980349188159076E-5 0.0 0.07880594823307088 3.271943167145222E-4 40-41 1.5960698376318154E-5 7.980349188159076E-5 0.0 0.11734305446269107 3.3517466590268124E-4 42-43 1.5960698376318154E-5 8.778384106974984E-5 0.0 0.15391699479202414 3.591157134671585E-4 44-45 1.5960698376318154E-5 9.576419025790893E-5 0.0 0.20137613141400615 3.830567610316357E-4 46-47 1.5960698376318154E-5 9.576419025790893E-5 0.0 0.25810843379262904 3.9901745940795384E-4 48-49 1.5960698376318154E-5 1.03744539446068E-4 0.0 0.3201476683813777 3.9901745940795384E-4 50-51 1.5960698376318154E-5 1.1172488863422708E-4 0.0 0.3890819246686958 3.9901745940795384E-4 52-53 1.5960698376318154E-5 1.1172488863422708E-4 0.0 0.4611604385361486 4.069978085961129E-4 54-55 1.5960698376318154E-5 1.1172488863422708E-4 0.0 0.5452014958366518 4.14978157784272E-4 56-57 1.5960698376318154E-5 1.1172488863422708E-4 0.0 0.6417557406641885 4.229585069724311E-4 58-59 1.5960698376318154E-5 1.1970523782238615E-4 0.0 0.753448707901663 4.3093885616059014E-4 60-61 1.5960698376318154E-5 1.2768558701054523E-4 0.0 0.8731938474699898 4.3093885616059014E-4 62-63 1.5960698376318154E-5 1.5960698376318153E-4 0.0 0.9983656244862651 4.3093885616059014E-4 64-65 1.5960698376318154E-5 1.5960698376318153E-4 0.0 1.1279345739052158 4.468995545369083E-4 66-67 1.5960698376318154E-5 1.5960698376318153E-4 0.0 1.2811093962227411 4.7084060210138555E-4 68-69 1.5960698376318154E-5 1.5960698376318153E-4 0.0 1.458392853437695 4.8680130047770373E-4 70-71 1.5960698376318154E-5 1.5960698376318153E-4 0.0 1.6478623038629678 4.947816496658628E-4 72-73 1.5960698376318154E-5 1.755676821394997E-4 0.0 1.8374833809228157 5.26703046418499E-4 74-75 1.5960698376318154E-5 1.9152838051581786E-4 0.0 2.032650800668434 5.26703046418499E-4 76-77 1.5960698376318154E-5 1.9152838051581786E-4 0.0 2.2532994753718443 5.26703046418499E-4 78-79 1.5960698376318154E-5 1.9152838051581786E-4 0.0 2.5027252892477563 5.26703046418499E-4 80-81 1.5960698376318154E-5 1.9152838051581786E-4 0.0 2.7726366594896725 5.26703046418499E-4 82-83 1.5960698376318154E-5 1.9152838051581786E-4 0.0 3.054622298053274 5.426637447948173E-4 84-85 1.5960698376318154E-5 1.9152838051581786E-4 0.0 3.344596266154222 5.506440939829763E-4 86-87 1.5960698376318154E-5 1.9152838051581786E-4 0.0 3.653100605070075 5.666047923592945E-4 88 1.5960698376318154E-5 1.9152838051581786E-4 0.0 3.9024865172000465 5.745851415474535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12390 0.0 49.45678 1 GTATCAA 20220 0.0 35.25906 1 TACCTGG 5405 0.0 29.930124 2 TCAACGC 25410 0.0 27.593376 4 ATCAACG 25595 0.0 27.339283 3 CAACGCA 25795 0.0 27.126663 5 TATCAAC 26370 0.0 26.661026 2 AACGCAG 26540 0.0 26.523918 6 GTACCTG 6385 0.0 26.441511 1 GTACATG 23525 0.0 25.487854 1 TACATGG 23965 0.0 24.293438 2 ACATGGG 23235 0.0 23.846176 3 CATGGGG 13765 0.0 23.795631 4 ACCTGGG 6820 0.0 23.221653 3 ACGCAGA 30425 0.0 22.889927 7 CGCAGAG 30995 0.0 22.423498 8 ATGGGGG 8880 0.0 21.750341 5 GAGTACT 21140 0.0 20.262629 12-13 GCAGAGT 34070 0.0 19.971758 9 AGAGTAC 32030 0.0 19.1095 10-11 >>END_MODULE