FastQCFastQC Report
Mon 6 Jun 2016
ERR1211479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085204
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207420.6723056238744666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174360.5651490144573907No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA153450.49737391757562865No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137960.4471665406890436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.3952088743564445No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70140.2273431513766999No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG65330.21175261020016828No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.2050431673237815No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT57900.1876699239337172No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32140.10417463480534837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG87650.073.884012
ACCTGGG91000.068.991463
TATAACG7000.065.8412
GTACCTG101900.064.2393951
TAACGCA7850.059.8603864
TACCCGG6100.057.051922
GTACCCG6700.056.231031
CCTGGGG48450.054.6039124
ATAACGC10300.046.9926873
TATCACG3450.043.6213722
CCTGGGA61300.042.1610454
CTGGGGG34450.041.191995
GTATAAC14200.036.1492231
ACCCGGG9650.034.574933
ATGGGGG65550.032.042765
TGGGGGG85900.031.99026
GTGGTTA23700.031.594361
CATGGGG96500.031.408094
TGGGATG39500.031.1572066
GGATGAC24050.031.0578588