##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3085204 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43325271197626 31.0 31.0 34.0 30.0 34.0 2 31.408443331462035 31.0 31.0 34.0 28.0 34.0 3 31.493100294178276 33.0 31.0 34.0 28.0 34.0 4 35.07649445547199 37.0 35.0 37.0 32.0 37.0 5 35.194579353585695 37.0 35.0 37.0 32.0 37.0 6 35.3281533409136 37.0 35.0 37.0 33.0 37.0 7 35.28644037801066 37.0 35.0 37.0 32.0 37.0 8 35.25581290572682 37.0 35.0 37.0 32.0 37.0 9 36.83700591597833 39.0 37.0 39.0 33.0 39.0 10-11 36.79543184178421 39.0 37.0 39.0 32.0 39.0 12-13 36.73649505834946 39.0 37.0 39.0 32.0 39.0 14-15 37.89409598198369 40.0 38.0 41.0 32.0 41.0 16-17 37.85714964067206 40.0 38.0 41.0 32.0 41.0 18-19 37.90954455523848 40.0 38.0 41.0 32.0 41.0 20-21 37.86187056026117 40.0 37.5 41.0 32.0 41.0 22-23 37.68794575658531 40.0 37.0 41.0 32.0 41.0 24-25 37.614293414633195 40.0 37.0 41.0 32.0 41.0 26-27 37.45518805239459 40.0 37.0 41.0 32.0 41.0 28-29 37.266756266360346 40.0 36.0 41.0 31.0 41.0 30-31 37.01457310440412 39.5 36.0 41.0 30.5 41.0 32-33 36.67003154410535 39.0 35.0 41.0 30.0 41.0 34-35 36.43586907057038 39.0 35.0 41.0 30.0 41.0 36-37 36.23324016823523 39.0 35.0 41.0 29.0 41.0 38-39 36.08266228100314 39.0 35.0 40.0 28.0 41.0 40-41 36.01110331764123 39.0 35.0 40.0 28.0 41.0 42-43 35.93278191652804 39.0 35.0 40.0 27.0 41.0 44-45 35.716905429916466 39.0 35.0 40.0 26.5 41.0 46-47 35.55659625749221 39.0 35.0 40.0 24.5 41.0 48-49 35.443212507179425 39.0 35.0 41.0 24.0 41.0 50-51 34.454124103300785 38.0 34.0 40.0 23.0 40.5 52-53 34.67200159211514 38.0 33.5 39.5 23.0 40.5 54-55 35.271152410018914 39.0 35.0 40.5 24.0 41.0 56-57 35.2377630458148 39.0 34.5 41.0 23.5 41.0 58-59 35.09410722921402 39.0 34.0 41.0 23.0 41.0 60-61 34.88889891883973 38.0 34.0 40.0 23.0 41.0 62-63 34.5698608584716 38.0 34.0 40.0 22.0 41.0 64-65 34.32582578007808 37.5 33.5 40.0 22.0 41.0 66-67 34.03209155699267 37.0 33.0 40.0 22.0 41.0 68-69 33.745286373283584 36.5 33.0 39.0 21.5 41.0 70-71 33.37054032731709 36.0 33.0 39.0 20.5 41.0 72-73 33.00795733442586 35.5 32.0 39.0 20.0 40.0 74-75 32.632763506076095 35.0 32.0 37.5 20.0 40.0 76-77 32.252006512373256 35.0 32.0 37.0 19.0 39.0 78-79 31.915051160312252 35.0 31.0 36.5 18.5 39.0 80-81 31.59657627177976 35.0 31.0 36.0 18.0 38.5 82-83 31.296687998589398 35.0 31.0 36.0 18.0 37.0 84-85 31.01214085032951 35.0 31.0 35.0 17.5 37.0 86-87 30.703694472067326 34.5 30.0 35.0 16.0 36.5 88-89 30.485873543532293 34.0 30.0 35.0 15.0 36.0 90-91 30.242632902070657 34.0 29.5 35.0 13.5 36.0 92-93 30.031144293861928 34.0 29.0 35.0 11.5 36.0 94-95 29.875590884751865 34.0 29.0 35.0 11.0 35.0 96-97 29.762805636191317 34.0 29.0 35.0 10.0 35.0 98-99 29.56137535799902 34.0 29.0 35.0 10.0 35.0 100 28.23965903065081 33.0 25.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3739420372958868 1101 2 0.3902159413632127 1101 3 0.418008744897552 1101 4 0.3757467125695513 1101 5 0.39682753254285785 1101 6 0.41678922010219566 1101 7 0.44650530559366786 1101 8 0.491774868629939 1101 9 0.47820161444988685 1101 10-11 0.501966210698015 1101 12-13 0.4388709473167154 1101 14-15 0.4940177452447969 1101 16-17 0.4368089805893405 1101 18-19 0.48680616042849323 1101 20-21 0.5162543622948021 1101 22-23 0.4824590349332638 1101 24-25 0.4515775175728649 1101 26-27 0.4142635782923776 1101 28-29 0.5096382505071162 1101 30-31 0.5296130761253934 1101 32-33 0.4458970871598069 1101 34-35 0.4322575048847028 1101 36-37 0.39288192674123223 1101 38-39 0.3255675246694594 1101 40-41 0.36253476780073157 1101 42-43 0.17305262331444027 1101 44-45 0.2103419295581901 1101 46-47 0.22206632116331093 1101 48-49 0.1798823157827414 1101 50-51 0.20947099253927348 1101 52-53 0.329142596396764 1101 54-55 0.266812735489772 1101 56-57 0.29486176580473966 1101 58-59 0.23866156774525393 1101 60-61 0.1572446004852921 1101 62-63 0.22337996081638778 1101 64-65 0.25159205493805814 1101 66-67 0.1706986501917953 1101 68-69 0.10207251724824573 1101 70-71 0.18422795244124046 1101 72-73 0.04258121289965544 1101 74-75 0.14014959285236017 1101 76-77 0.09914167550979158 1101 78-79 -0.017561169240195795 1101 80-81 0.054237459800308585 1101 82-83 0.07393700794105129 1101 84-85 -0.057218867758454195 1101 86-87 -0.17745520191584774 1101 88-89 -0.17205525910968333 1101 90-91 -0.22269617067293268 1101 92-93 -0.3202369672962355 1101 94-95 -0.1678736842020463 1101 96-97 -0.18273199459953204 1101 98-99 -0.22294605495249442 1101 100 -0.23560083754505712 1102 1 0.297753224087856 1102 2 0.2858214647775199 1102 3 0.16408529184962006 1102 4 0.23575516129989893 1102 5 0.24450992939945593 1102 6 0.2721837889964078 1102 7 0.2715902135147843 1102 8 0.2725797898107416 1102 9 0.2925298971886008 1102 10-11 0.2398811840945143 1102 12-13 0.263105707430519 1102 14-15 0.30880156824526495 1102 16-17 0.24538616983590344 1102 18-19 0.26897524689702124 1102 20-21 0.40003125397143435 1102 22-23 0.35790792488923984 1102 24-25 0.3858401657723718 1102 26-27 0.3050186301374751 1102 28-29 0.3693012925866128 1102 30-31 0.29509282804738746 1102 32-33 0.31732382670926995 1102 34-35 0.3748490642484015 1102 36-37 0.36898766228323865 1102 38-39 0.42520370178721834 1102 40-41 0.3803753669057457 1102 42-43 0.34103075301654684 1102 44-45 0.30593863137030297 1102 46-47 0.4466966892105546 1102 48-49 0.5429713225658332 1102 50-51 0.445715454596602 1102 52-53 0.49435642703200955 1102 54-55 0.508692655295043 1102 56-57 0.49350146565198827 1102 58-59 0.5310533905747405 1102 60-61 0.43331159373313 1102 62-63 0.46744763796575484 1102 64-65 0.5186331395040327 1102 66-67 0.3895894746796529 1102 68-69 0.374269055269572 1102 70-71 0.35273374127663715 1102 72-73 0.34626473200956553 1102 74-75 0.367365054119837 1102 76-77 0.44082938442602426 1102 78-79 0.19054615600909486 1102 80-81 0.26167811747349035 1102 82-83 0.2115141685401376 1102 84-85 0.09521023316112931 1102 86-87 0.1491545244917063 1102 88-89 0.13247222359156652 1102 90-91 4.3284253082376267E-4 1102 92-93 0.002930015174694489 1102 94-95 -0.022168917763060847 1102 96-97 -0.036687778364782986 1102 98-99 -0.11855344272602863 1102 100 0.036710563193501855 1103 1 0.35920484455206037 1103 2 0.40434449813841766 1103 3 0.36189415621484855 1103 4 0.4308512359696195 1103 5 0.30984255502531965 1103 6 0.32558999591829973 1103 7 0.29450033048487967 1103 8 0.3625355830004011 1103 9 0.3279688203702307 1103 10-11 0.3986165190231219 1103 12-13 0.36941722876765226 1103 14-15 0.5086286798282984 1103 16-17 0.39747629526183914 1103 18-19 0.42692379542510395 1103 20-21 0.431171405758505 1103 22-23 0.48190434970336327 1103 24-25 0.4852964158674382 1103 26-27 0.47381740344452083 1103 28-29 0.490446472868328 1103 30-31 0.46553526886251007 1103 32-33 0.563206140720574 1103 34-35 0.5780396988127592 1103 36-37 0.5800981536907273 1103 38-39 0.5094603861136662 1103 40-41 0.45756449443390323 1103 42-43 0.38533812435562 1103 44-45 0.3094529736102061 1103 46-47 0.4641436887612542 1103 48-49 0.6217530587672471 1103 50-51 0.622811599894753 1103 52-53 0.5256681240506609 1103 54-55 0.5775333964736262 1103 56-57 0.5386899132259089 1103 58-59 0.5522869275474349 1103 60-61 0.6078370512322948 1103 62-63 0.6785976688367867 1103 64-65 0.5622673614834781 1103 66-67 0.5961806810140118 1103 68-69 0.5818304239416747 1103 70-71 0.5831193431121875 1103 72-73 0.5315273109628507 1103 74-75 0.542379840278052 1103 76-77 0.6126318226721921 1103 78-79 0.5734368030179304 1103 80-81 0.48269228825710897 1103 82-83 0.5366912088231572 1103 84-85 0.4100218407378726 1103 86-87 0.45919982541993676 1103 88-89 0.4303964773653597 1103 90-91 0.41506536168922636 1103 92-93 0.32624349693979937 1103 94-95 0.32169049680559425 1103 96-97 0.28695771925559654 1103 98-99 0.2904690536720551 1103 100 0.3030001772660995 1104 1 0.3801043212440547 1104 2 0.3747020024769512 1104 3 0.4041939744802683 1104 4 0.38504456893095806 1104 5 0.3179905927792106 1104 6 0.22603733985999241 1104 7 0.31432779917575004 1104 8 0.41547964081643585 1104 9 0.4047000311830473 1104 10-11 0.3861266369704879 1104 12-13 0.34219549174387254 1104 14-15 0.3679591047962134 1104 16-17 0.3988927637485844 1104 18-19 0.364897908665597 1104 20-21 0.3760505324860617 1104 22-23 0.4336152981346757 1104 24-25 0.4734805339037962 1104 26-27 0.5130102790675792 1104 28-29 0.5534608018594867 1104 30-31 0.4544042907095047 1104 32-33 0.41506919386151253 1104 34-35 0.3379596575498951 1104 36-37 0.40784826652437545 1104 38-39 0.5349406177987674 1104 40-41 0.31647294095993317 1104 42-43 0.1255988937010315 1104 44-45 0.30517487414565636 1104 46-47 0.3125852246833176 1104 48-49 0.29359142976712604 1104 50-51 0.40726078272482624 1104 52-53 0.4819932890985754 1104 54-55 0.43711840347673103 1104 56-57 0.4531741874989166 1104 58-59 0.5030087274331478 1104 60-61 0.5371103477619599 1104 62-63 0.4863995774383483 1104 64-65 0.4598794729313411 1104 66-67 0.3899468511890518 1104 68-69 0.27829160563316435 1104 70-71 0.3102867734219643 1104 72-73 0.3949242943665965 1104 74-75 0.4101573828140559 1104 76-77 0.4666951762084537 1104 78-79 0.31375438649188325 1104 80-81 0.3119504696681936 1104 82-83 0.3461056321778493 1104 84-85 0.24019090227971063 1104 86-87 0.35065062782790335 1104 88-89 0.3969965680560712 1104 90-91 0.24215467367586996 1104 92-93 0.20415549693132462 1104 94-95 0.2970551048966996 1104 96-97 0.3292186461090161 1104 98-99 0.2775457597204358 1104 100 0.30495922730208846 1105 1 0.3435565209185647 1105 2 0.3482012294748671 1105 3 0.31188549065513627 1105 4 0.29049234293220394 1105 5 0.29394535579553605 1105 6 0.31040110024243717 1105 7 0.2742527677975417 1105 8 0.3232804171635877 1105 9 0.4425634985082567 1105 10-11 0.4132247049743114 1105 12-13 0.40168092905361874 1105 14-15 0.44575071128797816 1105 16-17 0.3846008605636442 1105 18-19 0.3753253468217963 1105 20-21 0.4541111080708333 1105 22-23 0.49724734986982355 1105 24-25 0.43657541100155584 1105 26-27 0.5074444640848625 1105 28-29 0.5666672843145903 1105 30-31 0.5135505851716715 1105 32-33 0.4719074808074524 1105 34-35 0.44409827943916724 1105 36-37 0.5080890206777227 1105 38-39 0.4420810154839785 1105 40-41 0.5416982423434789 1105 42-43 0.49685941431030045 1105 44-45 0.5312915276502039 1105 46-47 0.5699980092506252 1105 48-49 0.5644394886810815 1105 50-51 0.632894037230848 1105 52-53 0.6130627734778216 1105 54-55 0.5495063072666966 1105 56-57 0.560600388706348 1105 58-59 0.5000997889693508 1105 60-61 0.6816418306897418 1105 62-63 0.6616132619749564 1105 64-65 0.5517520068510748 1105 66-67 0.49776855240448015 1105 68-69 0.4956552649363104 1105 70-71 0.524399806251644 1105 72-73 0.45976493449666833 1105 74-75 0.39588674991222916 1105 76-77 0.37338153515775474 1105 78-79 0.3547785815298319 1105 80-81 0.41773440504143977 1105 82-83 0.3891890451383979 1105 84-85 0.3080513772214992 1105 86-87 0.2608099513383664 1105 88-89 0.4489456895388386 1105 90-91 0.3262546461881186 1105 92-93 0.3513108601695869 1105 94-95 0.29782801608217824 1105 96-97 0.19590423787374434 1105 98-99 0.2470455372005489 1105 100 0.22692837557450218 1106 1 0.2788067574817461 1106 2 0.26255052823500336 1106 3 0.2579860172775206 1106 4 0.24683461446476684 1106 5 0.20736966592916417 1106 6 0.22297624507618252 1106 7 0.2339363019367795 1106 8 0.2867209831469495 1106 9 0.33820290179266266 1106 10-11 0.25897655621062654 1106 12-13 0.32194822446633253 1106 14-15 0.3346018284089993 1106 16-17 0.3155943587833292 1106 18-19 0.30378435941742055 1106 20-21 0.3015678649924354 1106 22-23 0.4126215225981653 1106 24-25 0.3434008533929003 1106 26-27 0.4290311133597342 1106 28-29 0.4255221618249436 1106 30-31 0.5058277974920884 1106 32-33 0.389242427974537 1106 34-35 0.5021425748110317 1106 36-37 0.48250900020821774 1106 38-39 0.554278834346249 1106 40-41 0.4433635016620556 1106 42-43 0.37635558369346 1106 44-45 0.4143390825857409 1106 46-47 0.523188856283916 1106 48-49 0.4996935024210529 1106 50-51 0.5605792881668918 1106 52-53 0.5390331861525794 1106 54-55 0.4669419791481033 1106 56-57 0.5054772499451801 1106 58-59 0.3595546780070862 1106 60-61 0.41398492816496457 1106 62-63 0.5693256414340482 1106 64-65 0.7268552077146211 1106 66-67 0.5955046805128887 1106 68-69 0.631850746985215 1106 70-71 0.5963448116767864 1106 72-73 0.4193726453093021 1106 74-75 0.44638074867778954 1106 76-77 0.5254681595312363 1106 78-79 0.3650225647669991 1106 80-81 0.32720275202017746 1106 82-83 0.35410326965043026 1106 84-85 0.3824950550389552 1106 86-87 0.391304554452212 1106 88-89 0.5186229899374304 1106 90-91 0.5083288366551955 1106 92-93 0.48247738106520544 1106 94-95 0.51113582129625 1106 96-97 0.5550342897078018 1106 98-99 0.5880156225199791 1106 100 0.6416671432300092 1107 1 0.14366558197542645 1107 2 0.2264429168634976 1107 3 0.13871053953586454 1107 4 0.1827751450035322 1107 5 0.12129964502502588 1107 6 0.19108826793292621 1107 7 0.1640850354299772 1107 8 0.17439276052144237 1107 9 0.10654316932509289 1107 10-11 0.19632695594253846 1107 12-13 0.29056825356676086 1107 14-15 0.20580402160332056 1107 16-17 0.24885359918388872 1107 18-19 0.21854403066675587 1107 20-21 0.32138450583718736 1107 22-23 0.34134139219749926 1107 24-25 0.30172589071136713 1107 26-27 0.30342754427813645 1107 28-29 0.2554782011061718 1107 30-31 0.30570407353704354 1107 32-33 0.320658910406209 1107 34-35 0.37233115818897744 1107 36-37 0.39034757372161266 1107 38-39 0.37474835796096784 1107 40-41 0.3782978117310094 1107 42-43 0.2989720796888875 1107 44-45 0.2688776645855526 1107 46-47 0.29293436900120895 1107 48-49 0.4098652961155764 1107 50-51 0.2902343770421467 1107 52-53 0.21303983130582083 1107 54-55 0.08583806154321394 1107 56-57 0.26775294927657 1107 58-59 0.20318277645530713 1107 60-61 0.16885351022279593 1107 62-63 0.3870544881374798 1107 64-65 0.37673536429986143 1107 66-67 0.4299650478658634 1107 68-69 0.3038235084937284 1107 70-71 0.2519241491416295 1107 72-73 0.2803789002325132 1107 74-75 0.3494095375720505 1107 76-77 0.33052019685114686 1107 78-79 0.274547405302183 1107 80-81 0.36813111869714277 1107 82-83 0.34267979317955977 1107 84-85 0.17727522061834478 1107 86-87 0.1606098797140234 1107 88-89 0.21184538152528987 1107 90-91 0.2386535453970744 1107 92-93 0.3238824195296992 1107 94-95 0.3357742963817323 1107 96-97 0.24123163404011194 1107 98-99 0.2281231189259465 1107 100 0.46965258431325196 1108 1 0.26934983508434485 1108 2 0.31598288756016757 1108 3 0.287475463112834 1108 4 0.24340579463085277 1108 5 0.18635605533901156 1108 6 0.2276573468849108 1108 7 0.1997846980487239 1108 8 0.23526874484247884 1108 9 0.19214155792261778 1108 10-11 0.2762831728657744 1108 12-13 0.19609887119619174 1108 14-15 0.28736008913290334 1108 16-17 0.3731943444259542 1108 18-19 0.30704617508669685 1108 20-21 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-0.19881735511512488 2316 58-59 -0.3830494691773154 2316 60-61 -0.26760567741283126 2316 62-63 -0.42339055950991167 2316 64-65 -0.16438772417984637 2316 66-67 -0.14485182903476357 2316 68-69 -0.16761863240898833 2316 70-71 -0.2298573932112049 2316 72-73 -0.19981336768152858 2316 74-75 -0.058163557439669944 2316 76-77 -0.17987545246698033 2316 78-79 -0.2862639792663053 2316 80-81 -0.04144961695239502 2316 82-83 -0.0016966901311512572 2316 84-85 -0.03242789950533265 2316 86-87 0.06670152382105954 2316 88-89 -0.06148192481874659 2316 90-91 0.03205798389819492 2316 92-93 0.09350294600974607 2316 94-95 0.12621805577149559 2316 96-97 -0.06223309854663839 2316 98-99 0.14430204702302518 2316 100 -0.04784086553179634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 12.0 1 32.0 2 39.0 3 50.0 4 60.0 5 58.0 6 111.0 7 774.0 8 1789.0 9 2417.0 10 3267.0 11 4454.0 12 5851.0 13 7003.0 14 8143.0 15 9145.0 16 12126.0 17 17664.0 18 24639.0 19 28688.0 20 31735.0 21 36297.0 22 40465.0 23 40131.0 24 36754.0 25 34282.0 26 35737.0 27 38495.0 28 44587.0 29 51745.0 30 62039.0 31 76371.0 32 94989.0 33 125119.0 34 178486.0 35 242740.0 36 358702.0 37 600273.0 38 660182.0 39 169415.0 40 338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.73308926850681 17.055631456051447 11.347780689701747 21.86349858574 2 15.615436529052188 20.757777316322958 42.8227407820794 20.804045372545456 3 17.890651900856028 32.072775093523795 29.548362288043545 20.488210717576635 4 11.045061636329445 17.02540674789736 38.11939485510113 33.810136760672066 5 10.61536177039307 37.70132398198378 34.18250694153548 17.50080730608767 6 25.47824750949558 35.470798847888474 23.970363532286285 15.080590110329654 7 26.196716699091688 32.8785186443337 24.518697493019598 16.40606716355501 8 27.676363773093676 33.122153201056456 22.774197061695066 16.427285964154805 9 29.998862246586494 15.153643712032341 21.62395985513255 33.223534186248614 10-11 23.91571414262788 26.423016295689305 30.292744134696076 19.368525426986736 12-13 25.0333026954478 24.83441846338065 29.573474279492395 20.558804561679153 14-15 22.95537713816664 25.340913658980007 28.977693431868488 22.726015770984866 16-17 20.796644846042472 27.216297443811854 30.42684499188068 21.560212718264996 18-19 20.5738977475969 26.25872016406341 32.38728664187086 20.780095446468835 20-21 21.468714273232965 26.552972102303386 32.24941439102745 19.72889923343619 22-23 20.557892678548324 26.626993765161888 31.430578691686005 21.38453486460378 24-25 20.07481763626647 26.584947225832785 31.741784980421922 21.598450157478823 26-27 20.148390715863563 25.92644518219655 32.04938966347817 21.87577443846171 28-29 20.300434611338154 26.85453894075442 32.041765484953956 20.803260962953477 30-31 22.137656869584614 26.416915851251716 32.08832350858812 19.35710377057556 32-33 20.793757314633808 26.404442110245512 32.49469131840094 20.307109256719738 34-35 20.585219807963355 27.339667554689594 31.878294120650626 20.196818516696425 36-37 21.25096894606873 27.060917084152337 31.287710278722543 20.400403691056386 38-39 21.329492071277855 27.831875785865577 30.590179883689057 20.248452259167507 40-41 21.035147061827274 27.145396664422027 31.498763285882458 20.320692987868238 42-43 21.100343310121747 27.217184182573025 30.331277406769967 21.351195100535264 44-45 20.48680808235073 27.63690413366803 30.99248272186849 20.883805062112753 46-47 20.28360884802547 26.644772461282596 31.253189302746286 21.818429387945653 48-49 20.525254555415444 27.22998972377374 31.03449505476872 21.210260666042096 50-51 20.303715235765072 27.800852406033133 31.25394778749659 20.64148457070521 52-53 20.193730756524506 28.386568009526382 29.501372513484412 21.9183287204647 54-55 20.13287007234711 28.097179761224872 30.215021463888935 21.55492870253908 56-57 20.911635838440752 27.826743803421227 29.47167590892071 21.789944449217312 58-59 20.50431570065597 28.168471595970342 30.160526718924647 21.16668598444905 60-61 21.766401043709045 28.426725007858135 29.95453387863932 19.852340069793502 62-63 20.726585393379402 28.192466049290633 30.47980145207466 20.601147105255308 64-65 20.36146271737881 27.761084371940576 30.083731097521138 21.793721813159475 66-67 20.816831021131982 27.59760969414427 29.73189703173161 21.853662252992137 68-69 20.53408262492845 27.993404089795032 30.063720370238585 21.40879291503794 70-71 20.369887277930225 27.864052563099634 30.39463668310952 21.371423475860617 72-73 20.362893811852295 28.627641084838974 29.26798661461238 21.74147848869635 74-75 21.004190422826408 28.215102967408217 29.351199457057653 21.429507152707725 76-77 20.368722339421538 27.63175683233106 30.555433979501956 21.444086848745446 78-79 20.49825377441158 27.89073843962352 30.06702860905262 21.54397917691228 80-81 20.0205252432773 28.528726506077412 29.43903774184068 22.0117105088046 82-83 20.710160841108255 28.784327417811795 28.804492126524146 21.701019614555808 84-85 20.49221941256565 29.379368475653244 28.152888543085 21.975523568696104 86-87 20.215646137646278 28.77118951780231 29.134403659017345 21.87876068553407 88-89 20.081756558411655 28.315182409129346 29.59865242213408 22.004408610324912 90-91 21.533689561473672 28.40774061853491 29.120369814998476 20.938200004992943 92-93 20.42101676308571 28.783463314103564 29.245503087911246 21.550016834899484 94-95 20.182618202979178 28.433521284519518 29.14172200244604 22.242138510055266 96-97 20.86896084241415 28.214166869411123 29.03426481240722 21.882607475767514 98-99 20.9392458185455 28.06363218599479 28.872326337023978 22.12479565843573 100 20.372626417323815 28.6382179926476 28.987192486898866 22.00196310312971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2344.0 1 1842.0 2 4801.5 3 7112.5 4 4315.5 5 2921.5 6 3713.5 7 5979.5 8 8868.5 9 10105.5 10 8709.0 11 7064.5 12 7131.0 13 7666.5 14 8145.5 15 8508.0 16 8932.0 17 9361.5 18 9889.0 19 10364.5 20 10834.0 21 11655.5 22 12751.0 23 14456.0 24 16412.5 25 18954.0 26 22673.0 27 26254.0 28 29461.5 29 34378.5 30 41250.5 31 47265.5 32 54765.0 33 64985.5 34 75271.0 35 83141.5 36 93878.5 37 103932.5 38 105874.0 39 106556.0 40 102257.0 41 96755.5 42 93028.0 43 89464.0 44 90091.0 45 93460.0 46 94993.5 47 93919.5 48 98564.0 49 112412.5 50 140760.5 51 142996.0 52 112336.0 53 93245.5 54 84332.5 55 75386.0 56 66375.0 57 60106.0 58 53558.5 59 44417.5 60 33115.0 61 23961.0 62 18406.5 63 14038.5 64 10460.5 65 7784.0 66 5804.5 67 4530.0 68 3598.5 69 2794.5 70 2209.0 71 1684.0 72 1340.0 73 1151.5 74 957.5 75 753.5 76 575.5 77 444.5 78 339.5 79 268.0 80 234.5 81 184.5 82 142.0 83 121.5 84 98.5 85 86.5 86 74.5 87 55.0 88 41.5 89 30.0 90 23.0 91 19.0 92 14.0 93 9.5 94 8.0 95 8.0 96 7.0 97 6.5 98 4.5 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16686092718666254 2 0.10255399642940953 3 0.031181082353063203 4 0.03189416323847629 5 0.028263933276373297 6 0.0027874980066147975 7 0.004472961917591187 8 0.005186042803004274 9 0.005737059850823479 10-11 0.005023978965410391 12-13 0.00789250889082213 14-15 0.01789184767036475 16-17 0.009026955753979316 18-19 0.017600132762695755 20-21 0.019269390289912758 22-23 0.04547511282884373 24-25 0.016319828445704076 26-27 0.00996692601202384 28-29 0.027194311948253662 30-31 0.015428477338937718 32-33 0.02054969460690444 34-35 0.02063072652570138 36-37 0.021619315935024072 38-39 0.02033901161803239 40-41 0.026610882132915684 42-43 0.03604299748087971 44-45 0.03810120821832203 46-47 0.021846205307655506 48-49 0.01341888575277356 50-51 0.013175789996382734 52-53 0.023239954310962905 54-55 0.027145692796975502 56-57 0.020711758444498324 58-59 0.026789152354268955 60-61 0.026562262981637517 62-63 0.052379032310343174 64-65 0.031942782389754454 66-67 0.021441045713670795 68-69 0.02815048859005758 70-71 0.04186108925050013 72-73 0.04032148279335824 74-75 0.0410669764462901 76-77 0.02317512877592535 78-79 0.017859434902845972 80-81 0.022996858554572078 82-83 0.01967454988389747 84-85 0.01957731158134114 86-87 0.02139242656239263 88-89 0.018102530659236796 90-91 0.027583265158478986 92-93 0.027145692796975502 94-95 0.020306598850513613 96-97 0.014601951767208911 98-99 0.019496279662544195 100 0.042493138217116276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3085204.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.59111091488667 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48756274327589 47.963432787034534 2 13.529562386361624 16.124833055911086 3 3.0579416080595685 5.4667841261137395 4 1.0634407109779043 2.53486453437161 5 0.4813565058057984 1.4342284463537813 6 0.27361764291781715 0.97831075844313 7 0.1830462841774182 0.7635551996082075 8 0.1375635148635667 0.6558050137661172 9 0.09957065838025021 0.5340173532665203 >10 0.5627024402104662 6.170428714252958 >50 0.06181944720297201 2.629221755510384 >100 0.0543183670503167 6.609894762673852 >500 0.004498614429953856 1.8651487293711726 >1k 0.002436749482878432 2.353496450334961 >5k 2.499230238849674E-4 0.9555098782620804 >10k+ 3.1240377985620923E-4 2.960468434726047 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20742 0.6723056238744666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17436 0.5651490144573907 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 15345 0.49737391757562865 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13796 0.4471665406890436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12193 0.3952088743564445 No Hit GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7014 0.2273431513766999 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 6533 0.21175261020016828 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6326 0.2050431673237815 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 5790 0.1876699239337172 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3214 0.10417463480534837 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.241276751877672E-5 3 0.0 0.0 0.0 0.0 3.241276751877672E-5 4 0.0 0.0 0.0 0.0 3.241276751877672E-5 5 0.0 0.0 0.0 0.0 3.241276751877672E-5 6 0.0 0.0 0.0 0.0 3.241276751877672E-5 7 0.0 0.0 0.0 0.0 3.241276751877672E-5 8 0.0 0.0 0.0 0.0 3.241276751877672E-5 9 0.0 0.0 0.0 3.241276751877672E-5 3.241276751877672E-5 10-11 0.0 0.0 0.0 9.723830255633015E-5 3.241276751877672E-5 12-13 0.0 0.0 0.0 9.723830255633015E-5 3.241276751877672E-5 14-15 0.0 0.0 0.0 1.2965107007510687E-4 3.241276751877672E-5 16-17 0.0 0.0 0.0 1.458574538344952E-4 3.241276751877672E-5 18-19 0.0 0.0 0.0 1.7827022135327193E-4 6.482553503755344E-5 20-21 0.0 0.0 0.0 2.1068298887204866E-4 6.482553503755344E-5 22-23 0.0 0.0 0.0 5.672234315785925E-4 6.482553503755344E-5 24-25 0.0 1.620638375938836E-5 0.0 0.0011182404793977966 6.482553503755344E-5 26-27 0.0 3.241276751877672E-5 0.0 0.0017340830622545544 6.482553503755344E-5 28-29 0.0 3.241276751877672E-5 0.0 0.003792293799696876 6.482553503755344E-5 30-31 0.0 3.241276751877672E-5 0.0 0.010031751547061395 6.482553503755344E-5 32-33 0.0 3.241276751877672E-5 0.0 0.02437440117412009 6.482553503755344E-5 34-35 0.0 3.241276751877672E-5 0.0 0.043935506371701835 6.482553503755344E-5 36-37 0.0 3.241276751877672E-5 0.0 0.07328526735995416 6.482553503755344E-5 38-39 0.0 6.482553503755344E-5 0.0 0.12853282959570905 6.482553503755344E-5 40-41 0.0 6.482553503755344E-5 0.0 0.21821895732016422 9.723830255633015E-5 42-43 0.0 6.482553503755344E-5 0.0 0.2917473204365092 9.723830255633015E-5 44-45 0.0 6.482553503755344E-5 0.0 0.3704293135883397 9.723830255633015E-5 46-47 0.0 6.482553503755344E-5 0.0 0.4563717666643762 9.723830255633015E-5 48-49 0.0 6.482553503755344E-5 0.0 0.553091464940406 1.6206383759388357E-4 50-51 3.241276751877672E-5 6.482553503755344E-5 0.0 0.6671519938389812 1.6206383759388357E-4 52-53 6.482553503755344E-5 6.482553503755344E-5 0.0 0.7943883127339391 1.6206383759388357E-4 54-55 6.482553503755344E-5 6.482553503755344E-5 0.0 0.954750480033087 1.6206383759388357E-4 56-57 6.482553503755344E-5 6.482553503755344E-5 0.0 1.116717079324414 1.6206383759388357E-4 58-59 6.482553503755344E-5 6.482553503755344E-5 0.0 1.292685994183853 1.7827022135327193E-4 60-61 6.482553503755344E-5 6.482553503755344E-5 0.0 1.4714910262011847 1.944766051126603E-4 62-63 6.482553503755344E-5 6.482553503755344E-5 0.0 1.6781224191333863 1.944766051126603E-4 64-65 6.482553503755344E-5 6.482553503755344E-5 0.0 1.883133173689649 1.944766051126603E-4 66-67 6.482553503755344E-5 6.482553503755344E-5 0.0 2.1092446399006355 2.1068298887204866E-4 68-69 6.482553503755344E-5 6.482553503755344E-5 0.0 2.401834692292633 2.2688937263143702E-4 70-71 6.482553503755344E-5 6.482553503755344E-5 0.0 2.7574999902761697 2.2688937263143702E-4 72-73 6.482553503755344E-5 6.482553503755344E-5 0.0 3.0527640959884663 2.2688937263143702E-4 74-75 6.482553503755344E-5 6.482553503755344E-5 0.0 3.3522418614782037 2.2688937263143702E-4 76-77 6.482553503755344E-5 6.482553503755344E-5 0.0 3.6738899599507846 2.2688937263143702E-4 78-79 6.482553503755344E-5 6.482553503755344E-5 0.0 4.048500520549046 2.2688937263143702E-4 80-81 6.482553503755344E-5 6.482553503755344E-5 0.0 4.408930495357843 2.4309575639082538E-4 82-83 6.482553503755344E-5 6.482553503755344E-5 0.0 4.807899250746466 3.2412767518776714E-4 84-85 6.482553503755344E-5 6.482553503755344E-5 0.0 5.225861887901091 3.2412767518776714E-4 86-87 6.482553503755344E-5 6.482553503755344E-5 0.0 5.651781859481577 3.2412767518776714E-4 88 6.482553503755344E-5 6.482553503755344E-5 0.0 5.991791790753545 3.2412767518776714E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 8765 0.0 73.88401 2 ACCTGGG 9100 0.0 68.99146 3 TATAACG 700 0.0 65.841 2 GTACCTG 10190 0.0 64.239395 1 TAACGCA 785 0.0 59.860386 4 TACCCGG 610 0.0 57.05192 2 GTACCCG 670 0.0 56.23103 1 CCTGGGG 4845 0.0 54.603912 4 ATAACGC 1030 0.0 46.992687 3 TATCACG 345 0.0 43.621372 2 CCTGGGA 6130 0.0 42.161045 4 CTGGGGG 3445 0.0 41.19199 5 GTATAAC 1420 0.0 36.149223 1 ACCCGGG 965 0.0 34.57493 3 ATGGGGG 6555 0.0 32.04276 5 TGGGGGG 8590 0.0 31.9902 6 GTGGTTA 2370 0.0 31.59436 1 CATGGGG 9650 0.0 31.40809 4 TGGGATG 3950 0.0 31.157206 6 GGATGAC 2405 0.0 31.057858 8 >>END_MODULE